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The map label for this gene is leuS [H]

Identifier: 225629915

GI number: 225629915

Start: 54797

End: 57313

Strand: Direct

Name: leuS [H]

Synonym: WRi_000480

Alternate gene names: 225629915

Gene position: 54797-57313 (Clockwise)

Preceding gene: 225629914

Following gene: 225629916

Centisome position: 3.79

GC content: 38.62

Gene sequence:

>2517_bases
ATGAAATATGATTTTAAAAACGTCGAAAGGTTTTGTCAAGATAAATGGGATTTTTCTGTAAGCAAGAGTAGTAAACAAGA
GAAATGTTACGTATTGGAGATGTTTCCATATCCATCTGGTAAAATTCATATGGGGCACTTACGTAACTATGCAATAGGGG
ATGTGATAGCGCGTTACAAGAGAGCTCGTGGATTTGAGGTTTTGCATCCAATTGGCTGGGATGCGTTTGGATTACCAGCT
GAAAATGCAGCAAGGGACAACAATATTAGCCCTGAAATATGGACAAAAGAGAATATAGATAATATGCGTGCACAGTTGAA
ATCTATAGGTCTTTCTTATAATTGGAACCGTGAACTTTCCACGTGTGAGCCTAATTATTACACACATGAACAAAAATTTT
TCCTGGATTTTTTAAAGCATGGGCTTGCCTATCGAAAAGAGTCATGGGTTAACTGGGACCCGGTAGACCAAACAGTGCTT
GCAAATGAACAGGTGGTTGATGGAAAAGGATGGCGATCAGGTGCAGTTGTTGAAAAACGTAAGTTATCCCAATGGTTTTT
AAAGATTACTGATTTTGCTGAAGATTTACTCAAGTGCCTGCAAAGTTTGAAAAATTGGCCAGAAAAAGTCAAAACAATGC
AGGAGCGTTGGATAGGAAAATCTGAAGGAGCAACTATAGAGTTTGAAGTAGTTGGCTTAAATAAGAAATTGAAGGTTTTT
ACAACTTATCCTCATACTTTGTTTGGGGCTTCTTTTTGTGCAGTGGCAGCAGAGCATCCTATTGTGCAGGATTTAAAGAA
TGGTTCTTCTGTTGTCATCCCAGTGCTTGACACTGGGATCCAGGAAATAAAATCGAAAAGAGAGAACGATGAAAAAATAG
GAGTTTACACCGGATTAAACGTCAAACATCCATTTCTTGATAAGGAATTGCCGCTTTACATAGCGAATTTTGTGTTGATG
GAATATGGGGAAGGCGCGATTTTTGGTTGCCCTGCACATGATCAGCGTGACTTTGAATTTGCACAGAAATATAATTTGCC
GATAATTCCCGTTATTTCTTCAGCCCACTTAGGTGTCATTCCAGCGCGTGACCAGAATTCTTACAATGGATCCCAGTGTC
AAGCTACTCAGATGACAAAGGAGGCGTATACTGGTGATGGGGTAATGTTTAACTCCGAATTCTTAAACGGATTAATGGTT
AGTGAAGCAAAGGAAGTAATTATTAAAAAGCTCAAAGAAAAAGGAATAGGCAAGAAAACAACAAACTATCGTTTGCACGA
TTGGGGAGTTTCAAGACAGCGTTATTGGGGATGCCCTATACCTATCATATATTGCAAAGACTGTGGTACTGTTCCAGTGC
CAGAAAAAGACCTGCCTGTAATACTGCCCGCGGATGTGGAATTCACAAGTGGTGGCAATCCACTTGATAAACATCCTACT
TGGAAATTTGTTGATTGCCCAAAATGTGGAAAGCAAGCAGAGCGTGAAACTGATACGTTTGACACCTTTTTTGAATCCTC
TTGGTATTTTGCTGCATTCTGTAGCGAAGATAAATCAATAGACAAAGATGCGTGTAATCGTTTTATGCCTGTTGATTATT
ATATAGGTGGAATAGAGCATGCAATTCTGCATTTGCTTTACTCACGATTTTTCTGCCGTGCTTTAACCAAATGTGGCTAT
TTTGATATTAAAGAGCCTTTCTCTACTTTAATCACTCAAGGAATGGTCTGCCATGCAACTTATAAAGACGAAAATGGTAA
GTGGCTATTTCTCGCAGAAGCAAAAGAATTAATAGCGCGAGGTACAAAAGTTCAAGTGGGCAAAGTTGAAAAAATGAGCA
AATCGAAAAAGAATACAGTTGACCCAAACTTTATCATAGAAAAGTATGGTGCTGATACTGCTCGCTTGTTTGTGTTGTCT
GACACTCCTCCAGAGAAAGATATGGAATGGTCCGATGATGGTGTGGAAGGTTGTTCTCGTTATGTAAATAAATTGTGGCG
TATGGTCATGCAGCTTAAGCCTGTGAATATGCACTATGATAACAAAAGTGTTACGGGTGGACTTCTAGAGTACAGAAAAA
AAATTCATAAACTCTTACATGGACTTACAGATGACTTGGAAAACTGCAGATTAAACTGTGTAGTGGCAAAATTTCGTGAA
ATGACGAATTTAATAGCTGAAATAGACGTAGCAGCTGGAAAATCTCTTATTGATGAAGGTATATGCATACTAATCAGAGT
AATCGAACCATTTATGCCGCATTTAGCTGAAAGCCTATGGCAAGAAATAGGAGGTCAACCTTGGCCAAAAGCTGATGAAT
CATTATTAGTTGACGATACGGTAACTATAGCAGTGCAGATCAATGGAAAGTTGCGTGCGACAATCGAAGTGGCAATTAAC
TTACCTCAAGAAGAATTGAAGAAAATAGCGATAGACTCTGTGTCCAGTAAGATCGATCAAAACAAAGTTCGCACTGTATA
TGCTGTACCAAATAAGATAGTAAATATAGTTATATAA

Upstream 100 bases:

>100_bases
CACACAAGATAAAGACCCGCAGATGTGTTTCACAATACGGCATAATGGCCAAGCGGTTGATCCTTTGGCGCACACAAACT
GTGATTAAATTTGGATTTAT

Downstream 100 bases:

>100_bases
AAAATCCGTAGCGCATCTTTGACTTCATGGTTATACTCTTTTTAACATGCGATTATATCTTTTTTAATATAAAGGATATA
TACTTATGCATTATATATTT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 838; Mature: 838

Protein sequence:

>838_residues
MKYDFKNVERFCQDKWDFSVSKSSKQEKCYVLEMFPYPSGKIHMGHLRNYAIGDVIARYKRARGFEVLHPIGWDAFGLPA
ENAARDNNISPEIWTKENIDNMRAQLKSIGLSYNWNRELSTCEPNYYTHEQKFFLDFLKHGLAYRKESWVNWDPVDQTVL
ANEQVVDGKGWRSGAVVEKRKLSQWFLKITDFAEDLLKCLQSLKNWPEKVKTMQERWIGKSEGATIEFEVVGLNKKLKVF
TTYPHTLFGASFCAVAAEHPIVQDLKNGSSVVIPVLDTGIQEIKSKRENDEKIGVYTGLNVKHPFLDKELPLYIANFVLM
EYGEGAIFGCPAHDQRDFEFAQKYNLPIIPVISSAHLGVIPARDQNSYNGSQCQATQMTKEAYTGDGVMFNSEFLNGLMV
SEAKEVIIKKLKEKGIGKKTTNYRLHDWGVSRQRYWGCPIPIIYCKDCGTVPVPEKDLPVILPADVEFTSGGNPLDKHPT
WKFVDCPKCGKQAERETDTFDTFFESSWYFAAFCSEDKSIDKDACNRFMPVDYYIGGIEHAILHLLYSRFFCRALTKCGY
FDIKEPFSTLITQGMVCHATYKDENGKWLFLAEAKELIARGTKVQVGKVEKMSKSKKNTVDPNFIIEKYGADTARLFVLS
DTPPEKDMEWSDDGVEGCSRYVNKLWRMVMQLKPVNMHYDNKSVTGGLLEYRKKIHKLLHGLTDDLENCRLNCVVAKFRE
MTNLIAEIDVAAGKSLIDEGICILIRVIEPFMPHLAESLWQEIGGQPWPKADESLLVDDTVTIAVQINGKLRATIEVAIN
LPQEELKKIAIDSVSSKIDQNKVRTVYAVPNKIVNIVI

Sequences:

>Translated_838_residues
MKYDFKNVERFCQDKWDFSVSKSSKQEKCYVLEMFPYPSGKIHMGHLRNYAIGDVIARYKRARGFEVLHPIGWDAFGLPA
ENAARDNNISPEIWTKENIDNMRAQLKSIGLSYNWNRELSTCEPNYYTHEQKFFLDFLKHGLAYRKESWVNWDPVDQTVL
ANEQVVDGKGWRSGAVVEKRKLSQWFLKITDFAEDLLKCLQSLKNWPEKVKTMQERWIGKSEGATIEFEVVGLNKKLKVF
TTYPHTLFGASFCAVAAEHPIVQDLKNGSSVVIPVLDTGIQEIKSKRENDEKIGVYTGLNVKHPFLDKELPLYIANFVLM
EYGEGAIFGCPAHDQRDFEFAQKYNLPIIPVISSAHLGVIPARDQNSYNGSQCQATQMTKEAYTGDGVMFNSEFLNGLMV
SEAKEVIIKKLKEKGIGKKTTNYRLHDWGVSRQRYWGCPIPIIYCKDCGTVPVPEKDLPVILPADVEFTSGGNPLDKHPT
WKFVDCPKCGKQAERETDTFDTFFESSWYFAAFCSEDKSIDKDACNRFMPVDYYIGGIEHAILHLLYSRFFCRALTKCGY
FDIKEPFSTLITQGMVCHATYKDENGKWLFLAEAKELIARGTKVQVGKVEKMSKSKKNTVDPNFIIEKYGADTARLFVLS
DTPPEKDMEWSDDGVEGCSRYVNKLWRMVMQLKPVNMHYDNKSVTGGLLEYRKKIHKLLHGLTDDLENCRLNCVVAKFRE
MTNLIAEIDVAAGKSLIDEGICILIRVIEPFMPHLAESLWQEIGGQPWPKADESLLVDDTVTIAVQINGKLRATIEVAIN
LPQEELKKIAIDSVSSKIDQNKVRTVYAVPNKIVNIVI
>Mature_838_residues
MKYDFKNVERFCQDKWDFSVSKSSKQEKCYVLEMFPYPSGKIHMGHLRNYAIGDVIARYKRARGFEVLHPIGWDAFGLPA
ENAARDNNISPEIWTKENIDNMRAQLKSIGLSYNWNRELSTCEPNYYTHEQKFFLDFLKHGLAYRKESWVNWDPVDQTVL
ANEQVVDGKGWRSGAVVEKRKLSQWFLKITDFAEDLLKCLQSLKNWPEKVKTMQERWIGKSEGATIEFEVVGLNKKLKVF
TTYPHTLFGASFCAVAAEHPIVQDLKNGSSVVIPVLDTGIQEIKSKRENDEKIGVYTGLNVKHPFLDKELPLYIANFVLM
EYGEGAIFGCPAHDQRDFEFAQKYNLPIIPVISSAHLGVIPARDQNSYNGSQCQATQMTKEAYTGDGVMFNSEFLNGLMV
SEAKEVIIKKLKEKGIGKKTTNYRLHDWGVSRQRYWGCPIPIIYCKDCGTVPVPEKDLPVILPADVEFTSGGNPLDKHPT
WKFVDCPKCGKQAERETDTFDTFFESSWYFAAFCSEDKSIDKDACNRFMPVDYYIGGIEHAILHLLYSRFFCRALTKCGY
FDIKEPFSTLITQGMVCHATYKDENGKWLFLAEAKELIARGTKVQVGKVEKMSKSKKNTVDPNFIIEKYGADTARLFVLS
DTPPEKDMEWSDDGVEGCSRYVNKLWRMVMQLKPVNMHYDNKSVTGGLLEYRKKIHKLLHGLTDDLENCRLNCVVAKFRE
MTNLIAEIDVAAGKSLIDEGICILIRVIEPFMPHLAESLWQEIGGQPWPKADESLLVDDTVTIAVQINGKLRATIEVAIN
LPQEELKKIAIDSVSSKIDQNKVRTVYAVPNKIVNIVI

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=859, Percent_Identity=34.2258440046566, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI5454158, Length=648, Percent_Identity=23.3024691358025, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786861, Length=874, Percent_Identity=42.5629290617849, Blast_Score=706, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=372, Percent_Identity=22.8494623655914, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71997517, Length=194, Percent_Identity=50.5154639175258, Blast_Score=216, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI71997510, Length=194, Percent_Identity=50.5154639175258, Blast_Score=216, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI212645227, Length=373, Percent_Identity=28.4182305630027, Blast_Score=127, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323414, Length=837, Percent_Identity=39.0681003584229, Blast_Score=552, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6321531, Length=899, Percent_Identity=21.579532814238, Blast_Score=137, Evalue=6e-33,
Organism=Drosophila melanogaster, GI21355409, Length=871, Percent_Identity=32.3765786452354, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI281366294, Length=793, Percent_Identity=20.9331651954603, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 95499; Mature: 95499

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDFKNVERFCQDKWDFSVSKSSKQEKCYVLEMFPYPSGKIHMGHLRNYAIGDVIARYK
CCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEHHHHCCHHHHHHHHHHH
RARGFEVLHPIGWDAFGLPAENAARDNNISPEIWTKENIDNMRAQLKSIGLSYNWNRELS
HHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
TCEPNYYTHEQKFFLDFLKHGLAYRKESWVNWDPVDQTVLANEQVVDGKGWRSGAVVEKR
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEECCCCCCCCCHHHHH
KLSQWFLKITDFAEDLLKCLQSLKNWPEKVKTMQERWIGKSEGATIEFEVVGLNKKLKVF
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEE
TTYPHTLFGASFCAVAAEHPIVQDLKNGSSVVIPVLDTGIQEIKSKRENDEKIGVYTGLN
EECCHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEECCC
VKHPFLDKELPLYIANFVLMEYGEGAIFGCPAHDQRDFEFAQKYNLPIIPVISSAHLGVI
CCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCCEEEEECCCCEEEE
PARDQNSYNGSQCQATQMTKEAYTGDGVMFNSEFLNGLMVSEAKEVIIKKLKEKGIGKKT
ECCCCCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TNYRLHDWGVSRQRYWGCPIPIIYCKDCGTVPVPEKDLPVILPADVEFTSGGNPLDKHPT
CCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEECCCEEECCCCCCCCCCCC
WKFVDCPKCGKQAERETDTFDTFFESSWYFAAFCSEDKSIDKDACNRFMPVDYYIGGIEH
EEEEECCHHCHHHHCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHCHHHH
AILHLLYSRFFCRALTKCGYFDIKEPFSTLITQGMVCHATYKDENGKWLFLAEAKELIAR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEEEECCCCCEEEEECHHHHHHC
GTKVQVGKVEKMSKSKKNTVDPNFIIEKYGADTARLFVLSDTPPEKDMEWSDDGVEGCSR
CCEEEECCHHHHHHCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHH
YVNKLWRMVMQLKPVNMHYDNKSVTGGLLEYRKKIHKLLHGLTDDLENCRLNCVVAKFRE
HHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEHHHHHHHHH
MTNLIAEIDVAAGKSLIDEGICILIRVIEPFMPHLAESLWQEIGGQPWPKADESLLVDDT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECE
VTIAVQINGKLRATIEVAINLPQEELKKIAIDSVSSKIDQNKVRTVYAVPNKIVNIVI
EEEEEEECCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEEEEC
>Mature Secondary Structure
MKYDFKNVERFCQDKWDFSVSKSSKQEKCYVLEMFPYPSGKIHMGHLRNYAIGDVIARYK
CCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEHHHHCCHHHHHHHHHHH
RARGFEVLHPIGWDAFGLPAENAARDNNISPEIWTKENIDNMRAQLKSIGLSYNWNRELS
HHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
TCEPNYYTHEQKFFLDFLKHGLAYRKESWVNWDPVDQTVLANEQVVDGKGWRSGAVVEKR
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEECCCCCCCCCHHHHH
KLSQWFLKITDFAEDLLKCLQSLKNWPEKVKTMQERWIGKSEGATIEFEVVGLNKKLKVF
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEE
TTYPHTLFGASFCAVAAEHPIVQDLKNGSSVVIPVLDTGIQEIKSKRENDEKIGVYTGLN
EECCHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEECCC
VKHPFLDKELPLYIANFVLMEYGEGAIFGCPAHDQRDFEFAQKYNLPIIPVISSAHLGVI
CCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCCEEEEECCCCEEEE
PARDQNSYNGSQCQATQMTKEAYTGDGVMFNSEFLNGLMVSEAKEVIIKKLKEKGIGKKT
ECCCCCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TNYRLHDWGVSRQRYWGCPIPIIYCKDCGTVPVPEKDLPVILPADVEFTSGGNPLDKHPT
CCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEECCCEEECCCCCCCCCCCC
WKFVDCPKCGKQAERETDTFDTFFESSWYFAAFCSEDKSIDKDACNRFMPVDYYIGGIEH
EEEEECCHHCHHHHCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHCHHHH
AILHLLYSRFFCRALTKCGYFDIKEPFSTLITQGMVCHATYKDENGKWLFLAEAKELIAR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEEEECCCCCEEEEECHHHHHHC
GTKVQVGKVEKMSKSKKNTVDPNFIIEKYGADTARLFVLSDTPPEKDMEWSDDGVEGCSR
CCEEEECCHHHHHHCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHH
YVNKLWRMVMQLKPVNMHYDNKSVTGGLLEYRKKIHKLLHGLTDDLENCRLNCVVAKFRE
HHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEHHHHHHHHH
MTNLIAEIDVAAGKSLIDEGICILIRVIEPFMPHLAESLWQEIGGQPWPKADESLLVDDT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECE
VTIAVQINGKLRATIEVAINLPQEELKKIAIDSVSSKIDQNKVRTVYAVPNKIVNIVI
EEEEEEECCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA