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The map label for this gene is aspS [H]

Identifier: 225630146

GI number: 225630146

Start: 357584

End: 359386

Strand: Reverse

Name: aspS [H]

Synonym: WRi_003280

Alternate gene names: 225630146

Gene position: 359386-357584 (Counterclockwise)

Preceding gene: 225630149

Following gene: 225630145

Centisome position: 24.86

GC content: 38.77

Gene sequence:

>1803_bases
ATGAACTGCTATAAAACTCATACGTGCAATGAACTGAGGAAGAACGACGTAGAAAAGGAAGTTACCCTTTCTGGATGGTT
ATACCGTAAGCGTGATCACGGCAACCTAATCTTTGTTGATCTAAGAGACTTCTATGGAATCACTCAACTAGTATTTAATA
ATGACAAAGACTTTTTTGATGAGATATCAAATTTAAAATTGGAGAGTGTAATTACTGTTACAGGAATAGTTGAAGCTAGA
ACTGAAGATACGGTAAACAGCTCTATTTCTACCGGAGAAATCGAGGTGATAGTCAACAATTTGCGCGTTGAGTCGGAAGT
TGAGTTCCACTTTGATGAAGAAATAGCAAAAGAAGAAAGAAGTATATTGGCAAGCATTACCGGCGAGCAAGAATACCCAG
AAAACATGAGATTTAAATATCGATTTCTTGACTTAAGACGTGAAAAAGTCCGCAACAATATTATTTTGCGCTCACAAATT
ATTGCAGAGCTCCGCAAGCTCATGATAGAGCGAGGATTTTTAGAGATTCAAACTCCAATACTCACTGCTTCCTCTCCTGA
AGGAGCACGCGACTACCTAGTGCCAAGCAGGCTAAATCCTGGCAAGTTCTATGCATTGCCACAAGCTCCGCAGATTTTCA
AACAGTTGCTCATGGTTTCAGGGTTTGATAAATATTTCCAAATTGCACCGTGTTTTCGTGATGAAGACGCAAGGGCTGAC
CGTTCTCCGGGAGAGTTTTATCAGCTAGATCTTGAAATGTCTTTTGTAACTCAAGAAGACATCTTTCAGATTATTGAGTC
TACCTTATATAGAGTGTTTGCTAAATTTTCTCGTAAGTCTGTTGATAAAGATTTTCCACGTATTACATATAAAGAAGCAA
TGCTTAAATATGGTTCTGATAAGCCAGATCTGCGCAATCCACTATTAATCAGCGATGTTACAGAAATTTTTCGTGATTCA
GGGTTCAATATTTTCAAAAGCAATATTGAGCGTGGTATGGTAGTGAGAGCCATCCCTGCACCTAAAACAGCAGAGGAACC
ACGCAGCTTCTTTGATAAAAAGATAGAACATGCGCAAAAAGAATTCGGTGCTAAGGGTCTCGGATATATAACGTTTGATA
AAGATGGAACTGCGAAAGGCCCAATTGCTAAATTTCTTGATGAAAATAGGCTAAACCACATAAGAGAAGCAACAAACATA
GAGCCAGGAGATAGTGTATTTTTTGCTTCTGACAAAGAGAATGAGGCGGCAAACATTGCAGGAAAAGTACGCACTCTTTT
AGGGTCAGAACTCAGTCTTATAGATGATAATATCTTCAAGTTTTGTTGGATCATTGATTTTCCATATTTTGTATATGACG
ATAAAAGTAAAAAAATCGATTTCTTTCATAACCCATTCTCCATGCCACACGGTGGTCTGAAAGATTTAGAGGACAAAAAT
CCACTGGATATCCTTGCTTACCAATATGATCTTGTTTGCAATGGAATAGAACTGTCAAGTGGGGCGATTCGTAATAACAA
ACTGGATATCATGTATAAAGCTTTTGCCATTGCAGGTTATAGCCGGGGAGAAGTTGATACAAGATTCGGTGCACTTGTAC
GTGCATTCAGATTTGGTGTTCCTCCTCATGGTGGAATAGCACCAGGAGTTGATAGAATAGTTATGCTGCTTGCTGATGAG
CCAAATATTCGTGAAGTAATCTGTTTTCCTATGAACCAGCAAGGTGAAGATGTTCTAATGGGTGCTCCTTCCAAGGTGGA
GGATAAGCATTTACGTGAATTATCCTTGAAGGTTATTGAATGA

Upstream 100 bases:

>100_bases
ACTGCTCTATTTCGAAAATTGCGCAAATATCTGACGAACGGGCTTGTATGTAATAAGGTTTATTTCTATACTTCTTAAAG
AATTTAGAAGTAAAACAAAT

Downstream 100 bases:

>100_bases
AAAATTTTATAGCAAGGAGCACCGGTTAATGATATATTAGGTTATGTTAAATTGAAAGACCATGAATAATATCCAAAAAG
CAATACTATCGGGCATGATC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MNCYKTHTCNELRKNDVEKEVTLSGWLYRKRDHGNLIFVDLRDFYGITQLVFNNDKDFFDEISNLKLESVITVTGIVEAR
TEDTVNSSISTGEIEVIVNNLRVESEVEFHFDEEIAKEERSILASITGEQEYPENMRFKYRFLDLRREKVRNNIILRSQI
IAELRKLMIERGFLEIQTPILTASSPEGARDYLVPSRLNPGKFYALPQAPQIFKQLLMVSGFDKYFQIAPCFRDEDARAD
RSPGEFYQLDLEMSFVTQEDIFQIIESTLYRVFAKFSRKSVDKDFPRITYKEAMLKYGSDKPDLRNPLLISDVTEIFRDS
GFNIFKSNIERGMVVRAIPAPKTAEEPRSFFDKKIEHAQKEFGAKGLGYITFDKDGTAKGPIAKFLDENRLNHIREATNI
EPGDSVFFASDKENEAANIAGKVRTLLGSELSLIDDNIFKFCWIIDFPYFVYDDKSKKIDFFHNPFSMPHGGLKDLEDKN
PLDILAYQYDLVCNGIELSSGAIRNNKLDIMYKAFAIAGYSRGEVDTRFGALVRAFRFGVPPHGGIAPGVDRIVMLLADE
PNIREVICFPMNQQGEDVLMGAPSKVEDKHLRELSLKVIE

Sequences:

>Translated_600_residues
MNCYKTHTCNELRKNDVEKEVTLSGWLYRKRDHGNLIFVDLRDFYGITQLVFNNDKDFFDEISNLKLESVITVTGIVEAR
TEDTVNSSISTGEIEVIVNNLRVESEVEFHFDEEIAKEERSILASITGEQEYPENMRFKYRFLDLRREKVRNNIILRSQI
IAELRKLMIERGFLEIQTPILTASSPEGARDYLVPSRLNPGKFYALPQAPQIFKQLLMVSGFDKYFQIAPCFRDEDARAD
RSPGEFYQLDLEMSFVTQEDIFQIIESTLYRVFAKFSRKSVDKDFPRITYKEAMLKYGSDKPDLRNPLLISDVTEIFRDS
GFNIFKSNIERGMVVRAIPAPKTAEEPRSFFDKKIEHAQKEFGAKGLGYITFDKDGTAKGPIAKFLDENRLNHIREATNI
EPGDSVFFASDKENEAANIAGKVRTLLGSELSLIDDNIFKFCWIIDFPYFVYDDKSKKIDFFHNPFSMPHGGLKDLEDKN
PLDILAYQYDLVCNGIELSSGAIRNNKLDIMYKAFAIAGYSRGEVDTRFGALVRAFRFGVPPHGGIAPGVDRIVMLLADE
PNIREVICFPMNQQGEDVLMGAPSKVEDKHLRELSLKVIE
>Mature_600_residues
MNCYKTHTCNELRKNDVEKEVTLSGWLYRKRDHGNLIFVDLRDFYGITQLVFNNDKDFFDEISNLKLESVITVTGIVEAR
TEDTVNSSISTGEIEVIVNNLRVESEVEFHFDEEIAKEERSILASITGEQEYPENMRFKYRFLDLRREKVRNNIILRSQI
IAELRKLMIERGFLEIQTPILTASSPEGARDYLVPSRLNPGKFYALPQAPQIFKQLLMVSGFDKYFQIAPCFRDEDARAD
RSPGEFYQLDLEMSFVTQEDIFQIIESTLYRVFAKFSRKSVDKDFPRITYKEAMLKYGSDKPDLRNPLLISDVTEIFRDS
GFNIFKSNIERGMVVRAIPAPKTAEEPRSFFDKKIEHAQKEFGAKGLGYITFDKDGTAKGPIAKFLDENRLNHIREATNI
EPGDSVFFASDKENEAANIAGKVRTLLGSELSLIDDNIFKFCWIIDFPYFVYDDKSKKIDFFHNPFSMPHGGLKDLEDKN
PLDILAYQYDLVCNGIELSSGAIRNNKLDIMYKAFAIAGYSRGEVDTRFGALVRAFRFGVPPHGGIAPGVDRIVMLLADE
PNIREVICFPMNQQGEDVLMGAPSKVEDKHLRELSLKVIE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=610, Percent_Identity=33.7704918032787, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI45439306, Length=368, Percent_Identity=26.0869565217391, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI226491502, Length=263, Percent_Identity=25.4752851711027, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI5031815, Length=272, Percent_Identity=26.4705882352941, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI194272210, Length=272, Percent_Identity=26.4705882352941, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788173, Length=606, Percent_Identity=40.0990099009901, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1790571, Length=307, Percent_Identity=27.6872964169381, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1789256, Length=306, Percent_Identity=26.4705882352941, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787161, Length=304, Percent_Identity=25.3289473684211, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32566633, Length=312, Percent_Identity=38.4615384615385, Blast_Score=206, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17551876, Length=339, Percent_Identity=23.598820058997, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994340, Length=344, Percent_Identity=23.546511627907, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=344, Percent_Identity=23.546511627907, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=344, Percent_Identity=23.546511627907, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=673, Percent_Identity=32.2436849925706, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6323011, Length=310, Percent_Identity=29.6774193548387, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=261, Percent_Identity=24.5210727969349, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24584738, Length=622, Percent_Identity=34.7266881028939, Blast_Score=325, Evalue=5e-89,
Organism=Drosophila melanogaster, GI17136276, Length=318, Percent_Identity=24.8427672955975, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24640849, Length=266, Percent_Identity=24.0601503759398, Blast_Score=66, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24640851, Length=266, Percent_Identity=24.0601503759398, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 68768; Mature: 68768

