| Definition | Wolbachia sp. wRi, complete genome. |
|---|---|
| Accession | NC_012416 |
| Length | 1,445,873 |
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The map label for this gene is alaS [H]
Identifier: 225630565
GI number: 225630565
Start: 897437
End: 900079
Strand: Reverse
Name: alaS [H]
Synonym: WRi_008260
Alternate gene names: 225630565
Gene position: 900079-897437 (Counterclockwise)
Preceding gene: 225630572
Following gene: 225630563
Centisome position: 62.25
GC content: 36.63
Gene sequence:
>2643_bases ATGAAGCTAAACGAAATCAGAGAAAGATTTATAAAATTTTTTGTAAATAATGACCACGAGCAGGTTTCTTCTTCTCCTTT GATTCCAGAACATGACCCAACGCTCATGTTCACAAATGCTGGTATGGTGCAGTTTAAAAATATTTTTACTGGTGCTCAAA AAACTGAAATGAAACGTGCTGTCTCAAGTCAAAAGTGCCTAAGAGCAGGCGGTAAACACAATGATCTTGAAAATGTTGGC TATACAACTCGGCATCACACATTTTTTGAAATGCTCGGAAATTTTAGCTTCGGTGATTACTTTAAGGAAACTGCGATAGA ATTTGCGTGGGAATTTATCACTAAAGAGTTATCTCTTGATAAAAACAGACTATCCATAACCGTCTACCATACTGATGATG AGGCGTACGAGATTTGGCGTAAGATAAGTGGCTTTTCAAGTGATAAAATCATAAGAATTACAACGGATGATAACTTTTGG AGTATGGGAAATACTGGTCCGTGCGGTCCATGTTCTGAAATTTTTTATGACCATGGGAGTCCTAATTTACAAGAGGGCGA TAGAATTGTTGAAATCTGGAATCTGGTATTCATGGAGTTTAACAAAGATGAAGAAGGTAATTTACACAAATTGCCAAAAA AATGCATCGATACTGGAATGGGCCTTGAAAGAATAGCGGCTGTTATGCAAAACGTCCATGATAACTATGATATTGATCTA TTTTCTGCTCTGATAAGTAAATCACAAGAGTACTGTGGAAGAACGGAAAATAAAATAGCGCATAAGATCATAGCAGATCA TCTTCGCGCGGCTGCATTTCTCATCGCAGAGGGGGTGCTCCCTGGAAATGAAGGCAGGAATTACGTATTACGCAGATTAA TTAGGAGAGCCGCGCGTTATATCCACCTGCTTGGATATAATGATTCCCTACTCCATCGCGTTTTTCCGGTGCTGATAGAT AGCACGAGTTCGGCTTATATGGGGGATATTTATCCTGAATTGATCAGAGCTAAAAGCTTAATAGAAACGACGTTAAAATC AGAGGAAGAAAACTTTAAAGACACTTTGATGAAAGGCATTAGTCTATTGGAAAAATTCACTGCAGATTTAAAATCGGGCG ATACTTTGCCTGGAGAATCAGCGTTTAAGCTATATGACACCTACGGATTTCCTTTGGATATTACACTTGATATTTTAAAA GAGAAAAAAATAAATTTTGACCAGAAGGGTTTTGATGATGCAATGGGAGAACAAAAAGAGAGAGCACGTGCTAAATGGGC TGGATCTGGTGAAAAATCTGTTGAGCAAGTATGGTTTGATTTGATCGATAAATTTGGCAAAACAAAATTTGTTGGTTATG AGTTCAATGAAGTAAATGATGCGAAAATACTAGCTATAGTTTCCTCTAAAAATGAAGTAATTGATTCTGCAAAGGAGGGA GAAAAGATAACCATTATACTTGATAAAACACCTTTTTATGGCGAGTCAGGTGGACAAGTGGGAGATACTGGCAGTCTGAT CAAATCTGATAGAAGTATTATTATAGTCGAGAATACCAATAAGGTTAATGACCTATATTTGCACAGATGCATAGTTAAGT TCGGTTCAATTTGTAAAGGTAACACAGTTGCAGCAAGCATTGACAAGGAAAGAAGGCAAACCTTAAGAAGAAATCATTCA GCTACACACCTTTTGCACTTTGTATTGAGAAAAATCTTGGGTGATCATGTCACTCAAAAAGGTTCCCTAGTTGCACCGGA TAAGTTACGATTTGACTTTAGTCATAACACTCAAGTTACTCAGGATCAGCTATTCTTAGTAGAAGATATGGTAAACTCTC TAATCAGAGAAAACCTTTCTACGTCTACAAAAATTCAGAGCATGAATCAGGCAATAGACGAAGGAGCTATGGCGTTATTC GGTGAAAAATATGGCAACCAAGTAAGAGTAGTGAAGATTGGAGATTCAAGGGAACTGTGTGGTGGCACACACGTTGAACA TACTGGAGAAATCGGTTTATTTAAGATAGTAACAGAAAGTTCTGTTGCTTTTGGAGTGAGAAGAATTGAAGCTTTAACTG GTCAAGAAGCAATCAATTATGTACGTGATAATGAAATTAACTTAAAAAAAGTTGCAGAATTCGTAAAAGCGCCAGTGAGT GAAATAATAAACCGATTAAATATTTTAAATCAAGAGCGCAAAGAATCTGAAGCTAAAATAAAAAATTTATATAAAAAACT TATAAGCGCAGAGAGCATAAAAAGCACTGAAATAAATGGAATAAATTTTGTAAGTCATGCTTTTACCAATATTCCAGCGA ATATAATAAGAGAGTTTGTTTTGCAGCAACAAAAACCAAAAACGATAATAGCTTTCACGGCAACAGAAAAAGATAAAACA GTCTTGATTATTAAAGTAAGTAAAGACTTAGCTAATAAAATTAGTGCAAAAGAACTAGTATCAATAGCAGTTGAAAAAAA TTGTGGTGGGAACGCTGAACTTGCACAAACAGGCTGTGATAGCAATAAAATAGATGATGCCATTACAGCCATTTGTAGCA AAATAGCAGCTTCTAAGCACTCTACGTCATACCACCACGAACCGTCATACCGCCGCGGCGCTAACAAGCAGCGGGATACT TAA
Upstream 100 bases:
>100_bases ACCACAAGGTGCATGTCACTAGAAGTTCTGAGATCTAGAGAGTGGTATTGATTTTATTTCCATTACAACTTATTATTTAT AGATAACCTCAATAAAAACG
Downstream 100 bases:
>100_bases CCATCGTCTACACCGTCATACCGCGATTCATTCGCGGGATCTCTTAGCCGCTAACAAGAGATCCCGCTAACAAGCAGCGG GATGACGAATTACTATGCCG
Product: alanyl-tRNA synthetase
Products: NA
Alternate protein names: Alanine--tRNA ligase; AlaRS [H]
Number of amino acids: Translated: 880; Mature: 880
Protein sequence:
>880_residues MKLNEIRERFIKFFVNNDHEQVSSSPLIPEHDPTLMFTNAGMVQFKNIFTGAQKTEMKRAVSSQKCLRAGGKHNDLENVG YTTRHHTFFEMLGNFSFGDYFKETAIEFAWEFITKELSLDKNRLSITVYHTDDEAYEIWRKISGFSSDKIIRITTDDNFW SMGNTGPCGPCSEIFYDHGSPNLQEGDRIVEIWNLVFMEFNKDEEGNLHKLPKKCIDTGMGLERIAAVMQNVHDNYDIDL FSALISKSQEYCGRTENKIAHKIIADHLRAAAFLIAEGVLPGNEGRNYVLRRLIRRAARYIHLLGYNDSLLHRVFPVLID STSSAYMGDIYPELIRAKSLIETTLKSEEENFKDTLMKGISLLEKFTADLKSGDTLPGESAFKLYDTYGFPLDITLDILK EKKINFDQKGFDDAMGEQKERARAKWAGSGEKSVEQVWFDLIDKFGKTKFVGYEFNEVNDAKILAIVSSKNEVIDSAKEG EKITIILDKTPFYGESGGQVGDTGSLIKSDRSIIIVENTNKVNDLYLHRCIVKFGSICKGNTVAASIDKERRQTLRRNHS ATHLLHFVLRKILGDHVTQKGSLVAPDKLRFDFSHNTQVTQDQLFLVEDMVNSLIRENLSTSTKIQSMNQAIDEGAMALF GEKYGNQVRVVKIGDSRELCGGTHVEHTGEIGLFKIVTESSVAFGVRRIEALTGQEAINYVRDNEINLKKVAEFVKAPVS EIINRLNILNQERKESEAKIKNLYKKLISAESIKSTEINGINFVSHAFTNIPANIIREFVLQQQKPKTIIAFTATEKDKT VLIIKVSKDLANKISAKELVSIAVEKNCGGNAELAQTGCDSNKIDDAITAICSKIAASKHSTSYHHEPSYRRGANKQRDT
Sequences:
>Translated_880_residues MKLNEIRERFIKFFVNNDHEQVSSSPLIPEHDPTLMFTNAGMVQFKNIFTGAQKTEMKRAVSSQKCLRAGGKHNDLENVG YTTRHHTFFEMLGNFSFGDYFKETAIEFAWEFITKELSLDKNRLSITVYHTDDEAYEIWRKISGFSSDKIIRITTDDNFW SMGNTGPCGPCSEIFYDHGSPNLQEGDRIVEIWNLVFMEFNKDEEGNLHKLPKKCIDTGMGLERIAAVMQNVHDNYDIDL FSALISKSQEYCGRTENKIAHKIIADHLRAAAFLIAEGVLPGNEGRNYVLRRLIRRAARYIHLLGYNDSLLHRVFPVLID STSSAYMGDIYPELIRAKSLIETTLKSEEENFKDTLMKGISLLEKFTADLKSGDTLPGESAFKLYDTYGFPLDITLDILK EKKINFDQKGFDDAMGEQKERARAKWAGSGEKSVEQVWFDLIDKFGKTKFVGYEFNEVNDAKILAIVSSKNEVIDSAKEG EKITIILDKTPFYGESGGQVGDTGSLIKSDRSIIIVENTNKVNDLYLHRCIVKFGSICKGNTVAASIDKERRQTLRRNHS ATHLLHFVLRKILGDHVTQKGSLVAPDKLRFDFSHNTQVTQDQLFLVEDMVNSLIRENLSTSTKIQSMNQAIDEGAMALF GEKYGNQVRVVKIGDSRELCGGTHVEHTGEIGLFKIVTESSVAFGVRRIEALTGQEAINYVRDNEINLKKVAEFVKAPVS EIINRLNILNQERKESEAKIKNLYKKLISAESIKSTEINGINFVSHAFTNIPANIIREFVLQQQKPKTIIAFTATEKDKT VLIIKVSKDLANKISAKELVSIAVEKNCGGNAELAQTGCDSNKIDDAITAICSKIAASKHSTSYHHEPSYRRGANKQRDT >Mature_880_residues MKLNEIRERFIKFFVNNDHEQVSSSPLIPEHDPTLMFTNAGMVQFKNIFTGAQKTEMKRAVSSQKCLRAGGKHNDLENVG YTTRHHTFFEMLGNFSFGDYFKETAIEFAWEFITKELSLDKNRLSITVYHTDDEAYEIWRKISGFSSDKIIRITTDDNFW SMGNTGPCGPCSEIFYDHGSPNLQEGDRIVEIWNLVFMEFNKDEEGNLHKLPKKCIDTGMGLERIAAVMQNVHDNYDIDL FSALISKSQEYCGRTENKIAHKIIADHLRAAAFLIAEGVLPGNEGRNYVLRRLIRRAARYIHLLGYNDSLLHRVFPVLID STSSAYMGDIYPELIRAKSLIETTLKSEEENFKDTLMKGISLLEKFTADLKSGDTLPGESAFKLYDTYGFPLDITLDILK EKKINFDQKGFDDAMGEQKERARAKWAGSGEKSVEQVWFDLIDKFGKTKFVGYEFNEVNDAKILAIVSSKNEVIDSAKEG EKITIILDKTPFYGESGGQVGDTGSLIKSDRSIIIVENTNKVNDLYLHRCIVKFGSICKGNTVAASIDKERRQTLRRNHS ATHLLHFVLRKILGDHVTQKGSLVAPDKLRFDFSHNTQVTQDQLFLVEDMVNSLIRENLSTSTKIQSMNQAIDEGAMALF GEKYGNQVRVVKIGDSRELCGGTHVEHTGEIGLFKIVTESSVAFGVRRIEALTGQEAINYVRDNEINLKKVAEFVKAPVS EIINRLNILNQERKESEAKIKNLYKKLISAESIKSTEINGINFVSHAFTNIPANIIREFVLQQQKPKTIIAFTATEKDKT VLIIKVSKDLANKISAKELVSIAVEKNCGGNAELAQTGCDSNKIDDAITAICSKIAASKHSTSYHHEPSYRRGANKQRDT
Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin
COG id: COG0013
COG function: function code J; Alanyl-tRNA synthetase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]
Homologues:
Organism=Homo sapiens, GI109148542, Length=791, Percent_Identity=38.1795195954488, Blast_Score=506, Evalue=1e-143, Organism=Homo sapiens, GI38569417, Length=941, Percent_Identity=30.8182784272051, Blast_Score=430, Evalue=1e-120, Organism=Escherichia coli, GI1789048, Length=864, Percent_Identity=46.6435185185185, Blast_Score=771, Evalue=0.0, Organism=Caenorhabditis elegans, GI17506981, Length=970, Percent_Identity=34.639175257732, Blast_Score=484, Evalue=1e-137, Organism=Caenorhabditis elegans, GI17536681, Length=749, Percent_Identity=33.9118825100133, Blast_Score=381, Evalue=1e-105, Organism=Saccharomyces cerevisiae, GI6324911, Length=787, Percent_Identity=40.6607369758577, Blast_Score=546, Evalue=1e-156, Organism=Drosophila melanogaster, GI24582809, Length=981, Percent_Identity=34.2507645259939, Blast_Score=490, Evalue=1e-138, Organism=Drosophila melanogaster, GI45552267, Length=981, Percent_Identity=34.2507645259939, Blast_Score=490, Evalue=1e-138, Organism=Drosophila melanogaster, GI24658214, Length=806, Percent_Identity=30.893300248139, Blast_Score=360, Evalue=2e-99,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002318 - InterPro: IPR018162 - InterPro: IPR018165 - InterPro: IPR018164 - InterPro: IPR023033 - InterPro: IPR018163 - InterPro: IPR012947 [H]
Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]
EC number: =6.1.1.7 [H]
Molecular weight: Translated: 99197; Mature: 99197
Theoretical pI: Translated: 7.16; Mature: 7.16
Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKLNEIRERFIKFFVNNDHEQVSSSPLIPEHDPTLMFTNAGMVQFKNIFTGAQKTEMKRA CCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHH VSSQKCLRAGGKHNDLENVGYTTRHHTFFEMLGNFSFGDYFKETAIEFAWEFITKELSLD HHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC KNRLSITVYHTDDEAYEIWRKISGFSSDKIIRITTDDNFWSMGNTGPCGPCSEIFYDHGS CCEEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCCCCCHHHHHHCCCC PNLQEGDRIVEIWNLVFMEFNKDEEGNLHKLPKKCIDTGMGLERIAAVMQNVHDNYDIDL CCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHH FSALISKSQEYCGRTENKIAHKIIADHLRAAAFLIAEGVLPGNEGRNYVLRRLIRRAARY HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHE IHLLGYNDSLLHRVFPVLIDSTSSAYMGDIYPELIRAKSLIETTLKSEEENFKDTLMKGI EEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH SLLEKFTADLKSGDTLPGESAFKLYDTYGFPLDITLDILKEKKINFDQKGFDDAMGEQKE HHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHCCCCCCCCCCHHHCHHHH RARAKWAGSGEKSVEQVWFDLIDKFGKTKFVGYEFNEVNDAKILAIVSSKNEVIDSAKEG HHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHCCC EKITIILDKTPFYGESGGQVGDTGSLIKSDRSIIIVENTNKVNDLYLHRCIVKFGSICKG CEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCC NTVAASIDKERRQTLRRNHSATHLLHFVLRKILGDHVTQKGSLVAPDKLRFDFSHNTQVT CCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCCCCC QDQLFLVEDMVNSLIRENLSTSTKIQSMNQAIDEGAMALFGEKYGNQVRVVKIGDSRELC HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCC GGTHVEHTGEIGLFKIVTESSVAFGVRRIEALTGQEAINYVRDNEINLKKVAEFVKAPVS CCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH EIINRLNILNQERKESEAKIKNLYKKLISAESIKSTEINGINFVSHAFTNIPANIIREFV HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHH LQQQKPKTIIAFTATEKDKTVLIIKVSKDLANKISAKELVSIAVEKNCGGNAELAQTGCD HCCCCCCEEEEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC SNKIDDAITAICSKIAASKHSTSYHHEPSYRRGANKQRDT CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCC >Mature Secondary Structure MKLNEIRERFIKFFVNNDHEQVSSSPLIPEHDPTLMFTNAGMVQFKNIFTGAQKTEMKRA CCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHH VSSQKCLRAGGKHNDLENVGYTTRHHTFFEMLGNFSFGDYFKETAIEFAWEFITKELSLD HHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC KNRLSITVYHTDDEAYEIWRKISGFSSDKIIRITTDDNFWSMGNTGPCGPCSEIFYDHGS CCEEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCCCCCHHHHHHCCCC PNLQEGDRIVEIWNLVFMEFNKDEEGNLHKLPKKCIDTGMGLERIAAVMQNVHDNYDIDL CCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHH FSALISKSQEYCGRTENKIAHKIIADHLRAAAFLIAEGVLPGNEGRNYVLRRLIRRAARY HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHE IHLLGYNDSLLHRVFPVLIDSTSSAYMGDIYPELIRAKSLIETTLKSEEENFKDTLMKGI EEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH SLLEKFTADLKSGDTLPGESAFKLYDTYGFPLDITLDILKEKKINFDQKGFDDAMGEQKE HHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHCCCCCCCCCCHHHCHHHH RARAKWAGSGEKSVEQVWFDLIDKFGKTKFVGYEFNEVNDAKILAIVSSKNEVIDSAKEG HHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHCCC EKITIILDKTPFYGESGGQVGDTGSLIKSDRSIIIVENTNKVNDLYLHRCIVKFGSICKG CEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCC NTVAASIDKERRQTLRRNHSATHLLHFVLRKILGDHVTQKGSLVAPDKLRFDFSHNTQVT CCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCCCCC QDQLFLVEDMVNSLIRENLSTSTKIQSMNQAIDEGAMALFGEKYGNQVRVVKIGDSRELC HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCC GGTHVEHTGEIGLFKIVTESSVAFGVRRIEALTGQEAINYVRDNEINLKKVAEFVKAPVS CCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH EIINRLNILNQERKESEAKIKNLYKKLISAESIKSTEINGINFVSHAFTNIPANIIREFV HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHH LQQQKPKTIIAFTATEKDKTVLIIKVSKDLANKISAKELVSIAVEKNCGGNAELAQTGCD HCCCCCCEEEEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC SNKIDDAITAICSKIAASKHSTSYHHEPSYRRGANKQRDT CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA