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The map label for this gene is rpoA [H]

Identifier: 225630278

GI number: 225630278

Start: 540209

End: 541276

Strand: Reverse

Name: rpoA [H]

Synonym: WRi_004960

Alternate gene names: 225630278

Gene position: 541276-540209 (Counterclockwise)

Preceding gene: 225630279

Following gene: 225630277

Centisome position: 37.44

GC content: 35.39

Gene sequence:

>1068_bases
ATGTATTATAATAATGATGTTTCTCTCTGTAGCAATTTAGATAAATTGATTAAACCTAGTTCAGTTAAGGTAATATCAGA
TGATTCAAGTGAAAAAAGTGATATAGTGCTAGAACCGTTGGAAAGTGGTTTTGCTTTAACATTAGGTAATGCATTAAGAC
GTGTAATGTTATCTTCTCTTAAAGGTAGTGCTGTTTATGGAATAAAAATTGAAGGTGTAACTCATGAGTTCACTTCAATT
CAAGGGGTGAGAGAAGACGTAACTGATATAGTATTAAATATGGGTATGTTGAGGTGTAAATTAAATGGTGCTTCTAATAA
ATGCTTAAATTTAAACGCTAAAGGGCCTTGCCAGGTATTAGCTGGGATGATAGAAACTGATGATCAATGCTCTATTGTTA
ATAAAGATTTGTTAATATGTACGCTGGGGCAAAATGTGGAGCTTAACATGACCATATACGTGGCTAGCGGAAAAGGTTAT
CTTCCTGTAACTAAATATAAAGAAAATGAACTTTTGAAGCTTATGAGTGAACAGGATTTAATTGGTTTTATTCCAGTTAA
TGCTTTGTATAGCCCTGTCAACAGGGTTTCGTACAGGGTAGAGAACAGTCGTGTTGGCCAAGTGACTGATAAGGATAAGC
TGATATTGTCAATTGAAACTGATGGTACAATTTCTCCGAGTCAAGCTGTTGACTCTGCTGCAAGAATATTACAGGAGCAA
CTTCAGCCCTTTATTAGTTCTGATGTAAGTTATAAAAAATCGCAGGTTTCTTCGTCTAGTGGTGCTAAAGATTTGGGTTA
TGACCCTGTCTTATTGCGTAAGGTAGATGAAATGGAATTATCTGTCAGGTCTCATAACTGTCTGAAAAACGAAAACATCA
CTTATATAGGTGATCTTGTGCAAAAGACAGAAGGTGAAATGTTAAGAACTGCTAATTTTGGCAGAAAGTCTTTGAATGAG
ATTAAGGCAGTTTTGACTAATTTTGGCTTGTCTTTGGGTATGAATGTGCTAAACTGGCCACCTAAGGATATAGATGAGTT
GGCTAAACAACATACTGATGAAGATTAG

Upstream 100 bases:

>100_bases
GTTGACTGTGACTTCAATTGCAGATAAAACCGCAATTCCTCATAATGGGTGCAGGTTAAGAAAAAAAAGAAGAGTATAGG
ATATTTGAAAAGGTTGTTTT

Downstream 100 bases:

>100_bases
GTGATATATGAAACATGGAATAAAAAAACGTAAACTATCTCGTTGTACTGAACATAGATTATCGACGTTAAAGAATCTAT
CTATCTCATTAATTAACCAT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MYYNNDVSLCSNLDKLIKPSSVKVISDDSSEKSDIVLEPLESGFALTLGNALRRVMLSSLKGSAVYGIKIEGVTHEFTSI
QGVREDVTDIVLNMGMLRCKLNGASNKCLNLNAKGPCQVLAGMIETDDQCSIVNKDLLICTLGQNVELNMTIYVASGKGY
LPVTKYKENELLKLMSEQDLIGFIPVNALYSPVNRVSYRVENSRVGQVTDKDKLILSIETDGTISPSQAVDSAARILQEQ
LQPFISSDVSYKKSQVSSSSGAKDLGYDPVLLRKVDEMELSVRSHNCLKNENITYIGDLVQKTEGEMLRTANFGRKSLNE
IKAVLTNFGLSLGMNVLNWPPKDIDELAKQHTDED

Sequences:

>Translated_355_residues
MYYNNDVSLCSNLDKLIKPSSVKVISDDSSEKSDIVLEPLESGFALTLGNALRRVMLSSLKGSAVYGIKIEGVTHEFTSI
QGVREDVTDIVLNMGMLRCKLNGASNKCLNLNAKGPCQVLAGMIETDDQCSIVNKDLLICTLGQNVELNMTIYVASGKGY
LPVTKYKENELLKLMSEQDLIGFIPVNALYSPVNRVSYRVENSRVGQVTDKDKLILSIETDGTISPSQAVDSAARILQEQ
LQPFISSDVSYKKSQVSSSSGAKDLGYDPVLLRKVDEMELSVRSHNCLKNENITYIGDLVQKTEGEMLRTANFGRKSLNE
IKAVLTNFGLSLGMNVLNWPPKDIDELAKQHTDED
>Mature_355_residues
MYYNNDVSLCSNLDKLIKPSSVKVISDDSSEKSDIVLEPLESGFALTLGNALRRVMLSSLKGSAVYGIKIEGVTHEFTSI
QGVREDVTDIVLNMGMLRCKLNGASNKCLNLNAKGPCQVLAGMIETDDQCSIVNKDLLICTLGQNVELNMTIYVASGKGY
LPVTKYKENELLKLMSEQDLIGFIPVNALYSPVNRVSYRVENSRVGQVTDKDKLILSIETDGTISPSQAVDSAARILQEQ
LQPFISSDVSYKKSQVSSSSGAKDLGYDPVLLRKVDEMELSVRSHNCLKNENITYIGDLVQKTEGEMLRTANFGRKSLNE
IKAVLTNFGLSLGMNVLNWPPKDIDELAKQHTDED

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=334, Percent_Identity=46.1077844311377, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000644
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 39070; Mature: 39070

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYNNDVSLCSNLDKLIKPSSVKVISDDSSEKSDIVLEPLESGFALTLGNALRRVMLSSL
CEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHC
KGSAVYGIKIEGVTHEFTSIQGVREDVTDIVLNMGMLRCKLNGASNKCLNLNAKGPCQVL
CCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCHHHHH
AGMIETDDQCSIVNKDLLICTLGQNVELNMTIYVASGKGYLPVTKYKENELLKLMSEQDL
HHHHCCCCCCEEECCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCC
IGFIPVNALYSPVNRVSYRVENSRVGQVTDKDKLILSIETDGTISPSQAVDSAARILQEQ
EEEEEHHHHHCCHHHHEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
LQPFISSDVSYKKSQVSSSSGAKDLGYDPVLLRKVDEMELSVRSHNCLKNENITYIGDLV
HHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
QKTEGEMLRTANFGRKSLNEIKAVLTNFGLSLGMNVLNWPPKDIDELAKQHTDED
HHHCCCEEHHHCCCHHHHHHHHHHHHHHCHHHCCHHHCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MYYNNDVSLCSNLDKLIKPSSVKVISDDSSEKSDIVLEPLESGFALTLGNALRRVMLSSL
CEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHC
KGSAVYGIKIEGVTHEFTSIQGVREDVTDIVLNMGMLRCKLNGASNKCLNLNAKGPCQVL
CCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCHHHHH
AGMIETDDQCSIVNKDLLICTLGQNVELNMTIYVASGKGYLPVTKYKENELLKLMSEQDL
HHHHCCCCCCEEECCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCC
IGFIPVNALYSPVNRVSYRVENSRVGQVTDKDKLILSIETDGTISPSQAVDSAARILQEQ
EEEEEHHHHHCCHHHHEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
LQPFISSDVSYKKSQVSSSSGAKDLGYDPVLLRKVDEMELSVRSHNCLKNENITYIGDLV
HHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
QKTEGEMLRTANFGRKSLNEIKAVLTNFGLSLGMNVLNWPPKDIDELAKQHTDED
HHHCCCEEHHHCCCHHHHHHHHHHHHHHCHHHCCHHHCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA