Click here to switch to the map view.

The map label for this gene is pheT [H]

Identifier: 225630137

GI number: 225630137

Start: 341543

End: 343882

Strand: Direct

Name: pheT [H]

Synonym: WRi_003190

Alternate gene names: 225630137

Gene position: 341543-343882 (Clockwise)

Preceding gene: 225630136

Following gene: 225630140

Centisome position: 23.62

GC content: 36.15

Gene sequence:

>2340_bases
ATGAAATTCACGTTATCTTGGTTACTGGAGTATTTAGAAACCAATGCTAGTTTAGAAGAAATTACCGATAAATTAACTCA
CATAGGGTTAGAAGTAGAAGATGTGATCGACAATACAAAATTAGCTGGTTTTATTGTTGCAGAAGTATTAGAGGTGATAC
AGCATCCAAATGCTGATAAATTAAAATTATGTAAAGTAGATGACGGAAGTAAAATTCTACAAATAGTTTGCGGAGCAAAC
AATGTTAGAGAAGGTATGAAAACTGTGCTTGCATCTCTTGGTAGCACATTGCCAGAAAGTGATTTCACAATCAAGCCTAC
AAAAATACGAGGAGTGCTAAGTGAAGGGATGCTCTGCTCTGCTTCTGAACTTGCGTTGGTTCAAGAGGAAAGTGAAGGAA
TAATCGAGCTTTCCGATGATTATAAAGTAGGGGATAAATTTTTCAATTGTGACCCTGTAATTGATATAAATGTTACCCCA
AACCGTGGAGATTGCTTAGGCATTTATGGAATAGCTCGCGACCTTGCTGCAACTGGAATTGGGACATTAAAGACTTTAAG
CATTCCACAACTTACCAGCTTCATAAATTCACCAATCGATGTTGAAGTTACTGACGGGGAAAGTTTTATTAGTGGAATAT
ACATCGCCAATGTAAAAAATCAAGAAAGCCCAAAATGGTTAAAAGATAGGCTGGAATCGATAGGAATGCGCTCCATTTCT
GCAATAGTTGATATCACGAACTATATTATGATATCTTTCAGCCGCCCAATGCACGCGTATGATGCAAAAAAAATAGAAGG
AGAGCTCACAGTACGCAAAGCAAACGACGGAGAAAAATTTGCTGGTTTGAACGGTAAAGAATACTTATTAAACAAAGACA
TAAGCGTTATTGCAGGTGATAAAAATATTCATGGAATTGCTGGAATTATAGGTGGTAAGTGCAGTGAATGTACTCTTGAA
ACTACTGATATCTTTTTAGAGTCTGCTTGGTTCGATCCCATCTCTATTACTAAATCTTCAAGGCAGCTCGGCATCTCCAC
AGACGCTAGTTACAGATTTGCACGTTCAGTTGATCCTGGATTTACTTTTGATGGACTCAACCTTGCAGCTAAAATGATTC
TGGACTTATGTGGTGGAGAAACCTCAAGCGTAGTGTCTGCTGGCAGCTTAGATAGTGTTGACACCAAGGTGAGCTTTGAT
TATCAGGATGTGAACAAGTTTGGAAGTGTGTCTGTATCGCCTGATGAGGTGCTTGATATCTTAACGAAACTAGGGTTTAG
TATTGATAAAAAAACCGAAGGCAATTGGAATGTACAAGTACCAAGCTGGAGACCGGACGTGACAATACCGGCTGACCTAA
TTGAAGAAGTAACAAGAATATACGGCTATGACAAAATAAAAGAAGAACCACTACCAAATAATGTTGAAGAAGTAGATAGT
ACATACGACAATTTGCGTATTTTGATGACAAACAGAGGGTTTCATGAAGTGCTAACCTGGTCGTTTATGAGTGAATCAAC
AGCCGAAAAATTTGGTTACTCGAATAAGTTATTTATCATCGATAATCCGTTTAACAATAACTTTAATATAATGAGACCAA
GTATTATGCCAAATTTATTGCAAGTTACTGCTGATAATATTGCCCACGGAATATCTGATCTTGCAATCTTCGAAATTGGG
CCGATTTACGATGGTGAAGCTCGGTCTAAACATGTCTTAAGTGGAATAAGAACAGGAAATAATTTACCGCGAAACCATTA
TAATACTGATAGGAAAGTAGATGTTTTTGACGCAAAGGCTGATTGCATAGCAGCTTTGGAGTTTTTTAACGTTAATTGTG
ATAATTTAACGATAGAGAAAGCAGAAAAAGAACATTACCACCCAGGAAAGTCAGGCACTCTCTCTTTTAAGAATAAAGTA
GCAGGTTATTTTGGAGAGTTGCATCCTAATATATTAGATCTTTTTGATATCAAGCAAAAAGTTGTAGGTTTTGAGGTAAT
ACTAGAAAATATTGAAAATTTACCTGTAAGTAGAAAGAAATTTATCGATTATAAATACCAAAGTGTAAAACGTGATTTTG
CATTTATAGTAAATAAGGATGTGGAAGTAGGTAACATAATCAATGTGATAAAAAAAAGCTCAGAGCTCATCACAGAAGTT
TTGATATTCGATGTATACCATGGAAATAATATAGAACCGAATAAGATGTCTATAGCGTTGTCAGTTACTTTTTGCTCTCC
AATCCACACTTTAACTGAAAAAGAGATCCAAAAAGAATCAAGTGCTATAGTTAATTTAGTATGTGAAAATACCGGAGGAA
CTTTGAGAACTTATCGTTGA

Upstream 100 bases:

>100_bases
TTCTACATCATAAACAATGATGTAGAAGTATTCCACTACAACAAGCTTTAAAAGCACTACATTTTATATTACAATAACTT
TTTTGTTTCTTGTTAGATCG

Downstream 100 bases:

>100_bases
GTCACAATCATAGTTTCCTGCCGTTGCTGTGTCACACTTACTTCATCCAATTTATTTCCAATATTGAATTCAATATCCTT
GAAAAATCTATCCCACATTC

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 779; Mature: 779

Protein sequence:

>779_residues
MKFTLSWLLEYLETNASLEEITDKLTHIGLEVEDVIDNTKLAGFIVAEVLEVIQHPNADKLKLCKVDDGSKILQIVCGAN
NVREGMKTVLASLGSTLPESDFTIKPTKIRGVLSEGMLCSASELALVQEESEGIIELSDDYKVGDKFFNCDPVIDINVTP
NRGDCLGIYGIARDLAATGIGTLKTLSIPQLTSFINSPIDVEVTDGESFISGIYIANVKNQESPKWLKDRLESIGMRSIS
AIVDITNYIMISFSRPMHAYDAKKIEGELTVRKANDGEKFAGLNGKEYLLNKDISVIAGDKNIHGIAGIIGGKCSECTLE
TTDIFLESAWFDPISITKSSRQLGISTDASYRFARSVDPGFTFDGLNLAAKMILDLCGGETSSVVSAGSLDSVDTKVSFD
YQDVNKFGSVSVSPDEVLDILTKLGFSIDKKTEGNWNVQVPSWRPDVTIPADLIEEVTRIYGYDKIKEEPLPNNVEEVDS
TYDNLRILMTNRGFHEVLTWSFMSESTAEKFGYSNKLFIIDNPFNNNFNIMRPSIMPNLLQVTADNIAHGISDLAIFEIG
PIYDGEARSKHVLSGIRTGNNLPRNHYNTDRKVDVFDAKADCIAALEFFNVNCDNLTIEKAEKEHYHPGKSGTLSFKNKV
AGYFGELHPNILDLFDIKQKVVGFEVILENIENLPVSRKKFIDYKYQSVKRDFAFIVNKDVEVGNIINVIKKSSELITEV
LIFDVYHGNNIEPNKMSIALSVTFCSPIHTLTEKEIQKESSAIVNLVCENTGGTLRTYR

Sequences:

>Translated_779_residues
MKFTLSWLLEYLETNASLEEITDKLTHIGLEVEDVIDNTKLAGFIVAEVLEVIQHPNADKLKLCKVDDGSKILQIVCGAN
NVREGMKTVLASLGSTLPESDFTIKPTKIRGVLSEGMLCSASELALVQEESEGIIELSDDYKVGDKFFNCDPVIDINVTP
NRGDCLGIYGIARDLAATGIGTLKTLSIPQLTSFINSPIDVEVTDGESFISGIYIANVKNQESPKWLKDRLESIGMRSIS
AIVDITNYIMISFSRPMHAYDAKKIEGELTVRKANDGEKFAGLNGKEYLLNKDISVIAGDKNIHGIAGIIGGKCSECTLE
TTDIFLESAWFDPISITKSSRQLGISTDASYRFARSVDPGFTFDGLNLAAKMILDLCGGETSSVVSAGSLDSVDTKVSFD
YQDVNKFGSVSVSPDEVLDILTKLGFSIDKKTEGNWNVQVPSWRPDVTIPADLIEEVTRIYGYDKIKEEPLPNNVEEVDS
TYDNLRILMTNRGFHEVLTWSFMSESTAEKFGYSNKLFIIDNPFNNNFNIMRPSIMPNLLQVTADNIAHGISDLAIFEIG
PIYDGEARSKHVLSGIRTGNNLPRNHYNTDRKVDVFDAKADCIAALEFFNVNCDNLTIEKAEKEHYHPGKSGTLSFKNKV
AGYFGELHPNILDLFDIKQKVVGFEVILENIENLPVSRKKFIDYKYQSVKRDFAFIVNKDVEVGNIINVIKKSSELITEV
LIFDVYHGNNIEPNKMSIALSVTFCSPIHTLTEKEIQKESSAIVNLVCENTGGTLRTYR
>Mature_779_residues
MKFTLSWLLEYLETNASLEEITDKLTHIGLEVEDVIDNTKLAGFIVAEVLEVIQHPNADKLKLCKVDDGSKILQIVCGAN
NVREGMKTVLASLGSTLPESDFTIKPTKIRGVLSEGMLCSASELALVQEESEGIIELSDDYKVGDKFFNCDPVIDINVTP
NRGDCLGIYGIARDLAATGIGTLKTLSIPQLTSFINSPIDVEVTDGESFISGIYIANVKNQESPKWLKDRLESIGMRSIS
AIVDITNYIMISFSRPMHAYDAKKIEGELTVRKANDGEKFAGLNGKEYLLNKDISVIAGDKNIHGIAGIIGGKCSECTLE
TTDIFLESAWFDPISITKSSRQLGISTDASYRFARSVDPGFTFDGLNLAAKMILDLCGGETSSVVSAGSLDSVDTKVSFD
YQDVNKFGSVSVSPDEVLDILTKLGFSIDKKTEGNWNVQVPSWRPDVTIPADLIEEVTRIYGYDKIKEEPLPNNVEEVDS
TYDNLRILMTNRGFHEVLTWSFMSESTAEKFGYSNKLFIIDNPFNNNFNIMRPSIMPNLLQVTADNIAHGISDLAIFEIG
PIYDGEARSKHVLSGIRTGNNLPRNHYNTDRKVDVFDAKADCIAALEFFNVNCDNLTIEKAEKEHYHPGKSGTLSFKNKV
AGYFGELHPNILDLFDIKQKVVGFEVILENIENLPVSRKKFIDYKYQSVKRDFAFIVNKDVEVGNIINVIKKSSELITEV
LIFDVYHGNNIEPNKMSIALSVTFCSPIHTLTEKEIQKESSAIVNLVCENTGGTLRTYR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=390, Percent_Identity=24.1025641025641, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1788006, Length=805, Percent_Identity=32.9192546583851, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17533403, Length=596, Percent_Identity=22.6510067114094, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 86358; Mature: 86358

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTLSWLLEYLETNASLEEITDKLTHIGLEVEDVIDNTKLAGFIVAEVLEVIQHPNADK
CEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
LKLCKVDDGSKILQIVCGANNVREGMKTVLASLGSTLPESDFTIKPTKIRGVLSEGMLCS
EEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHCCCEEC
ASELALVQEESEGIIELSDDYKVGDKFFNCDPVIDINVTPNRGDCLGIYGIARDLAATGI
CHHHEEEEECCCCEEEECCCCCCCCEEECCCCEEEEEECCCCCCEEEHHHHHHHHHHCCC
GTLKTLSIPQLTSFINSPIDVEVTDGESFISGIYIANVKNQESPKWLKDRLESIGMRSIS
CCEEECCCHHHHHHHCCCCEEEECCCCHHHCEEEEEECCCCCCCHHHHHHHHHCCHHHHH
AIVDITNYIMISFSRPMHAYDAKKIEGELTVRKANDGEKFAGLNGKEYLLNKDISVIAGD
HHHHHHCEEEEEECCCCCCCCCHHCCCEEEEEECCCCCCCCCCCCCEEEECCCCEEEECC
KNIHGIAGIIGGKCSECTLETTDIFLESAWFDPISITKSSRQLGISTDASYRFARSVDPG
CCCCEEHHHHCCCCCCCEEEHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEECCCCCC
FTFDGLNLAAKMILDLCGGETSSVVSAGSLDSVDTKVSFDYQDVNKFGSVSVSPDEVLDI
CEECHHHHHHHHHHHHHCCCHHHEEECCCCCCCCCEEEECHHHHHHCCCCCCCHHHHHHH
LTKLGFSIDKKTEGNWNVQVPSWRPDVTIPADLIEEVTRIYGYDKIKEEPLPNNVEEVDS
HHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCHHHHHC
TYDNLRILMTNRGFHEVLTWSFMSESTAEKFGYSNKLFIIDNPFNNNFNIMRPSIMPNLL
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCHHHHHHH
QVTADNIAHGISDLAIFEIGPIYDGEARSKHVLSGIRTGNNLPRNHYNTDRKVDVFDAKA
HHHHHHHHHCHHHEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCH
DCIAALEFFNVNCDNLTIEKAEKEHYHPGKSGTLSFKNKVAGYFGELHPNILDLFDIKQK
HHHHHHHHHCCCCCCEEEECCCHHCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHH
VVGFEVILENIENLPVSRKKFIDYKYQSVKRDFAFIVNKDVEVGNIINVIKKSSELITEV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHH
LIFDVYHGNNIEPNKMSIALSVTFCSPIHTLTEKEIQKESSAIVNLVCENTGGTLRTYR
HHEEEECCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHCEEEEEEEECCCCCEEECC
>Mature Secondary Structure
MKFTLSWLLEYLETNASLEEITDKLTHIGLEVEDVIDNTKLAGFIVAEVLEVIQHPNADK
CEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
LKLCKVDDGSKILQIVCGANNVREGMKTVLASLGSTLPESDFTIKPTKIRGVLSEGMLCS
EEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHCCCEEC
ASELALVQEESEGIIELSDDYKVGDKFFNCDPVIDINVTPNRGDCLGIYGIARDLAATGI
CHHHEEEEECCCCEEEECCCCCCCCEEECCCCEEEEEECCCCCCEEEHHHHHHHHHHCCC
GTLKTLSIPQLTSFINSPIDVEVTDGESFISGIYIANVKNQESPKWLKDRLESIGMRSIS
CCEEECCCHHHHHHHCCCCEEEECCCCHHHCEEEEEECCCCCCCHHHHHHHHHCCHHHHH
AIVDITNYIMISFSRPMHAYDAKKIEGELTVRKANDGEKFAGLNGKEYLLNKDISVIAGD
HHHHHHCEEEEEECCCCCCCCCHHCCCEEEEEECCCCCCCCCCCCCEEEECCCCEEEECC
KNIHGIAGIIGGKCSECTLETTDIFLESAWFDPISITKSSRQLGISTDASYRFARSVDPG
CCCCEEHHHHCCCCCCCEEEHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEECCCCCC
FTFDGLNLAAKMILDLCGGETSSVVSAGSLDSVDTKVSFDYQDVNKFGSVSVSPDEVLDI
CEECHHHHHHHHHHHHHCCCHHHEEECCCCCCCCCEEEECHHHHHHCCCCCCCHHHHHHH
LTKLGFSIDKKTEGNWNVQVPSWRPDVTIPADLIEEVTRIYGYDKIKEEPLPNNVEEVDS
HHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCHHHHHC
TYDNLRILMTNRGFHEVLTWSFMSESTAEKFGYSNKLFIIDNPFNNNFNIMRPSIMPNLL
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCHHHHHHH
QVTADNIAHGISDLAIFEIGPIYDGEARSKHVLSGIRTGNNLPRNHYNTDRKVDVFDAKA
HHHHHHHHHCHHHEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCH
DCIAALEFFNVNCDNLTIEKAEKEHYHPGKSGTLSFKNKVAGYFGELHPNILDLFDIKQK
HHHHHHHHHCCCCCCEEEECCCHHCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHH
VVGFEVILENIENLPVSRKKFIDYKYQSVKRDFAFIVNKDVEVGNIINVIKKSSELITEV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHH
LIFDVYHGNNIEPNKMSIALSVTFCSPIHTLTEKEIQKESSAIVNLVCENTGGTLRTYR
HHEEEECCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHCEEEEEEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA