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The map label for this gene is guaA

Identifier: 225630020

GI number: 225630020

Start: 182725

End: 184287

Strand: Reverse

Name: guaA

Synonym: WRi_001820

Alternate gene names: 225630020

Gene position: 184287-182725 (Counterclockwise)

Preceding gene: 225630021

Following gene: 225630018

Centisome position: 12.75

GC content: 36.4

Gene sequence:

>1563_bases
TTGTCAGCAATTGCCATTATTGATTTTGGTTCACAGTTTACACAGCTTATCGCAAGACAAGTAAGAGGAATGGGTGTTTA
TTGTGAGATATTTCCAAGCAACATCAGTTTTGAAACAATATCAAAATTCAATGGGTTTATTCTCTCTGGAGGACCACAAT
CTGTAAATGATGATTGTTCTGAAACAAGTAGAGTAGTACATGAAATTATAAAACTTAATGAGGCAACAAGCGTTCCTATA
CTTGGAATATGCTATGGACAGCAACTCATTTGTCATTATTTTGGAGCAAAAGTAAAAGAGAGTTTCAAACAGGAGTTTGG
TAGAACTAAAATCAAGATACTAAAAGAATCCCCCATTGTAAAGGATACCTGGGATGTTAATTCTGAAGTGGATGTATTAA
TGAACCATGCGGACAGTGTTGATACTATACCACAGGGATTTACTGTTATCGCATCAGGTGTGATAAATCAAACAATTGCA
ATGATTGTCAACGAACAGCGGAAGATTTACTGTACTCAGTTCCATCCTGAGGTTAAGCCCACAACAAATGGCAGTAAATT
GCTCTCTAACTTCTTGGATATTGCAAATTGCAAAAGAGACTGGACAATGAAGTCGTTTATTGAGGAGCAAAAGGAAAAAA
TCAAAAATGTAGTAGGAGAGAAAAAAGTAATCGCTGCAGTAAGTGGTGGGGTTGATTCAAGCGTTGCAGCGGCTCTCACA
CATAAAGCTATAGGAAAACAATTGAACTGTATTTTTATCGATACTGGGTTGTTACGCAAGAACCAAACCATCGCTATGTT
GAAAGAGATTCCGATAAATTACGTTGATAAATCAAATTTATTTTTGAGTAGGTTGAAGGGAATAACTGATCCAGAAGAAA
AGCGAAAAATTATCGGTAACACTTTTATTGAAGTGTTTGAAGAAGAAGCAAAAAAAATAGGTGATGTGGATTTTTTGATG
CAAGGTACCATCTACTCTGATGTAGTTGAGTCAGGGCATGCCTCAGACAACACTAGTACAATTAAGTCTCACCATAATGT
TGGTGGGTTGCCAGAAAAGATGAATCTGAAGTTAGTGGAGCCTTTACGTTACCTCTTTAAAGATGAGGTAAGGCTACTTG
GAAAAGAAATTGGGCTTTCAGACGAGATAATATTTCAACATCCATTTCCTGGACCCGGACTTGCAGTGAGGATTATAAGT
GAAGTCGATAAAGAAAAGGTGCAAATATTGCAAGAAGTAGATGAAATATATATTAATACGATGAAAAATTACGATCTATA
TGATAAGATATGGCAAGCTTTTGCTGTATTATTACCAATAAAAACTGTAGGCGTTATGGGAGATGGTCGTACGTATGGAT
ATGTTTGTGCTTTAAGGGCTGTAACATCATCTGATGGTATGACAGCCGATGCATTTCCATTTGAAGATAAGGATCAACAC
TTGCTAGTATTTTGGGATTTTTTACGTAATGTCGGCAGTATAATTGTTAATAATGTTCCCGGAGTAAATAGAGTTGTTTA
TGATATCACTTCTAAACCACCCGCAACTATTGAGTGGGAGTAA

Upstream 100 bases:

>100_bases
ACTTGTACCACGCGTTAAAGCTTTTAAACAGGTCTTGGTTTACTTACTCCATTATTTTCTTTATTATCAGATATCATTTT
AAATAATATGGAGGAGCAAA

Downstream 100 bases:

>100_bases
ATCAAAAAGATCCCGCTGCTTGTTAGCTGGATCTAGAGATACCGCGAATGAATCGCGGTATGACGGTTCGCGGCAACATG
ACGATAACCTCGTCATCCCG

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSAIAIIDFGSQFTQLIARQVRGMGVYCEIFPSNISFETISKFNGFILSGGPQSVNDDCSETSRVVHEIIKLNEATSVPI
LGICYGQQLICHYFGAKVKESFKQEFGRTKIKILKESPIVKDTWDVNSEVDVLMNHADSVDTIPQGFTVIASGVINQTIA
MIVNEQRKIYCTQFHPEVKPTTNGSKLLSNFLDIANCKRDWTMKSFIEEQKEKIKNVVGEKKVIAAVSGGVDSSVAAALT
HKAIGKQLNCIFIDTGLLRKNQTIAMLKEIPINYVDKSNLFLSRLKGITDPEEKRKIIGNTFIEVFEEEAKKIGDVDFLM
QGTIYSDVVESGHASDNTSTIKSHHNVGGLPEKMNLKLVEPLRYLFKDEVRLLGKEIGLSDEIIFQHPFPGPGLAVRIIS
EVDKEKVQILQEVDEIYINTMKNYDLYDKIWQAFAVLLPIKTVGVMGDGRTYGYVCALRAVTSSDGMTADAFPFEDKDQH
LLVFWDFLRNVGSIIVNNVPGVNRVVYDITSKPPATIEWE

Sequences:

>Translated_520_residues
MSAIAIIDFGSQFTQLIARQVRGMGVYCEIFPSNISFETISKFNGFILSGGPQSVNDDCSETSRVVHEIIKLNEATSVPI
LGICYGQQLICHYFGAKVKESFKQEFGRTKIKILKESPIVKDTWDVNSEVDVLMNHADSVDTIPQGFTVIASGVINQTIA
MIVNEQRKIYCTQFHPEVKPTTNGSKLLSNFLDIANCKRDWTMKSFIEEQKEKIKNVVGEKKVIAAVSGGVDSSVAAALT
HKAIGKQLNCIFIDTGLLRKNQTIAMLKEIPINYVDKSNLFLSRLKGITDPEEKRKIIGNTFIEVFEEEAKKIGDVDFLM
QGTIYSDVVESGHASDNTSTIKSHHNVGGLPEKMNLKLVEPLRYLFKDEVRLLGKEIGLSDEIIFQHPFPGPGLAVRIIS
EVDKEKVQILQEVDEIYINTMKNYDLYDKIWQAFAVLLPIKTVGVMGDGRTYGYVCALRAVTSSDGMTADAFPFEDKDQH
LLVFWDFLRNVGSIIVNNVPGVNRVVYDITSKPPATIEWE
>Mature_519_residues
SAIAIIDFGSQFTQLIARQVRGMGVYCEIFPSNISFETISKFNGFILSGGPQSVNDDCSETSRVVHEIIKLNEATSVPIL
GICYGQQLICHYFGAKVKESFKQEFGRTKIKILKESPIVKDTWDVNSEVDVLMNHADSVDTIPQGFTVIASGVINQTIAM
IVNEQRKIYCTQFHPEVKPTTNGSKLLSNFLDIANCKRDWTMKSFIEEQKEKIKNVVGEKKVIAAVSGGVDSSVAAALTH
KAIGKQLNCIFIDTGLLRKNQTIAMLKEIPINYVDKSNLFLSRLKGITDPEEKRKIIGNTFIEVFEEEAKKIGDVDFLMQ
GTIYSDVVESGHASDNTSTIKSHHNVGGLPEKMNLKLVEPLRYLFKDEVRLLGKEIGLSDEIIFQHPFPGPGLAVRIISE
VDKEKVQILQEVDEIYINTMKNYDLYDKIWQAFAVLLPIKTVGVMGDGRTYGYVCALRAVTSSDGMTADAFPFEDKDQHL
LVFWDFLRNVGSIIVNNVPGVNRVVYDITSKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=571, Percent_Identity=35.0262697022767, Blast_Score=266, Evalue=2e-71,
Organism=Escherichia coli, GI1788854, Length=533, Percent_Identity=46.9043151969981, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI71992717, Length=584, Percent_Identity=35.445205479452, Blast_Score=281, Evalue=7e-76,
Organism=Caenorhabditis elegans, GI71992710, Length=584, Percent_Identity=35.445205479452, Blast_Score=281, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI133901714, Length=584, Percent_Identity=35.445205479452, Blast_Score=281, Evalue=8e-76,
Organism=Saccharomyces cerevisiae, GI6323873, Length=534, Percent_Identity=47.565543071161, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI281365319, Length=563, Percent_Identity=34.1030195381883, Blast_Score=251, Evalue=6e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_WOLPM (Q73IH1)

Other databases:

- EMBL:   AE017196
- RefSeq:   NP_966007.1
- ProteinModelPortal:   Q73IH1
- SMR:   Q73IH1
- EnsemblBacteria:   EBWOLT00000004077
- GeneID:   2737681
- GenomeReviews:   AE017196_GR
- KEGG:   wol:WD0195
- TIGR:   WD_0195
- GeneTree:   EBGT00050000031318
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- ProtClustDB:   PRK00074
- BioCyc:   WPIP955:WD_0195-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 58078; Mature: 57946

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 84-84 ACT_SITE 175-175 ACT_SITE 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIAIIDFGSQFTQLIARQVRGMGVYCEIFPSNISFETISKFNGFILSGGPQSVNDDCS
CCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCEEEECCCCCCCCHHH
ETSRVVHEIIKLNEATSVPILGICYGQQLICHYFGAKVKESFKQEFGRTKIKILKESPIV
HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
KDTWDVNSEVDVLMNHADSVDTIPQGFTVIASGVINQTIAMIVNEQRKIYCTQFHPEVKP
CCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
TTNGSKLLSNFLDIANCKRDWTMKSFIEEQKEKIKNVVGEKKVIAAVSGGVDSSVAAALT
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
HKAIGKQLNCIFIDTGLLRKNQTIAMLKEIPINYVDKSNLFLSRLKGITDPEEKRKIIGN
HHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHH
TFIEVFEEEAKKIGDVDFLMQGTIYSDVVESGHASDNTSTIKSHHNVGGLPEKMNLKLVE
HHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCHHHH
PLRYLFKDEVRLLGKEIGLSDEIIFQHPFPGPGLAVRIISEVDKEKVQILQEVDEIYINT
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKNYDLYDKIWQAFAVLLPIKTVGVMGDGRTYGYVCALRAVTSSDGMTADAFPFEDKDQH
HCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHEEEEHHCCCCCCEECCCCCCCCCCE
LLVFWDFLRNVGSIIVNNVPGVNRVVYDITSKPPATIEWE
EEEHHHHHHHHHHHEECCCCCCCEEEEEECCCCCCCEECC
>Mature Secondary Structure 
SAIAIIDFGSQFTQLIARQVRGMGVYCEIFPSNISFETISKFNGFILSGGPQSVNDDCS
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCEEEECCCCCCCCHHH
ETSRVVHEIIKLNEATSVPILGICYGQQLICHYFGAKVKESFKQEFGRTKIKILKESPIV
HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
KDTWDVNSEVDVLMNHADSVDTIPQGFTVIASGVINQTIAMIVNEQRKIYCTQFHPEVKP
CCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
TTNGSKLLSNFLDIANCKRDWTMKSFIEEQKEKIKNVVGEKKVIAAVSGGVDSSVAAALT
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
HKAIGKQLNCIFIDTGLLRKNQTIAMLKEIPINYVDKSNLFLSRLKGITDPEEKRKIIGN
HHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHH
TFIEVFEEEAKKIGDVDFLMQGTIYSDVVESGHASDNTSTIKSHHNVGGLPEKMNLKLVE
HHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCHHHH
PLRYLFKDEVRLLGKEIGLSDEIIFQHPFPGPGLAVRIISEVDKEKVQILQEVDEIYINT
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKNYDLYDKIWQAFAVLLPIKTVGVMGDGRTYGYVCALRAVTSSDGMTADAFPFEDKDQH
HCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHEEEEHHCCCCCCEECCCCCCCCCCE
LLVFWDFLRNVGSIIVNNVPGVNRVVYDITSKPPATIEWE
EEEHHHHHHHHHHHEECCCCCCCEEEEEECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA