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The map label for this gene is fucA [C]

Identifier: 225630032

GI number: 225630032

Start: 201361

End: 202068

Strand: Reverse

Name: fucA [C]

Synonym: WRi_002000

Alternate gene names: 225630032

Gene position: 202068-201361 (Counterclockwise)

Preceding gene: 225630034

Following gene: 225630026

Centisome position: 13.98

GC content: 34.75

Gene sequence:

>708_bases
TTGTTAACAAAGAGCAGAGTAAAAAGGGATCTAGTCTATGCCTATCAGATTTTGTCCTACCTTAAGTTCGATGATCATAC
ATATACTCACCTTTCAGTGCGTTCTGAAGATCAAAAGTCGTTTTATATTTATCCGTTTGGTATGCGTTTTGATGAAGTAG
ATGAACGTTCGTTAATGAAAGTATCATTTGATGGAGATGTAGTTGAAGGCAAAGAATATCAATATAATAGAACTGGCTAT
ATAATTCATGGTTTTGTTTATCAAGCAAGGAAAGATATTCAAGCAATTTTTCACTTGCATACACCTTCTATTGTAGCGGT
TTCTTCTCTCAAAGATGGGCTGCTTCCTATAAGTCAATGGGCACTGCACTTTTACAATAAAATCTCTTACCATAATTATA
ATTCCCTAGCACTTAGCGATACAGAAGGAAAGAGGTTAATAGCTGATCTGAAAGAAAATTTTGTCATGTTAATGCGTAAT
CATGGATCTATAATGTGTGGTCGGAGTATACAAGAAGCAATGTTTTACACGTATCATTTAGAACAAGCGTGCAAAACTCA
ATGTTTAACGTTAGCAATGAATAAGGAGTTATCAATTCCAAGTGAAGAAATTTGCTCAAAAGCTGTAAAGGATCTCCTAT
CTTTTGAAAGCAATCTTGGTGAGAGGGATTGGCACGCATGGGTTAGACTAATTAAAAGTAAGTTGTAA

Upstream 100 bases:

>100_bases
TTGGTATTCAGAAAGCAAGACAGAGTGGCATAGACTTATTAGTTTACTACAAGTCTCTTTTCGCGTATAAACCTTCAGAA
GATTCTAACAGAGGATTGTG

Downstream 100 bases:

>100_bases
GTGACCTTACCCAGAAAAAGTAGAGAGAAAGTTAAGACGTTTTTGGAGTAAAATAAAACGTTTTTTCAAAATATGACAAA
TAGAAGAGAATATACAGCAG

Product: hypothetical protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MLTKSRVKRDLVYAYQILSYLKFDDHTYTHLSVRSEDQKSFYIYPFGMRFDEVDERSLMKVSFDGDVVEGKEYQYNRTGY
IIHGFVYQARKDIQAIFHLHTPSIVAVSSLKDGLLPISQWALHFYNKISYHNYNSLALSDTEGKRLIADLKENFVMLMRN
HGSIMCGRSIQEAMFYTYHLEQACKTQCLTLAMNKELSIPSEEICSKAVKDLLSFESNLGERDWHAWVRLIKSKL

Sequences:

>Translated_235_residues
MLTKSRVKRDLVYAYQILSYLKFDDHTYTHLSVRSEDQKSFYIYPFGMRFDEVDERSLMKVSFDGDVVEGKEYQYNRTGY
IIHGFVYQARKDIQAIFHLHTPSIVAVSSLKDGLLPISQWALHFYNKISYHNYNSLALSDTEGKRLIADLKENFVMLMRN
HGSIMCGRSIQEAMFYTYHLEQACKTQCLTLAMNKELSIPSEEICSKAVKDLLSFESNLGERDWHAWVRLIKSKL
>Mature_235_residues
MLTKSRVKRDLVYAYQILSYLKFDDHTYTHLSVRSEDQKSFYIYPFGMRFDEVDERSLMKVSFDGDVVEGKEYQYNRTGY
IIHGFVYQARKDIQAIFHLHTPSIVAVSSLKDGLLPISQWALHFYNKISYHNYNSLALSDTEGKRLIADLKENFVMLMRN
HGSIMCGRSIQEAMFYTYHLEQACKTQCLTLAMNKELSIPSEEICSKAVKDLLSFESNLGERDWHAWVRLIKSKL

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI29826321, Length=186, Percent_Identity=36.5591397849462, Blast_Score=127, Evalue=7e-30,
Organism=Homo sapiens, GI29826319, Length=186, Percent_Identity=36.5591397849462, Blast_Score=127, Evalue=7e-30,
Organism=Homo sapiens, GI29826325, Length=187, Percent_Identity=36.3636363636364, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI29826323, Length=187, Percent_Identity=36.3636363636364, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI89276787, Length=182, Percent_Identity=33.5164835164835, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI297307160, Length=182, Percent_Identity=33.5164835164835, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI9257190, Length=182, Percent_Identity=33.5164835164835, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI297307158, Length=182, Percent_Identity=33.5164835164835, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI9257192, Length=182, Percent_Identity=33.5164835164835, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI9951927, Length=186, Percent_Identity=31.7204301075269, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI62912453, Length=186, Percent_Identity=31.7204301075269, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI9943848, Length=186, Percent_Identity=31.7204301075269, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115533218, Length=246, Percent_Identity=31.7073170731707, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI115533216, Length=246, Percent_Identity=31.7073170731707, Blast_Score=143, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI71989560, Length=240, Percent_Identity=25.4166666666667, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71989550, Length=240, Percent_Identity=25.4166666666667, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71989555, Length=240, Percent_Identity=25.4166666666667, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71989541, Length=240, Percent_Identity=25.4166666666667, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71989544, Length=240, Percent_Identity=25.4166666666667, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281363763, Length=171, Percent_Identity=35.0877192982456, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281363757, Length=171, Percent_Identity=35.0877192982456, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17136738, Length=171, Percent_Identity=35.0877192982456, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281363755, Length=171, Percent_Identity=35.0877192982456, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281363751, Length=171, Percent_Identity=35.0877192982456, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24655690, Length=171, Percent_Identity=35.0877192982456, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24655685, Length=171, Percent_Identity=35.0877192982456, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281363761, Length=171, Percent_Identity=35.0877192982456, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281363759, Length=171, Percent_Identity=35.0877192982456, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281363753, Length=171, Percent_Identity=35.0877192982456, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 27468; Mature: 27468

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKSRVKRDLVYAYQILSYLKFDDHTYTHLSVRSEDQKSFYIYPFGMRFDEVDERSLMK
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEE
VSFDGDVVEGKEYQYNRTGYIIHGFVYQARKDIQAIFHLHTPSIVAVSSLKDGLLPISQW
EECCCCEECCCCEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCHHHHH
ALHFYNKISYHNYNSLALSDTEGKRLIADLKENFVMLMRNHGSIMCGRSIQEAMFYTYHL
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHEEEEECCCCEEECCHHHHHHHHHHHH
EQACKTQCLTLAMNKELSIPSEEICSKAVKDLLSFESNLGERDWHAWVRLIKSKL
HHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTKSRVKRDLVYAYQILSYLKFDDHTYTHLSVRSEDQKSFYIYPFGMRFDEVDERSLMK
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEE
VSFDGDVVEGKEYQYNRTGYIIHGFVYQARKDIQAIFHLHTPSIVAVSSLKDGLLPISQW
EECCCCEECCCCEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCHHHHH
ALHFYNKISYHNYNSLALSDTEGKRLIADLKENFVMLMRNHGSIMCGRSIQEAMFYTYHL
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHEEEEECCCCEEECCHHHHHHHHHHHH
EQACKTQCLTLAMNKELSIPSEEICSKAVKDLLSFESNLGERDWHAWVRLIKSKL
HHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]