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The map label for this gene is pfpI [H]

Identifier: 222526589

GI number: 222526589

Start: 4183426

End: 4184031

Strand: Direct

Name: pfpI [H]

Synonym: Chy400_3357

Alternate gene names: 222526589

Gene position: 4183426-4184031 (Clockwise)

Preceding gene: 222526588

Following gene: 222526590

Centisome position: 79.4

GC content: 56.44

Gene sequence:

>606_bases
ATGGCGAAGAAAATCCTCTTTCTCGTTGGCGACTATGTCGAAGATTACGAGGTGATGGTACCATTTCAGGCCCTGGCCGC
ACTTGGTTATCAGGTTCACGCGGTTTGTCCGGGGAAGAAGGCGGGAGAGTTCGTGCGGACGGCGGTACACGATTTTGAAG
GCGATCAGACCTACAGCGAAAAACGTGGTCACAACTTTACTCTGAATTACTCCTTCGATGAAGTCAATGAGCACGAGTAC
GATGCGCTGGTCATTCCCGGTGGTCGTGCGCCAGAGTACATCCGGCTCAATCCACGGGTGCTTGAAATCACCCGCCACTT
CTTCACTGCGAATAAACCGGTAGCTGCAATCTGTCATGGTCTTCAGGTCCTGGCTGCCGCCGGGGTACTTAACGGTCGCC
GCTGCACCGCGTATGCGGCTGTCGGCCCAGACCTGCCGCTGATCGGGGCGGAGTATCACGAATTACCTGCCGATCAGGCA
ATTACTGACGGCAACCTGGTCACGGCACCGGCCTGGCCGGCTCATCCGGCGTGGCTGGCTCAGTTCCTCAAGGTACTCGG
TGCAAAGATCGAGATTGAACCACAGACCGAAGCTGTACCGGCATAA

Upstream 100 bases:

>100_bases
CACCGTTGACAGTTATCGGCCACTGTGTTACCGTAGAATCATCCCAGCCAGCAATCTACTGACAGTGAAGTCAACTGCAT
ACCGTCAGGAGGAGTACGCG

Downstream 100 bases:

>100_bases
CACCCACAGCGTGCGCTAACTGTTATGGTGAGTAGCACAGGCATCAGCCTGTGCTACTTCGTCAACGGGAACTCTACAGG
GGATGACTTCGTCTAACGAA

Product: intracellular protease, PfpI family

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MAKKILFLVGDYVEDYEVMVPFQALAALGYQVHAVCPGKKAGEFVRTAVHDFEGDQTYSEKRGHNFTLNYSFDEVNEHEY
DALVIPGGRAPEYIRLNPRVLEITRHFFTANKPVAAICHGLQVLAAAGVLNGRRCTAYAAVGPDLPLIGAEYHELPADQA
ITDGNLVTAPAWPAHPAWLAQFLKVLGAKIEIEPQTEAVPA

Sequences:

>Translated_201_residues
MAKKILFLVGDYVEDYEVMVPFQALAALGYQVHAVCPGKKAGEFVRTAVHDFEGDQTYSEKRGHNFTLNYSFDEVNEHEY
DALVIPGGRAPEYIRLNPRVLEITRHFFTANKPVAAICHGLQVLAAAGVLNGRRCTAYAAVGPDLPLIGAEYHELPADQA
ITDGNLVTAPAWPAHPAWLAQFLKVLGAKIEIEPQTEAVPA
>Mature_200_residues
AKKILFLVGDYVEDYEVMVPFQALAALGYQVHAVCPGKKAGEFVRTAVHDFEGDQTYSEKRGHNFTLNYSFDEVNEHEYD
ALVIPGGRAPEYIRLNPRVLEITRHFFTANKPVAAICHGLQVLAAAGVLNGRRCTAYAAVGPDLPLIGAEYHELPADQAI
TDGNLVTAPAWPAHPAWLAQFLKVLGAKIEIEPQTEAVPA

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=189, Percent_Identity=34.9206349206349, Blast_Score=107, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 21996; Mature: 21865

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKILFLVGDYVEDYEVMVPFQALAALGYQVHAVCPGKKAGEFVRTAVHDFEGDQTYSE
CCCEEEEEECCCCCCCEEEECHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHH
KRGHNFTLNYSFDEVNEHEYDALVIPGGRAPEYIRLNPRVLEITRHFFTANKPVAAICHG
HCCCEEEEEECHHCCCCCCCCEEEECCCCCCCEEEECCHHEEHHHHHHCCCCCHHHHHHH
LQVLAAAGVLNGRRCTAYAAVGPDLPLIGAEYHELPADQAITDGNLVTAPAWPAHPAWLA
HHHHHHHHCCCCCEEEEEEECCCCCCEECCHHHHCCCCCEECCCCEEECCCCCCCHHHHH
QFLKVLGAKIEIEPQTEAVPA
HHHHHHCCEEEECCCCCCCCC
>Mature Secondary Structure 
AKKILFLVGDYVEDYEVMVPFQALAALGYQVHAVCPGKKAGEFVRTAVHDFEGDQTYSE
CCEEEEEECCCCCCCEEEECHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHH
KRGHNFTLNYSFDEVNEHEYDALVIPGGRAPEYIRLNPRVLEITRHFFTANKPVAAICHG
HCCCEEEEEECHHCCCCCCCCEEEECCCCCCCEEEECCHHEEHHHHHHCCCCCHHHHHHH
LQVLAAAGVLNGRRCTAYAAVGPDLPLIGAEYHELPADQAITDGNLVTAPAWPAHPAWLA
HHHHHHHHCCCCCEEEEEEECCCCCCEECCHHHHCCCCCEECCCCEEECCCCCCCHHHHH
QFLKVLGAKIEIEPQTEAVPA
HHHHHHCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329 [H]