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The map label for this gene is yleA [C]

Identifier: 225630069

GI number: 225630069

Start: 253270

End: 254496

Strand: Direct

Name: yleA [C]

Synonym: WRi_002440

Alternate gene names: 225630069

Gene position: 253270-254496 (Clockwise)

Preceding gene: 225630067

Following gene: 225630070

Centisome position: 17.52

GC content: 33.99

Gene sequence:

>1227_bases
ATGAATGAAGTGATAACATTTGGTTGTCGTCTAAATTTTTACGAGAGCGAGTTAATCAAAGAAGCATTAAAAAAAGCAAA
GAGAGAAAATGTTGTTGTGGTGCATAGCTGTGCAGTGACAAACGAAGCAGAACGTCAGGTAAAGCAAAAGATACGTAAGA
TTTACAAAAATGATCCAAATAAAGAAATTATCGTAGTTGGTTGTGCTGTTCAGTTGGATCCAGAAGTTTACAGTAGCATT
CCTGGTGTAAGTAAAGTGCTCGGTAATCAAGATAAGCTAAAAGCTGAAAATTACTTACTAAATGACGATAAAATCTTGGT
CAGTGATAACCAAGTAGAACCTGTTCTAATTAATGGATTTGAAGATAAGTCAAGGGCATTTATTGAAATTCAAAATGGTT
GTAATCATAGCTGCACATTTTGTTCAATTACTGAGGCAAGAGGGAATAATCGATCTGTGCCAATAAACAGTATTATAGAG
CAAATCAGGATCTTTGTAGAAAATGGATATCAAGAAGTAGTGTTTACAGGGGTTGATATTACTGACTTTGGTACAGATTT
GTTGGGTAAGCCATCACTTGGTTCAATGATTAGGAGAGTTTTAAAGGATATACCAGAGTTGAAGAGGCTAAGACTTTCCT
CTATCGATGTTGCTGAAGTTGACGACGAATTAATGGATTTAATAGCTAATGAGTCAAGACTTATGCCTCATTTACATTTG
AGTCTACAATCTGGTAATAATTTAATACTAAAGAGAATGAAGCGTCGTCACAACAGAGAACAAGTGATAGAATTTTGTCA
TAAAATGAAGAGCTTGAGGCCTAATATAGCATTTGGCGCTGATATTATTGCTGGATTTCCAACAGAAACTGATGAAATGT
TTCAGGATACAGTTGATTTACTGAAAAAAACAAATATAGTTTACCTACATGCTTTTCCATATTCAGAGCGGAAAAATACA
CCTGCTGCACGAATGCCACAAGTACCAGAGAATGTGCGTAAAGAACGAGTAAAAAATTTAAGAGAAGTAAATAAAGAAAT
TATGAGCAGTTTTTGTCAATCCTTGATCAACACTAAGCAAAGTGTTCTGGTTGAGCAAAATAATGTCGGTAGAGCAGAAA
ATTTTGCATTAATAAAGCTTGAATCAAAAGCTCAAGCCAAGAGTATTGTGAAAGTTAATGTTAAAGGAGTGGAAAATAAT
TATCTAATTGGAAATATTTTTTCTTAA

Upstream 100 bases:

>100_bases
TGCTGCTTGCATAGCATTTATTTACAAAAAAATAGTCATGCAAGAGACTTTGCTTGGATATTTGATATAATTTAGGTCTA
TTAAAGCAAAAAATACTAAC

Downstream 100 bases:

>100_bases
TTTTTGTACATTGAAGAAATATACGATATAGTAATTATACTGTAAATGATAATTGTGAATTCATTTATGATAACTAGAAT
ATCTATTACTTTATTTTTGA

Product: MiaB-like tRNA modifying enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MNEVITFGCRLNFYESELIKEALKKAKRENVVVVHSCAVTNEAERQVKQKIRKIYKNDPNKEIIVVGCAVQLDPEVYSSI
PGVSKVLGNQDKLKAENYLLNDDKILVSDNQVEPVLINGFEDKSRAFIEIQNGCNHSCTFCSITEARGNNRSVPINSIIE
QIRIFVENGYQEVVFTGVDITDFGTDLLGKPSLGSMIRRVLKDIPELKRLRLSSIDVAEVDDELMDLIANESRLMPHLHL
SLQSGNNLILKRMKRRHNREQVIEFCHKMKSLRPNIAFGADIIAGFPTETDEMFQDTVDLLKKTNIVYLHAFPYSERKNT
PAARMPQVPENVRKERVKNLREVNKEIMSSFCQSLINTKQSVLVEQNNVGRAENFALIKLESKAQAKSIVKVNVKGVENN
YLIGNIFS

Sequences:

>Translated_408_residues
MNEVITFGCRLNFYESELIKEALKKAKRENVVVVHSCAVTNEAERQVKQKIRKIYKNDPNKEIIVVGCAVQLDPEVYSSI
PGVSKVLGNQDKLKAENYLLNDDKILVSDNQVEPVLINGFEDKSRAFIEIQNGCNHSCTFCSITEARGNNRSVPINSIIE
QIRIFVENGYQEVVFTGVDITDFGTDLLGKPSLGSMIRRVLKDIPELKRLRLSSIDVAEVDDELMDLIANESRLMPHLHL
SLQSGNNLILKRMKRRHNREQVIEFCHKMKSLRPNIAFGADIIAGFPTETDEMFQDTVDLLKKTNIVYLHAFPYSERKNT
PAARMPQVPENVRKERVKNLREVNKEIMSSFCQSLINTKQSVLVEQNNVGRAENFALIKLESKAQAKSIVKVNVKGVENN
YLIGNIFS
>Mature_408_residues
MNEVITFGCRLNFYESELIKEALKKAKRENVVVVHSCAVTNEAERQVKQKIRKIYKNDPNKEIIVVGCAVQLDPEVYSSI
PGVSKVLGNQDKLKAENYLLNDDKILVSDNQVEPVLINGFEDKSRAFIEIQNGCNHSCTFCSITEARGNNRSVPINSIIE
QIRIFVENGYQEVVFTGVDITDFGTDLLGKPSLGSMIRRVLKDIPELKRLRLSSIDVAEVDDELMDLIANESRLMPHLHL
SLQSGNNLILKRMKRRHNREQVIEFCHKMKSLRPNIAFGADIIAGFPTETDEMFQDTVDLLKKTNIVYLHAFPYSERKNT
PAARMPQVPENVRKERVKNLREVNKEIMSSFCQSLINTKQSVLVEQNNVGRAENFALIKLESKAQAKSIVKVNVKGVENN
YLIGNIFS

Specific function: Unknown

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 MTTase N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=398, Percent_Identity=28.1407035175879, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI28872784, Length=416, Percent_Identity=25.2403846153846, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI28872782, Length=416, Percent_Identity=25.2403846153846, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1786882, Length=432, Percent_Identity=26.3888888888889, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787057, Length=437, Percent_Identity=24.0274599542334, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17553146, Length=437, Percent_Identity=26.3157894736842, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71996771, Length=219, Percent_Identity=30.1369863013699, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356207, Length=420, Percent_Identity=27.1428571428571, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI19922432, Length=365, Percent_Identity=28.4931506849315, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006467
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 46292; Mature: 46292

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVITFGCRLNFYESELIKEALKKAKRENVVVVHSCAVTNEAERQVKQKIRKIYKNDPN
CCCEEEEEEEECHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCC
KEIIVVGCAVQLDPEVYSSIPGVSKVLGNQDKLKAENYLLNDDKILVSDNQVEPVLINGF
CEEEEEEEEEEECHHHHHHCCCHHHHHCCCHHHCCCCEEECCCEEEEECCCCCEEEEECC
EDKSRAFIEIQNGCNHSCTFCSITEARGNNRSVPINSIIEQIRIFVENGYQEVVFTGVDI
CCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCH
TDFGTDLLGKPSLGSMIRRVLKDIPELKRLRLSSIDVAEVDDELMDLIANESRLMPHLHL
HHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEE
SLQSGNNLILKRMKRRHNREQVIEFCHKMKSLRPNIAFGADIIAGFPTETDEMFQDTVDL
EECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEECCHHHCCCCCCHHHHHHHHHHH
LKKTNIVYLHAFPYSERKNTPAARMPQVPENVRKERVKNLREVNKEIMSSFCQSLINTKQ
HHHCCEEEEEECCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SVLVEQNNVGRAENFALIKLESKAQAKSIVKVNVKGVENNYLIGNIFS
HEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MNEVITFGCRLNFYESELIKEALKKAKRENVVVVHSCAVTNEAERQVKQKIRKIYKNDPN
CCCEEEEEEEECHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCC
KEIIVVGCAVQLDPEVYSSIPGVSKVLGNQDKLKAENYLLNDDKILVSDNQVEPVLINGF
CEEEEEEEEEEECHHHHHHCCCHHHHHCCCHHHCCCCEEECCCEEEEECCCCCEEEEECC
EDKSRAFIEIQNGCNHSCTFCSITEARGNNRSVPINSIIEQIRIFVENGYQEVVFTGVDI
CCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCH
TDFGTDLLGKPSLGSMIRRVLKDIPELKRLRLSSIDVAEVDDELMDLIANESRLMPHLHL
HHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEE
SLQSGNNLILKRMKRRHNREQVIEFCHKMKSLRPNIAFGADIIAGFPTETDEMFQDTVDL
EECCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEECCHHHCCCCCCHHHHHHHHHHH
LKKTNIVYLHAFPYSERKNTPAARMPQVPENVRKERVKNLREVNKEIMSSFCQSLINTKQ
HHHCCEEEEEECCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SVLVEQNNVGRAENFALIKLESKAQAKSIVKVNVKGVENNYLIGNIFS
HEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]