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The map label for this gene is cysS [H]

Identifier: 225629958

GI number: 225629958

Start: 103246

End: 104619

Strand: Direct

Name: cysS [H]

Synonym: WRi_001040

Alternate gene names: 225629958

Gene position: 103246-104619 (Clockwise)

Preceding gene: 225629957

Following gene: 225629959

Centisome position: 7.14

GC content: 33.99

Gene sequence:

>1374_bases
ATGGTTAGGCTTTACAACACCTTAACAAAAAAAAAGGAACTTTTTATACCAATCGATAAAGATCATGTGAAAATGTATGT
TTGCGGACCAACAGTATATGACACAGCACATATAGGCAATGCACGATCTGTTGTTGTGTATGATGTGTTATTTCAGCTAC
TTAAGTTTTGTTATGGCAAAGTTACCTATGTGCGTAACATAACCGACATTGATGATAAGATAATTAATGCAGCAAGTGAG
AAAAATAGCAACATAGAAAGTATAACCACATATTACATTAAAGCTTTTCATGATGATATGGAAAGCATAAATTGTAAAAA
ACCAACATACGAACCAAAAGCAACGGAAAACGTAGATTATATTATAAAATTAATTGAACATTTACTGCAATCAGGCCACG
CTTACGAATCTGACAAACACGTGTATTTTAATATAGAATCTTACCATGAATATGGTGCTTTATCAGGAAAAAAAACTGAT
GAATTGGTTCCAGGTAGCAGAGTTGAAGTTAATGAAAATAAAAAGCACCCTGGAGATTTTGTGCTGTGGAAGCCTGCAAA
TGATATTGACTACAAACTTTCAAGTTATTGGAATAGCCCATGGGGAGAGGGCAGGCCTGGATGGCATATAGAATGCTCAG
CAATGTCATATGCTTACCTTGGCAAAGATTTTGATATTCATGGTGGCGGCATAGATTTACAATTTCCTCACCATGAAAAT
GAAATTGCACAGAGTAAGTCTGCATTTGCTGGATCAATGTTTGCAAAATACTGGATGCATAACGGTTTTCTTACAGTAAA
TGAAGAGAAAATGAGCAAGTCCTTATTTAATATAGTCAAAGTAAGGGATTTGCTAGATAGTGGAATAAAGGGTGAAGTAA
TACGCTATGCACTGCTCAAAACTCACTACAGAAAACCACTTGATTGGACAGAAAACGTTATTTCTGATGCACAGGAAACC
TTAAATAAATTTTATCGATTATCACGTGGTTTAGATACAATAAATATTGATGAAAGTAATGCAGAGATTTCTAAAGATTT
TATAGACGCTTTAAAAAACGACTTAAACATTCCTGAAGCTCTAGCTATATTACATGAAATGGCTGCAAAAATCAACAAAA
TGAGTAATGAAAGTGAAAAGCTTAAATTAACTGAGAGTTTTGTTAAGAGTGCCAGATTTATTGGTCTTCTTGAGTCAAGC
TATCAAGAGTGGTTTACTGCTGGTGTGAGTCATCAAGAAATAGAAAGATTGATAGATTTAAGAAGAGCAGCAAAACAAAA
TAAAGATTATAATACTGCAGACAAAATAAGGGACCAGTTGAAACAAATAGGGGTTACAATTTCTGATAATGAAGATGGTA
CAACAACCTGGTAA

Upstream 100 bases:

>100_bases
GTTCTAATATCATTGTGTGGGTTATTGCTTACTCTTGTTGTAGTAGTCAAAGGGCCACAACTTATAATTGGGGCATTTTC
TTTTCTCAAAAGCTTTGTAT

Downstream 100 bases:

>100_bases
ACTCATATGGAAAACTACTTCCTGTTATTCACAGATAGTCTAGTATCATCATTAGTCTTACCCATACATCAAGGTTTTGT
GTTCAATACCATGCTTTATT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MVRLYNTLTKKKELFIPIDKDHVKMYVCGPTVYDTAHIGNARSVVVYDVLFQLLKFCYGKVTYVRNITDIDDKIINAASE
KNSNIESITTYYIKAFHDDMESINCKKPTYEPKATENVDYIIKLIEHLLQSGHAYESDKHVYFNIESYHEYGALSGKKTD
ELVPGSRVEVNENKKHPGDFVLWKPANDIDYKLSSYWNSPWGEGRPGWHIECSAMSYAYLGKDFDIHGGGIDLQFPHHEN
EIAQSKSAFAGSMFAKYWMHNGFLTVNEEKMSKSLFNIVKVRDLLDSGIKGEVIRYALLKTHYRKPLDWTENVISDAQET
LNKFYRLSRGLDTINIDESNAEISKDFIDALKNDLNIPEALAILHEMAAKINKMSNESEKLKLTESFVKSARFIGLLESS
YQEWFTAGVSHQEIERLIDLRRAAKQNKDYNTADKIRDQLKQIGVTISDNEDGTTTW

Sequences:

>Translated_457_residues
MVRLYNTLTKKKELFIPIDKDHVKMYVCGPTVYDTAHIGNARSVVVYDVLFQLLKFCYGKVTYVRNITDIDDKIINAASE
KNSNIESITTYYIKAFHDDMESINCKKPTYEPKATENVDYIIKLIEHLLQSGHAYESDKHVYFNIESYHEYGALSGKKTD
ELVPGSRVEVNENKKHPGDFVLWKPANDIDYKLSSYWNSPWGEGRPGWHIECSAMSYAYLGKDFDIHGGGIDLQFPHHEN
EIAQSKSAFAGSMFAKYWMHNGFLTVNEEKMSKSLFNIVKVRDLLDSGIKGEVIRYALLKTHYRKPLDWTENVISDAQET
LNKFYRLSRGLDTINIDESNAEISKDFIDALKNDLNIPEALAILHEMAAKINKMSNESEKLKLTESFVKSARFIGLLESS
YQEWFTAGVSHQEIERLIDLRRAAKQNKDYNTADKIRDQLKQIGVTISDNEDGTTTW
>Mature_457_residues
MVRLYNTLTKKKELFIPIDKDHVKMYVCGPTVYDTAHIGNARSVVVYDVLFQLLKFCYGKVTYVRNITDIDDKIINAASE
KNSNIESITTYYIKAFHDDMESINCKKPTYEPKATENVDYIIKLIEHLLQSGHAYESDKHVYFNIESYHEYGALSGKKTD
ELVPGSRVEVNENKKHPGDFVLWKPANDIDYKLSSYWNSPWGEGRPGWHIECSAMSYAYLGKDFDIHGGGIDLQFPHHEN
EIAQSKSAFAGSMFAKYWMHNGFLTVNEEKMSKSLFNIVKVRDLLDSGIKGEVIRYALLKTHYRKPLDWTENVISDAQET
LNKFYRLSRGLDTINIDESNAEISKDFIDALKNDLNIPEALAILHEMAAKINKMSNESEKLKLTESFVKSARFIGLLESS
YQEWFTAGVSHQEIERLIDLRRAAKQNKDYNTADKIRDQLKQIGVTISDNEDGTTTW

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI13375695, Length=512, Percent_Identity=32.2265625, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI21269877, Length=311, Percent_Identity=32.475884244373, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI10835051, Length=311, Percent_Identity=32.475884244373, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI62240992, Length=311, Percent_Identity=32.475884244373, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI303304987, Length=311, Percent_Identity=32.475884244373, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1786737, Length=463, Percent_Identity=42.3326133909287, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17509797, Length=270, Percent_Identity=31.8518518518519, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17509795, Length=270, Percent_Identity=31.8518518518519, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6324082, Length=318, Percent_Identity=33.9622641509434, Blast_Score=169, Evalue=9e-43,
Organism=Drosophila melanogaster, GI19922218, Length=505, Percent_Identity=30.8910891089109, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI19922364, Length=275, Percent_Identity=36.7272727272727, Blast_Score=178, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e [H]

EC number: =6.1.1.16 [H]

Molecular weight: Translated: 52468; Mature: 52468

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRLYNTLTKKKELFIPIDKDHVKMYVCGPTVYDTAHIGNARSVVVYDVLFQLLKFCYGK
CEEHHHHHCCCCCEEEEECCCCEEEEEECCCEECHHCCCCCCHHHHHHHHHHHHHHHHCC
VTYVRNITDIDDKIINAASEKNSNIESITTYYIKAFHDDMESINCKKPTYEPKATENVDY
HHEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
IIKLIEHLLQSGHAYESDKHVYFNIESYHEYGALSGKKTDELVPGSRVEVNENKKHPGDF
HHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCE
VLWKPANDIDYKLSSYWNSPWGEGRPGWHIECSAMSYAYLGKDFDIHGGGIDLQFPHHEN
EEECCCCCCCHHHHHHCCCCCCCCCCCCEEEECCEEHHHHCCCEEECCCCEEEECCCCHH
EIAQSKSAFAGSMFAKYWMHNGFLTVNEEKMSKSLFNIVKVRDLLDSGIKGEVIRYALLK
HHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
THYRKPLDWTENVISDAQETLNKFYRLSRGLDTINIDESNAEISKDFIDALKNDLNIPEA
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCHHH
LAILHEMAAKINKMSNESEKLKLTESFVKSARFIGLLESSYQEWFTAGVSHQEIERLIDL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RRAAKQNKDYNTADKIRDQLKQIGVTISDNEDGTTTW
HHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCC
>Mature Secondary Structure
MVRLYNTLTKKKELFIPIDKDHVKMYVCGPTVYDTAHIGNARSVVVYDVLFQLLKFCYGK
CEEHHHHHCCCCCEEEEECCCCEEEEEECCCEECHHCCCCCCHHHHHHHHHHHHHHHHCC
VTYVRNITDIDDKIINAASEKNSNIESITTYYIKAFHDDMESINCKKPTYEPKATENVDY
HHEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
IIKLIEHLLQSGHAYESDKHVYFNIESYHEYGALSGKKTDELVPGSRVEVNENKKHPGDF
HHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCE
VLWKPANDIDYKLSSYWNSPWGEGRPGWHIECSAMSYAYLGKDFDIHGGGIDLQFPHHEN
EEECCCCCCCHHHHHHCCCCCCCCCCCCEEEECCEEHHHHCCCEEECCCCEEEECCCCHH
EIAQSKSAFAGSMFAKYWMHNGFLTVNEEKMSKSLFNIVKVRDLLDSGIKGEVIRYALLK
HHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
THYRKPLDWTENVISDAQETLNKFYRLSRGLDTINIDESNAEISKDFIDALKNDLNIPEA
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCHHH
LAILHEMAAKINKMSNESEKLKLTESFVKSARFIGLLESSYQEWFTAGVSHQEIERLIDL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RRAAKQNKDYNTADKIRDQLKQIGVTISDNEDGTTTW
HHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA