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The map label for this gene is murG

Identifier: 225630259

GI number: 225630259

Start: 514771

End: 515802

Strand: Reverse

Name: murG

Synonym: WRi_004740

Alternate gene names: 225630259

Gene position: 515802-514771 (Counterclockwise)

Preceding gene: 225630261

Following gene: 225630256

Centisome position: 35.67

GC content: 33.82

Gene sequence:

>1032_bases
ATGGATATTATTTTAGCAACAGGTGGCACAGGTGGGCATATCTTTCCGGCCATAGCTTTAGCAAAAGCACTAAAGACACA
AGGATACAATTGCATATTATTCACTGATAAAAAAACAAATAAAAATACCGACATAGAAAGCTACACCTTACCATTACGTA
GACCAAGCAGCAACAAATTTAAGTTTTTCCTTTTCTTGATATACAGCTCTATGTTAGCACTGTATCAAGTAAGAAAATTA
AAACCAAAATCGGTCATTGGTTTTGGTAGCTATGCTTCTTTTCCAACTCTTCTTGCAGCAAGAGTTCTTTCTATACCTAT
AATTTTACATGAACAAAACACAGTTTTAGGGAGAGTAAATAGATTCTTTTTCAAGAGTGCAAAATTAATTGCAACCAGCT
TTCCGGAGACTAAATATGCAGAGGGCAATAAATGTATTTTTACAGGAAATTTTGTTGATATAAAAGCACAGAGCCATTCT
AGCACTGAGAAAATCCTAAATATATTGGTCATAGCAGGCAGCCAAGGTGCAAATTTTTTTGATGATGTAGTAAGCAGCGT
AATTTGTGATTTACCTATTAAAATGAAAAAGAAAATTAGAGTGACGCAGCAATGTACGAAGAAAAATGTAAACAAGGTCA
AAAGTCTATACAAAAGTGAAAAGATCGATTGTGAATTGAGTGAATTTTTTGATGATATGGAGAATAGACTGGCCAATGCT
CACTTGGTAATTAGCAGAGCAGGAGCAACTTCAATAGCAGAGATCACTCTTGCTAGGCGTTCTGCTATATATATTCCTTA
CCCTTACTCAAAAGATAATCACCAATTTTATAACGCAAAATATATTGAAGACTCGAGAGCAGCTATAATAGTTAAGCAGA
ATAGTGAAGCAAAAAAAAATCTAACAGAGGTACTATTTGATCTATTAAATAATTCTCAAAAATTGCGTGATATGACCAAT
AGTACAGAAAAAACAGGGATAAAGAATGGGACTACTGAGTTTGTTAAGGTGATTGTTCACAGGTTTAGTTGA

Upstream 100 bases:

>100_bases
TACTTTTAGGCATTTTTAACTCAACTATCATAACGCTTAATAAAATTCTACGTGCAAATTGTTAAATAACAGTTAACAAT
CTAGGATTTATTATTTACAG

Downstream 100 bases:

>100_bases
TGGCTGCTTGCTTTTTCTTCAAACACGTCCCAAAGATCCTTTTCTAGCGATATATTATACAATTTATTGATATAAACTAC
TCCCGTGGGTGAAGTTGTGT

Product: UDP-N-acetylglucosamine--N-acetylmuramyl- (pentape ptide) pyrophosphoryl-undecaprenol N-acetylglucosamine

Products: NA

Alternate protein names: Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MDIILATGGTGGHIFPAIALAKALKTQGYNCILFTDKKTNKNTDIESYTLPLRRPSSNKFKFFLFLIYSSMLALYQVRKL
KPKSVIGFGSYASFPTLLAARVLSIPIILHEQNTVLGRVNRFFFKSAKLIATSFPETKYAEGNKCIFTGNFVDIKAQSHS
STEKILNILVIAGSQGANFFDDVVSSVICDLPIKMKKKIRVTQQCTKKNVNKVKSLYKSEKIDCELSEFFDDMENRLANA
HLVISRAGATSIAEITLARRSAIYIPYPYSKDNHQFYNAKYIEDSRAAIIVKQNSEAKKNLTEVLFDLLNNSQKLRDMTN
STEKTGIKNGTTEFVKVIVHRFS

Sequences:

>Translated_343_residues
MDIILATGGTGGHIFPAIALAKALKTQGYNCILFTDKKTNKNTDIESYTLPLRRPSSNKFKFFLFLIYSSMLALYQVRKL
KPKSVIGFGSYASFPTLLAARVLSIPIILHEQNTVLGRVNRFFFKSAKLIATSFPETKYAEGNKCIFTGNFVDIKAQSHS
STEKILNILVIAGSQGANFFDDVVSSVICDLPIKMKKKIRVTQQCTKKNVNKVKSLYKSEKIDCELSEFFDDMENRLANA
HLVISRAGATSIAEITLARRSAIYIPYPYSKDNHQFYNAKYIEDSRAAIIVKQNSEAKKNLTEVLFDLLNNSQKLRDMTN
STEKTGIKNGTTEFVKVIVHRFS
>Mature_343_residues
MDIILATGGTGGHIFPAIALAKALKTQGYNCILFTDKKTNKNTDIESYTLPLRRPSSNKFKFFLFLIYSSMLALYQVRKL
KPKSVIGFGSYASFPTLLAARVLSIPIILHEQNTVLGRVNRFFFKSAKLIATSFPETKYAEGNKCIFTGNFVDIKAQSHS
STEKILNILVIAGSQGANFFDDVVSSVICDLPIKMKKKIRVTQQCTKKNVNKVKSLYKSEKIDCELSEFFDDMENRLANA
HLVISRAGATSIAEITLARRSAIYIPYPYSKDNHQFYNAKYIEDSRAAIIVKQNSEAKKNLTEVLFDLLNNSQKLRDMTN
STEKTGIKNGTTEFVKVIVHRFS

Specific function: Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II)

COG id: COG0707

COG function: function code M; UDP-N-acetylglucosamine:LPS N-acetylglucosamine transferase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 28 family. MurG subfamily

Homologues:

Organism=Escherichia coli, GI1786278, Length=304, Percent_Identity=30.2631578947368, Blast_Score=140, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURG_WOLPM (Q73I55)

Other databases:

- EMBL:   AE017196
- RefSeq:   NP_966123.1
- ProteinModelPortal:   Q73I55
- SMR:   Q73I55
- EnsemblBacteria:   EBWOLT00000004120
- GeneID:   2738621
- GenomeReviews:   AE017196_GR
- KEGG:   wol:WD0323
- TIGR:   WD_0323
- GeneTree:   EBGT00050000030899
- HOGENOM:   HBG617076
- OMA:   IGFGGYP
- ProtClustDB:   CLSK740297
- BioCyc:   WPIP955:WD_0323-MONOMER
- HAMAP:   MF_00033
- InterPro:   IPR006009
- InterPro:   IPR004276
- InterPro:   IPR007235

Pfam domain/function: PF04101 Glyco_tran_28_C; PF03033 Glyco_transf_28

EC number: =2.4.1.227

Molecular weight: Translated: 38533; Mature: 38533

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIILATGGTGGHIFPAIALAKALKTQGYNCILFTDKKTNKNTDIESYTLPLRRPSSNKF
CEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCH
KFFLFLIYSSMLALYQVRKLKPKSVIGFGSYASFPTLLAARVLSIPIILHEQNTVLGRVN
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCEEEEECCCHHHHHHH
RFFFKSAKLIATSFPETKYAEGNKCIFTGNFVDIKAQSHSSTEKILNILVIAGSQGANFF
HHHHHHHHHEEECCCCCCCCCCCEEEEECCEEEEEECCCCHHHHEEEEEEEECCCCCCHH
DDVVSSVICDLPIKMKKKIRVTQQCTKKNVNKVKSLYKSEKIDCELSEFFDDMENRLANA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCE
HLVISRAGATSIAEITLARRSAIYIPYPYSKDNHQFYNAKYIEDSRAAIIVKQNSEAKKN
EEEEEECCCCHHHHHEEECCCEEEEECCCCCCCCEEEEEEEEECCCEEEEEECCCHHHHH
LTEVLFDLLNNSQKLRDMTNSTEKTGIKNGTTEFVKVIVHRFS
HHHHHHHHHCCCHHHHHHHCCHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDIILATGGTGGHIFPAIALAKALKTQGYNCILFTDKKTNKNTDIESYTLPLRRPSSNKF
CEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCH
KFFLFLIYSSMLALYQVRKLKPKSVIGFGSYASFPTLLAARVLSIPIILHEQNTVLGRVN
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCEEEEECCCHHHHHHH
RFFFKSAKLIATSFPETKYAEGNKCIFTGNFVDIKAQSHSSTEKILNILVIAGSQGANFF
HHHHHHHHHEEECCCCCCCCCCCEEEEECCEEEEEECCCCHHHHEEEEEEEECCCCCCHH
DDVVSSVICDLPIKMKKKIRVTQQCTKKNVNKVKSLYKSEKIDCELSEFFDDMENRLANA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCE
HLVISRAGATSIAEITLARRSAIYIPYPYSKDNHQFYNAKYIEDSRAAIIVKQNSEAKKN
EEEEEECCCCHHHHHEEECCCEEEEECCCCCCCCEEEEEEEEECCCEEEEEECCCHHHHH
LTEVLFDLLNNSQKLRDMTNSTEKTGIKNGTTEFVKVIVHRFS
HHHHHHHHHCCCHHHHHHHCCHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA