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The map label for this gene is purN [H]

Identifier: 225630380

GI number: 225630380

Start: 652159

End: 652725

Strand: Reverse

Name: purN [H]

Synonym: WRi_006060

Alternate gene names: 225630380

Gene position: 652725-652159 (Counterclockwise)

Preceding gene: 225630382

Following gene: 225630375

Centisome position: 45.14

GC content: 37.74

Gene sequence:

>567_bases
ATGAAAAAGATAAAACTCGGAATACTAATTTCAGGTAGGGGGTCGAATATGCAGGCCTTAATAGAAGCATGTCAAGATCA
GAATTTTTCCGCAGAAGTTGCATGTGTTATCACAAATAATAGCGAAGCTGCTGGTCTGAAAATAGCAGAGCAAGCAGGAA
TCTCAGCGTTTATTGTTAAAGATAAACCGCTTGATGCTGGTAAAATTCACGAAATACTTGTTCAACATAAGGTTGATTTA
ATTTGCCTTGCAGGGTTTATGAGAATTCTCAAAGCTGATTTTCTGAGTAAATGGCATAACAAGGTGATAAATATTCATCC
CTCTTTACTTCCGTCTTTTAAAGGCCTAAACGCTCAAGAGCAAGCTCTAAAAGCAGGTGTAAAAATTACAGGTTGTACTG
TACATTATGTCACTCCTGAAGTTGATGCTGGAGCCATAATTGCTCAAGTTGTTGTACCAGTGTTACCAGCTGATGATATT
CAAAGCCTCTCAGAACGCATTCTAGCTGAGGAGCATAAGTGCTATGTAGAAGCAGTAAGATCAATAGCAGAAAGTATTAA
TTATTGA

Upstream 100 bases:

>100_bases
CTTATATAAAGAGTTTAGTCTGCCTTCTTTGCTATAAAATAAGTTTTCCTTTTGAGCTGTTTTGTTAGTATTAACTAAAT
TCTAAACTAACTGTGCTATA

Downstream 100 bases:

>100_bases
CCCTGAGCGTTTTTTCCAACTTCAACGAAAAATAAATTTTCGGGTTCCAGTAAAGAACTTGCCAGCTCGTTTACTTTTTC
TAGCTTTACGTCATTAATGA

Product: phosphoribosylglycinamide formyltransferase, putative

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MKKIKLGILISGRGSNMQALIEACQDQNFSAEVACVITNNSEAAGLKIAEQAGISAFIVKDKPLDAGKIHEILVQHKVDL
ICLAGFMRILKADFLSKWHNKVINIHPSLLPSFKGLNAQEQALKAGVKITGCTVHYVTPEVDAGAIIAQVVVPVLPADDI
QSLSERILAEEHKCYVEAVRSIAESINY

Sequences:

>Translated_188_residues
MKKIKLGILISGRGSNMQALIEACQDQNFSAEVACVITNNSEAAGLKIAEQAGISAFIVKDKPLDAGKIHEILVQHKVDL
ICLAGFMRILKADFLSKWHNKVINIHPSLLPSFKGLNAQEQALKAGVKITGCTVHYVTPEVDAGAIIAQVVVPVLPADDI
QSLSERILAEEHKCYVEAVRSIAESINY
>Mature_188_residues
MKKIKLGILISGRGSNMQALIEACQDQNFSAEVACVITNNSEAAGLKIAEQAGISAFIVKDKPLDAGKIHEILVQHKVDL
ICLAGFMRILKADFLSKWHNKVINIHPSLLPSFKGLNAQEQALKAGVKITGCTVHYVTPEVDAGAIIAQVVVPVLPADDI
QSLSERILAEEHKCYVEAVRSIAESINY

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=188, Percent_Identity=48.936170212766, Blast_Score=181, Evalue=3e-46,
Organism=Homo sapiens, GI209869995, Length=188, Percent_Identity=48.936170212766, Blast_Score=181, Evalue=3e-46,
Organism=Homo sapiens, GI209869993, Length=188, Percent_Identity=48.936170212766, Blast_Score=181, Evalue=3e-46,
Organism=Escherichia coli, GI1788846, Length=173, Percent_Identity=39.3063583815029, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1787483, Length=156, Percent_Identity=32.0512820512821, Blast_Score=81, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=188, Percent_Identity=40.4255319148936, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320616, Length=211, Percent_Identity=27.4881516587678, Blast_Score=73, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582400, Length=185, Percent_Identity=46.4864864864865, Blast_Score=172, Evalue=9e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20355; Mature: 20355

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIKLGILISGRGSNMQALIEACQDQNFSAEVACVITNNSEAAGLKIAEQAGISAFIVK
CCEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEHHHCCCEEEEEE
DKPLDAGKIHEILVQHKVDLICLAGFMRILKADFLSKWHNKVINIHPSLLPSFKGLNAQE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHCCCCCCCCHHH
QALKAGVKITGCTVHYVTPEVDAGAIIAQVVVPVLPADDIQSLSERILAEEHKCYVEAVR
HHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
SIAESINY
HHHHHCCC
>Mature Secondary Structure
MKKIKLGILISGRGSNMQALIEACQDQNFSAEVACVITNNSEAAGLKIAEQAGISAFIVK
CCEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEHHHCCCEEEEEE
DKPLDAGKIHEILVQHKVDLICLAGFMRILKADFLSKWHNKVINIHPSLLPSFKGLNAQE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHCCCCCCCCHHH
QALKAGVKITGCTVHYVTPEVDAGAIIAQVVVPVLPADDIQSLSERILAEEHKCYVEAVR
HHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
SIAESINY
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]