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCYKTHTCNELRKNDVEKEVTLSGWLYRKRDHGNLIFVDLRDFYGITQLVFNNDKDFFD
CCCCCCCCHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEECHHHCCHHHHHCCCCHHHHH
EISNLKLESVITVTGIVEARTEDTVNSSISTGEIEVIVNNLRVESEVEFHFDEEIAKEER
HHHCCCHHHEEEEEEEEECCCCHHHCCCCCCCEEEEEEEEEEECCEEEECCCHHHHHHHH
SILASITGEQEYPENMRFKYRFLDLRREKVRNNIILRSQIIAELRKLMIERGFLEIQTPI
HHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCCEEEECCE
LTASSPEGARDYLVPSRLNPGKFYALPQAPQIFKQLLMVSGFDKYFQIAPCFRDEDARAD
EECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCC
RSPGEFYQLDLEMSFVTQEDIFQIIESTLYRVFAKFSRKSVDKDFPRITYKEAMLKYGSD
CCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
KPDLRNPLLISDVTEIFRDSGFNIFKSNIERGMVVRAIPAPKTAEEPRSFFDKKIEHAQK
CCCCCCCEEHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EFGAKGLGYITFDKDGTAKGPIAKFLDENRLNHIREATNIEPGDSVFFASDKENEAANIA
HHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHH
GKVRTLLGSELSLIDDNIFKFCWIIDFPYFVYDDKSKKIDFFHNPFSMPHGGLKDLEDKN
HHHHHHHCCCHHHHHHHHHHEEHEEECCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCC
PLDILAYQYDLVCNGIELSSGAIRNNKLDIMYKAFAIAGYSRGEVDTRFGALVRAFRFGV
CCEEEEEEEEEEECCEEECCCCCCCCCEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHCCC
PPHGGIAPGVDRIVMLLADEPNIREVICFPMNQQGEDVLMGAPSKVEDKHLRELSLKVIE
CCCCCCCCCHHHEEEEEECCCCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNCYKTHTCNELRKNDVEKEVTLSGWLYRKRDHGNLIFVDLRDFYGITQLVFNNDKDFFD
CCCCCCCCHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEECHHHCCHHHHHCCCCHHHHH
EISNLKLESVITVTGIVEARTEDTVNSSISTGEIEVIVNNLRVESEVEFHFDEEIAKEER
HHHCCCHHHEEEEEEEEECCCCHHHCCCCCCCEEEEEEEEEEECCEEEECCCHHHHHHHH
SILASITGEQEYPENMRFKYRFLDLRREKVRNNIILRSQIIAELRKLMIERGFLEIQTPI
HHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCCEEEECCE
LTASSPEGARDYLVPSRLNPGKFYALPQAPQIFKQLLMVSGFDKYFQIAPCFRDEDARAD
EECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCC
RSPGEFYQLDLEMSFVTQEDIFQIIESTLYRVFAKFSRKSVDKDFPRITYKEAMLKYGSD
CCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
KPDLRNPLLISDVTEIFRDSGFNIFKSNIERGMVVRAIPAPKTAEEPRSFFDKKIEHAQK
CCCCCCCEEHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EFGAKGLGYITFDKDGTAKGPIAKFLDENRLNHIREATNIEPGDSVFFASDKENEAANIA
HHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHH
GKVRTLLGSELSLIDDNIFKFCWIIDFPYFVYDDKSKKIDFFHNPFSMPHGGLKDLEDKN
HHHHHHHCCCHHHHHHHHHHEEHEEECCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCC
PLDILAYQYDLVCNGIELSSGAIRNNKLDIMYKAFAIAGYSRGEVDTRFGALVRAFRFGV
CCEEEEEEEEEEECCEEECCCCCCCCCEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHCCC
PPHGGIAPGVDRIVMLLADEPNIREVICFPMNQQGEDVLMGAPSKVEDKHLRELSLKVIE
CCCCCCCCCHHHEEEEEECCCCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA