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The map label for this gene is appB [H]

Identifier: 222526246

GI number: 222526246

Start: 3717687

End: 3718658

Strand: Reverse

Name: appB [H]

Synonym: Chy400_3010

Alternate gene names: 222526246

Gene position: 3718658-3717687 (Counterclockwise)

Preceding gene: 222526247

Following gene: 222526245

Centisome position: 70.58

GC content: 49.79

Gene sequence:

>972_bases
ATGACTACCTATCTGCTACGACGACTGCTACAGGCAGTACTGACACTCTTTGTAGTCAGCCTGATCCTCTTTGGGCTGAT
TTCAGTAACGCCGGGTGGTTTCATGACCGTGTATGCTGAAAAATCAGACATGACGGCAGCAGACCTGGCGCGCATCCGGG
CAAATTTAGGATTAGACGATCCGGTACCAATACGTTACGTGAAATGGCTCGGAAATCTGCTCAAAGGGGATTGGGGTCGC
TCCTTAACGTCAAAGCGCCCGGTATTGGAAGAGATCGGCAGTCGATTACCCAACACCCTATTGCTGATGAGTTTGATGCT
GATATGCACACTACTGGTCGCTATTCCGCTGGGAATCCTATCGGCAATCAGACAATACTCATGGCTGGACAACGTACTCA
CCACGATTGCGTTTGCCGGGCAATCGCTGCCGGTCTTCTGGTTTGGGCTACTTCTGATTATTGTCTTCTCGGTGCTTTTA
CGAGGTCCAGACGGAAAGCCTCTTCTACCGGGATCAGGTATGTACACTCTCGGTGAGCCATTCTCTCTGTGGGATCGTAT
CCGTCATTTAATTTTACCGGTGACCATGCTCACCTTTGTCTCCGCTGCCGAATATATGCGCTATATGCGTTCGGCGATGC
TGGACGTCATTCACGAGGACTACATTCGCACGGCCCGTGCGAAAGGTATTCAAGAATGGGTCGTTATTTATAAACATGCC
TTACGCAATGCGATTATTCCGCTGGTCACCCTGCTCAGCCTCGATCTACCATCACTATTTGCCGGAGCACTATTTACCGA
GACGATCTTTGCGTGGCCGGGTATCGGGCGGCTCTACTTCACAGCCGCCCTCAAAAATGACTACCCCCTGGTTATGGCTA
TTCTTACAATCTATTCTGCTCTGATAATCCTTTCCAACTTATTGGTTGATCTGATCTATGCCATGCTTGATCCACGCATT
CGACTGTCATGA

Upstream 100 bases:

>100_bases
GAATTTCACAACTTGACCATTTCACCCAGTTTCGTCGATTTTGCATGGGCCGCACAGGATTGGTGGCTCGATCAGTGATT
ATATTGCTTACCAGGGTGCT

Downstream 100 bases:

>100_bases
AAGACTCACAATCACCCTGGTTCTTGTTCGTACAACGCTTTCGCAAGCATACTATGGCACTTGTCGGTATGGTCATTCTG
GTAAGTATTACCATACTGGT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTTYLLRRLLQAVLTLFVVSLILFGLISVTPGGFMTVYAEKSDMTAADLARIRANLGLDDPVPIRYVKWLGNLLKGDWGR
SLTSKRPVLEEIGSRLPNTLLLMSLMLICTLLVAIPLGILSAIRQYSWLDNVLTTIAFAGQSLPVFWFGLLLIIVFSVLL
RGPDGKPLLPGSGMYTLGEPFSLWDRIRHLILPVTMLTFVSAAEYMRYMRSAMLDVIHEDYIRTARAKGIQEWVVIYKHA
LRNAIIPLVTLLSLDLPSLFAGALFTETIFAWPGIGRLYFTAALKNDYPLVMAILTIYSALIILSNLLVDLIYAMLDPRI
RLS

Sequences:

>Translated_323_residues
MTTYLLRRLLQAVLTLFVVSLILFGLISVTPGGFMTVYAEKSDMTAADLARIRANLGLDDPVPIRYVKWLGNLLKGDWGR
SLTSKRPVLEEIGSRLPNTLLLMSLMLICTLLVAIPLGILSAIRQYSWLDNVLTTIAFAGQSLPVFWFGLLLIIVFSVLL
RGPDGKPLLPGSGMYTLGEPFSLWDRIRHLILPVTMLTFVSAAEYMRYMRSAMLDVIHEDYIRTARAKGIQEWVVIYKHA
LRNAIIPLVTLLSLDLPSLFAGALFTETIFAWPGIGRLYFTAALKNDYPLVMAILTIYSALIILSNLLVDLIYAMLDPRI
RLS
>Mature_322_residues
TTYLLRRLLQAVLTLFVVSLILFGLISVTPGGFMTVYAEKSDMTAADLARIRANLGLDDPVPIRYVKWLGNLLKGDWGRS
LTSKRPVLEEIGSRLPNTLLLMSLMLICTLLVAIPLGILSAIRQYSWLDNVLTTIAFAGQSLPVFWFGLLLIIVFSVLLR
GPDGKPLLPGSGMYTLGEPFSLWDRIRHLILPVTMLTFVSAAEYMRYMRSAMLDVIHEDYIRTARAKGIQEWVVIYKHAL
RNAIIPLVTLLSLDLPSLFAGALFTETIFAWPGIGRLYFTAALKNDYPLVMAILTIYSALIILSNLLVDLIYAMLDPRIR
LS

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=339, Percent_Identity=37.4631268436578, Blast_Score=217, Evalue=6e-58,
Organism=Escherichia coli, GI1787053, Length=322, Percent_Identity=38.1987577639752, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1789888, Length=323, Percent_Identity=35.6037151702786, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1787761, Length=335, Percent_Identity=38.5074626865672, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1787497, Length=324, Percent_Identity=30.8641975308642, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787550, Length=274, Percent_Identity=31.3868613138686, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788504, Length=366, Percent_Identity=27.8688524590164, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36149; Mature: 36018

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYLLRRLLQAVLTLFVVSLILFGLISVTPGGFMTVYAEKSDMTAADLARIRANLGLDD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCC
PVPIRYVKWLGNLLKGDWGRSLTSKRPVLEEIGSRLPNTLLLMSLMLICTLLVAIPLGIL
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SAIRQYSWLDNVLTTIAFAGQSLPVFWFGLLLIIVFSVLLRGPDGKPLLPGSGMYTLGEP
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
FSLWDRIRHLILPVTMLTFVSAAEYMRYMRSAMLDVIHEDYIRTARAKGIQEWVVIYKHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNAIIPLVTLLSLDLPSLFAGALFTETIFAWPGIGRLYFTAALKNDYPLVMAILTIYSA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
LIILSNLLVDLIYAMLDPRIRLS
HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TTYLLRRLLQAVLTLFVVSLILFGLISVTPGGFMTVYAEKSDMTAADLARIRANLGLDD
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCC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HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]