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The map label for this gene is hyfD [H]

Identifier: 225630529

GI number: 225630529

Start: 842576

End: 844072

Strand: Direct

Name: hyfD [H]

Synonym: WRi_007820

Alternate gene names: 225630529

Gene position: 842576-844072 (Clockwise)

Preceding gene: 225630528

Following gene: 225630535

Centisome position: 58.27

GC content: 34.4

Gene sequence:

>1497_bases
ATGGACTGTGTTTTAACGTCTCTTTCCTTACCATTTTCAATTACCGAGAATTATTTATTAATTACTGCGTTGATTCCACT
TTTAAGTGCGTTAGTTATTTTTTTTACTGGTAAATGGCCCAGAGTGAACAATAGTATTACTGTCACTTCTTCTGTACTTC
TGTTTACGTATACGTGTCTGTGCACTTTATATTGGGTATATGGTGATCATTCTCAATTTATTCTCATGGATTTTGGTAAT
AATTTGCACATTAGTTTAAAGCTAGAGTCTACCGGAGTAATATTCAGTTTATTAATATCTTTTTTATGGATACTAACCAG
TATATATGCAATATGTTATATGCAGAATAACTATGCTGATAGCAATCACTCAAGTTTTCTATGCTTTTTTTCAATATCGA
TTAGCTGTGCAATGTTTATTGCTTTTTCTGGAGACTTGCTCACTACATTTGTCTTTTATGAGCTTCTAACTATTAGTACA
TACCCTCTGGTTACTTACAACTCAACAAATGAATCAATGATTGCAGGACGCTATTACTTTGGCGTGCTATTTTTCTCTTC
TTTGGTATTGTTTTTTCCTGCAATAGGCCTTTTATATAATGAATTTCATACTTTAGACTTTGTAAGCGGTGGAATATTCA
AATTTGATACTTCACTCATCACTTTCATTGCAGTGTGTTTTGCTATGCTGATTTACGGAATAGGAAAAGCTGCATTAATG
CCAATGCATTTTTGGTTACCAAAAGCAATGGTTGCACCAACTCCTGTGAGTGCACTACTGCACGCTGTTGCTGTTGTGAA
ATCCGGAGTTTTCATCATCATAAAAGTTATATTATACACTTTCGGCGTCGATAACATGCAACGTTTTGTGCAGCAAAATT
GGTTCACTGGAGGGTGGCTTCCATACGTTGCTGGATTTACTATTATTACTGCATCATTGATTGCATTAAAGCAAAAAGAA
TTAAAGAAATTGCTTGCTTACTCTACCATCTCTCAATTGTCGTATATTATATTATTTGCTTCAATTTTTAGTGAGCTCAG
CATGAGGGTTGCAATTTTTCAATTAGTTTGCCACGCATTTGCAAAAATCACTTTGTTTTTCATTGCCGGAGCAATAATAA
CGAGAGCAGGTGAGAAATATATAGATAAGATTTACGGTATAGGACGCAGTATGCCAATTAGCATGACAGCATTTGCTATA
GGTGCATTGTCTATGATAGGGGTGCCGCCTGCTCCGACTTTTTGGGGTAAATTTCTCATTTTTCAAGCTGTTTTCAACTC
TGGCAATGTAATACTTTCTGTGTTTGTAACTTTAGTGTTAATTGTTAGTACTATACTCAACGCTCTGTACTTTTTGCCGA
TAATCTACAACGCCTTTTTCTTAAAACCTTCTCAGAATTACTTTATTAAAAAAACGCCAATTCTTCTAGTTTTACCTCCA
GTTATTACTGCTATGTGCACTTTGGTTATTTTTTTTAGCTACCAAGTAATATTTTAA

Upstream 100 bases:

>100_bases
AGTATAGCTATAGCATATGCAACAACTAAAAAAACATAGCGGTTTTTCTATGATTTTAGTATAATTCTAGCTTTACCTAA
ACAAAAAGATGAATTATTTA

Downstream 100 bases:

>100_bases
AATCTGTATTTATAACCATTAACAATAAATTTAAATGGTATTATACCAGCCAGAGTTGCTTTTATATTCGACAAAGTGAA
GCTAATCTCCACAGTATCCT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MDCVLTSLSLPFSITENYLLITALIPLLSALVIFFTGKWPRVNNSITVTSSVLLFTYTCLCTLYWVYGDHSQFILMDFGN
NLHISLKLESTGVIFSLLISFLWILTSIYAICYMQNNYADSNHSSFLCFFSISISCAMFIAFSGDLLTTFVFYELLTIST
YPLVTYNSTNESMIAGRYYFGVLFFSSLVLFFPAIGLLYNEFHTLDFVSGGIFKFDTSLITFIAVCFAMLIYGIGKAALM
PMHFWLPKAMVAPTPVSALLHAVAVVKSGVFIIIKVILYTFGVDNMQRFVQQNWFTGGWLPYVAGFTIITASLIALKQKE
LKKLLAYSTISQLSYIILFASIFSELSMRVAIFQLVCHAFAKITLFFIAGAIITRAGEKYIDKIYGIGRSMPISMTAFAI
GALSMIGVPPAPTFWGKFLIFQAVFNSGNVILSVFVTLVLIVSTILNALYFLPIIYNAFFLKPSQNYFIKKTPILLVLPP
VITAMCTLVIFFSYQVIF

Sequences:

>Translated_498_residues
MDCVLTSLSLPFSITENYLLITALIPLLSALVIFFTGKWPRVNNSITVTSSVLLFTYTCLCTLYWVYGDHSQFILMDFGN
NLHISLKLESTGVIFSLLISFLWILTSIYAICYMQNNYADSNHSSFLCFFSISISCAMFIAFSGDLLTTFVFYELLTIST
YPLVTYNSTNESMIAGRYYFGVLFFSSLVLFFPAIGLLYNEFHTLDFVSGGIFKFDTSLITFIAVCFAMLIYGIGKAALM
PMHFWLPKAMVAPTPVSALLHAVAVVKSGVFIIIKVILYTFGVDNMQRFVQQNWFTGGWLPYVAGFTIITASLIALKQKE
LKKLLAYSTISQLSYIILFASIFSELSMRVAIFQLVCHAFAKITLFFIAGAIITRAGEKYIDKIYGIGRSMPISMTAFAI
GALSMIGVPPAPTFWGKFLIFQAVFNSGNVILSVFVTLVLIVSTILNALYFLPIIYNAFFLKPSQNYFIKKTPILLVLPP
VITAMCTLVIFFSYQVIF
>Mature_498_residues
MDCVLTSLSLPFSITENYLLITALIPLLSALVIFFTGKWPRVNNSITVTSSVLLFTYTCLCTLYWVYGDHSQFILMDFGN
NLHISLKLESTGVIFSLLISFLWILTSIYAICYMQNNYADSNHSSFLCFFSISISCAMFIAFSGDLLTTFVFYELLTIST
YPLVTYNSTNESMIAGRYYFGVLFFSSLVLFFPAIGLLYNEFHTLDFVSGGIFKFDTSLITFIAVCFAMLIYGIGKAALM
PMHFWLPKAMVAPTPVSALLHAVAVVKSGVFIIIKVILYTFGVDNMQRFVQQNWFTGGWLPYVAGFTIITASLIALKQKE
LKKLLAYSTISQLSYIILFASIFSELSMRVAIFQLVCHAFAKITLFFIAGAIITRAGEKYIDKIYGIGRSMPISMTAFAI
GALSMIGVPPAPTFWGKFLIFQAVFNSGNVILSVFVTLVLIVSTILNALYFLPIIYNAFFLKPSQNYFIKKTPILLVLPP
VITAMCTLVIFFSYQVIF

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=188, Percent_Identity=32.4468085106383, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1788829, Length=430, Percent_Identity=28.6046511627907, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1788614, Length=465, Percent_Identity=27.0967741935484, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788827, Length=360, Percent_Identity=28.0555555555556, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1788831, Length=357, Percent_Identity=26.890756302521, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693160, Length=413, Percent_Identity=24.9394673123487, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI2367154, Length=346, Percent_Identity=23.9884393063584, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 55725; Mature: 55725

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCVLTSLSLPFSITENYLLITALIPLLSALVIFFTGKWPRVNNSITVTSSVLLFTYTCL
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHH
CTLYWVYGDHSQFILMDFGNNLHISLKLESTGVIFSLLISFLWILTSIYAICYMQNNYAD
HHHHHHCCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEECCCCC
SNHSSFLCFFSISISCAMFIAFSGDLLTTFVFYELLTISTYPLVTYNSTNESMIAGRYYF
CCCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEHHHHHH
GVLFFSSLVLFFPAIGLLYNEFHTLDFVSGGIFKFDTSLITFIAVCFAMLIYGIGKAALM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHH
PMHFWLPKAMVAPTPVSALLHAVAVVKSGVFIIIKVILYTFGVDNMQRFVQQNWFTGGWL
HHHHHCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCH
PYVAGFTIITASLIALKQKELKKLLAYSTISQLSYIILFASIFSELSMRVAIFQLVCHAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKITLFFIAGAIITRAGEKYIDKIYGIGRSMPISMTAFAIGALSMIGVPPAPTFWGKFLI
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
FQAVFNSGNVILSVFVTLVLIVSTILNALYFLPIIYNAFFLKPSQNYFIKKTPILLVLPP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEECCCEEEEHHH
VITAMCTLVIFFSYQVIF
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDCVLTSLSLPFSITENYLLITALIPLLSALVIFFTGKWPRVNNSITVTSSVLLFTYTCL
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHH
CTLYWVYGDHSQFILMDFGNNLHISLKLESTGVIFSLLISFLWILTSIYAICYMQNNYAD
HHHHHHCCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEECCCCC
SNHSSFLCFFSISISCAMFIAFSGDLLTTFVFYELLTISTYPLVTYNSTNESMIAGRYYF
CCCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEHHHHHH
GVLFFSSLVLFFPAIGLLYNEFHTLDFVSGGIFKFDTSLITFIAVCFAMLIYGIGKAALM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHH
PMHFWLPKAMVAPTPVSALLHAVAVVKSGVFIIIKVILYTFGVDNMQRFVQQNWFTGGWL
HHHHHCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCH
PYVAGFTIITASLIALKQKELKKLLAYSTISQLSYIILFASIFSELSMRVAIFQLVCHAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKITLFFIAGAIITRAGEKYIDKIYGIGRSMPISMTAFAIGALSMIGVPPAPTFWGKFLI
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
FQAVFNSGNVILSVFVTLVLIVSTILNALYFLPIIYNAFFLKPSQNYFIKKTPILLVLPP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEECCCEEEEHHH
VITAMCTLVIFFSYQVIF
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]