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The map label for this gene is ltaA [H]

Identifier: 225630356

GI number: 225630356

Start: 622360

End: 623409

Strand: Direct

Name: ltaA [H]

Synonym: WRi_005790

Alternate gene names: 225630356

Gene position: 622360-623409 (Clockwise)

Preceding gene: 225630355

Following gene: 225630357

Centisome position: 43.04

GC content: 33.52

Gene sequence:

>1050_bases
ATGGAAAAAATTATTGATCTGAGAAGTGACACTACAACTCTGCAGAGCTCCAACGTTATACATGCAATAAGTAATGCAAC
TGTTGGAGATTTTGCTTATGGTGAAGACAAAAGCTGTAATGACTTATCAGAATATTGCAAACAATTATTCAAAGTAGAAG
AAGCATTATTTGCAACCAGCGGTATGCTTGCAAATAGATTAGCCATTGCCAGTCAAACAAGTCCTGGTGATGAACTAATA
ACTCATTATAATTATCACGTTAATTTTTTCGATAGCGCAGGCAATGCAAAAGTAAATAATATCGTATTTAATTGTATTAG
AAATAATAGCGGAATCTTAGATGTTGCTGAAGTAGAATATGCTATTAATTCTAAGCCAAGGTACAAAATTTTTGCTCAGG
TGAGTCTTGTTTCTATAGAAAATAGTATAAACGGGTTTAATGGTAAAATTTATCCTTTTGAAAAACAAGTCGAGTTATAT
CACTTTTTAAAAGCTCACAATGTAAATCTTCATTTAGACGGTGCAAGAATATTTAATGCTCATGTCGAAACAAATATTGC
TTTAGCAGATTATGCTAAATATGTGGATACAATGAGCTTTTCTTTTACCAAAGGACTTGGAGCTCCTTTTGGTTCAATGC
TTATGGGTAAAAGAGAGATAATTGAAAGGGCTAAAAAGCTACAGGTTTGGCTAGGTAGTGGTTATCATCAGATAGGGTAT
TGTGCTAATGCAGCAAAGTATGTACTACAGAACAATATTTCTAGGCTTAAAGAAGACAATAAACTCGCCAAACTGTTTGC
AGACAAGATTAAGGAAATTCCACACATAAAACTGGTACTACCTTATCCAGAAACTAATATAGTAAGCTTTAGTATAAAAG
CACTAAATGTAACTAATGAGGTTTTCTTGAGTAAGTGCCAAGCTTATGGTTTATTGCTTTTTCCTTGGCTCGAGTCTCAT
ATAAGAGCAGTTACCCATCTTGGCACAAAGGAAACAGACATCATGAAAGCTGCTGAAATTATAAAGGAGGTTGTGTTAAA
TTTAATATGA

Upstream 100 bases:

>100_bases
ATTGTCCAAGATGGTAAACACGCTGAATTAGTTTCTAAGCAAGGTCTTTACAAGGAACTTTGGAATGCTCAAATTGGTTG
TTTGAATGGCAAAAAATAAG

Downstream 100 bases:

>100_bases
GAGTAAAAATAGATTTCTTTAGCGATTCAAACACTGACCCTTCAGCTAAAATGAGGGAAGCGATGGCTAAAGCAAAAGTT
GGAAACGAAGCTGCATGTGA

Product: L-allo-threonine aldolase, putative

Products: Acetaldehyde; Glycine [C]

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MEKIIDLRSDTTTLQSSNVIHAISNATVGDFAYGEDKSCNDLSEYCKQLFKVEEALFATSGMLANRLAIASQTSPGDELI
THYNYHVNFFDSAGNAKVNNIVFNCIRNNSGILDVAEVEYAINSKPRYKIFAQVSLVSIENSINGFNGKIYPFEKQVELY
HFLKAHNVNLHLDGARIFNAHVETNIALADYAKYVDTMSFSFTKGLGAPFGSMLMGKREIIERAKKLQVWLGSGYHQIGY
CANAAKYVLQNNISRLKEDNKLAKLFADKIKEIPHIKLVLPYPETNIVSFSIKALNVTNEVFLSKCQAYGLLLFPWLESH
IRAVTHLGTKETDIMKAAEIIKEVVLNLI

Sequences:

>Translated_349_residues
MEKIIDLRSDTTTLQSSNVIHAISNATVGDFAYGEDKSCNDLSEYCKQLFKVEEALFATSGMLANRLAIASQTSPGDELI
THYNYHVNFFDSAGNAKVNNIVFNCIRNNSGILDVAEVEYAINSKPRYKIFAQVSLVSIENSINGFNGKIYPFEKQVELY
HFLKAHNVNLHLDGARIFNAHVETNIALADYAKYVDTMSFSFTKGLGAPFGSMLMGKREIIERAKKLQVWLGSGYHQIGY
CANAAKYVLQNNISRLKEDNKLAKLFADKIKEIPHIKLVLPYPETNIVSFSIKALNVTNEVFLSKCQAYGLLLFPWLESH
IRAVTHLGTKETDIMKAAEIIKEVVLNLI
>Mature_349_residues
MEKIIDLRSDTTTLQSSNVIHAISNATVGDFAYGEDKSCNDLSEYCKQLFKVEEALFATSGMLANRLAIASQTSPGDELI
THYNYHVNFFDSAGNAKVNNIVFNCIRNNSGILDVAEVEYAINSKPRYKIFAQVSLVSIENSINGFNGKIYPFEKQVELY
HFLKAHNVNLHLDGARIFNAHVETNIALADYAKYVDTMSFSFTKGLGAPFGSMLMGKREIIERAKKLQVWLGSGYHQIGY
CANAAKYVLQNNISRLKEDNKLAKLFADKIKEIPHIKLVLPYPETNIVSFSIKALNVTNEVFLSKCQAYGLLLFPWLESH
IRAVTHLGTKETDIMKAAEIIKEVVLNLI

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=270, Percent_Identity=37.7777777777778, Blast_Score=175, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI193206524, Length=269, Percent_Identity=34.9442379182156, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI193206522, Length=269, Percent_Identity=34.9442379182156, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI193206520, Length=269, Percent_Identity=34.9442379182156, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6320789, Length=312, Percent_Identity=29.4871794871795, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI21355439, Length=313, Percent_Identity=27.7955271565495, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5 [C]

Molecular weight: Translated: 39101; Mature: 39101

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIIDLRSDTTTLQSSNVIHAISNATVGDFAYGEDKSCNDLSEYCKQLFKVEEALFATS
CCCCEECCCCCCEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GMLANRLAIASQTSPGDELITHYNYHVNFFDSAGNAKVNNIVFNCIRNNSGILDVAEVEY
HHHHHHHEEECCCCCCHHHEEEEEEEEEEEECCCCCCHHHEEEEEEECCCCEEEHHHHHH
AINSKPRYKIFAQVSLVSIENSINGFNGKIYPFEKQVELYHFLKAHNVNLHLDGARIFNA
HHCCCCCEEEEEEEEEEEECCCCCCCCCEEECHHHHHHHHHHHHHCCCEEEECCCEEEEE
HVETNIALADYAKYVDTMSFSFTKGLGAPFGSMLMGKREIIERAKKLQVWLGSGYHQIGY
EHHCCCHHHHHHHHHHHHCHHHHCCCCCCHHHHHHCHHHHHHHHHHEEHEECCCCHHHHH
CANAAKYVLQNNISRLKEDNKLAKLFADKIKEIPHIKLVLPYPETNIVSFSIKALNVTNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEECCHHH
VFLSKCQAYGLLLFPWLESHIRAVTHLGTKETDIMKAAEIIKEVVLNLI
HHHHHHHHCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKIIDLRSDTTTLQSSNVIHAISNATVGDFAYGEDKSCNDLSEYCKQLFKVEEALFATS
CCCCEECCCCCCEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GMLANRLAIASQTSPGDELITHYNYHVNFFDSAGNAKVNNIVFNCIRNNSGILDVAEVEY
HHHHHHHEEECCCCCCHHHEEEEEEEEEEEECCCCCCHHHEEEEEEECCCCEEEHHHHHH
AINSKPRYKIFAQVSLVSIENSINGFNGKIYPFEKQVELYHFLKAHNVNLHLDGARIFNA
HHCCCCCEEEEEEEEEEEECCCCCCCCCEEECHHHHHHHHHHHHHCCCEEEECCCEEEEE
HVETNIALADYAKYVDTMSFSFTKGLGAPFGSMLMGKREIIERAKKLQVWLGSGYHQIGY
EHHCCCHHHHHHHHHHHHCHHHHCCCCCCHHHHHHCHHHHHHHHHHEEHEECCCCHHHHH
CANAAKYVLQNNISRLKEDNKLAKLFADKIKEIPHIKLVLPYPETNIVSFSIKALNVTNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEECCHHH
VFLSKCQAYGLLLFPWLESHIRAVTHLGTKETDIMKAAEIIKEVVLNLI
HHHHHHHHCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-Threonine [C]

Specific reaction: L-Threonine <==> Acetaldehyde + Glycine [C]

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]