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The map label for this gene is appA [H]

Identifier: 222526247

GI number: 222526247

Start: 3718681

End: 3720411

Strand: Reverse

Name: appA [H]

Synonym: Chy400_3011

Alternate gene names: 222526247

Gene position: 3720411-3718681 (Counterclockwise)

Preceding gene: 222526248

Following gene: 222526246

Centisome position: 70.61

GC content: 50.72

Gene sequence:

>1731_bases
ATGAAACCCTCGCACTCATTGTTAATCATCGCCGTACTTTTGGTCCTGACAGCCTGTAGCAGTTCACCCTCATCTTCGCC
ATCCGGTTCATCGCAAACTATGCCGACCACGGCCGTCACTCAACCCACGGCCGACCAGCCTGCTGTTGACCCAAATGCCC
CCAAACAGGGCGGAACGTTAATTTTTGGTACCCCGCAAGAACCGGCTATTCTTAACACATTACTGACAACCTCGTCTATC
GAAGATGCTGTCACCTCACTGTTTGTTGAAGGACTGGTTCAGGTCAATGATAAAGGTGAATATGTACCAGTTCTGGCTGA
AGAACTGCCGACCGTCTCAGAGGATGGGCTGGTTGTAACGTGGAAGCTACGCAAGGGTGTAAGGTTCTCAAACGGGCTAG
AATTCACATGCGAAGATGTGAAATTTACGATGGAAGGTGCTCTGTCTGACCTCTCCCAGGTTAGCACATCCGGGTATAGC
TCTATCGAAACACTGGAATGCCCTGATCCATACACTGTCGTTGCTACCTTCTCGGAAGTATACGCACCTTATCTGCGTCT
ATTTGCTTACATCGTGCCCCATGATGCCGGCCCTCTCGATCAGATGGAAACCTGGGCATACAATCAGAAACCGTGGGGAA
CGGGACCTTTCGTCCTTGAAGAATGGGTTGCTGGCGATCATCTCACATTTGTGCGAAATCCATACTACCGTGAAGAAGGA
AAACCATACCTGGATAAAGTCATTATTCGCGTCTTACCGTCACGCGAAGTGGGCATTCAATTGTTAGGAACCGGTGAGAT
TCATGCCTTGTGGGACATTACCGAAGCTGATCTGGCCACTATCGAACAGTCTGCTGGTCAGAATGTAACCTATAAAGCGA
CGGTAACCGGCGAGAACGAGCTGCTTGTCCTGAACTTTGGCAATAATGATGGCAGTGCTCCGGCCGATCCTGCCACGAAT
CCGCATCCCATTCTCTACGACCTGCGCGTGCGGCAGGCGATTCAATACGCCATCAATAAACAAATGATTGCTGACACCCT
CTTGTATGGAAAGGTCAAACCGGGTAGTACCGTCCTTCCTGCCGGTCCGTTTGCCTGCCCTCAACCAGCCAGCGAATACA
ATCCTGAGCGGGCAAAGGCATTGCTCGAAGAAGCGGGCTGGCAAGTTGGCGCCGATGGCATTCGGGAAAAAGACGGTATG
CGCCTGAGCCTGAAAATAAACTCAACGAGCGGCAACTTGCTGCGCGAGCAAACCGAACAGGTACTTGCCGAGATGTTGAA
ACAAGTGGGGATTGAACTCACGATTGAAAATATGCCATCAGACGTCTTCTTCGCCGGTGGGGAAAGTGACGGCATTCGCG
ACACAGGCAAGTTCGATATTATTCTCTACACAACCGGGCCAGGGCTTGATCCAGACAGCCATCTGTTCAGCAACTACAGC
AGTGCTCGTATTCCGACTGCTGAAAATGATTGGGCAGGTGCAAATTTCAGCCGCTATATTAACCCCGATGTAGATGCATG
GATTGATGAGGCTGCTATCGTTATGGACCAGGATGTCCGCAAAGAACTTTACTGCAAGGTAGCAACCCAGATTAACCAGG
ACTTGCCACGCATCTTTCTGTACGAGCGGCTATCAATCACTGCACACCGACAGGAATTTCACAACTTGACCATTTCACCC
AGTTTCGTCGATTTTGCATGGGCCGCACAGGATTGGTGGCTCGATCAGTGA

Upstream 100 bases:

>100_bases
CACCTACGATTGTGTGAATGGTAACGCGCATATTACCGTTGTGCCCTCGCTTAGCGATGAGTAGCCAGAGACCGCCTGAT
TTATCTACAAGGAGCACTGC

Downstream 100 bases:

>100_bases
TTATATTGCTTACCAGGGTGCTATGACTACCTATCTGCTACGACGACTGCTACAGGCAGTACTGACACTCTTTGTAGTCA
GCCTGATCCTCTTTGGGCTG

Product: family 5 extracellular solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MKPSHSLLIIAVLLVLTACSSSPSSSPSGSSQTMPTTAVTQPTADQPAVDPNAPKQGGTLIFGTPQEPAILNTLLTTSSI
EDAVTSLFVEGLVQVNDKGEYVPVLAEELPTVSEDGLVVTWKLRKGVRFSNGLEFTCEDVKFTMEGALSDLSQVSTSGYS
SIETLECPDPYTVVATFSEVYAPYLRLFAYIVPHDAGPLDQMETWAYNQKPWGTGPFVLEEWVAGDHLTFVRNPYYREEG
KPYLDKVIIRVLPSREVGIQLLGTGEIHALWDITEADLATIEQSAGQNVTYKATVTGENELLVLNFGNNDGSAPADPATN
PHPILYDLRVRQAIQYAINKQMIADTLLYGKVKPGSTVLPAGPFACPQPASEYNPERAKALLEEAGWQVGADGIREKDGM
RLSLKINSTSGNLLREQTEQVLAEMLKQVGIELTIENMPSDVFFAGGESDGIRDTGKFDIILYTTGPGLDPDSHLFSNYS
SARIPTAENDWAGANFSRYINPDVDAWIDEAAIVMDQDVRKELYCKVATQINQDLPRIFLYERLSITAHRQEFHNLTISP
SFVDFAWAAQDWWLDQ

Sequences:

>Translated_576_residues
MKPSHSLLIIAVLLVLTACSSSPSSSPSGSSQTMPTTAVTQPTADQPAVDPNAPKQGGTLIFGTPQEPAILNTLLTTSSI
EDAVTSLFVEGLVQVNDKGEYVPVLAEELPTVSEDGLVVTWKLRKGVRFSNGLEFTCEDVKFTMEGALSDLSQVSTSGYS
SIETLECPDPYTVVATFSEVYAPYLRLFAYIVPHDAGPLDQMETWAYNQKPWGTGPFVLEEWVAGDHLTFVRNPYYREEG
KPYLDKVIIRVLPSREVGIQLLGTGEIHALWDITEADLATIEQSAGQNVTYKATVTGENELLVLNFGNNDGSAPADPATN
PHPILYDLRVRQAIQYAINKQMIADTLLYGKVKPGSTVLPAGPFACPQPASEYNPERAKALLEEAGWQVGADGIREKDGM
RLSLKINSTSGNLLREQTEQVLAEMLKQVGIELTIENMPSDVFFAGGESDGIRDTGKFDIILYTTGPGLDPDSHLFSNYS
SARIPTAENDWAGANFSRYINPDVDAWIDEAAIVMDQDVRKELYCKVATQINQDLPRIFLYERLSITAHRQEFHNLTISP
SFVDFAWAAQDWWLDQ
>Mature_576_residues
MKPSHSLLIIAVLLVLTACSSSPSSSPSGSSQTMPTTAVTQPTADQPAVDPNAPKQGGTLIFGTPQEPAILNTLLTTSSI
EDAVTSLFVEGLVQVNDKGEYVPVLAEELPTVSEDGLVVTWKLRKGVRFSNGLEFTCEDVKFTMEGALSDLSQVSTSGYS
SIETLECPDPYTVVATFSEVYAPYLRLFAYIVPHDAGPLDQMETWAYNQKPWGTGPFVLEEWVAGDHLTFVRNPYYREEG
KPYLDKVIIRVLPSREVGIQLLGTGEIHALWDITEADLATIEQSAGQNVTYKATVTGENELLVLNFGNNDGSAPADPATN
PHPILYDLRVRQAIQYAINKQMIADTLLYGKVKPGSTVLPAGPFACPQPASEYNPERAKALLEEAGWQVGADGIREKDGM
RLSLKINSTSGNLLREQTEQVLAEMLKQVGIELTIENMPSDVFFAGGESDGIRDTGKFDIILYTTGPGLDPDSHLFSNYS
SARIPTAENDWAGANFSRYINPDVDAWIDEAAIVMDQDVRKELYCKVATQINQDLPRIFLYERLSITAHRQEFHNLTISP
SFVDFAWAAQDWWLDQ

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=466, Percent_Identity=28.755364806867, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787052, Length=499, Percent_Identity=24.4488977955912, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1789966, Length=544, Percent_Identity=24.8161764705882, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787762, Length=516, Percent_Identity=24.031007751938, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1787551, Length=531, Percent_Identity=22.0338983050847, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63282; Mature: 63282

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSHSLLIIAVLLVLTACSSSPSSSPSGSSQTMPTTAVTQPTADQPAVDPNAPKQGGTL
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
IFGTPQEPAILNTLLTTSSIEDAVTSLFVEGLVQVNDKGEYVPVLAEELPTVSEDGLVVT
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEHHHHHCCCCCCCCEEEE
WKLRKGVRFSNGLEFTCEDVKFTMEGALSDLSQVSTSGYSSIETLECPDPYTVVATFSEV
EEECCCCEECCCCEEEECCCEEEHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHH
YAPYLRLFAYIVPHDAGPLDQMETWAYNQKPWGTGPFVLEEWVAGDHLTFVRNPYYREEG
HHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCC
KPYLDKVIIRVLPSREVGIQLLGTGEIHALWDITEADLATIEQSAGQNVTYKATVTGENE
CCHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHCCCCEEEEEEEECCCE
LLVLNFGNNDGSAPADPATNPHPILYDLRVRQAIQYAINKQMIADTLLYGKVKPGSTVLP
EEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECC
AGPFACPQPASEYNPERAKALLEEAGWQVGADGIREKDGMRLSLKINSTSGNLLREQTEQ
CCCCCCCCCCHHCCHHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
VLAEMLKQVGIELTIENMPSDVFFAGGESDGIRDTGKFDIILYTTGPGLDPDSHLFSNYS
HHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHCCCC
SARIPTAENDWAGANFSRYINPDVDAWIDEAAIVMDQDVRKELYCKVATQINQDLPRIFL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHEEECHHHHHHHHHHHHHHHHHCCCHHHH
YERLSITAHRQEFHNLTISPSFVDFAWAAQDWWLDQ
HHHHHHHHHHHHHCCEECCCCHHHHEECCCHHCCCC
>Mature Secondary Structure
MKPSHSLLIIAVLLVLTACSSSPSSSPSGSSQTMPTTAVTQPTADQPAVDPNAPKQGGTL
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
IFGTPQEPAILNTLLTTSSIEDAVTSLFVEGLVQVNDKGEYVPVLAEELPTVSEDGLVVT
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEHHHHHCCCCCCCCEEEE
WKLRKGVRFSNGLEFTCEDVKFTMEGALSDLSQVSTSGYSSIETLECPDPYTVVATFSEV
EEECCCCEECCCCEEEECCCEEEHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHH
YAPYLRLFAYIVPHDAGPLDQMETWAYNQKPWGTGPFVLEEWVAGDHLTFVRNPYYREEG
HHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCC
KPYLDKVIIRVLPSREVGIQLLGTGEIHALWDITEADLATIEQSAGQNVTYKATVTGENE
CCHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHCCCCEEEEEEEECCCE
LLVLNFGNNDGSAPADPATNPHPILYDLRVRQAIQYAINKQMIADTLLYGKVKPGSTVLP
EEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECC
AGPFACPQPASEYNPERAKALLEEAGWQVGADGIREKDGMRLSLKINSTSGNLLREQTEQ
CCCCCCCCCCHHCCHHHHHHHHHHCCCEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
VLAEMLKQVGIELTIENMPSDVFFAGGESDGIRDTGKFDIILYTTGPGLDPDSHLFSNYS
HHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHCCCC
SARIPTAENDWAGANFSRYINPDVDAWIDEAAIVMDQDVRKELYCKVATQINQDLPRIFL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHEEECHHHHHHHHHHHHHHHHHCCCHHHH
YERLSITAHRQEFHNLTISPSFVDFAWAAQDWWLDQ
HHHHHHHHHHHHHCCEECCCCHHHHEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]