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The map label for this gene is pyrB

Identifier: 225630582

GI number: 225630582

Start: 920560

End: 921447

Strand: Direct

Name: pyrB

Synonym: WRi_008480

Alternate gene names: 225630582

Gene position: 920560-921447 (Clockwise)

Preceding gene: 225630580

Following gene: 225630583

Centisome position: 63.67

GC content: 35.36

Gene sequence:

>888_bases
ATGAACAAGAGAAGGAATTTGTTAAACATCTCAGACCTTACAGTCGATGATGTAGAAAATATAACTAAGTTGGCCAACCA
GTACCTTAAAAAAGAAGTTGCAAATAGTCATATCTTGGAAAATAAGACAGTAATAAATTTATTCTTTGAAGATTCAACGC
GTACACTTGCGTCTTTTGAAATAGCAGCAAAAAGCCTTGGAGCAAATGTTGTAACTTTGCCAATAAGATCTTCTTCTATT
AACAAGGGAGAAGATCTAAAAGACATGATCAAAACATTAAATGCAATGAATCCTGATTACATAATAATCAGGCATAAAAG
TAGTGGTATCATTAATACACTGGCAAAGTATGTTAACTGCTCGCTAATCAATGCAGGCGATGGAAGTAGTGAACATCCAA
CTCAAGCTCTTGCAGACTATCTTGTAATCAGCAATCACAAAAAGCAAATAAAGGACCTCAAAGTTGTGATATGTGGAGAT
ATTCTGCACAGTAGAGTTGCAAGATCAAATATAAGATTGCTAAAAATGTTTGGAGCAGAGATAAACTTGGTCGCACCACC
AACTTTGATTTGTAAGCATTTTCCTGAAGTAGATTCAGTCCATTATTCATTAATTGAAGGTATAAAGGATGCTGACGTAA
TTATGCTTTTGAGATTGCAGAAAGAGCGCATGAATAATAGTTCTTCAGAGAAAGAATATTTTTATTTGTATGGACTTGAT
TCACAAAAGCTATCATACGCAAAACCAGATGCGATTGTTATGCACCCAGGGCCAATAAACAGAGGGATTGAGATTAGCAG
CGATGTAGCAGATTGTGTTATTTTACAGCAAGTAGAATTTGGATTGGCAATACGTAAGGCAGTGTTGCACTATTATAGGC
CTTGCTAA

Upstream 100 bases:

>100_bases
TCTTTTTCCGTTCTTTCACCAAAACAATTGCTGCACAAGAAGTCTATTGAAAAAAGGTAATAAGAAAGTAGAATAGGTTC
AAGTAGTTGAAAAGGAAACT

Downstream 100 bases:

>100_bases
TATTAAAAGATGCTACACAACTAATGCAAAAAGGGGGGGCATGAAATGACAAGGCTTGTAAATGGTTTGAGAATGGCCAT
TAAGATGTTAAGGCCGAGGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MNKRRNLLNISDLTVDDVENITKLANQYLKKEVANSHILENKTVINLFFEDSTRTLASFEIAAKSLGANVVTLPIRSSSI
NKGEDLKDMIKTLNAMNPDYIIIRHKSSGIINTLAKYVNCSLINAGDGSSEHPTQALADYLVISNHKKQIKDLKVVICGD
ILHSRVARSNIRLLKMFGAEINLVAPPTLICKHFPEVDSVHYSLIEGIKDADVIMLLRLQKERMNNSSSEKEYFYLYGLD
SQKLSYAKPDAIVMHPGPINRGIEISSDVADCVILQQVEFGLAIRKAVLHYYRPC

Sequences:

>Translated_295_residues
MNKRRNLLNISDLTVDDVENITKLANQYLKKEVANSHILENKTVINLFFEDSTRTLASFEIAAKSLGANVVTLPIRSSSI
NKGEDLKDMIKTLNAMNPDYIIIRHKSSGIINTLAKYVNCSLINAGDGSSEHPTQALADYLVISNHKKQIKDLKVVICGD
ILHSRVARSNIRLLKMFGAEINLVAPPTLICKHFPEVDSVHYSLIEGIKDADVIMLLRLQKERMNNSSSEKEYFYLYGLD
SQKLSYAKPDAIVMHPGPINRGIEISSDVADCVILQQVEFGLAIRKAVLHYYRPC
>Mature_295_residues
MNKRRNLLNISDLTVDDVENITKLANQYLKKEVANSHILENKTVINLFFEDSTRTLASFEIAAKSLGANVVTLPIRSSSI
NKGEDLKDMIKTLNAMNPDYIIIRHKSSGIINTLAKYVNCSLINAGDGSSEHPTQALADYLVISNHKKQIKDLKVVICGD
ILHSRVARSNIRLLKMFGAEINLVAPPTLICKHFPEVDSVHYSLIEGIKDADVIMLLRLQKERMNNSSSEKEYFYLYGLD
SQKLSYAKPDAIVMHPGPINRGIEISSDVADCVILQQVEFGLAIRKAVLHYYRPC

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=299, Percent_Identity=30.7692307692308, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI2367364, Length=303, Percent_Identity=32.3432343234323, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790703, Length=226, Percent_Identity=29.646017699115, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786469, Length=228, Percent_Identity=29.8245614035088, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=316, Percent_Identity=29.4303797468354, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6322331, Length=309, Percent_Identity=33.9805825242718, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24642586, Length=291, Percent_Identity=32.3024054982818, Blast_Score=137, Evalue=9e-33,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_WOLPM (Q73GP1)

Other databases:

- EMBL:   AE017196
- RefSeq:   NP_966641.1
- ProteinModelPortal:   Q73GP1
- SMR:   Q73GP1
- EnsemblBacteria:   EBWOLT00000004569
- GeneID:   2737722
- GenomeReviews:   AE017196_GR
- KEGG:   wol:WD0895
- TIGR:   WD_0895
- GeneTree:   EBGT00050000031220
- HOGENOM:   HBG579429
- OMA:   LYFAGPE
- ProtClustDB:   PRK00856
- BioCyc:   WPIP955:WD_0895-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33056; Mature: 33056

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRRNLLNISDLTVDDVENITKLANQYLKKEVANSHILENKTVINLFFEDSTRTLASFE
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEECCCCHHHHHH
IAAKSLGANVVTLPIRSSSINKGEDLKDMIKTLNAMNPDYIIIRHKSSGIINTLAKYVNC
HHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCC
SLINAGDGSSEHPTQALADYLVISNHKKQIKDLKVVICGDILHSRVARSNIRLLKMFGAE
EEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHEEEEEEHHHHHHHHHHCCHHEEEECCCE
INLVAPPTLICKHFPEVDSVHYSLIEGIKDADVIMLLRLQKERMNNSSSEKEYFYLYGLD
EEEECCHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECC
SQKLSYAKPDAIVMHPGPINRGIEISSDVADCVILQQVEFGLAIRKAVLHYYRPC
CCCCCCCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKRRNLLNISDLTVDDVENITKLANQYLKKEVANSHILENKTVINLFFEDSTRTLASFE
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEECCCCHHHHHH
IAAKSLGANVVTLPIRSSSINKGEDLKDMIKTLNAMNPDYIIIRHKSSGIINTLAKYVNC
HHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCC
SLINAGDGSSEHPTQALADYLVISNHKKQIKDLKVVICGDILHSRVARSNIRLLKMFGAE
EEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHEEEEEEHHHHHHHHHHCCHHEEEECCCE
INLVAPPTLICKHFPEVDSVHYSLIEGIKDADVIMLLRLQKERMNNSSSEKEYFYLYGLD
EEEECCHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECC
SQKLSYAKPDAIVMHPGPINRGIEISSDVADCVILQQVEFGLAIRKAVLHYYRPC
CCCCCCCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA