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The map label for this gene is ubiB [H]

Identifier: 225630615

GI number: 225630615

Start: 960587

End: 962017

Strand: Direct

Name: ubiB [H]

Synonym: WRi_008860

Alternate gene names: 225630615

Gene position: 960587-962017 (Clockwise)

Preceding gene: 225630614

Following gene: 225630616

Centisome position: 66.44

GC content: 32.7

Gene sequence:

>1431_bases
ATGATTCAAAATATTCTGCGTCTTCTGCACATAACTACGGTGCTGACGCGTTACAATATATTACCTTATTTACTTCCACC
ATCAAAAAAATCAATAAATAAAATACAAGGCCACAAGCTAAAGTGTGCTCTTGAAAAATTAGGTCCGGTGTTCATTAAGT
TTGGGCAGTCTATCTCATCACGTACTGATGTTTTAAATGAAGATATAACAAATAACTTGTTATTGATATGTGATAGATTG
CCATCATTTTCACATAAAATAGCAGTTAAAACTATAGAAAGTGAGTTTAATTGTAAATTGAGCGACATTTTTTCAAGCTT
TTCTGAAAAGCCAATTGCAGCAGCATCGATTTCTCAGGTGCATAGAGCGGTTACAACTGAGGGTAAGGAAGTTGCTGTGA
AGGTTTTAAGGCCAAATATTGAGAAAACATTCTCAAGAGATATAAAAATGCTTTCTTGGCTTGCAGAAATTGCAGAAAAA
TTTAGCGAACAATCAAAAAGGCTGAGGCCAATTGAATTAGTTAAAACTTTTGCTGAAATTTGCCGATTAGAGTTAGATCT
ACGTTTTGAAGCTGCTCACTCTTCGGAACTAAAGGAAAACACAAAAAATGACAGAGGTTTTTATGTACCTGAAATAGATT
GGAATAGAACTGCAAAAAAGGTTTTAACATTAGAATGGATAGAAGCAACGCCAATATACGAAGTTGAAAAACTGAATAAT
CATAAGCAAATAGCTATCAATCTTATAGAATCGTTTTGTAATCAGGTATACAGGGATTGTTTTTTTCATGCTGATATGCA
TCCTGGAAATCTAATGGTTGATAGTAATAACAATATTATTGCTCTGGATTGTGGAATCATGGGTAGAATAGATCGTGAGA
CATGCTATTACGTTATAGAGATACTCAAAGGCTTTTTAAATCGGGATTATGATCACGTTGCAAAAATGCACTTTAAAGCT
GGTTACGTTTCATCACAGCATAGAAATTTTGTCACAGCTTGCAGGGCAATAGGTGAACCCATTATTGGGCAGCCTATACA
GAAGATTTCATTTGCTAGTTTACTTGCTCAGCTATTAAAAATAACTGGTGATTTTGATATGAAAGTTCAAACACAATTGT
TATTGCTGCAGAAAACTATGATTTTACTGGAAGGGACATGTAGAAAGATCTACCCAGAAATCAATATGTGGAAAGTAGTT
GAAGCGTGGATAAGCAGTCAACATGAAAGTAAAATAGGGTATAGGGAAAAAATTAGAAGTTCTTATCCTGTAAAAACAAT
CCAAGGGATATTTAGCCTTATAGAAAAATTGAACCTAATAGCTGATAAAAAATTAGAAAACATCCAAATTAAAAATAAAT
CAAATGGAAAAGTCTATTTTTTATTGTGGTTTGTAATTATAATTCTAATCGTTAAACTTTTAATTTCTTAA

Upstream 100 bases:

>100_bases
ACATAATTTGTATAAATAATACTATCTATTGCCAAGGCAAGCCTGATGAGATTATGAAAAATAGAACCCTAAATGAAATA
TTTAGCAGTTACGCAGCGAA

Downstream 100 bases:

>100_bases
ACTATGGGAAAAAACTGCGTCAAATCCAATTTTTATTCAACTTAATTTTTAATCTGCAGGAGCCGAGTTGAGAATGTCCA
ATTTTCCACTTTCTTTATGT

Product: ubiquinone biosynthesis protein AarF, putative

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MIQNILRLLHITTVLTRYNILPYLLPPSKKSINKIQGHKLKCALEKLGPVFIKFGQSISSRTDVLNEDITNNLLLICDRL
PSFSHKIAVKTIESEFNCKLSDIFSSFSEKPIAAASISQVHRAVTTEGKEVAVKVLRPNIEKTFSRDIKMLSWLAEIAEK
FSEQSKRLRPIELVKTFAEICRLELDLRFEAAHSSELKENTKNDRGFYVPEIDWNRTAKKVLTLEWIEATPIYEVEKLNN
HKQIAINLIESFCNQVYRDCFFHADMHPGNLMVDSNNNIIALDCGIMGRIDRETCYYVIEILKGFLNRDYDHVAKMHFKA
GYVSSQHRNFVTACRAIGEPIIGQPIQKISFASLLAQLLKITGDFDMKVQTQLLLLQKTMILLEGTCRKIYPEINMWKVV
EAWISSQHESKIGYREKIRSSYPVKTIQGIFSLIEKLNLIADKKLENIQIKNKSNGKVYFLLWFVIIILIVKLLIS

Sequences:

>Translated_476_residues
MIQNILRLLHITTVLTRYNILPYLLPPSKKSINKIQGHKLKCALEKLGPVFIKFGQSISSRTDVLNEDITNNLLLICDRL
PSFSHKIAVKTIESEFNCKLSDIFSSFSEKPIAAASISQVHRAVTTEGKEVAVKVLRPNIEKTFSRDIKMLSWLAEIAEK
FSEQSKRLRPIELVKTFAEICRLELDLRFEAAHSSELKENTKNDRGFYVPEIDWNRTAKKVLTLEWIEATPIYEVEKLNN
HKQIAINLIESFCNQVYRDCFFHADMHPGNLMVDSNNNIIALDCGIMGRIDRETCYYVIEILKGFLNRDYDHVAKMHFKA
GYVSSQHRNFVTACRAIGEPIIGQPIQKISFASLLAQLLKITGDFDMKVQTQLLLLQKTMILLEGTCRKIYPEINMWKVV
EAWISSQHESKIGYREKIRSSYPVKTIQGIFSLIEKLNLIADKKLENIQIKNKSNGKVYFLLWFVIIILIVKLLIS
>Mature_476_residues
MIQNILRLLHITTVLTRYNILPYLLPPSKKSINKIQGHKLKCALEKLGPVFIKFGQSISSRTDVLNEDITNNLLLICDRL
PSFSHKIAVKTIESEFNCKLSDIFSSFSEKPIAAASISQVHRAVTTEGKEVAVKVLRPNIEKTFSRDIKMLSWLAEIAEK
FSEQSKRLRPIELVKTFAEICRLELDLRFEAAHSSELKENTKNDRGFYVPEIDWNRTAKKVLTLEWIEATPIYEVEKLNN
HKQIAINLIESFCNQVYRDCFFHADMHPGNLMVDSNNNIIALDCGIMGRIDRETCYYVIEILKGFLNRDYDHVAKMHFKA
GYVSSQHRNFVTACRAIGEPIIGQPIQKISFASLLAQLLKITGDFDMKVQTQLLLLQKTMILLEGTCRKIYPEINMWKVV
EAWISSQHESKIGYREKIRSSYPVKTIQGIFSLIEKLNLIADKKLENIQIKNKSNGKVYFLLWFVIIILIVKLLIS

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis [H]

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40254938, Length=254, Percent_Identity=26.3779527559055, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI41393593, Length=286, Percent_Identity=24.8251748251748, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI34147522, Length=282, Percent_Identity=27.6595744680851, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI217035081, Length=209, Percent_Identity=26.7942583732057, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI27363457, Length=269, Percent_Identity=26.7657992565056, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI217416386, Length=269, Percent_Identity=26.7657992565056, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI2367309, Length=418, Percent_Identity=35.6459330143541, Blast_Score=275, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI17559152, Length=249, Percent_Identity=31.7269076305221, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32565180, Length=214, Percent_Identity=24.7663551401869, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6325148, Length=495, Percent_Identity=20.8080808080808, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323282, Length=247, Percent_Identity=22.6720647773279, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24662575, Length=248, Percent_Identity=29.8387096774194, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI22024280, Length=384, Percent_Identity=24.4791666666667, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859849, Length=298, Percent_Identity=21.8120805369128, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR010232 [H]

Pfam domain/function: PF03109 ABC1 [H]

EC number: NA

Molecular weight: Translated: 54751; Mature: 54751

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQNILRLLHITTVLTRYNILPYLLPPSKKSINKIQGHKLKCALEKLGPVFIKFGQSISS
CHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHCCCHHHHHHHHHCHHHHHHCCCHHH
RTDVLNEDITNNLLLICDRLPSFSHKIAVKTIESEFNCKLSDIFSSFSEKPIAAASISQV
HHHHHHHHHCCCEEEEECCCCCCCHHEEEEEECCHHCCCHHHHHHHHCCCCCHHHHHHHH
HRAVTTEGKEVAVKVLRPNIEKTFSRDIKMLSWLAEIAEKFSEQSKRLRPIELVKTFAEI
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
CRLELDLRFEAAHSSELKENTKNDRGFYVPEIDWNRTAKKVLTLEWIEATPIYEVEKLNN
HHHHHCEEEECCCHHHHHHCCCCCCCEECCCCCCCHHHHHHEEEEEECCCCCHHHHHCCC
HKQIAINLIESFCNQVYRDCFFHADMHPGNLMVDSNNNIIALDCGIMGRIDRETCYYVIE
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHH
ILKGFLNRDYDHVAKMHFKAGYVSSQHRNFVTACRAIGEPIIGQPIQKISFASLLAQLLK
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ITGDFDMKVQTQLLLLQKTMILLEGTCRKIYPEINMWKVVEAWISSQHESKIGYREKIRS
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCCHHHHHCC
SYPVKTIQGIFSLIEKLNLIADKKLENIQIKNKSNGKVYFLLWFVIIILIVKLLIS
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIQNILRLLHITTVLTRYNILPYLLPPSKKSINKIQGHKLKCALEKLGPVFIKFGQSISS
CHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHCCCHHHHHHHHHCHHHHHHCCCHHH
RTDVLNEDITNNLLLICDRLPSFSHKIAVKTIESEFNCKLSDIFSSFSEKPIAAASISQV
HHHHHHHHHCCCEEEEECCCCCCCHHEEEEEECCHHCCCHHHHHHHHCCCCCHHHHHHHH
HRAVTTEGKEVAVKVLRPNIEKTFSRDIKMLSWLAEIAEKFSEQSKRLRPIELVKTFAEI
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
CRLELDLRFEAAHSSELKENTKNDRGFYVPEIDWNRTAKKVLTLEWIEATPIYEVEKLNN
HHHHHCEEEECCCHHHHHHCCCCCCCEECCCCCCCHHHHHHEEEEEECCCCCHHHHHCCC
HKQIAINLIESFCNQVYRDCFFHADMHPGNLMVDSNNNIIALDCGIMGRIDRETCYYVIE
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHH
ILKGFLNRDYDHVAKMHFKAGYVSSQHRNFVTACRAIGEPIIGQPIQKISFASLLAQLLK
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ITGDFDMKVQTQLLLLQKTMILLEGTCRKIYPEINMWKVVEAWISSQHESKIGYREKIRS
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCCHHHHHCC
SYPVKTIQGIFSLIEKLNLIADKKLENIQIKNKSNGKVYFLLWFVIIILIVKLLIS
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA