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The map label for this gene is ltaA [H]

Identifier: 225630357

GI number: 225630357

Start: 623406

End: 624458

Strand: Direct

Name: ltaA [H]

Synonym: WRi_005800

Alternate gene names: 225630357

Gene position: 623406-624458 (Clockwise)

Preceding gene: 225630356

Following gene: 225630358

Centisome position: 43.12

GC content: 35.52

Gene sequence:

>1053_bases
ATGAGAGTAAAAATAGATTTCTTTAGCGATTCAAACACTGACCCTTCAGCTAAAATGAGGGAAGCGATGGCTAAAGCAAA
AGTTGGAAACGAAGCTGCATGTGAAGATCCAACTGTGAACGAATTAGTGGCAACCGCATGTAAAATATTAGGTAAACCTG
CAGGAATCTTTTTGATTTCAGGAACGATGTCCAATGTAATTGCTTATAAAGTACACATTCAAAGACCTGGTGATTACTTG
CTGCTTGATGAAACTTCACATCCACTCATAGTACAATCTGGCTTGATCGCAGCCCAGGCACATGCTACTCCTTTGCCCAT
CAAAGGTACAAGAGGAATATTTACTGGAGAACAAATTGTTGAGTTTATTACTCGTCCTAGTTTAAGAAATATTCCGAGAG
TTGGGCTTGTTTCTATCGAACAAACGACAAATTTTGGTGGTGGAGCTGTGTGGCCTTTAGAATATATTCAAGAGATCTCA
AGTCTCTGCAAAGAGAATGACGTATTCACTCATTTAGATGGAGCAAGACTGTTCAACGCTTGTGCTCATACTAAAATATC
TCCAAAGGAGTATGCAAGTTATTTTGATTCGGTATTCATTGATTTTAGTAAAGGATTAGGTGCTCCAATGGGTGCAATAT
TGCTTGGAAGTGAAGATTTTATTGAAAAAGCTTGGTACTATAAGTTTCAAATTGGCGGAGGTATGCACCAAGCTGGCATT
ATTTCAGCTGCATGTTTATATGCTCTACGTAATAATGTAAATTCTTTAGAAGAAGATCATAAAAAAATGCAGCACCTTAT
AGAAGGCCTAGACTCCATAGATCAAGTTATAATTGATATCGACCTGTATAAAACTAATATAGCGTATTTTTCATTACGTA
CTAATTGTATATCTACTCAAGAATTAATAAACGTACTAATGACAAATTATGGAATTAGAATGGCTAACATCAAAGGTAAA
ATCAGAGCTATAACACATAGAGATATAAGCTATGAAGATATTAATACTACTGTATCTGCTATAAGAAAGATACTAAGTAA
TTTTCTGCACTAA

Upstream 100 bases:

>100_bases
GGCTCGAGTCTCATATAAGAGCAGTTACCCATCTTGGCACAAAGGAAACAGACATCATGAAAGCTGCTGAAATTATAAAG
GAGGTTGTGTTAAATTTAAT

Downstream 100 bases:

>100_bases
TTGTTTGCAAACTTCTTGTCAAACAATATTATACCAATATAGTATTATGCTAGCATCTGCATAGAGCTATTTGGGAATAG
GTGTGAACTTTGTCTTCTTT

Product: L-allo-threonine aldolase, putative

Products: Acetaldehyde; Glycine [C]

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRVKIDFFSDSNTDPSAKMREAMAKAKVGNEAACEDPTVNELVATACKILGKPAGIFLISGTMSNVIAYKVHIQRPGDYL
LLDETSHPLIVQSGLIAAQAHATPLPIKGTRGIFTGEQIVEFITRPSLRNIPRVGLVSIEQTTNFGGGAVWPLEYIQEIS
SLCKENDVFTHLDGARLFNACAHTKISPKEYASYFDSVFIDFSKGLGAPMGAILLGSEDFIEKAWYYKFQIGGGMHQAGI
ISAACLYALRNNVNSLEEDHKKMQHLIEGLDSIDQVIIDIDLYKTNIAYFSLRTNCISTQELINVLMTNYGIRMANIKGK
IRAITHRDISYEDINTTVSAIRKILSNFLH

Sequences:

>Translated_350_residues
MRVKIDFFSDSNTDPSAKMREAMAKAKVGNEAACEDPTVNELVATACKILGKPAGIFLISGTMSNVIAYKVHIQRPGDYL
LLDETSHPLIVQSGLIAAQAHATPLPIKGTRGIFTGEQIVEFITRPSLRNIPRVGLVSIEQTTNFGGGAVWPLEYIQEIS
SLCKENDVFTHLDGARLFNACAHTKISPKEYASYFDSVFIDFSKGLGAPMGAILLGSEDFIEKAWYYKFQIGGGMHQAGI
ISAACLYALRNNVNSLEEDHKKMQHLIEGLDSIDQVIIDIDLYKTNIAYFSLRTNCISTQELINVLMTNYGIRMANIKGK
IRAITHRDISYEDINTTVSAIRKILSNFLH
>Mature_350_residues
MRVKIDFFSDSNTDPSAKMREAMAKAKVGNEAACEDPTVNELVATACKILGKPAGIFLISGTMSNVIAYKVHIQRPGDYL
LLDETSHPLIVQSGLIAAQAHATPLPIKGTRGIFTGEQIVEFITRPSLRNIPRVGLVSIEQTTNFGGGAVWPLEYIQEIS
SLCKENDVFTHLDGARLFNACAHTKISPKEYASYFDSVFIDFSKGLGAPMGAILLGSEDFIEKAWYYKFQIGGGMHQAGI
ISAACLYALRNNVNSLEEDHKKMQHLIEGLDSIDQVIIDIDLYKTNIAYFSLRTNCISTQELINVLMTNYGIRMANIKGK
IRAITHRDISYEDINTTVSAIRKILSNFLH

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=346, Percent_Identity=34.393063583815, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI193206520, Length=353, Percent_Identity=32.2946175637394, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI193206522, Length=353, Percent_Identity=32.2946175637394, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI193206524, Length=353, Percent_Identity=32.2946175637394, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320789, Length=252, Percent_Identity=33.7301587301587, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21355439, Length=349, Percent_Identity=32.6647564469914, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5 [C]

Molecular weight: Translated: 38682; Mature: 38682

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVKIDFFSDSNTDPSAKMREAMAKAKVGNEAACEDPTVNELVATACKILGKPAGIFLIS
CEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
GTMSNVIAYKVHIQRPGDYLLLDETSHPLIVQSGLIAAQAHATPLPIKGTRGIFTGEQIV
CCCCCEEEEEEEEECCCCEEEEECCCCCEEEECCCEEEECCCCCCCCCCCCCCCCHHHHH
EFITRPSLRNIPRVGLVSIEQTTNFGGGAVWPLEYIQEISSLCKENDVFTHLDGARLFNA
HHHCCCCHHCCCCCCEEEEECCCCCCCCEECHHHHHHHHHHHHHCCCCEEECCHHHHHHH
CAHTKISPKEYASYFDSVFIDFSKGLGAPMGAILLGSEDFIEKAWYYKFQIGGGMHQAGI
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHEEEEEEECCCCCHHHH
ISAACLYALRNNVNSLEEDHKKMQHLIEGLDSIDQVIIDIDLYKTNIAYFSLRTNCISTQ
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEEEEEECCCCCHH
ELINVLMTNYGIRMANIKGKIRAITHRDISYEDINTTVSAIRKILSNFLH
HHHHHHHHHCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVKIDFFSDSNTDPSAKMREAMAKAKVGNEAACEDPTVNELVATACKILGKPAGIFLIS
CEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
GTMSNVIAYKVHIQRPGDYLLLDETSHPLIVQSGLIAAQAHATPLPIKGTRGIFTGEQIV
CCCCCEEEEEEEEECCCCEEEEECCCCCEEEECCCEEEECCCCCCCCCCCCCCCCHHHHH
EFITRPSLRNIPRVGLVSIEQTTNFGGGAVWPLEYIQEISSLCKENDVFTHLDGARLFNA
HHHCCCCHHCCCCCCEEEEECCCCCCCCEECHHHHHHHHHHHHHCCCCEEECCHHHHHHH
CAHTKISPKEYASYFDSVFIDFSKGLGAPMGAILLGSEDFIEKAWYYKFQIGGGMHQAGI
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHEEEEEEECCCCCHHHH
ISAACLYALRNNVNSLEEDHKKMQHLIEGLDSIDQVIIDIDLYKTNIAYFSLRTNCISTQ
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEEEEEECCCCCHH
ELINVLMTNYGIRMANIKGKIRAITHRDISYEDINTTVSAIRKILSNFLH
HHHHHHHHHCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-Threonine [C]

Specific reaction: L-Threonine <==> Acetaldehyde + Glycine [C]

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]