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The map label for this gene is tgt [H]

Identifier: 222526315

GI number: 222526315

Start: 3799635

End: 3800747

Strand: Direct

Name: tgt [H]

Synonym: Chy400_3081

Alternate gene names: 222526315

Gene position: 3799635-3800747 (Clockwise)

Preceding gene: 222526313

Following gene: 222526316

Centisome position: 72.11

GC content: 56.24

Gene sequence:

>1113_bases
ATGACGGACTTCTTGACTCTTCAGCATGGCCAGTTATCCTTACCGACATTTTTGCCCGATGCAACCTTTGGCACGGTGCG
CGCAGTTGATAGCCGTGATCTCAGCGAAGCCGGTATCAGAGCGTTGGTCATGAATGTCTTTCATCTCATGCAACGTCCCG
GTAGTTCGACGATCACCGCCTTAGGAGGGTTGCACCAGATGACAGCCTGGTCGGGGCCAATTGTGACCGATAGCGGTGGT
TTTCAAGCCTATTCGCTGATCCGAAATAATTCCAAACAGGGTCGGATTACCGATCAGGGGTTAACCTTTCAACCAGAGGG
GGCTGAACGGCGGTTTCAACTGACGCCGGAGAAGAGCATTCAACTGCAATTGAGCTATGGCGCTGATATCGTCATTTGCC
TTGACGATTGCACACACGTTGACGACCCACCTGCCGAACAGCGCCGTTCGGTTGAACGGACGATTCGCTGGGCCCGTCGC
TGTCGGCAGGAATTTGATCGTCAGGTAGCACAGCGCCGACGCCAGGGTCCGCCACCATTGCTCTTTGCTGTGGTACAGGG
TGGGGGCGATCTGCATTTGCGCGCCGAGTGTGCAGCCGCACTTCTCGACATTGGGTTCGACGGATTCGGGTTTGGGGGAT
GGCCGCTCGATGCCCAGGGCCAACTTCTCGATGAGGTGCTGGCGTTCACCCGTGATCAAATTCCCGCTCGCTTTCCTATG
CACGCGCTGGGGGTTGGACACCCGGCCAGTGTAGTGGCCTGTGCCCGGATGGGTTACGACATCTTCGATAGTGCATTACC
GACCCGCGATGCTCGTCAGGGGCGTCTGCTGGCGTTTACGGCTGATCCGCAGCGACCGGGCTTCCAATTGCGCGGTGATT
GGTTTACCTATGTTTATCTGGCCGACCGCAAGCATGTGAAAGCTGATACACCAATCTCGCCGGGATGCCAGTGCTTTACC
TGTAGCCGGTACAGTCTGGGGTATTTGCATCATCTGCACAAACTGGGTGAGACGCTGGTCTTGCGATTGGCAACCATCCA
CAATCTCCACTTCATGGCGCAGTTGATGGAGCTGATCCGGCACGAACGGAGAGGATCGCAAGCTAATGACTAA

Upstream 100 bases:

>100_bases
GACACAAACAGGAATCAAATATTGCGACTTCTGCGATAGTATAGCGCACGATTTACCAAATTTCAACCCTCGAATGTCTA
TATAATTGTTGGGATGGTAT

Downstream 100 bases:

>100_bases
TTGTGAGACGCAACTGGCTGAATTGGTAGCAGCAGTTCAAACCAGCGCACGTTACCGAACGATTGATCGGCAACTGGTCG
AGCGTGTCGGTCGGGAAGCA

Product: tRNA-guanine transglycosylase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTDFLTLQHGQLSLPTFLPDATFGTVRAVDSRDLSEAGIRALVMNVFHLMQRPGSSTITALGGLHQMTAWSGPIVTDSGG
FQAYSLIRNNSKQGRITDQGLTFQPEGAERRFQLTPEKSIQLQLSYGADIVICLDDCTHVDDPPAEQRRSVERTIRWARR
CRQEFDRQVAQRRRQGPPPLLFAVVQGGGDLHLRAECAAALLDIGFDGFGFGGWPLDAQGQLLDEVLAFTRDQIPARFPM
HALGVGHPASVVACARMGYDIFDSALPTRDARQGRLLAFTADPQRPGFQLRGDWFTYVYLADRKHVKADTPISPGCQCFT
CSRYSLGYLHHLHKLGETLVLRLATIHNLHFMAQLMELIRHERRGSQAND

Sequences:

>Translated_370_residues
MTDFLTLQHGQLSLPTFLPDATFGTVRAVDSRDLSEAGIRALVMNVFHLMQRPGSSTITALGGLHQMTAWSGPIVTDSGG
FQAYSLIRNNSKQGRITDQGLTFQPEGAERRFQLTPEKSIQLQLSYGADIVICLDDCTHVDDPPAEQRRSVERTIRWARR
CRQEFDRQVAQRRRQGPPPLLFAVVQGGGDLHLRAECAAALLDIGFDGFGFGGWPLDAQGQLLDEVLAFTRDQIPARFPM
HALGVGHPASVVACARMGYDIFDSALPTRDARQGRLLAFTADPQRPGFQLRGDWFTYVYLADRKHVKADTPISPGCQCFT
CSRYSLGYLHHLHKLGETLVLRLATIHNLHFMAQLMELIRHERRGSQAND
>Mature_369_residues
TDFLTLQHGQLSLPTFLPDATFGTVRAVDSRDLSEAGIRALVMNVFHLMQRPGSSTITALGGLHQMTAWSGPIVTDSGGF
QAYSLIRNNSKQGRITDQGLTFQPEGAERRFQLTPEKSIQLQLSYGADIVICLDDCTHVDDPPAEQRRSVERTIRWARRC
RQEFDRQVAQRRRQGPPPLLFAVVQGGGDLHLRAECAAALLDIGFDGFGFGGWPLDAQGQLLDEVLAFTRDQIPARFPMH
ALGVGHPASVVACARMGYDIFDSALPTRDARQGRLLAFTADPQRPGFQLRGDWFTYVYLADRKHVKADTPISPGCQCFTC
SRYSLGYLHHLHKLGETLVLRLATIHNLHFMAQLMELIRHERRGSQAND

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=358, Percent_Identity=33.2402234636872, Blast_Score=188, Evalue=6e-48,
Organism=Escherichia coli, GI1786607, Length=358, Percent_Identity=36.5921787709497, Blast_Score=228, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17544680, Length=366, Percent_Identity=33.0601092896175, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI19920510, Length=360, Percent_Identity=32.5, Blast_Score=192, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41236; Mature: 41104

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFLTLQHGQLSLPTFLPDATFGTVRAVDSRDLSEAGIRALVMNVFHLMQRPGSSTITA
CCCEEEECCCCEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
LGGLHQMTAWSGPIVTDSGGFQAYSLIRNNSKQGRITDQGLTFQPEGAERRFQLTPEKSI
HCCHHHHHCCCCCEEECCCCCEEHHHHHCCCCCCCEECCCCEECCCCCCCEEEECCCCCE
QLQLSYGADIVICLDDCTHVDDPPAEQRRSVERTIRWARRCRQEFDRQVAQRRRQGPPPL
EEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LFAVVQGGGDLHLRAECAAALLDIGFDGFGFGGWPLDAQGQLLDEVLAFTRDQIPARFPM
EEEEEECCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
HALGVGHPASVVACARMGYDIFDSALPTRDARQGRLLAFTADPQRPGFQLRGDWFTYVYL
HHHCCCCHHHHHHHHHHCHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEECCCEEEEEEE
ADRKHVKADTPISPGCQCFTCSRYSLGYLHHLHKLGETLVLRLATIHNLHFMAQLMELIR
ECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HERRGSQAND
HHHCCCCCCC
>Mature Secondary Structure 
TDFLTLQHGQLSLPTFLPDATFGTVRAVDSRDLSEAGIRALVMNVFHLMQRPGSSTITA
CCEEEECCCCEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
LGGLHQMTAWSGPIVTDSGGFQAYSLIRNNSKQGRITDQGLTFQPEGAERRFQLTPEKSI
HCCHHHHHCCCCCEEECCCCCEEHHHHHCCCCCCCEECCCCEECCCCCCCEEEECCCCCE
QLQLSYGADIVICLDDCTHVDDPPAEQRRSVERTIRWARRCRQEFDRQVAQRRRQGPPPL
EEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LFAVVQGGGDLHLRAECAAALLDIGFDGFGFGGWPLDAQGQLLDEVLAFTRDQIPARFPM
EEEEEECCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
HALGVGHPASVVACARMGYDIFDSALPTRDARQGRLLAFTADPQRPGFQLRGDWFTYVYL
HHHCCCCHHHHHHHHHHCHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEECCCEEEEEEE
ADRKHVKADTPISPGCQCFTCSRYSLGYLHHLHKLGETLVLRLATIHNLHFMAQLMELIR
ECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HERRGSQAND
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA