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The map label for this gene is tig [H]

Identifier: 225630256

GI number: 225630256

Start: 511485

End: 512822

Strand: Reverse

Name: tig [H]

Synonym: WRi_004700

Alternate gene names: 225630256

Gene position: 512822-511485 (Counterclockwise)

Preceding gene: 225630259

Following gene: 225630255

Centisome position: 35.47

GC content: 31.61

Gene sequence:

>1338_bases
ATGATGTCTAATAATATACCTCAAAATACAGTCGAAGTAAGTAGCGTATATACCTACAAAGAGCTTAGTATAGATAAACT
AAAGTATGAGTATGAAATCACAGTTGGTAGTGATTATATAAAACAAAAGGTAAATTCCAGGTTGCAAGAGATAGCAGAAA
ATGCAAAATCACCTGGATTTAGGGCTGGAAAGATGCCCTATGACCTTGTTGTTGCAAATTATAAAAACGAGGCTTTGGAA
TATGTGATAAATAATACAATTGATTATTGCTCAAGTGATTTGATGAAAAAAATTGAAGTCAAGTCTCACATTTATCCTAA
GGTTGATGTTATATCATTACCAGATCTGGGCAAAGAAAATGAGGAGGGCAATTTTGTATACAAGCTATCTTTTGAGTCGA
TGCCTGAAGTACCGATGATAGATCTTGACAAAATAAACTTAAAGAAAATTGAAGCAAAGATTGAAGAGGAAGATATAAAA
GAATTTATTGATTCTATCAAAACAAAGTTCCCCAATTTTGCCTCTGTTGATGATGCTTCTTATCAAGCGAAAGATGGGGA
TAAATTGATAATTGATTTTGAAGGGCGGATTAGGAATAAGCTCTTTCAAGGTGGAAGTAGCAAAAATTTTGCCGTTAATT
TAGGGTCTGGCACATTTATTAATGGTTTTGAAGATCAATTAACTGGTATGAAAAAGGGAGAAACAAAGAACTTTAAGTTG
AAATTTCCTGAAAATTACCAAGCCATTTCCCTTGCAGGACAGGAAGCTGATTTTTCTGTGCGAGTTAATGAGATTCAAAT
TGCCAAAGATTTTGAAAATGATGATGAAATAGCTAAGAGTATTGGGTTTAAAGATTATTCTTTGCTAATAAATCATGCAA
AAAAAATGATTGGTGATCAGTGCACCGAAATGAGAAATCTCTTAATTAAAAAGGAGCTGTTTGATTATTTGGATGCTAAC
TATAGTTTTGATTTACCCACAGACATAGTAAAACAAGAGCAACAAAGAATGGAAGGAGAATTGGGTGCTCAAAATGATTC
TCGTAAAGAAGCTGAAAAACGTGTAAAGCTTGCTATGTTATTTATGAAATTTAGTGCAGAACATAAAATATCATTAACTC
AAAATGATGTTTTGAGTGTTATTGTAAATCAATATGTCAGTAAAGATGTGCAATTTGACAGAGTACTTAAGCATTTTGAA
TCAAATAAACAATTTCAAGAATTAGTTAGAGGGCAAGCGCTTGAGTATAAAGTGACAGATTATATAATAGAAAAAGTTAG
TAAAGAAGAGCAGATTATTTCTGTGAAAGAATTAAAGGAATTATTTGATAATATTTAG

Upstream 100 bases:

>100_bases
TTTTGTTTGTGTAAAAACCTGTTCTTAAGGATTGTGTTTAACTTAAATTCTGGTAAAATGACTCTATTAAGATATGAATT
AAAATGTTAATAAATGAATT

Downstream 100 bases:

>100_bases
TAGGAAAAGGTATGACTCTTATACCAATTGTAGTTGAACAAACTAGTCGCGGTGAGCGCGCTTATGACATATATTCAAGA
CTAGTGAAAGAAAGAATAAT

Product: trigger factor, putative

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MMSNNIPQNTVEVSSVYTYKELSIDKLKYEYEITVGSDYIKQKVNSRLQEIAENAKSPGFRAGKMPYDLVVANYKNEALE
YVINNTIDYCSSDLMKKIEVKSHIYPKVDVISLPDLGKENEEGNFVYKLSFESMPEVPMIDLDKINLKKIEAKIEEEDIK
EFIDSIKTKFPNFASVDDASYQAKDGDKLIIDFEGRIRNKLFQGGSSKNFAVNLGSGTFINGFEDQLTGMKKGETKNFKL
KFPENYQAISLAGQEADFSVRVNEIQIAKDFENDDEIAKSIGFKDYSLLINHAKKMIGDQCTEMRNLLIKKELFDYLDAN
YSFDLPTDIVKQEQQRMEGELGAQNDSRKEAEKRVKLAMLFMKFSAEHKISLTQNDVLSVIVNQYVSKDVQFDRVLKHFE
SNKQFQELVRGQALEYKVTDYIIEKVSKEEQIISVKELKELFDNI

Sequences:

>Translated_445_residues
MMSNNIPQNTVEVSSVYTYKELSIDKLKYEYEITVGSDYIKQKVNSRLQEIAENAKSPGFRAGKMPYDLVVANYKNEALE
YVINNTIDYCSSDLMKKIEVKSHIYPKVDVISLPDLGKENEEGNFVYKLSFESMPEVPMIDLDKINLKKIEAKIEEEDIK
EFIDSIKTKFPNFASVDDASYQAKDGDKLIIDFEGRIRNKLFQGGSSKNFAVNLGSGTFINGFEDQLTGMKKGETKNFKL
KFPENYQAISLAGQEADFSVRVNEIQIAKDFENDDEIAKSIGFKDYSLLINHAKKMIGDQCTEMRNLLIKKELFDYLDAN
YSFDLPTDIVKQEQQRMEGELGAQNDSRKEAEKRVKLAMLFMKFSAEHKISLTQNDVLSVIVNQYVSKDVQFDRVLKHFE
SNKQFQELVRGQALEYKVTDYIIEKVSKEEQIISVKELKELFDNI
>Mature_445_residues
MMSNNIPQNTVEVSSVYTYKELSIDKLKYEYEITVGSDYIKQKVNSRLQEIAENAKSPGFRAGKMPYDLVVANYKNEALE
YVINNTIDYCSSDLMKKIEVKSHIYPKVDVISLPDLGKENEEGNFVYKLSFESMPEVPMIDLDKINLKKIEAKIEEEDIK
EFIDSIKTKFPNFASVDDASYQAKDGDKLIIDFEGRIRNKLFQGGSSKNFAVNLGSGTFINGFEDQLTGMKKGETKNFKL
KFPENYQAISLAGQEADFSVRVNEIQIAKDFENDDEIAKSIGFKDYSLLINHAKKMIGDQCTEMRNLLIKKELFDYLDAN
YSFDLPTDIVKQEQQRMEGELGAQNDSRKEAEKRVKLAMLFMKFSAEHKISLTQNDVLSVIVNQYVSKDVQFDRVLKHFE
SNKQFQELVRGQALEYKVTDYIIEKVSKEEQIISVKELKELFDNI

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=427, Percent_Identity=25.9953161592506, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 51170; Mature: 51170

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSNNIPQNTVEVSSVYTYKELSIDKLKYEYEITVGSDYIKQKVNSRLQEIAENAKSPGF
CCCCCCCCCCEEHHHEEEHHHCCHHHEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCC
RAGKMPYDLVVANYKNEALEYVINNTIDYCSSDLMKKIEVKSHIYPKVDVISLPDLGKEN
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
EEGNFVYKLSFESMPEVPMIDLDKINLKKIEAKIEEEDIKEFIDSIKTKFPNFASVDDAS
CCCCEEEEEECCCCCCCCCEECCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCC
YQAKDGDKLIIDFEGRIRNKLFQGGSSKNFAVNLGSGTFINGFEDQLTGMKKGETKNFKL
CCCCCCCEEEEEECCHHHHHHHCCCCCCCEEEECCCCCEECCHHHHHCCCCCCCCCCEEE
KFPENYQAISLAGQEADFSVRVNEIQIAKDFENDDEIAKSIGFKDYSLLINHAKKMIGDQ
ECCCCCCEEEECCCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHH
CTEMRNLLIKKELFDYLDANYSFDLPTDIVKQEQQRMEGELGAQNDSRKEAEKRVKLAML
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FMKFSAEHKISLTQNDVLSVIVNQYVSKDVQFDRVLKHFESNKQFQELVRGQALEYKVTD
HHHHCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHCCCCCEEHHHH
YIIEKVSKEEQIISVKELKELFDNI
HHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMSNNIPQNTVEVSSVYTYKELSIDKLKYEYEITVGSDYIKQKVNSRLQEIAENAKSPGF
CCCCCCCCCCEEHHHEEEHHHCCHHHEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCC
RAGKMPYDLVVANYKNEALEYVINNTIDYCSSDLMKKIEVKSHIYPKVDVISLPDLGKEN
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
EEGNFVYKLSFESMPEVPMIDLDKINLKKIEAKIEEEDIKEFIDSIKTKFPNFASVDDAS
CCCCEEEEEECCCCCCCCCEECCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCC
YQAKDGDKLIIDFEGRIRNKLFQGGSSKNFAVNLGSGTFINGFEDQLTGMKKGETKNFKL
CCCCCCCEEEEEECCHHHHHHHCCCCCCCEEEECCCCCEECCHHHHHCCCCCCCCCCEEE
KFPENYQAISLAGQEADFSVRVNEIQIAKDFENDDEIAKSIGFKDYSLLINHAKKMIGDQ
ECCCCCCEEEECCCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHH
CTEMRNLLIKKELFDYLDANYSFDLPTDIVKQEQQRMEGELGAQNDSRKEAEKRVKLAML
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FMKFSAEHKISLTQNDVLSVIVNQYVSKDVQFDRVLKHFESNKQFQELVRGQALEYKVTD
HHHHCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHCCCCCEEHHHH
YIIEKVSKEEQIISVKELKELFDNI
HHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA