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The map label for this gene is recG [H]

Identifier: 225630537

GI number: 225630537

Start: 849882

End: 851903

Strand: Direct

Name: recG [H]

Synonym: WRi_007900

Alternate gene names: 225630537

Gene position: 849882-851903 (Clockwise)

Preceding gene: 225630535

Following gene: 225630538

Centisome position: 58.78

GC content: 36.1

Gene sequence:

>2022_bases
ATGAGCAATCAGATTTTTCTAAATAGCAGGCTGGAGAATATACCTAAATTTCATTCCACAATATTACCTAAACTTTGTGG
TGGAGATAGAGTAATGGACTTGCTATTTTATAGGCCACTTAGTTATGTGGATAGAAGTAAGTCACTGCCTGATGCTCAAG
TTGGAGAATTTATAACTTTTATTGCAAAAGTTTATGAGCATCAGTCGCCCACTTTTAGAGGTAGACCATATAAAATAGTT
GTTGAAAGTGAGAGTCAGTATATATTCATAGTCTTTTTTAATTACTCAGTTAAGTATTTATATAAGTTATTTCCAATTGG
AGCTTCTGTAATCATCAGTGGTAAACTCGAAAAATTTGCTGAACATTGGCAAATTACTCATCCAGATTACGTGTCGCTTG
ACATCAACCAATTTAAAAAAATAGCTTGCATAGAGCCAGTTTACCAATTATGCCGTGGCATTACCAATAAGCGCATTGGG
AACATAATAAGCTCTAATCTAAAGGAATTGCCTGATTTACCAGAGTGGATAGATGATACATTAATCAAGCAAAAAAAATG
GTTAAGTTGGAGGGAAAGCATCATAAAGTTGCACAGACCAAGCTCATTGGCGGAAGCTGAGGTTTGTAGAAAAAGGCTTG
CTTATGATGAATTGTTTGCGTATCAGCTAGCGCTGAAACTTGCAAGAAAAAATCATGTGAAAGAAAGGGGAAGAGAATTT
ATAATATTGAGTAAATACAAAGAGCAAGTTTTAAATGAATTGTCGTTTCAATTAACAAATGATCAAACTCGTGCAATAGA
TGAGATCTCAGAGAGACAAAAATCCAAGTATCGCATGATAAGCTTGCTGCAAGGTGATGTTGGTAGTGGCAAAACCGTAG
TTGCACTTTTTGCGATGATAAATGTGGTAGAAAACAACATGCAGGCAGCTCTAATGGCACCGACTACTATCTTGGCGGAG
CAACATTATAATTGGATTGAGGAAGTTTTATCCTGTACCGATATAAAAGTTGCGCTGCTTACTGGTAAAACTTCGCGCAA
GGAAAGAAAGATTATCATGAACGAACTTGCAAGTGGTGTTTTAAATATAGTGATTGGCACTCATGCACTATTTCAAGCTA
ACGTTACATTTAAAAATTTAGGGCTTGCAGTCATAGACGAGCAACAGCGATTTGGAGTGATGCAGAGAAATCGCTTGGTA
GGAAAAGGAGAAAATGCCGATATACTTTTCGTTACTGCAACTCCCATTCCAAGAACCTTGCAGCAAGCTATGTATGGTGA
TGTTGAATGTTCAATTTTGAAAGAAAAACCAAAATCCAGATTGCCAATAAAAACTGTAACTATGAACATTAGTAGGGTGC
CAGATGTTATTGAAAAGCTAAAAGGTGCTATAAACAGGGGCGAAAAAGCATATTGGATTTGTCCATATATAGAAGAAAAC
GAAGAGACCAATATTGCTGCAGCAGAGATGCGTTTTCAGGAATTACAAAAAACATTTCTTGATAAAGTCGGGATAATACA
CGGAAAATTGACTCAGGAGCAGAAAGATCAAGTTATGTTTTCCTTCAAAAGGAATGAATTTTCTCTGCTTGTTGCAACCA
CTGTGATAGAAGTTGGTATAGATGTGCCAGATGCAACCATCATGATTATAGAAAATGCAGAGCAATTTGGGTTATCACAA
TTGCATCAGTTAAGAGGCAGAGTAGGGCGAGGAAACAAGCCATCTTTTTGTGTATTATTATACGATAATTTAAGTAAAAG
CTCATCTTCAAAGTTAAAGATCATGTGTGAGTCACAAGATGGATTTTACATTGCTGAAAGGGATATGATGCTAAGAGGCA
GTGGAGATATTTTAGGCACAAAACAATCAGGATGTATGGAATTTAAATTTGCTGATTTATATCAGGACAAAGAGCTGCTC
AATCTCGCGTATAATAACGCAAAAGGCGCAATGGCTGATTTTGAATTACTGCTTGATATATTTGAATATAGGAGTAGATT
ACATTTTTCAAAATTTCAGTAG

Upstream 100 bases:

>100_bases
TATGACGTTAGAGAGCTGTGCTAGCTATAGTGCATACCTGACCATAAGGTGACAAGATTTTAAAAAGGCTCTATAACAGT
TTACACATGTATGAATCTCA

Downstream 100 bases:

>100_bases
GAAAACCAGGTAGCCCGCAGAAACGAAAAAACTTCCTTGACAAACCTCACCAGCCCCCTTATCATGAAACTGAAGCTATT
GTATTTGCTTTCGCCAATCT

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MSNQIFLNSRLENIPKFHSTILPKLCGGDRVMDLLFYRPLSYVDRSKSLPDAQVGEFITFIAKVYEHQSPTFRGRPYKIV
VESESQYIFIVFFNYSVKYLYKLFPIGASVIISGKLEKFAEHWQITHPDYVSLDINQFKKIACIEPVYQLCRGITNKRIG
NIISSNLKELPDLPEWIDDTLIKQKKWLSWRESIIKLHRPSSLAEAEVCRKRLAYDELFAYQLALKLARKNHVKERGREF
IILSKYKEQVLNELSFQLTNDQTRAIDEISERQKSKYRMISLLQGDVGSGKTVVALFAMINVVENNMQAALMAPTTILAE
QHYNWIEEVLSCTDIKVALLTGKTSRKERKIIMNELASGVLNIVIGTHALFQANVTFKNLGLAVIDEQQRFGVMQRNRLV
GKGENADILFVTATPIPRTLQQAMYGDVECSILKEKPKSRLPIKTVTMNISRVPDVIEKLKGAINRGEKAYWICPYIEEN
EETNIAAAEMRFQELQKTFLDKVGIIHGKLTQEQKDQVMFSFKRNEFSLLVATTVIEVGIDVPDATIMIIENAEQFGLSQ
LHQLRGRVGRGNKPSFCVLLYDNLSKSSSSKLKIMCESQDGFYIAERDMMLRGSGDILGTKQSGCMEFKFADLYQDKELL
NLAYNNAKGAMADFELLLDIFEYRSRLHFSKFQ

Sequences:

>Translated_673_residues
MSNQIFLNSRLENIPKFHSTILPKLCGGDRVMDLLFYRPLSYVDRSKSLPDAQVGEFITFIAKVYEHQSPTFRGRPYKIV
VESESQYIFIVFFNYSVKYLYKLFPIGASVIISGKLEKFAEHWQITHPDYVSLDINQFKKIACIEPVYQLCRGITNKRIG
NIISSNLKELPDLPEWIDDTLIKQKKWLSWRESIIKLHRPSSLAEAEVCRKRLAYDELFAYQLALKLARKNHVKERGREF
IILSKYKEQVLNELSFQLTNDQTRAIDEISERQKSKYRMISLLQGDVGSGKTVVALFAMINVVENNMQAALMAPTTILAE
QHYNWIEEVLSCTDIKVALLTGKTSRKERKIIMNELASGVLNIVIGTHALFQANVTFKNLGLAVIDEQQRFGVMQRNRLV
GKGENADILFVTATPIPRTLQQAMYGDVECSILKEKPKSRLPIKTVTMNISRVPDVIEKLKGAINRGEKAYWICPYIEEN
EETNIAAAEMRFQELQKTFLDKVGIIHGKLTQEQKDQVMFSFKRNEFSLLVATTVIEVGIDVPDATIMIIENAEQFGLSQ
LHQLRGRVGRGNKPSFCVLLYDNLSKSSSSKLKIMCESQDGFYIAERDMMLRGSGDILGTKQSGCMEFKFADLYQDKELL
NLAYNNAKGAMADFELLLDIFEYRSRLHFSKFQ
>Mature_672_residues
SNQIFLNSRLENIPKFHSTILPKLCGGDRVMDLLFYRPLSYVDRSKSLPDAQVGEFITFIAKVYEHQSPTFRGRPYKIVV
ESESQYIFIVFFNYSVKYLYKLFPIGASVIISGKLEKFAEHWQITHPDYVSLDINQFKKIACIEPVYQLCRGITNKRIGN
IISSNLKELPDLPEWIDDTLIKQKKWLSWRESIIKLHRPSSLAEAEVCRKRLAYDELFAYQLALKLARKNHVKERGREFI
ILSKYKEQVLNELSFQLTNDQTRAIDEISERQKSKYRMISLLQGDVGSGKTVVALFAMINVVENNMQAALMAPTTILAEQ
HYNWIEEVLSCTDIKVALLTGKTSRKERKIIMNELASGVLNIVIGTHALFQANVTFKNLGLAVIDEQQRFGVMQRNRLVG
KGENADILFVTATPIPRTLQQAMYGDVECSILKEKPKSRLPIKTVTMNISRVPDVIEKLKGAINRGEKAYWICPYIEENE
ETNIAAAEMRFQELQKTFLDKVGIIHGKLTQEQKDQVMFSFKRNEFSLLVATTVIEVGIDVPDATIMIIENAEQFGLSQL
HQLRGRVGRGNKPSFCVLLYDNLSKSSSSKLKIMCESQDGFYIAERDMMLRGSGDILGTKQSGCMEFKFADLYQDKELLN
LAYNNAKGAMADFELLLDIFEYRSRLHFSKFQ

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=632, Percent_Identity=37.6582278481013, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1787357, Length=405, Percent_Identity=38.0246913580247, Blast_Score=265, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77076; Mature: 76945

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQIFLNSRLENIPKFHSTILPKLCGGDRVMDLLFYRPLSYVDRSKSLPDAQVGEFITF
CCCCEEECCHHHHCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHH
IAKVYEHQSPTFRGRPYKIVVESESQYIFIVFFNYSVKYLYKLFPIGASVIISGKLEKFA
HHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCHHHHHHHHHCCCCEEEEECHHHHHH
EHWQITHPDYVSLDINQFKKIACIEPVYQLCRGITNKRIGNIISSNLKELPDLPEWIDDT
HHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHH
LIKQKKWLSWRESIIKLHRPSSLAEAEVCRKRLAYDELFAYQLALKLARKNHVKERGREF
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IILSKYKEQVLNELSFQLTNDQTRAIDEISERQKSKYRMISLLQGDVGSGKTVVALFAMI
EEEHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NVVENNMQAALMAPTTILAEQHYNWIEEVLSCTDIKVALLTGKTSRKERKIIMNELASGV
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHH
LNIVIGTHALFQANVTFKNLGLAVIDEQQRFGVMQRNRLVGKGENADILFVTATPIPRTL
HHHHHHHHHHEEECCCHHHCCEEEECCHHHHCHHHHCCCCCCCCCCCEEEEECCCCHHHH
QQAMYGDVECSILKEKPKSRLPIKTVTMNISRVPDVIEKLKGAINRGEKAYWICPYIEEN
HHHHCCCCEEEEECCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
EETNIAAAEMRFQELQKTFLDKVGIIHGKLTQEQKDQVMFSFKRNEFSLLVATTVIEVGI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEEECCCCEEEEHHHHHHHHCC
DVPDATIMIIENAEQFGLSQLHQLRGRVGRGNKPSFCVLLYDNLSKSSSSKLKIMCESQD
CCCCCEEEEEECCHHHCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCC
GFYIAERDMMLRGSGDILGTKQSGCMEFKFADLYQDKELLNLAYNNAKGAMADFELLLDI
CEEEEECCEEEECCCCEEECCCCCCEEEEHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHH
FEYRSRLHFSKFQ
HHHHHHCCCCCCC
>Mature Secondary Structure 
SNQIFLNSRLENIPKFHSTILPKLCGGDRVMDLLFYRPLSYVDRSKSLPDAQVGEFITF
CCCEEECCHHHHCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHH
IAKVYEHQSPTFRGRPYKIVVESESQYIFIVFFNYSVKYLYKLFPIGASVIISGKLEKFA
HHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCHHHHHHHHHCCCCEEEEECHHHHHH
EHWQITHPDYVSLDINQFKKIACIEPVYQLCRGITNKRIGNIISSNLKELPDLPEWIDDT
HHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHH
LIKQKKWLSWRESIIKLHRPSSLAEAEVCRKRLAYDELFAYQLALKLARKNHVKERGREF
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IILSKYKEQVLNELSFQLTNDQTRAIDEISERQKSKYRMISLLQGDVGSGKTVVALFAMI
EEEHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NVVENNMQAALMAPTTILAEQHYNWIEEVLSCTDIKVALLTGKTSRKERKIIMNELASGV
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHH
LNIVIGTHALFQANVTFKNLGLAVIDEQQRFGVMQRNRLVGKGENADILFVTATPIPRTL
HHHHHHHHHHEEECCCHHHCCEEEECCHHHHCHHHHCCCCCCCCCCCEEEEECCCCHHHH
QQAMYGDVECSILKEKPKSRLPIKTVTMNISRVPDVIEKLKGAINRGEKAYWICPYIEEN
HHHHCCCCEEEEECCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
EETNIAAAEMRFQELQKTFLDKVGIIHGKLTQEQKDQVMFSFKRNEFSLLVATTVIEVGI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEEECCCCEEEEHHHHHHHHCC
DVPDATIMIIENAEQFGLSQLHQLRGRVGRGNKPSFCVLLYDNLSKSSSSKLKIMCESQD
CCCCCEEEEEECCHHHCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCC
GFYIAERDMMLRGSGDILGTKQSGCMEFKFADLYQDKELLNLAYNNAKGAMADFELLLDI
CEEEEECCEEEECCCCEEECCCCCCEEEEHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHH
FEYRSRLHFSKFQ
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]