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The map label for this gene is lpd [H]

Identifier: 225630261

GI number: 225630261

Start: 516270

End: 517643

Strand: Reverse

Name: lpd [H]

Synonym: WRi_004760

Alternate gene names: 225630261

Gene position: 517643-516270 (Counterclockwise)

Preceding gene: 225630263

Following gene: 225630259

Centisome position: 35.8

GC content: 36.97

Gene sequence:

>1374_bases
ATGAATGAGTATGATATTACGGTTATAGGTAGCGGTCCTGGTGGTTATATAGCGGCAATTAGGGCGGCACAGCTTGGGTT
TAAAACTGCAATTGTAGAAAAAGAAAAAAATTTAGGCGGTATATGCTTAAATTGGGGATGTATACCAACAAAATCACTGC
TTAGAGCATCTGAGGTTTATAGGCTAATAAAAAGATCAAAAGAGTTTGGTATAGAAGTAAAGGGAGCGAATTTTGATATA
CAATCAATAGTGAAATATTCAAGAAACGTTGTTGATAAATTGTCAAGCGGTGTTGCTTATTTGATGAAGAAGAATAACAT
CAAAGTTCATCAGGGCTTTGGTAAACTTGCAGGCAATGGTACTATAAAAGTTGCTGGCGATAAGGAAGAACAAGAAATTG
TTTCCAAGCATATCATTTTAGCAACAGGCGTGAGGGCACGGAATCTGCCTGGAATAGAGGCAGATGGAGATTTAATATGG
AATGCGCAGCACGCTATGACTCCAGAGAGATTACCAAAATCGCTACTAATTATAGGGTCTGGTGCAATTGGAATAGAATT
TGCAAGTTTTTATAGTACTTTGGGTGTTGATGTAACAATCATAGAGATAAAGAGCACTATTTTGCCGCTGGAGGATAAGG
ACATTTCAGATTTAGCACAAGAAATATTCACAAAACAGGGGATAGAAATATATACAAGCAGTAGCGTAAAAGCTCTTACT
AAAAGTAAAGACTCTGCTCAAGTGCTACTAAGTAGTGGTGAGAGCAAAGAATTTGAGAGGGTGATTGTTGCGGTTGGAAT
TCAAGCAAATATTGAAAATATAGGTTTAGAAAATACAAAAATTAAATTAAGCCCTTCTGGCTTTATTGAAACGAATGAAT
GGTATGAAACTAGTGAATCAAATGTGTATGCAATAGGTGATGTAGCTGGCCCACCATGTTTAGCACATAAAGCGAGCCAT
GAAGCCGTGATCTGCATTGAAAAGATTGCTGGTAAAAATGCTCATGCGTTAAAAAAAGAATGCATACCAAATTGCACTTA
TTCTCATCCACAAATAGCGAGCATCGGCCTTACTGAGGAACAGGCAATAAAAGGTGGGTATGATATAAAAATAGGAAAAT
TTCACTCTAATTTTAATGGTAAGTCTGTTGCACTCAGTGAAACTGAAGGGTTAGTGAAAACGATTATAGATAAAAAAACC
GGAGAAATTCTGGGTAGCCACATGATAGGTGCAGAAGTAACGGAGTTAATTAGCAATTTTGCTCTTGTAAAGCAACTAGA
AGGAACAGACTTCGACATAAAATCTACGATTTTTCCTCATCCAACCATTTCAGAGATGATACATGAATCAGTGCTTGCTG
CAGATGTTAAATAG

Upstream 100 bases:

>100_bases
TGTCAAGTAAGTTTTTCGTTTCTATGTTGTCAGCTACCTGAATATTGATCATACGGGTTCCATCTGTTATATATAATCTT
TTTGAATACGAAGAAAACTG

Downstream 100 bases:

>100_bases
TTGAACTAACGTTCCATCGTTTTAGAGTTGTCAATGTCTTGAGTAGTAGTTTGATTAGGCACTTTAGTTTCAATAGATTG
ATTACCACCAGCATCAGAAT

Product: Dihydrolipoamide dehydrogenase E3 component

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNEYDITVIGSGPGGYIAAIRAAQLGFKTAIVEKEKNLGGICLNWGCIPTKSLLRASEVYRLIKRSKEFGIEVKGANFDI
QSIVKYSRNVVDKLSSGVAYLMKKNNIKVHQGFGKLAGNGTIKVAGDKEEQEIVSKHIILATGVRARNLPGIEADGDLIW
NAQHAMTPERLPKSLLIIGSGAIGIEFASFYSTLGVDVTIIEIKSTILPLEDKDISDLAQEIFTKQGIEIYTSSSVKALT
KSKDSAQVLLSSGESKEFERVIVAVGIQANIENIGLENTKIKLSPSGFIETNEWYETSESNVYAIGDVAGPPCLAHKASH
EAVICIEKIAGKNAHALKKECIPNCTYSHPQIASIGLTEEQAIKGGYDIKIGKFHSNFNGKSVALSETEGLVKTIIDKKT
GEILGSHMIGAEVTELISNFALVKQLEGTDFDIKSTIFPHPTISEMIHESVLAADVK

Sequences:

>Translated_457_residues
MNEYDITVIGSGPGGYIAAIRAAQLGFKTAIVEKEKNLGGICLNWGCIPTKSLLRASEVYRLIKRSKEFGIEVKGANFDI
QSIVKYSRNVVDKLSSGVAYLMKKNNIKVHQGFGKLAGNGTIKVAGDKEEQEIVSKHIILATGVRARNLPGIEADGDLIW
NAQHAMTPERLPKSLLIIGSGAIGIEFASFYSTLGVDVTIIEIKSTILPLEDKDISDLAQEIFTKQGIEIYTSSSVKALT
KSKDSAQVLLSSGESKEFERVIVAVGIQANIENIGLENTKIKLSPSGFIETNEWYETSESNVYAIGDVAGPPCLAHKASH
EAVICIEKIAGKNAHALKKECIPNCTYSHPQIASIGLTEEQAIKGGYDIKIGKFHSNFNGKSVALSETEGLVKTIIDKKT
GEILGSHMIGAEVTELISNFALVKQLEGTDFDIKSTIFPHPTISEMIHESVLAADVK
>Mature_457_residues
MNEYDITVIGSGPGGYIAAIRAAQLGFKTAIVEKEKNLGGICLNWGCIPTKSLLRASEVYRLIKRSKEFGIEVKGANFDI
QSIVKYSRNVVDKLSSGVAYLMKKNNIKVHQGFGKLAGNGTIKVAGDKEEQEIVSKHIILATGVRARNLPGIEADGDLIW
NAQHAMTPERLPKSLLIIGSGAIGIEFASFYSTLGVDVTIIEIKSTILPLEDKDISDLAQEIFTKQGIEIYTSSSVKALT
KSKDSAQVLLSSGESKEFERVIVAVGIQANIENIGLENTKIKLSPSGFIETNEWYETSESNVYAIGDVAGPPCLAHKASH
EAVICIEKIAGKNAHALKKECIPNCTYSHPQIASIGLTEEQAIKGGYDIKIGKFHSNFNGKSVALSETEGLVKTIIDKKT
GEILGSHMIGAEVTELISNFALVKQLEGTDFDIKSTIFPHPTISEMIHESVLAADVK

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=463, Percent_Identity=37.3650107991361, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=27.4468085106383, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI33519430, Length=456, Percent_Identity=23.6842105263158, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI33519428, Length=456, Percent_Identity=23.6842105263158, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI33519426, Length=456, Percent_Identity=23.6842105263158, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI148277065, Length=456, Percent_Identity=23.6842105263158, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI148277071, Length=456, Percent_Identity=23.6842105263158, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI22035672, Length=477, Percent_Identity=24.7379454926625, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI291045266, Length=427, Percent_Identity=24.824355971897, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI291045268, Length=283, Percent_Identity=26.1484098939929, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786307, Length=459, Percent_Identity=33.5511982570806, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI87082354, Length=451, Percent_Identity=27.0509977827051, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=27.9816513761468, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI87081717, Length=462, Percent_Identity=27.7056277056277, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1788892, Length=229, Percent_Identity=27.9475982532751, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=460, Percent_Identity=34.5652173913044, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=24.8407643312102, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=466, Percent_Identity=25.7510729613734, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=466, Percent_Identity=25.7510729613734, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=487, Percent_Identity=23.6139630390144, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=467, Percent_Identity=35.3319057815846, Blast_Score=262, Evalue=9e-71,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=29.8097251585624, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6325166, Length=462, Percent_Identity=28.1385281385281, Blast_Score=152, Evalue=9e-38,
Organism=Drosophila melanogaster, GI21358499, Length=462, Percent_Identity=37.8787878787879, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI17737741, Length=471, Percent_Identity=23.3545647558386, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640549, Length=468, Percent_Identity=23.2905982905983, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24640553, Length=468, Percent_Identity=23.2905982905983, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24640551, Length=470, Percent_Identity=23.4042553191489, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49373; Mature: 49373

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEYDITVIGSGPGGYIAAIRAAQLGFKTAIVEKEKNLGGICLNWGCIPTKSLLRASEVY
CCCEEEEEEECCCCCHHHHHHHHHHCHHHHEEECCCCCCCEEEECCCCCHHHHHHHHHHH
RLIKRSKEFGIEVKGANFDIQSIVKYSRNVVDKLSSGVAYLMKKNNIKVHQGFGKLAGNG
HHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCHHCCCC
TIKVAGDKEEQEIVSKHIILATGVRARNLPGIEADGDLIWNAQHAMTPERLPKSLLIIGS
EEEEECCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCEEECCHHCCCHHHCCCEEEEEEC
GAIGIEFASFYSTLGVDVTIIEIKSTILPLEDKDISDLAQEIFTKQGIEIYTSSSVKALT
CCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHH
KSKDSAQVLLSSGESKEFERVIVAVGIQANIENIGLENTKIKLSPSGFIETNEWYETSES
CCCCCHHHEECCCCCHHHEEEEEEEECEECHHHCCCCCCEEEECCCCCEECCCCCCCCCC
NVYAIGDVAGPPCLAHKASHEAVICIEKIAGKNAHALKKECIPNCTYSHPQIASIGLTEE
CEEEEECCCCCCHHHCCCCCCEEEEEEHHCCCCHHHHHHHHCCCCCCCCCCEEEECCCHH
QAIKGGYDIKIGKFHSNFNGKSVALSETEGLVKTIIDKKTGEILGSHMIGAEVTELISNF
HHHCCCCEEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVKQLEGTDFDIKSTIFPHPTISEMIHESVLAADVK
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEYDITVIGSGPGGYIAAIRAAQLGFKTAIVEKEKNLGGICLNWGCIPTKSLLRASEVY
CCCEEEEEEECCCCCHHHHHHHHHHCHHHHEEECCCCCCCEEEECCCCCHHHHHHHHHHH
RLIKRSKEFGIEVKGANFDIQSIVKYSRNVVDKLSSGVAYLMKKNNIKVHQGFGKLAGNG
HHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCHHCCCC
TIKVAGDKEEQEIVSKHIILATGVRARNLPGIEADGDLIWNAQHAMTPERLPKSLLIIGS
EEEEECCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCEEECCHHCCCHHHCCCEEEEEEC
GAIGIEFASFYSTLGVDVTIIEIKSTILPLEDKDISDLAQEIFTKQGIEIYTSSSVKALT
CCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHH
KSKDSAQVLLSSGESKEFERVIVAVGIQANIENIGLENTKIKLSPSGFIETNEWYETSES
CCCCCHHHEECCCCCHHHEEEEEEEECEECHHHCCCCCCEEEECCCCCEECCCCCCCCCC
NVYAIGDVAGPPCLAHKASHEAVICIEKIAGKNAHALKKECIPNCTYSHPQIASIGLTEE
CEEEEECCCCCCHHHCCCCCCEEEEEEHHCCCCHHHHHHHHCCCCCCCCCCEEEECCCHH
QAIKGGYDIKIGKFHSNFNGKSVALSETEGLVKTIIDKKTGEILGSHMIGAEVTELISNF
HHHCCCCEEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVKQLEGTDFDIKSTIFPHPTISEMIHESVLAADVK
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]