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The map label for this gene is galE [H]

Identifier: 225630351

GI number: 225630351

Start: 614682

End: 615641

Strand: Direct

Name: galE [H]

Synonym: WRi_005740

Alternate gene names: 225630351

Gene position: 614682-615641 (Clockwise)

Preceding gene: 225630350

Following gene: 225630352

Centisome position: 42.51

GC content: 33.23

Gene sequence:

>960_bases
ATGGGAATATTAATAACAGGTGCTGCAGGTTTGATAGGATCGGCTTTAGTAGAAAAGTTAGAAAAGCAGGGTTATGAAGT
AATAAGCTGCGATATTAGGTTTCGCGACAACCCACTCAGTTTTTTTTCAGAAGATATAATGCCGCTACTCGCTAAGTGTA
CGGGGGTTATTCATCTGGCTGCAATTTCTAGAGTTATACATGGTGAGCTTTATCCTGAATTGTGCCAAAAAGTTAATGTC
GATGGTACAATCCAATTCTTAGAATTCTGCAAGTCGCTTCCGAATAAACCATGGTTTATATATGCAAGCAGTAGAGAAGT
ATATGGAGAACAAAAGGAACTACCAGTTGCAGAGTCTGCTAGTATCAACCCAATAAATAATTACGCCAAAGGTAAAGCAT
TTATTGAAGAACAAGTAATAAATTTAAAAGGTTCTAACTTTAATGTAGCAATATTACGCTTCTCAAATGTATATGGTGGT
TTACTAGATCATAGTAGTAGAGTAATTCCTGCACTCTGTATCAATGCATTGAAAGGCGATCCAATTAGGATAGAAGGTAA
AGAATGTGTTTTTGATTTTACCTATTTGGATGATGTTATAGAGGGTATATACTTAACTGTTAAATATTTACAAAACGAGA
AATCTTCTCTTCCTGCTATTCATCTTACTACTAATAGCCCATGTACTTTAGAAAACTTAGCAAAAACAATATTAAAAGTC
ACGGAAAGTGATTCTAGAATTGATTTTTATCCTCCAAGAAATTTTGACGTAACTAAGTTCCATGGTGATTTTACTAGAGC
TAAAGAGCTACTTGGTTGGTCTCCAAAGCATTCATTAAAAGTAGGATTAGGTAAATTTATAAAAAGTCTACAAAACAATA
CACAAGAATATCCTAACAATATAGATATGGTAATATATGAAAATATTAAAAGTTATTCATGGTTACCCTCCTTATTATAG

Upstream 100 bases:

>100_bases
AAGTTGACATTTGTTTTATACTAGTTTTATAATGCTTTTTAAGTCTTACTTGTAAGATGATAGGTGTGAAAGATTTTCCT
GGTTGTAGTGAGAGGGACAC

Downstream 100 bases:

>100_bases
CGCTGGTTCGGAGGTTTACAGCCAAACTCTTGCCCATGAACTAGCCAATAATAATGAGGTACAAATATTTACTAGGCATG
AAAACAGCTTCTTACCTGAT

Product: NAD-dependent epimerase/dehydratase family protein

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MGILITGAAGLIGSALVEKLEKQGYEVISCDIRFRDNPLSFFSEDIMPLLAKCTGVIHLAAISRVIHGELYPELCQKVNV
DGTIQFLEFCKSLPNKPWFIYASSREVYGEQKELPVAESASINPINNYAKGKAFIEEQVINLKGSNFNVAILRFSNVYGG
LLDHSSRVIPALCINALKGDPIRIEGKECVFDFTYLDDVIEGIYLTVKYLQNEKSSLPAIHLTTNSPCTLENLAKTILKV
TESDSRIDFYPPRNFDVTKFHGDFTRAKELLGWSPKHSLKVGLGKFIKSLQNNTQEYPNNIDMVIYENIKSYSWLPSLL

Sequences:

>Translated_319_residues
MGILITGAAGLIGSALVEKLEKQGYEVISCDIRFRDNPLSFFSEDIMPLLAKCTGVIHLAAISRVIHGELYPELCQKVNV
DGTIQFLEFCKSLPNKPWFIYASSREVYGEQKELPVAESASINPINNYAKGKAFIEEQVINLKGSNFNVAILRFSNVYGG
LLDHSSRVIPALCINALKGDPIRIEGKECVFDFTYLDDVIEGIYLTVKYLQNEKSSLPAIHLTTNSPCTLENLAKTILKV
TESDSRIDFYPPRNFDVTKFHGDFTRAKELLGWSPKHSLKVGLGKFIKSLQNNTQEYPNNIDMVIYENIKSYSWLPSLL
>Mature_318_residues
GILITGAAGLIGSALVEKLEKQGYEVISCDIRFRDNPLSFFSEDIMPLLAKCTGVIHLAAISRVIHGELYPELCQKVNVD
GTIQFLEFCKSLPNKPWFIYASSREVYGEQKELPVAESASINPINNYAKGKAFIEEQVINLKGSNFNVAILRFSNVYGGL
LDHSSRVIPALCINALKGDPIRIEGKECVFDFTYLDDVIEGIYLTVKYLQNEKSSLPAIHLTTNSPCTLENLAKTILKVT
ESDSRIDFYPPRNFDVTKFHGDFTRAKELLGWSPKHSLKVGLGKFIKSLQNNTQEYPNNIDMVIYENIKSYSWLPSLL

Specific function: Can have functions other than metabolism of galactose. It also could be involved in preparation of carbohydrate residues for incorporation into complex polymers, such as exopolysaccharides [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=318, Percent_Identity=27.9874213836478, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1786974, Length=344, Percent_Identity=23.546511627907, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788353, Length=367, Percent_Identity=25.3405994550409, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539532, Length=307, Percent_Identity=29.6416938110749, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71982035, Length=331, Percent_Identity=24.773413897281, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=333, Percent_Identity=24.6246246246246, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21356223, Length=312, Percent_Identity=29.1666666666667, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR005886
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 35781; Mature: 35649

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGILITGAAGLIGSALVEKLEKQGYEVISCDIRFRDNPLSFFSEDIMPLLAKCTGVIHLA
CCEEEECHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
AISRVIHGELYPELCQKVNVDGTIQFLEFCKSLPNKPWFIYASSREVYGEQKELPVAESA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCCCCCCCC
SINPINNYAKGKAFIEEQVINLKGSNFNVAILRFSNVYGGLLDHSSRVIPALCINALKGD
CCCCCHHHHHHHHHHHHHHHEECCCCEEEEEEEECCHHHHHHCCCCCHHHHHHHHHHCCC
PIRIEGKECVFDFTYLDDVIEGIYLTVKYLQNEKSSLPAIHLTTNSPCTLENLAKTILKV
CEEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHH
TESDSRIDFYPPRNFDVTKFHGDFTRAKELLGWSPKHSLKVGLGKFIKSLQNNTQEYPNN
CCCCCEEEECCCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCCC
IDMVIYENIKSYSWLPSLL
CCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
GILITGAAGLIGSALVEKLEKQGYEVISCDIRFRDNPLSFFSEDIMPLLAKCTGVIHLA
CEEEECHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
AISRVIHGELYPELCQKVNVDGTIQFLEFCKSLPNKPWFIYASSREVYGEQKELPVAESA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCCCCCCCC
SINPINNYAKGKAFIEEQVINLKGSNFNVAILRFSNVYGGLLDHSSRVIPALCINALKGD
CCCCCHHHHHHHHHHHHHHHEECCCCEEEEEEEECCHHHHHHCCCCCHHHHHHHHHHCCC
PIRIEGKECVFDFTYLDDVIEGIYLTVKYLQNEKSSLPAIHLTTNSPCTLENLAKTILKV
CEEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHH
TESDSRIDFYPPRNFDVTKFHGDFTRAKELLGWSPKHSLKVGLGKFIKSLQNNTQEYPNN
CCCCCEEEECCCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCCC
IDMVIYENIKSYSWLPSLL
CCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788721 [H]