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The map label for this gene is proP [H]

Identifier: 225630145

GI number: 225630145

Start: 356260

End: 357522

Strand: Reverse

Name: proP [H]

Synonym: WRi_003270

Alternate gene names: 225630145

Gene position: 357522-356260 (Counterclockwise)

Preceding gene: 225630146

Following gene: 225630142

Centisome position: 24.73

GC content: 33.17

Gene sequence:

>1263_bases
ATGAATAATATCCAAAAAGCAATACTATCGGGCATGATCTGTAACATGATTGTGTGGTATGAACTCACTCTTTTTGGAGT
ATTAACAGATACAATCATCAACGTTTTTTTCCCATCAGAAGATCATTATTTGAGTACACTCAAATTCCTTGGTGTTTTTG
CAATTGGTTTTGCGTTTAGACCAATTGGTGCAGTAATTTTTGGTTATATGGGAGATAAGTATGGCAGAAGAAAGACTTTA
CTTACTTCAGTAATATTAATTTCAATATCATCCACAATTATGGGAATTATACCTGGGTATAAAGAAATAGGGTTGCTTTC
ACCTGTATTGCTTGTGTTTTGTAGAATAGTGCAAGGAATAACTGCAGGAGGTGAAACAAGCATTAATACAGCATTTACGA
TAGAGCATTCAAAAGATAAAAAAAATCTAGGTTTCTTAGGAAGTATGAAGCCCTTTAGCGGAGCCCTTGGAACGCTCTTA
TGCATAACAATGATATTTATCTGCAAAAGAGTTACAGGTGAGAACTATGAAATATGGGGATGGAAGTTGCTTTTTTATTT
TTGCTTTTTTTTGGGGACAATAGGTTTTTTAACTAGGTACATATTAAAAGAAAGCTTAGCATATAAAACTAATAAGTACG
CTAATAATTTAGCACATTCACCATTGTTAGAACTAGTAAGAAACTATAAAAGACCGCTTATTATTTCAATCAATCTGGGA
ATTGCTCAGAATGCAATTGTCTACGCAGCAATAATGTTTTATAATATATCAATAAAAGAGCTAGTTATTTTATCCATTAG
TGTTAAAGATTTAATGAGGATAGTAATACAGGTTGTATTTGCAGCATCTGCGGTACTTTTTGCAATTTTATCTGATAAAG
TTGGAAGAAAACGCGTAATGATTCCTGTACTGATTATGCTATCTCTGATTGGTACATGTGTATTACCATTATTATCTTCC
GTTAACCACTATGTCGTAATAATAACTTACTTATTGATTACTATACCAGTAGGTGCATCTTTTGGAATATATGGTTCTGT
TATTTGTGAACTGTTTCCAACAAAAGTAAGATGCACTGGCGTAAGCTTAGCAAATAATATATCTGCAGGAATTTTTGGTG
GCCTTTCTCCATACATATGCATGTGGCTTATAGAAAAAACCGAAACAAAACTTGCAGCAGGCATTTATCTTACTGCCTGC
GCATTAATTAGCCTAATATCTGTGCTTCAAATTAAAGCCAAAGACAGAAAAGTTGATTGGTGA

Upstream 100 bases:

>100_bases
AAGCATTTACGTGAATTATCCTTGAAGGTTATTGAATGAAAAATTTTATAGCAAGGAGCACCGGTTAATGATATATTAGG
TTATGTTAAATTGAAAGACC

Downstream 100 bases:

>100_bases
AATCGTTTCAGTCGAAAAATAGCGCTTCAATGGATTTTTCGATGTGTTTAGTGATAGGAGCGGTGATAGTGATTTCTTTA
TTGTTTGGCAGTTTTAGAGA

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNNIQKAILSGMICNMIVWYELTLFGVLTDTIINVFFPSEDHYLSTLKFLGVFAIGFAFRPIGAVIFGYMGDKYGRRKTL
LTSVILISISSTIMGIIPGYKEIGLLSPVLLVFCRIVQGITAGGETSINTAFTIEHSKDKKNLGFLGSMKPFSGALGTLL
CITMIFICKRVTGENYEIWGWKLLFYFCFFLGTIGFLTRYILKESLAYKTNKYANNLAHSPLLELVRNYKRPLIISINLG
IAQNAIVYAAIMFYNISIKELVILSISVKDLMRIVIQVVFAASAVLFAILSDKVGRKRVMIPVLIMLSLIGTCVLPLLSS
VNHYVVIITYLLITIPVGASFGIYGSVICELFPTKVRCTGVSLANNISAGIFGGLSPYICMWLIEKTETKLAAGIYLTAC
ALISLISVLQIKAKDRKVDW

Sequences:

>Translated_420_residues
MNNIQKAILSGMICNMIVWYELTLFGVLTDTIINVFFPSEDHYLSTLKFLGVFAIGFAFRPIGAVIFGYMGDKYGRRKTL
LTSVILISISSTIMGIIPGYKEIGLLSPVLLVFCRIVQGITAGGETSINTAFTIEHSKDKKNLGFLGSMKPFSGALGTLL
CITMIFICKRVTGENYEIWGWKLLFYFCFFLGTIGFLTRYILKESLAYKTNKYANNLAHSPLLELVRNYKRPLIISINLG
IAQNAIVYAAIMFYNISIKELVILSISVKDLMRIVIQVVFAASAVLFAILSDKVGRKRVMIPVLIMLSLIGTCVLPLLSS
VNHYVVIITYLLITIPVGASFGIYGSVICELFPTKVRCTGVSLANNISAGIFGGLSPYICMWLIEKTETKLAAGIYLTAC
ALISLISVLQIKAKDRKVDW
>Mature_420_residues
MNNIQKAILSGMICNMIVWYELTLFGVLTDTIINVFFPSEDHYLSTLKFLGVFAIGFAFRPIGAVIFGYMGDKYGRRKTL
LTSVILISISSTIMGIIPGYKEIGLLSPVLLVFCRIVQGITAGGETSINTAFTIEHSKDKKNLGFLGSMKPFSGALGTLL
CITMIFICKRVTGENYEIWGWKLLFYFCFFLGTIGFLTRYILKESLAYKTNKYANNLAHSPLLELVRNYKRPLIISINLG
IAQNAIVYAAIMFYNISIKELVILSISVKDLMRIVIQVVFAASAVLFAILSDKVGRKRVMIPVLIMLSLIGTCVLPLLSS
VNHYVVIITYLLITIPVGASFGIYGSVICELFPTKVRCTGVSLANNISAGIFGGLSPYICMWLIEKTETKLAAGIYLTAC
ALISLISVLQIKAKDRKVDW

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=426, Percent_Identity=27.9342723004695, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789941, Length=418, Percent_Identity=29.1866028708134, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788292, Length=434, Percent_Identity=26.4976958525346, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788942, Length=419, Percent_Identity=26.7303102625298, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87082404, Length=325, Percent_Identity=24, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46432; Mature: 46432

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIQKAILSGMICNMIVWYELTLFGVLTDTIINVFFPSEDHYLSTLKFLGVFAIGFAFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
PIGAVIFGYMGDKYGRRKTLLTSVILISISSTIMGIIPGYKEIGLLSPVLLVFCRIVQGI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
TAGGETSINTAFTIEHSKDKKNLGFLGSMKPFSGALGTLLCITMIFICKRVTGENYEIWG
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHH
WKLLFYFCFFLGTIGFLTRYILKESLAYKTNKYANNLAHSPLLELVRNYKRPLIISINLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECC
IAQNAIVYAAIMFYNISIKELVILSISVKDLMRIVIQVVFAASAVLFAILSDKVGRKRVM
CHHHHHHHHHHHHHCCCHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IPVLIMLSLIGTCVLPLLSSVNHYVVIITYLLITIPVGASFGIYGSVICELFPTKVRCTG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEC
VSLANNISAGIFGGLSPYICMWLIEKTETKLAAGIYLTACALISLISVLQIKAKDRKVDW
CHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNNIQKAILSGMICNMIVWYELTLFGVLTDTIINVFFPSEDHYLSTLKFLGVFAIGFAFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
PIGAVIFGYMGDKYGRRKTLLTSVILISISSTIMGIIPGYKEIGLLSPVLLVFCRIVQGI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
TAGGETSINTAFTIEHSKDKKNLGFLGSMKPFSGALGTLLCITMIFICKRVTGENYEIWG
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHH
WKLLFYFCFFLGTIGFLTRYILKESLAYKTNKYANNLAHSPLLELVRNYKRPLIISINLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECC
IAQNAIVYAAIMFYNISIKELVILSISVKDLMRIVIQVVFAASAVLFAILSDKVGRKRVM
CHHHHHHHHHHHHHCCCHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IPVLIMLSLIGTCVLPLLSSVNHYVVIITYLLITIPVGASFGIYGSVICELFPTKVRCTG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEC
VSLANNISAGIFGGLSPYICMWLIEKTETKLAAGIYLTACALISLISVLQIKAKDRKVDW
CHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]