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The map label for this gene is ddl

Identifier: 225630001

GI number: 225630001

Start: 156517

End: 157470

Strand: Reverse

Name: ddl

Synonym: WRi_001550

Alternate gene names: 225630001

Gene position: 157470-156517 (Counterclockwise)

Preceding gene: 225630002

Following gene: 225630000

Centisome position: 10.89

GC content: 33.23

Gene sequence:

>954_bases
ATGCTTATGATTCCAACTATAGCAATTTTAAGTGGTGGATTTTCTTGTGAGAGAGAAATATCGCTTATGAGCGGAAAAGC
AGTAAAGAAGGCGCTCGATAGCCTTTCATATAATGCAATAGAAATAGATGTTGACAGCAATATTGCTGAAAAGCTTAAAA
AAATTAATCCGGGTCTTGCTTTTATCGCTCTGCATGGACCTTATGGTGAAGATGGCTGCATTCAAGGTTTATTGGAAATC
TTAGGTATAAAATATACACACTCAGGAGTTATGGCTTCTGCCGTTGCTATAAATAAAGTGATGTCAAAGCATATATTTCG
ATCCCTTAATATCGACACTCCAAAAGGTTATGTAATTAGCCGAGAAGATGTACTAAAAAATAATATTAAAATCGATTATC
CGTACGTCTTAAAACCAATTAATGAAGGTTCAAGTATTGGAGTGTACATAATTTTCTCGCACGAGGATTACTTAGAACTG
AAAGATAATAGTTCTACTATAATGGAAAAGATGATCGTAGAAGAATACATACCGGGCATAGAGTTACATACTGCTGTATT
GCTGGATGAAGCAATTGGCACTATAGAAGTACGACCAAAAAATAAATTCTATGATTATGAAGCAAAGTATACAGATGGAT
TTGCAGAACATATATTTCCTGCTAAAATTCCTGACAATATATATAAAATGACCTTGGAACACGCACTGAAAATACATCAA
TTTTTAGGATGCAAAACTATTTCCCGCTCAGATTTCCGTTATAATCCCAAAAATAACACTTTAAAAATGCTTGAGATTAA
TACACATCCTGGGTTTACTGAGTTATCGTTAGTGCCAGAAATTGCAAAATTGGCAAAAGGAATCAATTTTAATGAATTAG
TTAAAATTATTATTGAAGATAGTTTGCAGCACAAAAATATTAGGGATTTAAGTCATGTTGAGCAGTATTACTAG

Upstream 100 bases:

>100_bases
GTGGATAAAAAAAGTGCTTGAAGAAAGTTTATAGTTAGCCAAAGTAGGTATTTAATAAGCTTAATGTAACCATTATGTAT
GCTATATAGCAAAACATAGA

Downstream 100 bases:

>100_bases
AAGCCAAAGGAGTTTTTTGCGTAAGTGTGCCTTGGTTATTATCACAGCACTTTTTCTTACGCTAATACTTTACAGCTCAC
TTGATAAAATAATAAATCGA

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MLMIPTIAILSGGFSCEREISLMSGKAVKKALDSLSYNAIEIDVDSNIAEKLKKINPGLAFIALHGPYGEDGCIQGLLEI
LGIKYTHSGVMASAVAINKVMSKHIFRSLNIDTPKGYVISREDVLKNNIKIDYPYVLKPINEGSSIGVYIIFSHEDYLEL
KDNSSTIMEKMIVEEYIPGIELHTAVLLDEAIGTIEVRPKNKFYDYEAKYTDGFAEHIFPAKIPDNIYKMTLEHALKIHQ
FLGCKTISRSDFRYNPKNNTLKMLEINTHPGFTELSLVPEIAKLAKGINFNELVKIIIEDSLQHKNIRDLSHVEQYY

Sequences:

>Translated_317_residues
MLMIPTIAILSGGFSCEREISLMSGKAVKKALDSLSYNAIEIDVDSNIAEKLKKINPGLAFIALHGPYGEDGCIQGLLEI
LGIKYTHSGVMASAVAINKVMSKHIFRSLNIDTPKGYVISREDVLKNNIKIDYPYVLKPINEGSSIGVYIIFSHEDYLEL
KDNSSTIMEKMIVEEYIPGIELHTAVLLDEAIGTIEVRPKNKFYDYEAKYTDGFAEHIFPAKIPDNIYKMTLEHALKIHQ
FLGCKTISRSDFRYNPKNNTLKMLEINTHPGFTELSLVPEIAKLAKGINFNELVKIIIEDSLQHKNIRDLSHVEQYY
>Mature_317_residues
MLMIPTIAILSGGFSCEREISLMSGKAVKKALDSLSYNAIEIDVDSNIAEKLKKINPGLAFIALHGPYGEDGCIQGLLEI
LGIKYTHSGVMASAVAINKVMSKHIFRSLNIDTPKGYVISREDVLKNNIKIDYPYVLKPINEGSSIGVYIIFSHEDYLEL
KDNSSTIMEKMIVEEYIPGIELHTAVLLDEAIGTIEVRPKNKFYDYEAKYTDGFAEHIFPAKIPDNIYKMTLEHALKIHQ
FLGCKTISRSDFRYNPKNNTLKMLEINTHPGFTELSLVPEIAKLAKGINFNELVKIIIEDSLQHKNIRDLSHVEQYY

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=308, Percent_Identity=34.7402597402597, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1786579, Length=353, Percent_Identity=28.328611898017, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_WOLWR (C0R5F9)

Other databases:

- EMBL:   CP001391
- RefSeq:   YP_002726792.1
- EnsemblBacteria:   EBWOLT00000001865
- GeneID:   7668904
- GenomeReviews:   CP001391_GR
- KEGG:   wri:WRi_001550
- GeneTree:   EBGT00050000031466
- OMA:   RAFIILH
- ProtClustDB:   PRK01372
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 35728; Mature: 35728

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMIPTIAILSGGFSCEREISLMSGKAVKKALDSLSYNAIEIDVDSNIAEKLKKINPGLA
CEEECEEHHHCCCCCCCHHHHHHCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCEE
FIALHGPYGEDGCIQGLLEILGIKYTHSGVMASAVAINKVMSKHIFRSLNIDTPKGYVIS
EEEEECCCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
REDVLKNNIKIDYPYVLKPINEGSSIGVYIIFSHEDYLELKDNSSTIMEKMIVEEYIPGI
HHHHHHCCCEECCCEEEEECCCCCCEEEEEEEECCCEEEECCCHHHHHHHHHHHHHCCCC
ELHTAVLLDEAIGTIEVRPKNKFYDYEAKYTDGFAEHIFPAKIPDNIYKMTLEHALKIHQ
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHH
FLGCKTISRSDFRYNPKNNTLKMLEINTHPGFTELSLVPEIAKLAKGINFNELVKIIIED
HHCCCCCCCCCCEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
SLQHKNIRDLSHVEQYY
HHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MLMIPTIAILSGGFSCEREISLMSGKAVKKALDSLSYNAIEIDVDSNIAEKLKKINPGLA
CEEECEEHHHCCCCCCCHHHHHHCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCEE
FIALHGPYGEDGCIQGLLEILGIKYTHSGVMASAVAINKVMSKHIFRSLNIDTPKGYVIS
EEEEECCCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
REDVLKNNIKIDYPYVLKPINEGSSIGVYIIFSHEDYLELKDNSSTIMEKMIVEEYIPGI
HHHHHHCCCEECCCEEEEECCCCCCEEEEEEEECCCEEEECCCHHHHHHHHHHHHHCCCC
ELHTAVLLDEAIGTIEVRPKNKFYDYEAKYTDGFAEHIFPAKIPDNIYKMTLEHALKIHQ
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHH
FLGCKTISRSDFRYNPKNNTLKMLEINTHPGFTELSLVPEIAKLAKGINFNELVKIIIED
HHCCCCCCCCCCEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
SLQHKNIRDLSHVEQYY
HHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA