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The map label for this gene is eno [H]

Identifier: 225630076

GI number: 225630076

Start: 262976

End: 264250

Strand: Direct

Name: eno [H]

Synonym: WRi_002510

Alternate gene names: 225630076

Gene position: 262976-264250 (Clockwise)

Preceding gene: 225630075

Following gene: 225630077

Centisome position: 18.19

GC content: 38.82

Gene sequence:

>1275_bases
ATGAATAAGATAATCAATAACGTATTTGCAAGAGAAATTTTAGATAGCAGGGGTTACCCCACTATTGAGGTAGAAATTGA
GCTCTGTGATGGCGCAATAGGCAGGGCATCTGTACCTTCTGGAGCTTCAACTGGTAAATTAGAAGCCTTGGAACTCAGGG
ACCAAGATGAGAAAAGGTATTGTGGTAAGGGAGTGCTGAAAGCTGTTCAAGCTGTAAATGGGATAATAGCAGATGAAATC
ATTGGAATGAATGCAGCGGACCAAAATGCAATTGATAAAGCATTAATTGAACTGGATGGAACAAAAAACAAATCAAAACT
TGGAGCAAATGCAACTTTGGGTGTGTCTCTTGCAGTTGCAAAAGCAGCAGCAAACAGTTTCAAAATGCCGCTATATAGAT
ATTTGGGAGGAAAGCAGACGAGTGTTATGCCGGTTCCACTCATTAACATAATTAATGGTGGAGTACATGCAGACAATAAG
CTCGATTTCCAAGAATTCATGATTCTTCCGGTCGGTGCTGAGACTTTCAGCGAAGCGATTAGAATATCTGCGGAGGTATT
CCACAACTTACGTAGCATTCTTAAGAAAAAAGGTTATAGCACAAATGTAGGGGATGAAGGTGGTTTTGCACCAAATATTG
AAAGTACTGAAGAAGCACTTGATTTGATCATATACGCTATAGAATCAGCAGGTTATTCAGCGCAAAGTGATTTTGCACTA
GGCCTTGATGTTGCTTCATCTACTTTTTATGAAGATGGAATTTACGAATTTGAAAGTAAGGGGCTTACTTCAGAAGAGTT
AACCGAATATTATTGTAACCTTGTGGAAAGATATCCAATAATTTCTATAGAAGATGCAATGAGTGAAGACGACTATGAAG
GCTGGAAATTGCTTACTGCAAAACTAGGGAATAAAATTCAATTGGTCGGGGATGATTTGTTTGTTACAAATTGTGAACTG
ATATGCAAAGGAATAGAGGAAAAAATGGCAAATGCTGTACTGATCAAGCCAAATCAAATAGGGACGTTAACAGAAACTTT
TGCTGCTATTGAAATGGCAAAATCAAATGGCTATAAAGCTGTTGTTTCTCATCGCTCAGGTGAAACAGAAGACACAACAA
TATCCCACATAGCAGTTGCGTCAAATTGCGGGCAAATAAAAACCGGGTCGCTATCGCGTTCTGATAGACTCGCGAAGTAT
AATGAGCTAATGAGAATAGAAAGCACGTTAGGAAAGGATGCTAAATATTATCGTGGGTTAGCATGGGTTTTATAG

Upstream 100 bases:

>100_bases
AGTCCAAGTGATATAGTATTATTTATTGGTGCTGGTAGTAATATAGCTAAGCTAGCAAAAGAAACTGCAGCACTTATTGC
GGAAGTTAAGGTTTAATGTA

Downstream 100 bases:

>100_bases
ACGAAGTAAAATTGTGTTTAAAAGCCGGTGACGGTGGTGATGGCTGTGCAAGTTTTCGTCGAGAAAAGTTCGTTGAATTT
GGTGGTCCAAATGGTGGTAA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MNKIINNVFAREILDSRGYPTIEVEIELCDGAIGRASVPSGASTGKLEALELRDQDEKRYCGKGVLKAVQAVNGIIADEI
IGMNAADQNAIDKALIELDGTKNKSKLGANATLGVSLAVAKAAANSFKMPLYRYLGGKQTSVMPVPLINIINGGVHADNK
LDFQEFMILPVGAETFSEAIRISAEVFHNLRSILKKKGYSTNVGDEGGFAPNIESTEEALDLIIYAIESAGYSAQSDFAL
GLDVASSTFYEDGIYEFESKGLTSEELTEYYCNLVERYPIISIEDAMSEDDYEGWKLLTAKLGNKIQLVGDDLFVTNCEL
ICKGIEEKMANAVLIKPNQIGTLTETFAAIEMAKSNGYKAVVSHRSGETEDTTISHIAVASNCGQIKTGSLSRSDRLAKY
NELMRIESTLGKDAKYYRGLAWVL

Sequences:

>Translated_424_residues
MNKIINNVFAREILDSRGYPTIEVEIELCDGAIGRASVPSGASTGKLEALELRDQDEKRYCGKGVLKAVQAVNGIIADEI
IGMNAADQNAIDKALIELDGTKNKSKLGANATLGVSLAVAKAAANSFKMPLYRYLGGKQTSVMPVPLINIINGGVHADNK
LDFQEFMILPVGAETFSEAIRISAEVFHNLRSILKKKGYSTNVGDEGGFAPNIESTEEALDLIIYAIESAGYSAQSDFAL
GLDVASSTFYEDGIYEFESKGLTSEELTEYYCNLVERYPIISIEDAMSEDDYEGWKLLTAKLGNKIQLVGDDLFVTNCEL
ICKGIEEKMANAVLIKPNQIGTLTETFAAIEMAKSNGYKAVVSHRSGETEDTTISHIAVASNCGQIKTGSLSRSDRLAKY
NELMRIESTLGKDAKYYRGLAWVL
>Mature_424_residues
MNKIINNVFAREILDSRGYPTIEVEIELCDGAIGRASVPSGASTGKLEALELRDQDEKRYCGKGVLKAVQAVNGIIADEI
IGMNAADQNAIDKALIELDGTKNKSKLGANATLGVSLAVAKAAANSFKMPLYRYLGGKQTSVMPVPLINIINGGVHADNK
LDFQEFMILPVGAETFSEAIRISAEVFHNLRSILKKKGYSTNVGDEGGFAPNIESTEEALDLIIYAIESAGYSAQSDFAL
GLDVASSTFYEDGIYEFESKGLTSEELTEYYCNLVERYPIISIEDAMSEDDYEGWKLLTAKLGNKIQLVGDDLFVTNCEL
ICKGIEEKMANAVLIKPNQIGTLTETFAAIEMAKSNGYKAVVSHRSGETEDTTISHIAVASNCGQIKTGSLSRSDRLAKY
NELMRIESTLGKDAKYYRGLAWVL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=426, Percent_Identity=52.5821596244132, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=426, Percent_Identity=52.112676056338, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=426, Percent_Identity=52.112676056338, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=426, Percent_Identity=51.6431924882629, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI301897479, Length=424, Percent_Identity=47.6415094339623, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=24.7761194029851, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=24.7761194029851, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=24.7761194029851, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1789141, Length=423, Percent_Identity=58.1560283687943, Blast_Score=474, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71995829, Length=428, Percent_Identity=50.9345794392523, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17536383, Length=428, Percent_Identity=50.9345794392523, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=210, Percent_Identity=45.2380952380952, Blast_Score=181, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=429, Percent_Identity=51.0489510489511, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=430, Percent_Identity=49.0697674418605, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324974, Length=430, Percent_Identity=49.0697674418605, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=430, Percent_Identity=49.0697674418605, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=429, Percent_Identity=50.8158508158508, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=427, Percent_Identity=50.8196721311475, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=427, Percent_Identity=50.8196721311475, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=427, Percent_Identity=50.8196721311475, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=427, Percent_Identity=50.8196721311475, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=427, Percent_Identity=50.8196721311475, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17137654, Length=427, Percent_Identity=50.8196721311475, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46072; Mature: 46072

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIINNVFAREILDSRGYPTIEVEIELCDGAIGRASVPSGASTGKLEALELRDQDEKRY
CCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHHH
CGKGVLKAVQAVNGIIADEIIGMNAADQNAIDKALIELDGTKNKSKLGANATLGVSLAVA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHCCCCCEEHHHHHHH
KAAANSFKMPLYRYLGGKQTSVMPVPLINIINGGVHADNKLDFQEFMILPVGAETFSEAI
HHHHCCCCCHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHH
RISAEVFHNLRSILKKKGYSTNVGDEGGFAPNIESTEEALDLIIYAIESAGYSAQSDFAL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEE
GLDVASSTFYEDGIYEFESKGLTSEELTEYYCNLVERYPIISIEDAMSEDDYEGWKLLTA
EEEHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCCCEEEHH
KLGNKIQLVGDDLFVTNCELICKGIEEKMANAVLIKPNQIGTLTETFAAIEMAKSNGYKA
HHCCEEEEEECCEEEHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCEE
VVSHRSGETEDTTISHIAVASNCGQIKTGSLSRSDRLAKYNELMRIESTLGKDAKYYRGL
EEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
AWVL
HHCC
>Mature Secondary Structure
MNKIINNVFAREILDSRGYPTIEVEIELCDGAIGRASVPSGASTGKLEALELRDQDEKRY
CCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHHH
CGKGVLKAVQAVNGIIADEIIGMNAADQNAIDKALIELDGTKNKSKLGANATLGVSLAVA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHCCCCCEEHHHHHHH
KAAANSFKMPLYRYLGGKQTSVMPVPLINIINGGVHADNKLDFQEFMILPVGAETFSEAI
HHHHCCCCCHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHH
RISAEVFHNLRSILKKKGYSTNVGDEGGFAPNIESTEEALDLIIYAIESAGYSAQSDFAL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEE
GLDVASSTFYEDGIYEFESKGLTSEELTEYYCNLVERYPIISIEDAMSEDDYEGWKLLTA
EEEHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCCCEEEHH
KLGNKIQLVGDDLFVTNCELICKGIEEKMANAVLIKPNQIGTLTETFAAIEMAKSNGYKA
HHCCEEEEEECCEEEHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCEE
VVSHRSGETEDTTISHIAVASNCGQIKTGSLSRSDRLAKYNELMRIESTLGKDAKYYRGL
EEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
AWVL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA