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The map label for this gene is lysK [H]

Identifier: 225630564

GI number: 225630564

Start: 894840

End: 896375

Strand: Direct

Name: lysK [H]

Synonym: WRi_008250

Alternate gene names: 225630564

Gene position: 894840-896375 (Clockwise)

Preceding gene: 225630555

Following gene: 225630566

Centisome position: 61.89

GC content: 34.83

Gene sequence:

>1536_bases
ATGACAAGTTGGCCATTTCAAGAAGCAGAAAAAATATTGCAAGAGTTTCCTAATAAAAAAGAAATAATATTTGAAACTGG
TTATGGACCCTCTGGTTTACCCCACATAGGAACCTTTGGAGAGGTTTTCCGCACTACAGTTATTGTAAATGCATTAAAAA
AAATAGCTCCTGGTATAAAAACCAAAATCATTGCAATTTCAGATGATATGGATGGTTTGCGAAAAATACCAGATAATGTG
CCAAATCAAGAGATGCTAAGAGAGCACTTAAATAAACCATTGACTATGATACCTGATCCATTTGGCACTCATCAGAGTTA
TGGTCATCATATGAATTCATTGTTGTGTAAATTTCTTGATATGTTTGAATTTGAATACGAGTTCAGGAGTGCAACAGAGT
GTTATAAATCTGGCGTTTACGATGAAAAACTTTTACTCTTACTGAAAAATTATGATAAAGTGATGGACGTAATGCTTCCA
TCATTTCGGGAAGAAAGACAGCAGACTTATAGTCCATTCTTGCCAATATGCCCAAAAACTTCTCAAGTTTTACAAGTTCC
AGTAATTGAAACAAATACAGAGAAAGGAACAATCACATATGAAGACCCAAACGGGGGAAAAATAGAAGTTCCAGTGACAA
AAGGAAAATGTAAACTGCAATGGAAACCTGATTGGGGAATGAGGTGGGCCGCATTTGGAGTAAATTACGAAGCTCACGGA
AAAGATTTAACTCCATCTGCTGTGCTTTCAAGTCAAATATGTGAAATACTTGGAGAAAAGCCACCACTTCTGTTTTGCTA
CGAGCTTTTCCTTGATAAAGAAGGAAAGAAAATATCAAAATCAAAAGGAAATGGCATTTCAATTGAAGAGTGGCTAACTT
ACGCACCAACAGAGAGCTTAGCATTGTACATTTTTCAGAGTCCTAAAAAAGCTAAACGTTTATATTTTGATGTGATACCA
AAATCAACTGATGAATATTTAGAATTTGTAAAGCGTTATCATGAGAACAAAGAAAAGGATATAAATAATCCTGCTTGGCA
CATTCACCAGGGCAATGTTCCCAGTATAGAAACTTCAGGCATAAATTTCTCACTACTTTTAAACCTAGCAGCAGCTTGTA
ATGCTGAAAACAAAGAGATCCTATGGGGTTTTATATCCTCTTATGCACCAAACGTTACACCAGAAAATAATAAAATGCTT
GATAGACTTTCAGACTTTGCAGTAAAGTATTATCACGATTTTATCAAGCCAACAAAATCATATAAAACTCCAAATGAAAA
AGAAAAAGCAGCGTTACTAGATTTGAAGGACACTTTACATTCTCTATCTACAACTGCTACTGCTGAAGAAATTCAATCTC
AGGTATTTTCTATTGGAAAAAAATATGATTACACCAACTTACGTGACTGGTTTCAGCTATTATATGAAACGTTACTTGGC
CAAAAAACCGGTCCAAGAATGGGATCTTTCATAAAGCTTTACGGAATAGGTAATACGATTTCTCTCATAGAAAGCGCTAC
TAAATGTATACTTTAG

Upstream 100 bases:

>100_bases
CCTTCTAATAATAGCTCTAACACTAATACTATATCATTATATTAAAAAGCCATTGAAACTAGTAGCACTTTAAGTTAAGA
TACATTATTTAATCCCGATA

Downstream 100 bases:

>100_bases
AGTTTACCTCCTTTCATTTGAGCTCTTTAGTTTTAATAAGAATTCCTTGACAAACTCCGCTAGCCCCCTTATCATGAAAC
TGAAGCTATTGTATTTGCTT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MTSWPFQEAEKILQEFPNKKEIIFETGYGPSGLPHIGTFGEVFRTTVIVNALKKIAPGIKTKIIAISDDMDGLRKIPDNV
PNQEMLREHLNKPLTMIPDPFGTHQSYGHHMNSLLCKFLDMFEFEYEFRSATECYKSGVYDEKLLLLLKNYDKVMDVMLP
SFREERQQTYSPFLPICPKTSQVLQVPVIETNTEKGTITYEDPNGGKIEVPVTKGKCKLQWKPDWGMRWAAFGVNYEAHG
KDLTPSAVLSSQICEILGEKPPLLFCYELFLDKEGKKISKSKGNGISIEEWLTYAPTESLALYIFQSPKKAKRLYFDVIP
KSTDEYLEFVKRYHENKEKDINNPAWHIHQGNVPSIETSGINFSLLLNLAAACNAENKEILWGFISSYAPNVTPENNKML
DRLSDFAVKYYHDFIKPTKSYKTPNEKEKAALLDLKDTLHSLSTTATAEEIQSQVFSIGKKYDYTNLRDWFQLLYETLLG
QKTGPRMGSFIKLYGIGNTISLIESATKCIL

Sequences:

>Translated_511_residues
MTSWPFQEAEKILQEFPNKKEIIFETGYGPSGLPHIGTFGEVFRTTVIVNALKKIAPGIKTKIIAISDDMDGLRKIPDNV
PNQEMLREHLNKPLTMIPDPFGTHQSYGHHMNSLLCKFLDMFEFEYEFRSATECYKSGVYDEKLLLLLKNYDKVMDVMLP
SFREERQQTYSPFLPICPKTSQVLQVPVIETNTEKGTITYEDPNGGKIEVPVTKGKCKLQWKPDWGMRWAAFGVNYEAHG
KDLTPSAVLSSQICEILGEKPPLLFCYELFLDKEGKKISKSKGNGISIEEWLTYAPTESLALYIFQSPKKAKRLYFDVIP
KSTDEYLEFVKRYHENKEKDINNPAWHIHQGNVPSIETSGINFSLLLNLAAACNAENKEILWGFISSYAPNVTPENNKML
DRLSDFAVKYYHDFIKPTKSYKTPNEKEKAALLDLKDTLHSLSTTATAEEIQSQVFSIGKKYDYTNLRDWFQLLYETLLG
QKTGPRMGSFIKLYGIGNTISLIESATKCIL
>Mature_510_residues
TSWPFQEAEKILQEFPNKKEIIFETGYGPSGLPHIGTFGEVFRTTVIVNALKKIAPGIKTKIIAISDDMDGLRKIPDNVP
NQEMLREHLNKPLTMIPDPFGTHQSYGHHMNSLLCKFLDMFEFEYEFRSATECYKSGVYDEKLLLLLKNYDKVMDVMLPS
FREERQQTYSPFLPICPKTSQVLQVPVIETNTEKGTITYEDPNGGKIEVPVTKGKCKLQWKPDWGMRWAAFGVNYEAHGK
DLTPSAVLSSQICEILGEKPPLLFCYELFLDKEGKKISKSKGNGISIEEWLTYAPTESLALYIFQSPKKAKRLYFDVIPK
STDEYLEFVKRYHENKEKDINNPAWHIHQGNVPSIETSGINFSLLLNLAAACNAENKEILWGFISSYAPNVTPENNKMLD
RLSDFAVKYYHDFIKPTKSYKTPNEKEKAALLDLKDTLHSLSTTATAEEIQSQVFSIGKKYDYTNLRDWFQLLYETLLGQ
KTGPRMGSFIKLYGIGNTISLIESATKCIL

Specific function: Unknown

COG id: COG1384

COG function: function code J; Lysyl-tRNA synthetase (class I)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR002904
- InterPro:   IPR020754
- InterPro:   IPR020756
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01921 tRNA-synt_1f [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58295; Mature: 58163

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSWPFQEAEKILQEFPNKKEIIFETGYGPSGLPHIGTFGEVFRTTVIVNALKKIAPGIK
CCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
TKIIAISDDMDGLRKIPDNVPNQEMLREHLNKPLTMIPDPFGTHQSYGHHMNSLLCKFLD
EEEEEECCCCHHHHHCCCCCCCHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
MFEFEYEFRSATECYKSGVYDEKLLLLLKNYDKVMDVMLPSFREERQQTYSPFLPICPKT
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SQVLQVPVIETNTEKGTITYEDPNGGKIEVPVTKGKCKLQWKPDWGMRWAAFGVNYEAHG
CCEEEEEEEECCCCCCEEEEECCCCCEEEEEEECCEEEEEECCCCCCEEEEEECCEECCC
KDLTPSAVLSSQICEILGEKPPLLFCYELFLDKEGKKISKSKGNGISIEEWLTYAPTESL
CCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCHHCCCCCCCCHHHHHHCCCCCCE
ALYIFQSPKKAKRLYFDVIPKSTDEYLEFVKRYHENKEKDINNPAWHIHQGNVPSIETSG
EEEEECCCHHHHHHHHEECCCCHHHHHHHHHHHHCCHHCCCCCCCEEEECCCCCCEEECC
INFSLLLNLAAACNAENKEILWGFISSYAPNVTPENNKMLDRLSDFAVKYYHDFIKPTKS
CCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHC
YKTPNEKEKAALLDLKDTLHSLSTTATAEEIQSQVFSIGKKYDYTNLRDWFQLLYETLLG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
QKTGPRMGSFIKLYGIGNTISLIESATKCIL
CCCCCCCCCEEEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TSWPFQEAEKILQEFPNKKEIIFETGYGPSGLPHIGTFGEVFRTTVIVNALKKIAPGIK
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
TKIIAISDDMDGLRKIPDNVPNQEMLREHLNKPLTMIPDPFGTHQSYGHHMNSLLCKFLD
EEEEEECCCCHHHHHCCCCCCCHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
MFEFEYEFRSATECYKSGVYDEKLLLLLKNYDKVMDVMLPSFREERQQTYSPFLPICPKT
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SQVLQVPVIETNTEKGTITYEDPNGGKIEVPVTKGKCKLQWKPDWGMRWAAFGVNYEAHG
CCEEEEEEEECCCCCCEEEEECCCCCEEEEEEECCEEEEEECCCCCCEEEEEECCEECCC
KDLTPSAVLSSQICEILGEKPPLLFCYELFLDKEGKKISKSKGNGISIEEWLTYAPTESL
CCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCHHCCCCCCCCHHHHHHCCCCCCE
ALYIFQSPKKAKRLYFDVIPKSTDEYLEFVKRYHENKEKDINNPAWHIHQGNVPSIETSG
EEEEECCCHHHHHHHHEECCCCHHHHHHHHHHHHCCHHCCCCCCCEEEECCCCCCEEECC
INFSLLLNLAAACNAENKEILWGFISSYAPNVTPENNKMLDRLSDFAVKYYHDFIKPTKS
CCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHC
YKTPNEKEKAALLDLKDTLHSLSTTATAEEIQSQVFSIGKKYDYTNLRDWFQLLYETLLG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
QKTGPRMGSFIKLYGIGNTISLIESATKCIL
CCCCCCCCCEEEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA