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The map label for this gene is rlmE [H]

Identifier: 225629924

GI number: 225629924

Start: 64762

End: 65340

Strand: Direct

Name: rlmE [H]

Synonym: WRi_000610

Alternate gene names: 225629924

Gene position: 64762-65340 (Clockwise)

Preceding gene: 225629923

Following gene: 225629925

Centisome position: 4.48

GC content: 33.33

Gene sequence:

>579_bases
TTGAATGATCAGTATGTGCAAAAAACTAGCAAGGATGGTTATAGGTCACGCTCAGCATATAAGCTGGTAGAAATGGATAA
CAAATTTAAATTATTCCAAGAAGGGCAAAAAATTATTGATCTTGGTGCTTCTCCTGGTGGATGGTTACAAGTTGCATCTC
AGAAAGGTGCAAATGTGGTTGCTCTTGACGTAAAACCAATGAACGCAATTAATGGAGTAGAGTTTATACAATGTGATATT
ATCAATGAGTTTGAAATTTTAAGAGAAAAATTCAAGGATCAAAAATTTGATGTAATTTTATCTGATATGGCGCCCGAATC
TTGTGGTTTAAAATCGTTGGATCATATCAGAATTATGCTTTTATGTGAGGCAGCGCTCAATTTTGCAAAGCATTTCTTGA
GCCATGGTGGCACGTTTGTAGTAAAAATTTTCCAAGGAGAATCTGATAAAGATTTTTGTAACGAGCTAAAAAAAATGTTT
AAAACAGTAAAATACTTTAAACCAAAGTCAAGTAGATCTGAATCTACAGAAATGTATTTGGTAAGTTTAGGCTTTATTGG
CAGCAGACTTTCTATATAA

Upstream 100 bases:

>100_bases
AAAGATATAATCAATAATAAGTAGGGTATTAGCAATAAAAACTAGAGTAAAGACGGCTAGAGGCAGAAAACTATCTTCAA
CAAGGTGGCTACATCGTCAT

Downstream 100 bases:

>100_bases
TATTAATCTAGGTAGTTCGCAGAAACAAAAAACTTACTTGACAAACTCCGCCAGCCCACTTATCATGAAACTGAAGCTAT
TGTATTTGCTTTCGCCAATC

Product: ribosomal RNA large subunit methyltransferase J, putative

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MNDQYVQKTSKDGYRSRSAYKLVEMDNKFKLFQEGQKIIDLGASPGGWLQVASQKGANVVALDVKPMNAINGVEFIQCDI
INEFEILREKFKDQKFDVILSDMAPESCGLKSLDHIRIMLLCEAALNFAKHFLSHGGTFVVKIFQGESDKDFCNELKKMF
KTVKYFKPKSSRSESTEMYLVSLGFIGSRLSI

Sequences:

>Translated_192_residues
MNDQYVQKTSKDGYRSRSAYKLVEMDNKFKLFQEGQKIIDLGASPGGWLQVASQKGANVVALDVKPMNAINGVEFIQCDI
INEFEILREKFKDQKFDVILSDMAPESCGLKSLDHIRIMLLCEAALNFAKHFLSHGGTFVVKIFQGESDKDFCNELKKMF
KTVKYFKPKSSRSESTEMYLVSLGFIGSRLSI
>Mature_192_residues
MNDQYVQKTSKDGYRSRSAYKLVEMDNKFKLFQEGQKIIDLGASPGGWLQVASQKGANVVALDVKPMNAINGVEFIQCDI
INEFEILREKFKDQKFDVILSDMAPESCGLKSLDHIRIMLLCEAALNFAKHFLSHGGTFVVKIFQGESDKDFCNELKKMF
KTVKYFKPKSSRSESTEMYLVSLGFIGSRLSI

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI29029591, Length=191, Percent_Identity=38.2198952879581, Blast_Score=127, Evalue=6e-30,
Organism=Homo sapiens, GI29029589, Length=191, Percent_Identity=38.2198952879581, Blast_Score=127, Evalue=6e-30,
Organism=Homo sapiens, GI7110661, Length=191, Percent_Identity=38.2198952879581, Blast_Score=127, Evalue=6e-30,
Organism=Homo sapiens, GI7019377, Length=207, Percent_Identity=31.8840579710145, Blast_Score=125, Evalue=2e-29,
Organism=Homo sapiens, GI194097365, Length=190, Percent_Identity=34.2105263157895, Blast_Score=118, Evalue=3e-27,
Organism=Escherichia coli, GI1789569, Length=188, Percent_Identity=43.6170212765958, Blast_Score=159, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17553474, Length=193, Percent_Identity=34.7150259067358, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17554650, Length=192, Percent_Identity=34.375, Blast_Score=121, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71987550, Length=185, Percent_Identity=35.6756756756757, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71987561, Length=185, Percent_Identity=35.6756756756757, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17553860, Length=185, Percent_Identity=35.6756756756757, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=34.3434343434343, Blast_Score=118, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321302, Length=270, Percent_Identity=27.037037037037, Blast_Score=95, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319796, Length=190, Percent_Identity=34.7368421052632, Blast_Score=93, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356387, Length=202, Percent_Identity=32.6732673267327, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648639, Length=197, Percent_Identity=31.9796954314721, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI18859957, Length=190, Percent_Identity=34.7368421052632, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=31.3131313131313, Blast_Score=106, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 21758; Mature: 21758

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDQYVQKTSKDGYRSRSAYKLVEMDNKFKLFQEGQKIIDLGASPGGWLQVASQKGANVV
CCCHHHHHCCCCCCCCCCCEEEECCCCCHHHHHCCCCEEECCCCCCCCEEEECCCCCEEE
ALDVKPMNAINGVEFIQCDIINEFEILREKFKDQKFDVILSDMAPESCGLKSLDHIRIML
EEECCCCCCCCCCEEEEEECCHHHHHHHHHHCCCHHEEHHHCCCCCCCCCCCHHHHHHHH
LCEAALNFAKHFLSHGGTFVVKIFQGESDKDFCNELKKMFKTVKYFKPKSSRSESTEMYL
HHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
VSLGFIGSRLSI
EEECCCCCCCCC
>Mature Secondary Structure
MNDQYVQKTSKDGYRSRSAYKLVEMDNKFKLFQEGQKIIDLGASPGGWLQVASQKGANVV
CCCHHHHHCCCCCCCCCCCEEEECCCCCHHHHHCCCCEEECCCCCCCCEEEECCCCCEEE
ALDVKPMNAINGVEFIQCDIINEFEILREKFKDQKFDVILSDMAPESCGLKSLDHIRIML
EEECCCCCCCCCCEEEEEECCHHHHHHHHHHCCCHHEEHHHCCCCCCCCCCCHHHHHHHH
LCEAALNFAKHFLSHGGTFVVKIFQGESDKDFCNELKKMFKTVKYFKPKSSRSESTEMYL
HHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
VSLGFIGSRLSI
EEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA