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The map label for this gene is lpd3 [H]

Identifier: 225630478

GI number: 225630478

Start: 785359

End: 786729

Strand: Reverse

Name: lpd3 [H]

Synonym: WRi_007260

Alternate gene names: 225630478

Gene position: 786729-785359 (Counterclockwise)

Preceding gene: 225630479

Following gene: 225630477

Centisome position: 54.41

GC content: 39.24

Gene sequence:

>1371_bases
ATGACTGATTATGATTTAATTGTTATAGGTGGTGGCCCAGGAGGCTATAAGTGCGCTATCGCTGCTGCAAAGCTTGGATT
GAAAGTTGCCTGTATAGATAAAAATAGCATTTTTGGTGGCACATGCCTCAGAGTTGGGTGCATACCCTCCAAAGCATTGC
TCCATTCTTCCTATCAGTATGCTCACACGAAAAATGATCTGTCGAAGCTTGGCATAAAAATTAAGGACGCAAGTTTCGAT
TTAAAAGAAATGCTAGGTTATAAGGACGCCAGAGTTCAGGAACTTGGAAAAGGTATAGAATATCTGTTTAACCTTCACAA
AATCACTAAAATCAATGGGCTTGCTTCTTTTGACCAAGGTAATCTTGAAGTTTCAGTTGAAGGTAAGGTGCTGAAGACAA
AAAATATAGTAATTGCAACCGGTTCTGACGTTATTTCTTTGCCAGGAATTAATATCGATGAGAAAAATATTATTTCATCA
ACTGGTGCATTATCTTTAACTGAAGTACCAAAAAAACTTGTCGTAATCGGAGCCGGGGCAATAGGGCTTGAAATGTCTTC
TGTATGGAGCAGGCTAGGGTCTGAAGTCACTGTAGTAGAATTTTTTGATAGAATCGCTGCAGCAATGGATGGAGAATTAA
GTAAGTCTCTACTTTCTAGTCTACAAAAACAAGGAATAAAATTTTTACTCAGTACTAAAGTTGAGGAGATAAAACAAAGT
AGTAATTCTTTGAGTGTGAAAGTTTGCTCTGTAAAAGATAATCAAACAAACACTATAGAGGCAGATAAGGTGCTGGTTGC
AGTAGGTCGCAAACCATGCACTGAGAGTCTTGAAAAAATAGAGAAAGACAGTCGTGGTTTCGTTCAAGTTAACAACAGAT
ATGAAACTAATGTAAAAGGAATATTTGCTATTGGTGATGTGATCGGTGGAGCAATGCTTGCTCATAAGGCAGAAGAAGAA
GGAGTGGCAGTTGCAGAGATAATCGCTGGGCAAGTACCTCACGTTGATTATGAAATCATACCATCTGTCATTTACACTCA
CCCTGCGGTTTCTTCAATCGGTAAAACTGAAGAGGAGTTGAAAAGTGTTGGCCGTAAGTACAAAGTTGGTAAATGTCAAT
TTGCTGCAAACGGCAGAGCAAAAATCACTGATGATGCTGAAGGATTCGTGAAAGTGCTGACTTGTAGCAGAGCAGATACA
ATACTAGGTGTGCATATCATAGGAGCATACGCTGACACGCTAATAAACGAAGCAGCGGTTGCAATGGCATATGGCGCAGC
AGCAGAGGATATATACAGAATTTGTCACTCTCATCCTGATATAAATGAAGCCTTTCGAGATGCGTGCATCGATGCTTTCT
TTAAAAAATAA

Upstream 100 bases:

>100_bases
CCTGGTAATGTGTTTCACACTCCAGTGTTTACTCGCAATAAGATATATGTAACAACTGAGAAGAATGGTGTTTATTCTTT
AGAAAATAGGTTTGTTTTTT

Downstream 100 bases:

>100_bases
TTGTGGACCTCGGTTCAATCATTGAAAAATGGTATGAGTGGCTGAGGTGCAACAGATCTTATTCACCAAATACTTTAGAG
TCATACATGAGGGACTTGAA

Product: Dihydrolipoamide dehydrogenase E3 component

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTDYDLIVIGGGPGGYKCAIAAAKLGLKVACIDKNSIFGGTCLRVGCIPSKALLHSSYQYAHTKNDLSKLGIKIKDASFD
LKEMLGYKDARVQELGKGIEYLFNLHKITKINGLASFDQGNLEVSVEGKVLKTKNIVIATGSDVISLPGINIDEKNIISS
TGALSLTEVPKKLVVIGAGAIGLEMSSVWSRLGSEVTVVEFFDRIAAAMDGELSKSLLSSLQKQGIKFLLSTKVEEIKQS
SNSLSVKVCSVKDNQTNTIEADKVLVAVGRKPCTESLEKIEKDSRGFVQVNNRYETNVKGIFAIGDVIGGAMLAHKAEEE
GVAVAEIIAGQVPHVDYEIIPSVIYTHPAVSSIGKTEEELKSVGRKYKVGKCQFAANGRAKITDDAEGFVKVLTCSRADT
ILGVHIIGAYADTLINEAAVAMAYGAAAEDIYRICHSHPDINEAFRDACIDAFFKK

Sequences:

>Translated_456_residues
MTDYDLIVIGGGPGGYKCAIAAAKLGLKVACIDKNSIFGGTCLRVGCIPSKALLHSSYQYAHTKNDLSKLGIKIKDASFD
LKEMLGYKDARVQELGKGIEYLFNLHKITKINGLASFDQGNLEVSVEGKVLKTKNIVIATGSDVISLPGINIDEKNIISS
TGALSLTEVPKKLVVIGAGAIGLEMSSVWSRLGSEVTVVEFFDRIAAAMDGELSKSLLSSLQKQGIKFLLSTKVEEIKQS
SNSLSVKVCSVKDNQTNTIEADKVLVAVGRKPCTESLEKIEKDSRGFVQVNNRYETNVKGIFAIGDVIGGAMLAHKAEEE
GVAVAEIIAGQVPHVDYEIIPSVIYTHPAVSSIGKTEEELKSVGRKYKVGKCQFAANGRAKITDDAEGFVKVLTCSRADT
ILGVHIIGAYADTLINEAAVAMAYGAAAEDIYRICHSHPDINEAFRDACIDAFFKK
>Mature_455_residues
TDYDLIVIGGGPGGYKCAIAAAKLGLKVACIDKNSIFGGTCLRVGCIPSKALLHSSYQYAHTKNDLSKLGIKIKDASFDL
KEMLGYKDARVQELGKGIEYLFNLHKITKINGLASFDQGNLEVSVEGKVLKTKNIVIATGSDVISLPGINIDEKNIISST
GALSLTEVPKKLVVIGAGAIGLEMSSVWSRLGSEVTVVEFFDRIAAAMDGELSKSLLSSLQKQGIKFLLSTKVEEIKQSS
NSLSVKVCSVKDNQTNTIEADKVLVAVGRKPCTESLEKIEKDSRGFVQVNNRYETNVKGIFAIGDVIGGAMLAHKAEEEG
VAVAEIIAGQVPHVDYEIIPSVIYTHPAVSSIGKTEEELKSVGRKYKVGKCQFAANGRAKITDDAEGFVKVLTCSRADTI
LGVHIIGAYADTLINEAAVAMAYGAAAEDIYRICHSHPDINEAFRDACIDAFFKK

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=466, Percent_Identity=48.9270386266094, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI50301238, Length=462, Percent_Identity=29.4372294372294, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI22035672, Length=480, Percent_Identity=28.3333333333333, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI291045266, Length=477, Percent_Identity=28.0922431865828, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519430, Length=474, Percent_Identity=27.2151898734177, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI33519428, Length=474, Percent_Identity=27.2151898734177, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI33519426, Length=474, Percent_Identity=27.2151898734177, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI148277065, Length=474, Percent_Identity=27.2151898734177, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI148277071, Length=474, Percent_Identity=27.2151898734177, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI291045268, Length=470, Percent_Identity=27.2340425531915, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI226437568, Length=141, Percent_Identity=31.2056737588652, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI21389617, Length=141, Percent_Identity=31.2056737588652, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI65787454, Length=141, Percent_Identity=31.2056737588652, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786307, Length=446, Percent_Identity=37.8923766816144, Blast_Score=284, Evalue=7e-78,
Organism=Escherichia coli, GI87082354, Length=464, Percent_Identity=31.0344827586207, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87081717, Length=467, Percent_Identity=27.6231263383298, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=26.8181818181818, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=465, Percent_Identity=52.0430107526882, Blast_Score=466, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI71983429, Length=431, Percent_Identity=27.6102088167053, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=431, Percent_Identity=27.6102088167053, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17557007, Length=480, Percent_Identity=26.25, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71982272, Length=485, Percent_Identity=26.5979381443299, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=226, Percent_Identity=24.7787610619469, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=469, Percent_Identity=48.6140724946695, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6325240, Length=465, Percent_Identity=34.4086021505376, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6325166, Length=459, Percent_Identity=27.8867102396514, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI21358499, Length=466, Percent_Identity=51.2875536480687, Blast_Score=443, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24640553, Length=488, Percent_Identity=27.8688524590164, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24640551, Length=488, Percent_Identity=27.8688524590164, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24640549, Length=488, Percent_Identity=27.8688524590164, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=27.0833333333333, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48885; Mature: 48754

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYDLIVIGGGPGGYKCAIAAAKLGLKVACIDKNSIFGGTCLRVGCIPSKALLHSSYQY
CCCCEEEEEECCCCCCCCEEEEHHHCEEEEEEECCCCCCCCEEEEECCCCHHHHHCCHHH
AHTKNDLSKLGIKIKDASFDLKEMLGYKDARVQELGKGIEYLFNLHKITKINGLASFDQG
HHHHHHHHHCCEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
NLEVSVEGKVLKTKNIVIATGSDVISLPGINIDEKNIISSTGALSLTEVPKKLVVIGAGA
CEEEEECCEEEEECCEEEECCCCEEECCCCCCCCCHHHCCCCCEEHHHCCCEEEEEECCH
IGLEMSSVWSRLGSEVTVVEFFDRIAAAMDGELSKSLLSSLQKQGIKFLLSTKVEEIKQS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SNSLSVKVCSVKDNQTNTIEADKVLVAVGRKPCTESLEKIEKDSRGFVQVNNRYETNVKG
CCCEEEEEEEECCCCCCCEECCEEEEEECCCCHHHHHHHHHCCCCCEEEECCEEECCCCE
IFAIGDVIGGAMLAHKAEEEGVAVAEIIAGQVPHVDYEIIPSVIYTHPAVSSIGKTEEEL
EEEHHHHHHHHHHHHCCHHCCCHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHCCCCHHHH
KSVGRKYKVGKCQFAANGRAKITDDAEGFVKVLTCSRADTILGVHIIGAYADTLINEAAV
HHHHHHCCCCEEEEECCCCCEECCCHHHHEEEEEECCCCCEEEHHHHHHHHHHHHHHHHH
AMAYGAAAEDIYRICHSHPDINEAFRDACIDAFFKK
HHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDYDLIVIGGGPGGYKCAIAAAKLGLKVACIDKNSIFGGTCLRVGCIPSKALLHSSYQY
CCCEEEEEECCCCCCCCEEEEHHHCEEEEEEECCCCCCCCEEEEECCCCHHHHHCCHHH
AHTKNDLSKLGIKIKDASFDLKEMLGYKDARVQELGKGIEYLFNLHKITKINGLASFDQG
HHHHHHHHHCCEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
NLEVSVEGKVLKTKNIVIATGSDVISLPGINIDEKNIISSTGALSLTEVPKKLVVIGAGA
CEEEEECCEEEEECCEEEECCCCEEECCCCCCCCCHHHCCCCCEEHHHCCCEEEEEECCH
IGLEMSSVWSRLGSEVTVVEFFDRIAAAMDGELSKSLLSSLQKQGIKFLLSTKVEEIKQS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SNSLSVKVCSVKDNQTNTIEADKVLVAVGRKPCTESLEKIEKDSRGFVQVNNRYETNVKG
CCCEEEEEEEECCCCCCCEECCEEEEEECCCCHHHHHHHHHCCCCCEEEECCEEECCCCE
IFAIGDVIGGAMLAHKAEEEGVAVAEIIAGQVPHVDYEIIPSVIYTHPAVSSIGKTEEEL
EEEHHHHHHHHHHHHCCHHCCCHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHCCCCHHHH
KSVGRKYKVGKCQFAANGRAKITDDAEGFVKVLTCSRADTILGVHIIGAYADTLINEAAV
HHHHHHCCCCEEEEECCCCCEECCCHHHHEEEEEECCCCCEEEHHHHHHHHHHHHHHHHH
AMAYGAAAEDIYRICHSHPDINEAFRDACIDAFFKK
HHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]