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The map label for this gene is ppnK [H]

Identifier: 225630574

GI number: 225630574

Start: 907538

End: 908332

Strand: Direct

Name: ppnK [H]

Synonym: WRi_008360

Alternate gene names: 225630574

Gene position: 907538-908332 (Clockwise)

Preceding gene: 225630573

Following gene: 225630575

Centisome position: 62.77

GC content: 34.34

Gene sequence:

>795_bases
ATGCATAAATACAAAAATATAGGCTATGTTGCTTCTCCATCACCAAAATCGCAGGAAGTATCTAAACTATTAAAGAAACT
TAATTTTATCAATATAACAGAAGAAAATAAGTCCGAAATTGATCTACTGATAGTTGTTGGCGGTGATGGCTTTATGCTGC
GTACCTTGCATAATTACGTTATAGAAAATAAAAACATGCATGTGTATGGGATAAACACTGGCAATGTTGGGTTTTTGATG
AATAAATGCTTTAGCTGCAGTGAAGATTTAATTGATCATATAGAGCATGCAACTTCAACTCAGTTAACTTTGCTAAAAAT
GGAAGCAACAGACACAAGTGGCAAGAGATATCATTACATAGCAGTAAACGAAGTATACGTTTTTAGAAAAGCGAACCAAA
TAGTAGAGATGAATATTACTATCAACGATAAGCTAAAAGTAGAGAAATTTAGAGGGGACGGAGTAATATTATCTACCCCC
ACAGGCAGCACTGCATACAACTTCTCTGCCGGTGGCCCAATCTTGCCGCTAAATTCAAATTTACTTGCATTAACCTCTAT
CAATAGCTATTACCCAAGGCATTGGAATGGAGCGTTAATATCAAACGATACAATCGTACAAATTGACATTAACGACACAA
AAAACCGTCCAGCACTTGTAGTATCAGATTACAAAGAATTTCATGATATATCACAGATAAAAATACAAAAAGACCATGAG
AACACAATCACTTTGCTTTTTGACAAAGATTACCCTCTGAATGAAAGAATCTTTGATAGACAATTTCTATACTAA

Upstream 100 bases:

>100_bases
GGTTAGCAAGTAAAACTAGCAAATTAGCTAAGTTTAACGATAAATATGGAAGCATTTTCTAATTCTCCTCCTCTTTAAGT
TTTAGAGAGTTTACTGAAGC

Downstream 100 bases:

>100_bases
TAATTATCCGTAAATTGGTATTTACTATATCTTAATAACTATAGCGTAATCAATGCAGCATAGTAATTAATTTTGGAGTG
AATATGCCTGGACCAGTAAA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MHKYKNIGYVASPSPKSQEVSKLLKKLNFINITEENKSEIDLLIVVGGDGFMLRTLHNYVIENKNMHVYGINTGNVGFLM
NKCFSCSEDLIDHIEHATSTQLTLLKMEATDTSGKRYHYIAVNEVYVFRKANQIVEMNITINDKLKVEKFRGDGVILSTP
TGSTAYNFSAGGPILPLNSNLLALTSINSYYPRHWNGALISNDTIVQIDINDTKNRPALVVSDYKEFHDISQIKIQKDHE
NTITLLFDKDYPLNERIFDRQFLY

Sequences:

>Translated_264_residues
MHKYKNIGYVASPSPKSQEVSKLLKKLNFINITEENKSEIDLLIVVGGDGFMLRTLHNYVIENKNMHVYGINTGNVGFLM
NKCFSCSEDLIDHIEHATSTQLTLLKMEATDTSGKRYHYIAVNEVYVFRKANQIVEMNITINDKLKVEKFRGDGVILSTP
TGSTAYNFSAGGPILPLNSNLLALTSINSYYPRHWNGALISNDTIVQIDINDTKNRPALVVSDYKEFHDISQIKIQKDHE
NTITLLFDKDYPLNERIFDRQFLY
>Mature_264_residues
MHKYKNIGYVASPSPKSQEVSKLLKKLNFINITEENKSEIDLLIVVGGDGFMLRTLHNYVIENKNMHVYGINTGNVGFLM
NKCFSCSEDLIDHIEHATSTQLTLLKMEATDTSGKRYHYIAVNEVYVFRKANQIVEMNITINDKLKVEKFRGDGVILSTP
TGSTAYNFSAGGPILPLNSNLLALTSINSYYPRHWNGALISNDTIVQIDINDTKNRPALVVSDYKEFHDISQIKIQKDHE
NTITLLFDKDYPLNERIFDRQFLY

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=164, Percent_Identity=37.1951219512195, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320794, Length=218, Percent_Identity=29.8165137614679, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322509, Length=165, Percent_Identity=31.5151515151515, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 30087; Mature: 30087

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKYKNIGYVASPSPKSQEVSKLLKKLNFINITEENKSEIDLLIVVGGDGFMLRTLHNYV
CCCCCCCCEEECCCCCHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCEEHHHHHHHH
IENKNMHVYGINTGNVGFLMNKCFSCSEDLIDHIEHATSTQLTLLKMEATDTSGKRYHYI
EECCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEE
AVNEVYVFRKANQIVEMNITINDKLKVEKFRGDGVILSTPTGSTAYNFSAGGPILPLNSN
EEEEEEEEEECCEEEEEEEEECCCEEEEEECCCEEEEECCCCCCEEEECCCCCEEECCCC
LLALTSINSYYPRHWNGALISNDTIVQIDINDTKNRPALVVSDYKEFHDISQIKIQKDHE
EEEEEECCCCCCCCCCCEEECCCEEEEEECCCCCCCCEEEEECCHHHCCCEEEEEEECCC
NTITLLFDKDYPLNERIFDRQFLY
CEEEEEEECCCCCCCHHCCCCCCC
>Mature Secondary Structure
MHKYKNIGYVASPSPKSQEVSKLLKKLNFINITEENKSEIDLLIVVGGDGFMLRTLHNYV
CCCCCCCCEEECCCCCHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCEEHHHHHHHH
IENKNMHVYGINTGNVGFLMNKCFSCSEDLIDHIEHATSTQLTLLKMEATDTSGKRYHYI
EECCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEE
AVNEVYVFRKANQIVEMNITINDKLKVEKFRGDGVILSTPTGSTAYNFSAGGPILPLNSN
EEEEEEEEEECCEEEEEEEEECCCEEEEEECCCEEEEECCCCCCEEEECCCCCEEECCCC
LLALTSINSYYPRHWNGALISNDTIVQIDINDTKNRPALVVSDYKEFHDISQIKIQKDHE
EEEEEECCCCCCCCCCCEEECCCEEEEEECCCCCCCCEEEEECCHHHCCCEEEEEEECCC
NTITLLFDKDYPLNERIFDRQFLY
CEEEEEEECCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]