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The map label for this gene is smpB

Identifier: 225630384

GI number: 225630384

Start: 656006

End: 656455

Strand: Direct

Name: smpB

Synonym: WRi_006100

Alternate gene names: 225630384

Gene position: 656006-656455 (Clockwise)

Preceding gene: 225630383

Following gene: 225630385

Centisome position: 45.37

GC content: 33.33

Gene sequence:

>450_bases
ATGGAAGTTATTGCAGAAAATAGGAAAGCAAGGTTTGAATATTTCATCTTAGAAGAATTTGAAGCGGGTATGGTCTTATT
AAGCAGTGAAGTTAAATCGCTAAGAGAAAGAAAAGTAAATATTTCCGACGCCTACGTTGTTGAAAAGAATAGCGAAGTAT
GGCTGCACAATATGCATATTGCAGAATATAAAGCTGCGAACCGAAAAAATCATAAACCAAAAAGGGAACGTAAATTACTC
TTGCATAAAAAGGAGATAAATAAACTAATTGGTCAAATCAAGACTGCTGGGATAACTGTTGTGCCGCTTTCGGTCTATTT
TAACGATAAAGGATTTGCAAAAACTAAAATTGCCATTGTAAAAGGAAAAAAACTCTACGATAAGAGAGCAACCATAAAAC
AGAGAGAATGGGATCGTGAAAAAAGTAGATTGTCTAAAAATAATTTATAG

Upstream 100 bases:

>100_bases
TCAGACTAGCCTGGCACCTTATATTTATTGGGATAACTGTTTTATTATGAATTAAGTCAAAAACTCTGTAATATATTGAA
GCTTAAAGATATCTTTAGAT

Downstream 100 bases:

>100_bases
CAAAATATGTCTTTAAATCCAGTAATTTTCAGCATCGGTCCTGTTTCTATATATTGGTATTCCTTAGCCTACGTTTTGGG
TATAGTGTTTGCATATTGGT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MEVIAENRKARFEYFILEEFEAGMVLLSSEVKSLRERKVNISDAYVVEKNSEVWLHNMHIAEYKAANRKNHKPKRERKLL
LHKKEINKLIGQIKTAGITVVPLSVYFNDKGFAKTKIAIVKGKKLYDKRATIKQREWDREKSRLSKNNL

Sequences:

>Translated_149_residues
MEVIAENRKARFEYFILEEFEAGMVLLSSEVKSLRERKVNISDAYVVEKNSEVWLHNMHIAEYKAANRKNHKPKRERKLL
LHKKEINKLIGQIKTAGITVVPLSVYFNDKGFAKTKIAIVKGKKLYDKRATIKQREWDREKSRLSKNNL
>Mature_149_residues
MEVIAENRKARFEYFILEEFEAGMVLLSSEVKSLRERKVNISDAYVVEKNSEVWLHNMHIAEYKAANRKNHKPKRERKLL
LHKKEINKLIGQIKTAGITVVPLSVYFNDKGFAKTKIAIVKGKKLYDKRATIKQREWDREKSRLSKNNL

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=148, Percent_Identity=47.2972972972973, Blast_Score=127, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_WOLPM (Q73H09)

Other databases:

- EMBL:   AE017196
- RefSeq:   NP_966523.1
- ProteinModelPortal:   Q73H09
- SMR:   Q73H09
- EnsemblBacteria:   EBWOLT00000004191
- GeneID:   2738044
- GenomeReviews:   AE017196_GR
- KEGG:   wol:WD0767
- TIGR:   WD_0767
- GeneTree:   EBGT00050000031219
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   Q73H09
- ProtClustDB:   PRK05422
- BioCyc:   WPIP955:WD_0767-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17514; Mature: 17514

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVIAENRKARFEYFILEEFEAGMVLLSSEVKSLRERKVNISDAYVVEKNSEVWLHNMHI
CCCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCH
AEYKAANRKNHKPKRERKLLLHKKEINKLIGQIKTAGITVVPLSVYFNDKGFAKTKIAIV
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCEEEEEEE
KGKKLYDKRATIKQREWDREKSRLSKNNL
ECCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEVIAENRKARFEYFILEEFEAGMVLLSSEVKSLRERKVNISDAYVVEKNSEVWLHNMHI
CCCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCH
AEYKAANRKNHKPKRERKLLLHKKEINKLIGQIKTAGITVVPLSVYFNDKGFAKTKIAIV
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCEEEEEEE
KGKKLYDKRATIKQREWDREKSRLSKNNL
ECCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA