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The map label for this gene is fmt [H]

Identifier: 225630569

GI number: 225630569

Start: 901392

End: 902291

Strand: Direct

Name: fmt [H]

Synonym: WRi_008300

Alternate gene names: 225630569

Gene position: 901392-902291 (Clockwise)

Preceding gene: 225630568

Following gene: 225630570

Centisome position: 62.34

GC content: 37.67

Gene sequence:

>900_bases
ATGAGAATTATTTTCATGGGATCACCAGGATTTGCTGTTAATTCTCTGAGCTTGTTACTGAAATCAAAGAGCGAAGTGGT
GGCAGTATACACCAAGGCTCCAAAACCTTCGGGGCGTGGACAGAAGCCAATGAAATCTCCAGTACATGTTATCGCTGAAG
AAAGTAACATAGAGGTATGTACTCCTATCTCTCTAAAGTTCTCGGCAGAGCAAGAAAAATTTAGAAATTTCAAGCCAGAC
GTTGCGGTTGTTGCCGCGTATGGATTGATACTTCCAAGAGAAATTTTGAATATTCCAAAATATGGTTGTATTAATATTCA
TCCTTCATTACTACCGAGGTGGCGCGGTGCAGCTCCGATACAGCACACAATTTTAGCAGGAGATCAAGAAACCGGGGTTA
GCATTATGCAACTGGATGAAGGATTAGATTCTGGCCCTATTTTAAAACAGGAAAAATTTCTTATCGAAAAGAACGATAAC
TACAAGACATTGCATGATAAATTATCTAAGCTAGGCAGTGATTTACTGCTGAAAGTGCTAAACGAAATTGAAAAACAGCT
TCCCTTAAAACAGAACGATAACGATGCATGTTACGCTGACAAAGTGGAAGACTATAAAATTTATGCAAGTGATGCCTGTG
AAGTTGCTTATAGAAAGGTTAAAGCGTTTTACCCAAAAGCGTTTATCAAGATAGAGAATAAACGTATCAGGATACTTGAT
GCTGACTTTGAAGCTCTCGCTTCAGAACAAGGTAAGATTGTTAACGATAATATGCACATAAGTTTAAAAGGTGGCACTTT
AATTCCTAAAGTTGTACAAATGGAAGGGAGAAATCCTTGCAGTATTGAAGATTTTATTCGTGGCTTGAAATCAAGCATGG
TAAAAAAATTTATAGAATAG

Upstream 100 bases:

>100_bases
AATTCTTGCTGAGATGGCAGTTAATTATAAGGATGATTTTGCCAAATTAGTAGAAACTGTAAGTGGTAAATTAGCGGAGA
ATTCTTAAAGCAAAAACACC

Downstream 100 bases:

>100_bases
GCTGCTTTAAATTAGTGTAGAAATGTAATAGCTAAATTTCCTTTTCTTAGATATAATCTTAAAAAACTTTCCATATAAAA
ATGAAAATAAAAAGAGCTTT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRIIFMGSPGFAVNSLSLLLKSKSEVVAVYTKAPKPSGRGQKPMKSPVHVIAEESNIEVCTPISLKFSAEQEKFRNFKPD
VAVVAAYGLILPREILNIPKYGCINIHPSLLPRWRGAAPIQHTILAGDQETGVSIMQLDEGLDSGPILKQEKFLIEKNDN
YKTLHDKLSKLGSDLLLKVLNEIEKQLPLKQNDNDACYADKVEDYKIYASDACEVAYRKVKAFYPKAFIKIENKRIRILD
ADFEALASEQGKIVNDNMHISLKGGTLIPKVVQMEGRNPCSIEDFIRGLKSSMVKKFIE

Sequences:

>Translated_299_residues
MRIIFMGSPGFAVNSLSLLLKSKSEVVAVYTKAPKPSGRGQKPMKSPVHVIAEESNIEVCTPISLKFSAEQEKFRNFKPD
VAVVAAYGLILPREILNIPKYGCINIHPSLLPRWRGAAPIQHTILAGDQETGVSIMQLDEGLDSGPILKQEKFLIEKNDN
YKTLHDKLSKLGSDLLLKVLNEIEKQLPLKQNDNDACYADKVEDYKIYASDACEVAYRKVKAFYPKAFIKIENKRIRILD
ADFEALASEQGKIVNDNMHISLKGGTLIPKVVQMEGRNPCSIEDFIRGLKSSMVKKFIE
>Mature_299_residues
MRIIFMGSPGFAVNSLSLLLKSKSEVVAVYTKAPKPSGRGQKPMKSPVHVIAEESNIEVCTPISLKFSAEQEKFRNFKPD
VAVVAAYGLILPREILNIPKYGCINIHPSLLPRWRGAAPIQHTILAGDQETGVSIMQLDEGLDSGPILKQEKFLIEKNDN
YKTLHDKLSKLGSDLLLKVLNEIEKQLPLKQNDNDACYADKVEDYKIYASDACEVAYRKVKAFYPKAFIKIENKRIRILD
ADFEALASEQGKIVNDNMHISLKGGTLIPKVVQMEGRNPCSIEDFIRGLKSSMVKKFIE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI164663775, Length=319, Percent_Identity=31.974921630094, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI238814322, Length=248, Percent_Identity=27.0161290322581, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI21614513, Length=178, Percent_Identity=32.0224719101124, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1789683, Length=305, Percent_Identity=38.6885245901639, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788589, Length=256, Percent_Identity=26.953125, Blast_Score=98, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI133930964, Length=175, Percent_Identity=34.2857142857143, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319458, Length=279, Percent_Identity=26.5232974910394, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45550868, Length=315, Percent_Identity=30.1587301587302, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28571984, Length=237, Percent_Identity=32.9113924050633, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585660, Length=179, Percent_Identity=30.7262569832402, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 33432; Mature: 33432

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIFMGSPGFAVNSLSLLLKSKSEVVAVYTKAPKPSGRGQKPMKSPVHVIAEESNIEVC
CEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEE
TPISLKFSAEQEKFRNFKPDVAVVAAYGLILPREILNIPKYGCINIHPSLLPRWRGAAPI
CCEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCCCCCCC
QHTILAGDQETGVSIMQLDEGLDSGPILKQEKFLIEKNDNYKTLHDKLSKLGSDLLLKVL
EEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
NEIEKQLPLKQNDNDACYADKVEDYKIYASDACEVAYRKVKAFYPKAFIKIENKRIRILD
HHHHHHCCCCCCCCCCEEECCCCCEEEEECHHHHHHHHHHHHHCCEEEEEECCCEEEEEE
ADFEALASEQGKIVNDNMHISLKGGTLIPKVVQMEGRNPCSIEDFIRGLKSSMVKKFIE
CCHHHHHCCCCCEECCCEEEEEECCEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIIFMGSPGFAVNSLSLLLKSKSEVVAVYTKAPKPSGRGQKPMKSPVHVIAEESNIEVC
CEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEE
TPISLKFSAEQEKFRNFKPDVAVVAAYGLILPREILNIPKYGCINIHPSLLPRWRGAAPI
CCEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCCCCCCC
QHTILAGDQETGVSIMQLDEGLDSGPILKQEKFLIEKNDNYKTLHDKLSKLGSDLLLKVL
EEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
NEIEKQLPLKQNDNDACYADKVEDYKIYASDACEVAYRKVKAFYPKAFIKIENKRIRILD
HHHHHHCCCCCCCCCCEEECCCCCEEEEECHHHHHHHHHHHHHCCEEEEEECCCEEEEEE
ADFEALASEQGKIVNDNMHISLKGGTLIPKVVQMEGRNPCSIEDFIRGLKSSMVKKFIE
CCHHHHHCCCCCEECCCEEEEEECCEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA