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The map label for this gene is prfA [H]

Identifier: 225630064

GI number: 225630064

Start: 242527

End: 243600

Strand: Direct

Name: prfA [H]

Synonym: WRi_002340

Alternate gene names: 225630064

Gene position: 242527-243600 (Clockwise)

Preceding gene: 225630059

Following gene: 225630066

Centisome position: 16.77

GC content: 36.13

Gene sequence:

>1074_bases
ATGGAAAATAATTTACAAGACTTAAAGAAAAAATTTAATGATATAGAGAGAAATTTGGAAAATCCTACCAATTTAAGTCA
AAAAGAATTCATTACCCTTTCAAAGGAATACTCTGAGCTCAGGCCAATAATCAAGATAATTGACGAATATAACACGCTGA
AAGAGGAAATTTCAGACTTAGAAGAAATAATGAAAGATGAAAACAGCGAAGGTGATATAAAAGAGTTAGCAAAAGAAGAA
TTCTTCGAAAAGCACAAGGTATTATTACCAAAAATAAAAGCAAAATTAAAGTTAGCATTATTGCCCAAAGATGAAGATGA
CTCAAGAAATGCAATATTAGAAATTAGAGCAGGTACAGGCGGAGAAGAAGCAGCATTATTTGCAGCAATGTTATTTCGTA
TGTATCAAAAATATGCAGAAAGAAGAAATTGGAAGTTCGAGCCAATAAGCATTTCTAATACAGGTATAGGTGGCTATAAG
GAAGCTTCTGCACTCATTAATGGAACAGAAGTTTTTGCAAGGTTGAAATTTGAATCAGGGGTGCACAGAGTACAGAGAGT
GCCAGAAACTGAATCCTCAGGAAGGTTGCATACCTCTGCCGCTACTGTTGCGATATTACCTGAAGTAGAAGAAGTTGACT
TTAAAATAGAAGAAAAAGACTTACGAATAGATGTTTATAGATCCAGTGGTCCTGGAGGGCAATCAGTGAATACAACTGAC
AGCGCAGTAAGGGTCACCCACTTGCCAACAGGGATAGTTGTGATACAGCAAGATGAAAAATCGCAGCATAAAAATAAAGC
TAAAGCGCTCAAAGTATTGAGGGCAAGGCTATACGAAATTGAAAGACAAAAAAAAGAAATGGAAAGGTCAACAATGAGGA
AAAGTCAGATTGGCTCTGGTGATCGTTCCGAGCGCATAAGAACATATAATTTTCCACAATCAAGAATAACAGACCACAGA
ATTAATCTAACTTCACATCGGCTAGAGCAGATCATAAAAGAAGGTGAACTAGATGAATTTATTGAGGCATTAATTTCACG
CAATGAAGCAGAAAGATTGGCCGGGGAAAGTTAG

Upstream 100 bases:

>100_bases
TTCAACTAGAAGGTAGTAGGTATATTATAGTGTTATTGCTTGATATAAGAAATTTTTTATTTCAGTATATATTACTTAGC
TAGATCTGTGCTATATGGAT

Downstream 100 bases:

>100_bases
CAGCCTACAATGGAATATCTTTATTAATTACATAGGATCTTTTAAACGCAATAGATAGACAGTAAAAACAAGTTCTAGTA
CTATCGCGGATAAAACAAGA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MENNLQDLKKKFNDIERNLENPTNLSQKEFITLSKEYSELRPIIKIIDEYNTLKEEISDLEEIMKDENSEGDIKELAKEE
FFEKHKVLLPKIKAKLKLALLPKDEDDSRNAILEIRAGTGGEEAALFAAMLFRMYQKYAERRNWKFEPISISNTGIGGYK
EASALINGTEVFARLKFESGVHRVQRVPETESSGRLHTSAATVAILPEVEEVDFKIEEKDLRIDVYRSSGPGGQSVNTTD
SAVRVTHLPTGIVVIQQDEKSQHKNKAKALKVLRARLYEIERQKKEMERSTMRKSQIGSGDRSERIRTYNFPQSRITDHR
INLTSHRLEQIIKEGELDEFIEALISRNEAERLAGES

Sequences:

>Translated_357_residues
MENNLQDLKKKFNDIERNLENPTNLSQKEFITLSKEYSELRPIIKIIDEYNTLKEEISDLEEIMKDENSEGDIKELAKEE
FFEKHKVLLPKIKAKLKLALLPKDEDDSRNAILEIRAGTGGEEAALFAAMLFRMYQKYAERRNWKFEPISISNTGIGGYK
EASALINGTEVFARLKFESGVHRVQRVPETESSGRLHTSAATVAILPEVEEVDFKIEEKDLRIDVYRSSGPGGQSVNTTD
SAVRVTHLPTGIVVIQQDEKSQHKNKAKALKVLRARLYEIERQKKEMERSTMRKSQIGSGDRSERIRTYNFPQSRITDHR
INLTSHRLEQIIKEGELDEFIEALISRNEAERLAGES
>Mature_357_residues
MENNLQDLKKKFNDIERNLENPTNLSQKEFITLSKEYSELRPIIKIIDEYNTLKEEISDLEEIMKDENSEGDIKELAKEE
FFEKHKVLLPKIKAKLKLALLPKDEDDSRNAILEIRAGTGGEEAALFAAMLFRMYQKYAERRNWKFEPISISNTGIGGYK
EASALINGTEVFARLKFESGVHRVQRVPETESSGRLHTSAATVAILPEVEEVDFKIEEKDLRIDVYRSSGPGGQSVNTTD
SAVRVTHLPTGIVVIQQDEKSQHKNKAKALKVLRARLYEIERQKKEMERSTMRKSQIGSGDRSERIRTYNFPQSRITDHR
INLTSHRLEQIIKEGELDEFIEALISRNEAERLAGES

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=308, Percent_Identity=48.7012987012987, Blast_Score=282, Evalue=3e-76,
Organism=Homo sapiens, GI34577120, Length=366, Percent_Identity=39.0710382513661, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI166795305, Length=212, Percent_Identity=47.6415094339623, Blast_Score=196, Evalue=2e-50,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=50.4225352112676, Blast_Score=340, Evalue=9e-95,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=39.8230088495575, Blast_Score=220, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=322, Percent_Identity=39.4409937888199, Blast_Score=198, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=330, Percent_Identity=43.030303030303, Blast_Score=234, Evalue=1e-62,
Organism=Drosophila melanogaster, GI19921226, Length=325, Percent_Identity=42.1538461538462, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40963; Mature: 40963

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNLQDLKKKFNDIERNLENPTNLSQKEFITLSKEYSELRPIIKIIDEYNTLKEEISDL
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEIMKDENSEGDIKELAKEEFFEKHKVLLPKIKAKLKLALLPKDEDDSRNAILEIRAGTG
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEEECCCC
GEEAALFAAMLFRMYQKYAERRNWKFEPISISNTGIGGYKEASALINGTEVFARLKFESG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VHRVQRVPETESSGRLHTSAATVAILPEVEEVDFKIEEKDLRIDVYRSSGPGGQSVNTTD
HHHHHHCCCCCCCCCEEECCEEEEECCCHHHCCCEEECCCEEEEEEECCCCCCCCCCCCC
SAVRVTHLPTGIVVIQQDEKSQHKNKAKALKVLRARLYEIERQKKEMERSTMRKSQIGSG
CEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQSRITDHRINLTSHRLEQIIKEGELDEFIEALISRNEAERLAGES
CCCCCCEECCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MENNLQDLKKKFNDIERNLENPTNLSQKEFITLSKEYSELRPIIKIIDEYNTLKEEISDL
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEIMKDENSEGDIKELAKEEFFEKHKVLLPKIKAKLKLALLPKDEDDSRNAILEIRAGTG
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEEECCCC
GEEAALFAAMLFRMYQKYAERRNWKFEPISISNTGIGGYKEASALINGTEVFARLKFESG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VHRVQRVPETESSGRLHTSAATVAILPEVEEVDFKIEEKDLRIDVYRSSGPGGQSVNTTD
HHHHHHCCCCCCCCCEEECCEEEEECCCHHHCCCEEECCCEEEEEEECCCCCCCCCCCCC
SAVRVTHLPTGIVVIQQDEKSQHKNKAKALKVLRARLYEIERQKKEMERSTMRKSQIGSG
CEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQSRITDHRINLTSHRLEQIIKEGELDEFIEALISRNEAERLAGES
CCCCCCEECCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA