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The map label for this gene is echA12 [H]

Identifier: 222527137

GI number: 222527137

Start: 4868265

End: 4869053

Strand: Direct

Name: echA12 [H]

Synonym: Chy400_3919

Alternate gene names: 222527137

Gene position: 4868265-4869053 (Clockwise)

Preceding gene: 222527136

Following gene: 222527138

Centisome position: 92.4

GC content: 58.94

Gene sequence:

>789_bases
ATGAGTAATCTGGTAACCATTCTAACAAACGGCCCGGTTGTAACTCTCACCCTTAACCGGCCGATGCAGCACAACGGCCT
GACGCCAGAGCTGTTAACCGAGCTGGAAGCGCACATTATTGCAGCACGTCAGCATCATGGACTACGGGCTATCGTCTTGC
AAGCCAGTGGCACCTATTTTTCGTGCGGGACTGACCTGGTAGCCCTGGTCCGGCAAACCGACCGGCTCGCTTACGCCGAT
CATGGGTTGCGCCTGTTGCATCAGGTGATGTTGACGCTGATAGATCTGCCGGTGCCGTTCATTGTCGCTGTGCAGGGACC
GGTGAACGGAAGCGCGCTGGGGCTGGTTGTGGTGGCTGACATCGTCCTGGTGACACCCAATGCCCATTTCACGGCCTACG
CGACAACCCTCGGTCTGTCGCCGGAAGGAGGGTGGACGACGCTCTTGCCACGACTGATCGGCCAACGACGTGCCGGCGAA
GTGTTACTTCTCGAACGGCCGATCACGGCCGAGGAGGCAGTGGCCTGGGGATTAGCAAACCGCCTGGTCGCGCCCGGCCA
TCTTTTTGACGAAGCCCAGGCCCTGGCCTACCAGATTGCAACGCAGATGCCGGGTAGTGTCGCCTGCACACGCCGGTTGC
TCTGGGCGGAACGGGAACAGATCGCTGCCGCCCTCAATCGTGAACGAGAAGTGTATTGCGAGCGTATTCAGTCGATTGAG
GCTGATCTTGGCTTGAAAGCCTTTCTCGACCGGCAACGCACAACAATGACCACGGAGGAAACGGTATGA

Upstream 100 bases:

>100_bases
GTGACCGGTAAGGACGATAACACGATCAATTGCACGGTCAACGTCACCCGTGCTGACGGTAAGGTCGCCTGTAGTGGTGA
GACAAGATTGATTGTTGGGC

Downstream 100 bases:

>100_bases
CAGCGCTGACCGTTGGAATGCAGGCGACCGTCCAGCGCGTGATCGATCAAGCCCTGATTGACGAATATGCACGGCTCTGC
GATGACCAACCGCAAAAGAC

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSNLVTILTNGPVVTLTLNRPMQHNGLTPELLTELEAHIIAARQHHGLRAIVLQASGTYFSCGTDLVALVRQTDRLAYAD
HGLRLLHQVMLTLIDLPVPFIVAVQGPVNGSALGLVVVADIVLVTPNAHFTAYATTLGLSPEGGWTTLLPRLIGQRRAGE
VLLLERPITAEEAVAWGLANRLVAPGHLFDEAQALAYQIATQMPGSVACTRRLLWAEREQIAAALNREREVYCERIQSIE
ADLGLKAFLDRQRTTMTTEETV

Sequences:

>Translated_262_residues
MSNLVTILTNGPVVTLTLNRPMQHNGLTPELLTELEAHIIAARQHHGLRAIVLQASGTYFSCGTDLVALVRQTDRLAYAD
HGLRLLHQVMLTLIDLPVPFIVAVQGPVNGSALGLVVVADIVLVTPNAHFTAYATTLGLSPEGGWTTLLPRLIGQRRAGE
VLLLERPITAEEAVAWGLANRLVAPGHLFDEAQALAYQIATQMPGSVACTRRLLWAEREQIAAALNREREVYCERIQSIE
ADLGLKAFLDRQRTTMTTEETV
>Mature_261_residues
SNLVTILTNGPVVTLTLNRPMQHNGLTPELLTELEAHIIAARQHHGLRAIVLQASGTYFSCGTDLVALVRQTDRLAYADH
GLRLLHQVMLTLIDLPVPFIVAVQGPVNGSALGLVVVADIVLVTPNAHFTAYATTLGLSPEGGWTTLLPRLIGQRRAGEV
LLLERPITAEEAVAWGLANRLVAPGHLFDEAQALAYQIATQMPGSVACTRRLLWAEREQIAAALNREREVYCERIQSIEA
DLGLKAFLDRQRTTMTTEETV

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI221136756, Length=219, Percent_Identity=28.310502283105, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI221136753, Length=219, Percent_Identity=28.310502283105, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI221307494, Length=222, Percent_Identity=27.9279279279279, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI45643119, Length=243, Percent_Identity=26.7489711934156, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI260275230, Length=243, Percent_Identity=26.7489711934156, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI260274832, Length=243, Percent_Identity=26.7489711934156, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4757968, Length=174, Percent_Identity=28.1609195402299, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI49355787, Length=174, Percent_Identity=28.1609195402299, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI25453481, Length=222, Percent_Identity=25.6756756756757, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI51538573, Length=222, Percent_Identity=25.6756756756757, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4757966, Length=222, Percent_Identity=25.6756756756757, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI51538581, Length=222, Percent_Identity=25.6756756756757, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI70995211, Length=226, Percent_Identity=28.3185840707965, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=24.8, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=25.4980079681275, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI116235487, Length=165, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI221142681, Length=215, Percent_Identity=28.8372093023256, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=32.5396825396825, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1787659, Length=241, Percent_Identity=28.2157676348548, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=26.1538461538462, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=26.8774703557312, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=28.3400809716599, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=261, Percent_Identity=24.5210727969349, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=242, Percent_Identity=26.0330578512397, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=245, Percent_Identity=25.3061224489796, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=28.6290322580645, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=28.6290322580645, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24654903, Length=178, Percent_Identity=24.7191011235955, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19920382, Length=214, Percent_Identity=29.4392523364486, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=24.0157480314961, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=24.1106719367589, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28592; Mature: 28460

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLVTILTNGPVVTLTLNRPMQHNGLTPELLTELEAHIIAARQHHGLRAIVLQASGTYF
CCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEE
SCGTDLVALVRQTDRLAYADHGLRLLHQVMLTLIDLPVPFIVAVQGPVNGSALGLVVVAD
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHEE
IVLVTPNAHFTAYATTLGLSPEGGWTTLLPRLIGQRRAGEVLLLERPITAEEAVAWGLAN
EHEECCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
RLVAPGHLFDEAQALAYQIATQMPGSVACTRRLLWAEREQIAAALNREREVYCERIQSIE
HCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
ADLGLKAFLDRQRTTMTTEETV
HCCCHHHHHCCHHHCCCCCCCC
>Mature Secondary Structure 
SNLVTILTNGPVVTLTLNRPMQHNGLTPELLTELEAHIIAARQHHGLRAIVLQASGTYF
CCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEE
SCGTDLVALVRQTDRLAYADHGLRLLHQVMLTLIDLPVPFIVAVQGPVNGSALGLVVVAD
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHEE
IVLVTPNAHFTAYATTLGLSPEGGWTTLLPRLIGQRRAGEVLLLERPITAEEAVAWGLAN
EHEECCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
RLVAPGHLFDEAQALAYQIATQMPGSVACTRRLLWAEREQIAAALNREREVYCERIQSIE
HCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
ADLGLKAFLDRQRTTMTTEETV
HCCCHHHHHCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]