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The map label for this gene is gshb [H]

Identifier: 225630258

GI number: 225630258

Start: 513857

End: 514786

Strand: Direct

Name: gshb [H]

Synonym: WRi_004730

Alternate gene names: 225630258

Gene position: 513857-514786 (Clockwise)

Preceding gene: 225630257

Following gene: 225630260

Centisome position: 35.54

GC content: 33.76

Gene sequence:

>930_bases
ATGAAAGTTGCCTTTCAGATGGATGAAAATATAAATTTTGAAACTGACACTACATTCGTGCTAATAAAGGAAGCACAAAG
GAGGAAGCACGAAGTTTTTGTCTATGCTCCTAACAATTTAGCACTAAAGCTAAACCATCCAATTGCTCTGGCTCAGAAAG
TCAGCGTTGATGATTTTAGTTTTATCTCTAAAGAGGATGTAGCAATCAACTTGAATGAAATGGACATCATATTTATCAGA
CAGGATCCACCCTTTGATATGCGTTATATTACAACAACATATATCTTAGAAAAAACCAGCGCATTGGTAATTAACAATCC
AACAGAAATAAGAAATTGTCCTGAAAAATTGATAACTTCATTATTTCCAGAGCTAATTCCGCCAACTTTGATTACTGAAA
ATATATCAATGATTAGAGATTTCTACTACAACTATCAAGATATTATTCTGAAGCCATTGTATAGTTATGGAGGAAACGAT
GTGATAAGAATACAAGATGAGAATAGTATTCAAGTAGTAGTGGATCTCATGATAGCAAAATACGAATGCCCTGTGATTGC
ACAATCATTTTGTAAAAACATAGATAAAGATAAAAGAATATTGCTACTTTATGGCCAACCAATTGGTGTAATGAAACGAA
TTCCAAAAATCAGTGGAGAGATCAGAACAAACATGCGACTTGGAGCAAGTTTTGAGCCCGCTCAAATGAACGATAGAAAC
AATGAAATATGTAACAAAATTGGTCCTGAATTAAAGAAAAGAGGGCTAATATTTGTTGGTATTGATATTATAGATGACTT
CCTGCTCGAAATTAACACAACTTCACCCACGGGAGTAGTTTATATCAATAAATTGTATAATATATCGCTAGAAAAGGATC
TTTGGGACGTGTTTGAAGAAAAAGCAAGCAGCCATCAACTAAACCTGTGA

Upstream 100 bases:

>100_bases
AAGTTATGTACGATATAGGTACAGTAAGTTACGCCATTAGGCAAAAATCGCTTTCAAATTATAACATTTGTACAATTGTG
ACAATAGGTAGAGAACATAA

Downstream 100 bases:

>100_bases
ACAATCACCTTAACAAACTCAGTAGTCCCATTCTTTATCCCTGTTTTTTCTGTACTATTGGTCATATCACGCAATTTTTG
AGAATTATTTAATAGATCAA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKVAFQMDENINFETDTTFVLIKEAQRRKHEVFVYAPNNLALKLNHPIALAQKVSVDDFSFISKEDVAINLNEMDIIFIR
QDPPFDMRYITTTYILEKTSALVINNPTEIRNCPEKLITSLFPELIPPTLITENISMIRDFYYNYQDIILKPLYSYGGND
VIRIQDENSIQVVVDLMIAKYECPVIAQSFCKNIDKDKRILLLYGQPIGVMKRIPKISGEIRTNMRLGASFEPAQMNDRN
NEICNKIGPELKKRGLIFVGIDIIDDFLLEINTTSPTGVVYINKLYNISLEKDLWDVFEEKASSHQLNL

Sequences:

>Translated_309_residues
MKVAFQMDENINFETDTTFVLIKEAQRRKHEVFVYAPNNLALKLNHPIALAQKVSVDDFSFISKEDVAINLNEMDIIFIR
QDPPFDMRYITTTYILEKTSALVINNPTEIRNCPEKLITSLFPELIPPTLITENISMIRDFYYNYQDIILKPLYSYGGND
VIRIQDENSIQVVVDLMIAKYECPVIAQSFCKNIDKDKRILLLYGQPIGVMKRIPKISGEIRTNMRLGASFEPAQMNDRN
NEICNKIGPELKKRGLIFVGIDIIDDFLLEINTTSPTGVVYINKLYNISLEKDLWDVFEEKASSHQLNL
>Mature_309_residues
MKVAFQMDENINFETDTTFVLIKEAQRRKHEVFVYAPNNLALKLNHPIALAQKVSVDDFSFISKEDVAINLNEMDIIFIR
QDPPFDMRYITTTYILEKTSALVINNPTEIRNCPEKLITSLFPELIPPTLITENISMIRDFYYNYQDIILKPLYSYGGND
VIRIQDENSIQVVVDLMIAKYECPVIAQSFCKNIDKDKRILLLYGQPIGVMKRIPKISGEIRTNMRLGASFEPAQMNDRN
NEICNKIGPELKKRGLIFVGIDIIDDFLLEINTTSPTGVVYINKLYNISLEKDLWDVFEEKASSHQLNL

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=314, Percent_Identity=36.3057324840764, Blast_Score=200, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35611; Mature: 35611

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAFQMDENINFETDTTFVLIKEAQRRKHEVFVYAPNNLALKLNHPIALAQKVSVDDFS
CEEEEEECCCCCCCCCCEEEEEECHHCCCCEEEEEECCCEEEEECCCEEEEEECCCCCHH
FISKEDVAINLNEMDIIFIRQDPPFDMRYITTTYILEKTSALVINNPTEIRNCPEKLITS
CCCCCCEEEEECCEEEEEEECCCCCCEEEEEEEEEEECCCEEEEECCHHHHCCHHHHHHH
LFPELIPPTLITENISMIRDFYYNYQDIILKPLYSYGGNDVIRIQDENSIQVVVDLMIAK
HHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEHHHH
YECPVIAQSFCKNIDKDKRILLLYGQPIGVMKRIPKISGEIRTNMRLGASFEPAQMNDRN
CCCCHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCCCCCEEECEEECCCCCCCCCCCCC
NEICNKIGPELKKRGLIFVGIDIIDDFLLEINTTSPTGVVYINKLYNISLEKDLWDVFEE
HHHHHHCCHHHHHCCEEEEEHHHHCCCCEEEECCCCCCEEEEEEEEEEEEHHHHHHHHHH
KASSHQLNL
HHCCCCCCC
>Mature Secondary Structure
MKVAFQMDENINFETDTTFVLIKEAQRRKHEVFVYAPNNLALKLNHPIALAQKVSVDDFS
CEEEEEECCCCCCCCCCEEEEEECHHCCCCEEEEEECCCEEEEECCCEEEEEECCCCCHH
FISKEDVAINLNEMDIIFIRQDPPFDMRYITTTYILEKTSALVINNPTEIRNCPEKLITS
CCCCCCEEEEECCEEEEEEECCCCCCEEEEEEEEEEECCCEEEEECCHHHHCCHHHHHHH
LFPELIPPTLITENISMIRDFYYNYQDIILKPLYSYGGNDVIRIQDENSIQVVVDLMIAK
HHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEHHHH
YECPVIAQSFCKNIDKDKRILLLYGQPIGVMKRIPKISGEIRTNMRLGASFEPAQMNDRN
CCCCHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCCCCCEEECEEECCCCCCCCCCCCC
NEICNKIGPELKKRGLIFVGIDIIDDFLLEINTTSPTGVVYINKLYNISLEKDLWDVFEE
HHHHHHCCHHHHHCCEEEEEHHHHCCCCEEEECCCCCCEEEEEEEEEEEEHHHHHHHHHH
KASSHQLNL
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1540152 [H]