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The map label for this gene is aam1 [H]

Identifier: 222526352

GI number: 222526352

Start: 3857396

End: 3859456

Strand: Direct

Name: aam1 [H]

Synonym: Chy400_3118

Alternate gene names: 222526352

Gene position: 3857396-3859456 (Clockwise)

Preceding gene: 222526351

Following gene: 222526354

Centisome position: 73.21

GC content: 56.14

Gene sequence:

>2061_bases
ATGAGTTACGAACTCTACGAACAGACTGACCAGATCATTTTACGCACCGCTCGTTATGAACTCGGCTGGTCAACGGATAG
TGGCGCTCTGATCGTCTTTCGTCGTGATAATGGCCCAAATATTCTCGGTTTTGGCCCTCCGGTTGCCGGGGTTGATGTTG
CTATCGGTTCGCCGACCAATTGGATTACCGCGCAGACGTTCGCGCGCTACATCTGGCATCGTTTGCAGATTCTGCCAGAT
GGTTACCTGCAGATGACAATTATTGTCGGTCTTGGCCCGTTCAAGGTGTTTGATCATTACCTGTTGTCTGACGAGGTGAT
CGAGCGCTGGATTGAGATCGAAAATGTCAGCGGTGATGAACTCCGCATGTACGGTGTACGGTTGATCATTCCTCATGTCC
AGATTGGCTCGCCAGATCGCTGTACCTTTGAGGCGCCGGGCAACAGTGTTCGGCCACGTGCACCGTATACCATCGCCTGC
ACCCAGGATCGTAACATTCTGCCCCGTCGTTTCTTTGCGCCCGGCTTACGTGGTGGCAGTGCCTTTGAGCCGGCACCAAC
CCAGGGTGCCGGGGTGATGGCTCTTGTCAATTCTGATCTCGCACTGACCTTCCTCTGCTGGTACGCCGGTGATGATGAGG
CGGCACTTCCATACGTGCAGGGCAGCCACACAGCAGTCTCTTTGGCCTATGAGATTGCTGTTGCCGGCTGGCTGCCCTCA
GATTGGCGGTTTGCGCTGGGACGGCAGTTTTTTACTCTTACCCCCGAACCCTGGCCGGCGGCATTGGCAATCTATCGGGC
ATTGACGCCATTCCCCCGTCCGCCAATGCCATGGCTGCGTGATGCAATCATCTATGTCGCCGATCTGCGACAGCACGGTG
GTGCTGATGGGTTGCGCCACCGTTTACCTGAACTGACAACGCTTGGGGTCGATACGCTATGTTTGTTACCGTGGCATACG
GTTGGTGATCGCCCGCATCTAATCGGCGATCTCGAACAGATCGATCCGGCGTGTGGTGATCACGAGACGATCCAACAGCT
TGTTGCAGCAGCTCACCAGCACGGCATGCGGGTCTTGGTAGATGTTGCCATGCAGGGTTGTGCTCCCGATTCTCGTTACC
TTAACGAACACCCGGAATGGTTTGTCCGTGATGAGTCAGGGGCATTTGTGATTGGGACACCGGCAGATGTACCGGCAGTG
GCGCGCCATCCCGGTGTTGCTCTTGCTACCGGTGGCTACCATTTCGACTGGCTACGCAGCGACTGGCGTCTGTACTGGCA
ACAATGGGTGATTGGCCAGGTTGATCACTTTGCCCTGGATGGGATACGGGCAGTGGCCCCATACAGCGCAGCGCCGGCCT
GGATACGGCGGGCATCGCTACGGGCGAGTACCGGTTCTGCTTTGATGGTGCAGACCCTACAAGAGATTGCGACAGCCCGG
CCAGAGCTTGCCATGATCTCTACTCTCTCCGGGCCACGGTCTGCCCAATTTGCCGGGGGATGGTTCGATTATCCCAGCCA
CCATATGCTCGTCCATCTGGCGATGCGCCGGATTACCCCTGCTGAGTGGTCGGCATATCAGAGAGATTATGCCGAACTCT
ATCCTGATACCTATCGGATTGGGTTTCTTGAATCTTTCGATACTGCCGATTGCAATCCGTTAGCCGATGGCTTACGCGGT
TCACGGCTTGGTCAGACGCTCTGGGCAGTGATGCTGTTTAGTGGTCTGACACCGGCGATCTGGAATGGTCAGGAGATCGC
AGATCGCACTGTCTTACCGCGCCTGTTGGCTTTATGGCGTAAAGAGCCGGCGCTTCGCTATGGGACAGTGGCTTACCAGG
ACCTTACGGCCGCTACTGGCGACATTATGATTATCTATCGTGAGTTTGAACAACGGCGCTTGATCGGCGTGATCAATCTG
GGCGCGTTACCGGGCCGATTCATTGCCCCCCAGCCTATTGGGCGTGATCTGCTCGAAATTTCACCGTTGAGTCGGGAACG
GCGCGGTATCGGTGAAGAATTGCGTCTGGCACCGTTCGGTATCTACTGCTTCGAGGGTTGA

Upstream 100 bases:

>100_bases
AGCGTGACCTTGCCTTATGAAGCGGCCATACTTCGTGTTATAATCTCTACAACCTGACAGGTAGTATCTACTACCTCTCC
TGACACTCGCCAATTGCTCA

Downstream 100 bases:

>100_bases
CATGGTAACAAGCACGTGACCCCCAGGTGTCGCGTCCATCTTTTTCCCGCTCCCGTCACGTAACACGCGAGGATTGACTA
TTACCCAAACCTGCCGAACG

Product: alpha amylase catalytic sub domain-containing protein

Products: D-glucose; cholesterol

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MSYELYEQTDQIILRTARYELGWSTDSGALIVFRRDNGPNILGFGPPVAGVDVAIGSPTNWITAQTFARYIWHRLQILPD
GYLQMTIIVGLGPFKVFDHYLLSDEVIERWIEIENVSGDELRMYGVRLIIPHVQIGSPDRCTFEAPGNSVRPRAPYTIAC
TQDRNILPRRFFAPGLRGGSAFEPAPTQGAGVMALVNSDLALTFLCWYAGDDEAALPYVQGSHTAVSLAYEIAVAGWLPS
DWRFALGRQFFTLTPEPWPAALAIYRALTPFPRPPMPWLRDAIIYVADLRQHGGADGLRHRLPELTTLGVDTLCLLPWHT
VGDRPHLIGDLEQIDPACGDHETIQQLVAAAHQHGMRVLVDVAMQGCAPDSRYLNEHPEWFVRDESGAFVIGTPADVPAV
ARHPGVALATGGYHFDWLRSDWRLYWQQWVIGQVDHFALDGIRAVAPYSAAPAWIRRASLRASTGSALMVQTLQEIATAR
PELAMISTLSGPRSAQFAGGWFDYPSHHMLVHLAMRRITPAEWSAYQRDYAELYPDTYRIGFLESFDTADCNPLADGLRG
SRLGQTLWAVMLFSGLTPAIWNGQEIADRTVLPRLLALWRKEPALRYGTVAYQDLTAATGDIMIIYREFEQRRLIGVINL
GALPGRFIAPQPIGRDLLEISPLSRERRGIGEELRLAPFGIYCFEG

Sequences:

>Translated_686_residues
MSYELYEQTDQIILRTARYELGWSTDSGALIVFRRDNGPNILGFGPPVAGVDVAIGSPTNWITAQTFARYIWHRLQILPD
GYLQMTIIVGLGPFKVFDHYLLSDEVIERWIEIENVSGDELRMYGVRLIIPHVQIGSPDRCTFEAPGNSVRPRAPYTIAC
TQDRNILPRRFFAPGLRGGSAFEPAPTQGAGVMALVNSDLALTFLCWYAGDDEAALPYVQGSHTAVSLAYEIAVAGWLPS
DWRFALGRQFFTLTPEPWPAALAIYRALTPFPRPPMPWLRDAIIYVADLRQHGGADGLRHRLPELTTLGVDTLCLLPWHT
VGDRPHLIGDLEQIDPACGDHETIQQLVAAAHQHGMRVLVDVAMQGCAPDSRYLNEHPEWFVRDESGAFVIGTPADVPAV
ARHPGVALATGGYHFDWLRSDWRLYWQQWVIGQVDHFALDGIRAVAPYSAAPAWIRRASLRASTGSALMVQTLQEIATAR
PELAMISTLSGPRSAQFAGGWFDYPSHHMLVHLAMRRITPAEWSAYQRDYAELYPDTYRIGFLESFDTADCNPLADGLRG
SRLGQTLWAVMLFSGLTPAIWNGQEIADRTVLPRLLALWRKEPALRYGTVAYQDLTAATGDIMIIYREFEQRRLIGVINL
GALPGRFIAPQPIGRDLLEISPLSRERRGIGEELRLAPFGIYCFEG
>Mature_685_residues
SYELYEQTDQIILRTARYELGWSTDSGALIVFRRDNGPNILGFGPPVAGVDVAIGSPTNWITAQTFARYIWHRLQILPDG
YLQMTIIVGLGPFKVFDHYLLSDEVIERWIEIENVSGDELRMYGVRLIIPHVQIGSPDRCTFEAPGNSVRPRAPYTIACT
QDRNILPRRFFAPGLRGGSAFEPAPTQGAGVMALVNSDLALTFLCWYAGDDEAALPYVQGSHTAVSLAYEIAVAGWLPSD
WRFALGRQFFTLTPEPWPAALAIYRALTPFPRPPMPWLRDAIIYVADLRQHGGADGLRHRLPELTTLGVDTLCLLPWHTV
GDRPHLIGDLEQIDPACGDHETIQQLVAAAHQHGMRVLVDVAMQGCAPDSRYLNEHPEWFVRDESGAFVIGTPADVPAVA
RHPGVALATGGYHFDWLRSDWRLYWQQWVIGQVDHFALDGIRAVAPYSAAPAWIRRASLRASTGSALMVQTLQEIATARP
ELAMISTLSGPRSAQFAGGWFDYPSHHMLVHLAMRRITPAEWSAYQRDYAELYPDTYRIGFLESFDTADCNPLADGLRGS
RLGQTLWAVMLFSGLTPAIWNGQEIADRTVLPRLLALWRKEPALRYGTVAYQDLTAATGDIMIIYREFEQRRLIGVINLG
ALPGRFIAPQPIGRDLLEISPLSRERRGIGEELRLAPFGIYCFEG

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. GlgE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021828
- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF11896 DUF3416 [H]

EC number: 3.2.1.1

Molecular weight: Translated: 76538; Mature: 76407

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYELYEQTDQIILRTARYELGWSTDSGALIVFRRDNGPNILGFGPPVAGVDVAIGSPTN
CCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECCCCEEEECCCCCCCEEEEECCCCC
WITAQTFARYIWHRLQILPDGYLQMTIIVGLGPFKVFDHYLLSDEVIERWIEIENVSGDE
CEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LRMYGVRLIIPHVQIGSPDRCTFEAPGNSVRPRAPYTIACTQDRNILPRRFFAPGLRGGS
EEEEEEEEEEEEEECCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCHHHCCCCCCCCC
AFEPAPTQGAGVMALVNSDLALTFLCWYAGDDEAALPYVQGSHTAVSLAYEIAVAGWLPS
CCCCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCEECCCCCEEEEEEEEEEECCCCC
DWRFALGRQFFTLTPEPWPAALAIYRALTPFPRPPMPWLRDAIIYVADLRQHGGADGLRH
CHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
RLPELTTLGVDTLCLLPWHTVGDRPHLIGDLEQIDPACGDHETIQQLVAAAHQHGMRVLV
HCCCHHHCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCHHHH
DVAMQGCAPDSRYLNEHPEWFVRDESGAFVIGTPADVPAVARHPGVALATGGYHFDWLRS
HHHHHCCCCCHHHHCCCCCEEEEECCCCEEEECCCCCCHHHCCCCEEEEECCEEEHHHHH
DWRLYWQQWVIGQVDHFALDGIRAVAPYSAAPAWIRRASLRASTGSALMVQTLQEIATAR
HHHHHHHHHHHCCHHHHHHCCHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
PELAMISTLSGPRSAQFAGGWFDYPSHHMLVHLAMRRITPAEWSAYQRDYAELYPDTYRI
CCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEE
GFLESFDTADCNPLADGLRGSRLGQTLWAVMLFSGLTPAIWNGQEIADRTVLPRLLALWR
EEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
KEPALRYGTVAYQDLTAATGDIMIIYREFEQRRLIGVINLGALPGRFIAPQPIGRDLLEI
CCCCCEECCEEHHHHHCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCHHHHC
SPLSRERRGIGEELRLAPFGIYCFEG
CCCCHHHHCCCCCEEECEEEEEEECC
>Mature Secondary Structure 
SYELYEQTDQIILRTARYELGWSTDSGALIVFRRDNGPNILGFGPPVAGVDVAIGSPTN
CCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECCCCEEEECCCCCCCEEEEECCCCC
WITAQTFARYIWHRLQILPDGYLQMTIIVGLGPFKVFDHYLLSDEVIERWIEIENVSGDE
CEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LRMYGVRLIIPHVQIGSPDRCTFEAPGNSVRPRAPYTIACTQDRNILPRRFFAPGLRGGS
EEEEEEEEEEEEEECCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCHHHCCCCCCCCC
AFEPAPTQGAGVMALVNSDLALTFLCWYAGDDEAALPYVQGSHTAVSLAYEIAVAGWLPS
CCCCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCEECCCCCEEEEEEEEEEECCCCC
DWRFALGRQFFTLTPEPWPAALAIYRALTPFPRPPMPWLRDAIIYVADLRQHGGADGLRH
CHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
RLPELTTLGVDTLCLLPWHTVGDRPHLIGDLEQIDPACGDHETIQQLVAAAHQHGMRVLV
HCCCHHHCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCHHHH
DVAMQGCAPDSRYLNEHPEWFVRDESGAFVIGTPADVPAVARHPGVALATGGYHFDWLRS
HHHHHCCCCCHHHHCCCCCEEEEECCCCEEEECCCCCCHHHCCCCEEEEECCEEEHHHHH
DWRLYWQQWVIGQVDHFALDGIRAVAPYSAAPAWIRRASLRASTGSALMVQTLQEIATAR
HHHHHHHHHHHCCHHHHHHCCHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
PELAMISTLSGPRSAQFAGGWFDYPSHHMLVHLAMRRITPAEWSAYQRDYAELYPDTYRI
CCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEE
GFLESFDTADCNPLADGLRGSRLGQTLWAVMLFSGLTPAIWNGQEIADRTVLPRLLALWR
EEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
KEPALRYGTVAYQDLTAATGDIMIIYREFEQRRLIGVINLGALPGRFIAPQPIGRDLLEI
CCCCCEECCEEHHHHHCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCHHHHC
SPLSRERRGIGEELRLAPFGIYCFEG
CCCCHHHHCCCCCEEECEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; cholesteryl-beta-D-glucoside

Specific reaction: H2O + cholesteryl-beta-D-glucoside = D-glucose + cholesterol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10970853 [H]