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The map label for this gene is truB [H]

Identifier: 225630578

GI number: 225630578

Start: 914190

End: 914450

Strand: Reverse

Name: truB [H]

Synonym: WRi_008410

Alternate gene names: 225630578

Gene position: 914450-914190 (Counterclockwise)

Preceding gene: 225630581

Following gene: 225630577

Centisome position: 63.25

GC content: 34.87

Gene sequence:

>261_bases
ATGACAGAAGATGATGGAGTGACAGGGAGTAATACAAAAAGTTTCGTCATCCCCATTGAATCGGCTTTAAAATCGATGTT
CAAAGTTGAAATTTCCCTAGAAGATGCGGGGAAAATCAGAAAAGGTCAGGAAGTTGTATTAAATAACTTGCGTAATTTAA
AGAATCATGATATTTGTTGTACGGTGGTGGGTAGTGTACCTATTGCAATCTGCAGTTTTATTTATGGTTGTGTGAAACCT
ATTCGTGTTTTTAATATTTAA

Upstream 100 bases:

>100_bases
GAGATAGCTTCATAAATTTATTGTGCGTTACCTACTATCCGAAGAGCGATGCAACAAAGCCATCCCAGTGTCAGCTACTT
GGATGACAGAGGGGAGTGGG

Downstream 100 bases:

>100_bases
AATGAGGTTTAGATGTCGATAACATCCGAAAAGAAAAAGAGTTTGATAAATATATATGCAATTAAAGAAGATGATACAGG
TTCATCTTTTGTACAATGTG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 86; Mature: 85

Protein sequence:

>86_residues
MTEDDGVTGSNTKSFVIPIESALKSMFKVEISLEDAGKIRKGQEVVLNNLRNLKNHDICCTVVGSVPIAICSFIYGCVKP
IRVFNI

Sequences:

>Translated_86_residues
MTEDDGVTGSNTKSFVIPIESALKSMFKVEISLEDAGKIRKGQEVVLNNLRNLKNHDICCTVVGSVPIAICSFIYGCVKP
IRVFNI
>Mature_85_residues
TEDDGVTGSNTKSFVIPIESALKSMFKVEISLEDAGKIRKGQEVVLNNLRNLKNHDICCTVVGSVPIAICSFIYGCVKPI
RVFNI

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: NA

Molecular weight: Translated: 9416; Mature: 9285

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDDGVTGSNTKSFVIPIESALKSMFKVEISLEDAGKIRKGQEVVLNNLRNLKNHDICC
CCCCCCCCCCCCCEEEEEHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEE
TVVGSVPIAICSFIYGCVKPIRVFNI
EECCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEDDGVTGSNTKSFVIPIESALKSMFKVEISLEDAGKIRKGQEVVLNNLRNLKNHDICC
CCCCCCCCCCCCEEEEEHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEE
TVVGSVPIAICSFIYGCVKPIRVFNI
EECCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA