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The map label for this gene is metK

Identifier: 222526499

GI number: 222526499

Start: 4072435

End: 4073646

Strand: Reverse

Name: metK

Synonym: Chy400_3266

Alternate gene names: 222526499

Gene position: 4073646-4072435 (Counterclockwise)

Preceding gene: 222526500

Following gene: 222526498

Centisome position: 77.31

GC content: 58.42

Gene sequence:

>1212_bases
ATGGCAAACACATTTATGAACTCGCCTCGCTTCTACTTCACCAGTGAGAGTGTCAGCGAGGGCCATCCCGACAAAATGTG
TGATCAGATCAGCGACGCAATTCTCGATGCGTTCCTGAGTCACGACCCGAAGGCACGGGTGGCAGTGGAAACGGCGACAA
CCACCGGTCTGATCGTGGTGCTGGGTGAAGTAACTTACGAACGCGGCTACATTCCGATTGAAGAGATCGTCCGCCGCACG
GTGAAAGAGATTGGCTATACCAGCGCCGAATATGGCTTCGACGCCGACACCTGTGGCGTGATGGTCGCCATTCACGGTCA
ATCGCCCGATATTGCGATGGGTGTTGATAAAGCGCTTGAAGCCAAGATCGGCGCTATGGCCGATGACGTGGAAGCGGTTG
GGGCCGGCGATCAAGGGATGATGTTCGGCTTCGCCTGTGATGAGACGCCGGAACTGATGCCGGCCAGCATCGCCCTGGCC
CATCGCCTGATCCGTCGGCTGGAGCGGGTACGCAAAGATGGCACACTACCCTATCTGCGTCCCGACGCCAAATCGCAAGT
GACGGTTGAGTACAGCTTTGGCAAACCGGTGCGGGTTGATACTGTGCTGATCAGCAGCCAGCATGCACCGGACATCACTC
AGGATCAAATCCGCGCCGATTTGATCGAGCACGTCATCAAGACAGAAATTCCCGAAGCATGGCTGGATAGCCAGACCAAA
ATTTTCATCAATCCAACCGGTCGCTTCGTGATCGGTGGGCCAATGGGTGACAGCGGCCTCACCGGGCGCAAGATCATTGT
GGACACTTACGGTGGCGTTGCCCGCCATGGTGGTGGTGCCTTCAGCGGCAAAGACCCCTCAAAAGTTGATCGCAGTGCTG
CCTACGCCTGCCGCTGGGTTGCGAAGAACATTGTCGCCGCTGGACTGGCCCGTCGGGTCGAACTGCAAGTGAGCTACGCT
ATCGGCGTCGCCCGCCCGCTCTCGCTCAGCGTCGAAACCTTCGGTACCGCTGCCGTTCCCGATGAAGTCATCCTCAAAGC
GGTGAATGAGGTCTTTGATCTGCGGCCAGGTGCGATTATCCGCGATCTTGATCTGCGGCGTCCGATCTACCGCAAGACGG
CGGCTGGTGGACATTTCGGGCGGACTGACATCGATCTGCCATGGGAGCGCACGAATCGGGTGGAAGAGCTGCGCCGCGCT
GCCGGCTTGTAG

Upstream 100 bases:

>100_bases
GCAACCATCATTGCCTCGTTGCAGCCTTCCATTAACAAGTGACGTACAGCGCAGTAGGGCGCTGAAGAAATAAGCTCCAG
AACGACAACAGAGGTTACCT

Downstream 100 bases:

>100_bases
GCTTCTACCCCACCCGGCAGGATGTTTCGGGTAGTTCCTGAAGAGGATGATGTAAACCGGAACGTACCGGCACGCCGAAA
GGTGTGATGTATCTGTCAAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MANTFMNSPRFYFTSESVSEGHPDKMCDQISDAILDAFLSHDPKARVAVETATTTGLIVVLGEVTYERGYIPIEEIVRRT
VKEIGYTSAEYGFDADTCGVMVAIHGQSPDIAMGVDKALEAKIGAMADDVEAVGAGDQGMMFGFACDETPELMPASIALA
HRLIRRLERVRKDGTLPYLRPDAKSQVTVEYSFGKPVRVDTVLISSQHAPDITQDQIRADLIEHVIKTEIPEAWLDSQTK
IFINPTGRFVIGGPMGDSGLTGRKIIVDTYGGVARHGGGAFSGKDPSKVDRSAAYACRWVAKNIVAAGLARRVELQVSYA
IGVARPLSLSVETFGTAAVPDEVILKAVNEVFDLRPGAIIRDLDLRRPIYRKTAAGGHFGRTDIDLPWERTNRVEELRRA
AGL

Sequences:

>Translated_403_residues
MANTFMNSPRFYFTSESVSEGHPDKMCDQISDAILDAFLSHDPKARVAVETATTTGLIVVLGEVTYERGYIPIEEIVRRT
VKEIGYTSAEYGFDADTCGVMVAIHGQSPDIAMGVDKALEAKIGAMADDVEAVGAGDQGMMFGFACDETPELMPASIALA
HRLIRRLERVRKDGTLPYLRPDAKSQVTVEYSFGKPVRVDTVLISSQHAPDITQDQIRADLIEHVIKTEIPEAWLDSQTK
IFINPTGRFVIGGPMGDSGLTGRKIIVDTYGGVARHGGGAFSGKDPSKVDRSAAYACRWVAKNIVAAGLARRVELQVSYA
IGVARPLSLSVETFGTAAVPDEVILKAVNEVFDLRPGAIIRDLDLRRPIYRKTAAGGHFGRTDIDLPWERTNRVEELRRA
AGL
>Mature_402_residues
ANTFMNSPRFYFTSESVSEGHPDKMCDQISDAILDAFLSHDPKARVAVETATTTGLIVVLGEVTYERGYIPIEEIVRRTV
KEIGYTSAEYGFDADTCGVMVAIHGQSPDIAMGVDKALEAKIGAMADDVEAVGAGDQGMMFGFACDETPELMPASIALAH
RLIRRLERVRKDGTLPYLRPDAKSQVTVEYSFGKPVRVDTVLISSQHAPDITQDQIRADLIEHVIKTEIPEAWLDSQTKI
FINPTGRFVIGGPMGDSGLTGRKIIVDTYGGVARHGGGAFSGKDPSKVDRSAAYACRWVAKNIVAAGLARRVELQVSYAI
GVARPLSLSVETFGTAAVPDEVILKAVNEVFDLRPGAIIRDLDLRRPIYRKTAAGGHFGRTDIDLPWERTNRVEELRRAA
GL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=395, Percent_Identity=54.6835443037975, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI4557737, Length=383, Percent_Identity=56.1357702349869, Blast_Score=413, Evalue=1e-115,
Organism=Escherichia coli, GI1789311, Length=394, Percent_Identity=62.4365482233503, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17538494, Length=405, Percent_Identity=55.5555555555556, Blast_Score=436, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17538498, Length=394, Percent_Identity=56.3451776649746, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25145633, Length=394, Percent_Identity=56.0913705583756, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=55.0387596899225, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32565905, Length=405, Percent_Identity=51.6049382716049, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32565911, Length=343, Percent_Identity=53.935860058309, Blast_Score=357, Evalue=8e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=343, Percent_Identity=53.6443148688047, Blast_Score=353, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI32565903, Length=326, Percent_Identity=54.9079754601227, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI32565909, Length=88, Percent_Identity=54.5454545454545, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=43, Percent_Identity=74.4186046511628, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323209, Length=394, Percent_Identity=56.8527918781726, Blast_Score=425, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320710, Length=395, Percent_Identity=55.4430379746835, Blast_Score=419, Evalue=1e-118,
Organism=Drosophila melanogaster, GI45552159, Length=389, Percent_Identity=51.9280205655527, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=389, Percent_Identity=51.9280205655527, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=389, Percent_Identity=51.9280205655527, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=389, Percent_Identity=51.9280205655527, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=389, Percent_Identity=51.9280205655527, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=389, Percent_Identity=51.9280205655527, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=389, Percent_Identity=52.4421593830334, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580525, Length=389, Percent_Identity=52.4421593830334, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580541, Length=376, Percent_Identity=51.3297872340426, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580539, Length=128, Percent_Identity=53.125, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_CHLAA (A9WGQ3)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001636608.1
- ProteinModelPortal:   A9WGQ3
- SMR:   A9WGQ3
- GeneID:   5827494
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_3020
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43881; Mature: 43749

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTFMNSPRFYFTSESVSEGHPDKMCDQISDAILDAFLSHDPKARVAVETATTTGLIVV
CCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEE
LGEVTYERGYIPIEEIVRRTVKEIGYTSAEYGFDADTCGVMVAIHGQSPDIAMGVDKALE
EEEHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEECCHHHHHH
AKIGAMADDVEAVGAGDQGMMFGFACDETPELMPASIALAHRLIRRLERVRKDGTLPYLR
HHHCCHHHCHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
PDAKSQVTVEYSFGKPVRVDTVLISSQHAPDITQDQIRADLIEHVIKTEIPEAWLDSQTK
CCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCE
IFINPTGRFVIGGPMGDSGLTGRKIIVDTYGGVARHGGGAFSGKDPSKVDRSAAYACRWV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AKNIVAAGLARRVELQVSYAIGVARPLSLSVETFGTAAVPDEVILKAVNEVFDLRPGAII
HHHHHHHHHHHEEEEEEEEEECCCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHCCCCCEE
RDLDLRRPIYRKTAAGGHFGRTDIDLPWERTNRVEELRRAAGL
ECCHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANTFMNSPRFYFTSESVSEGHPDKMCDQISDAILDAFLSHDPKARVAVETATTTGLIVV
CCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEE
LGEVTYERGYIPIEEIVRRTVKEIGYTSAEYGFDADTCGVMVAIHGQSPDIAMGVDKALE
EEEHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEECCHHHHHH
AKIGAMADDVEAVGAGDQGMMFGFACDETPELMPASIALAHRLIRRLERVRKDGTLPYLR
HHHCCHHHCHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
PDAKSQVTVEYSFGKPVRVDTVLISSQHAPDITQDQIRADLIEHVIKTEIPEAWLDSQTK
CCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCE
IFINPTGRFVIGGPMGDSGLTGRKIIVDTYGGVARHGGGAFSGKDPSKVDRSAAYACRWV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHH
AKNIVAAGLARRVELQVSYAIGVARPLSLSVETFGTAAVPDEVILKAVNEVFDLRPGAII
HHHHHHHHHHHEEEEEEEEEECCCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHCCCCCEE
RDLDLRRPIYRKTAAGGHFGRTDIDLPWERTNRVEELRRAAGL
ECCHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA