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The map label for this gene is cscA [H]

Identifier: 222526191

GI number: 222526191

Start: 3644096

End: 3645589

Strand: Reverse

Name: cscA [H]

Synonym: Chy400_2950

Alternate gene names: 222526191

Gene position: 3645589-3644096 (Counterclockwise)

Preceding gene: 222526192

Following gene: 222526184

Centisome position: 69.19

GC content: 57.63

Gene sequence:

>1494_bases
ATGCACCTGGGTGATGGGCTGGGAGCACGACTGCATAGCCTTTCTGAAATAAGTTCCGACAATCTCCATGGTGAAGGTGA
TCCTATGGCAGGCGCTCAACAACGTCCGCGTTACCACTTTTTGCCGCCGGCAAACTGGATGAACGACCCAAATGGGCTTA
TTCAATGGGAAGACACGTTTCACTTGTTTTATCAGTACAACCCCGCCGGCGCATATCATCGCCATATCCATTGGGGTCAC
GCAACCAGTCACGATCTAATTCACTGGCAACATCAACCGATTGCCCTCTCACCAACGCCAGGTGGTGCTGATGCTGATGG
TTGCTGGTCGGGCTGTGCCGTTAATGACCACGGCACACCCACCCTGATCTACACCGGCTTTCGATTGCCTGAAGTACAAC
AACCGTGCCTGGCAGTCAGTCACGATGGTTTACAAACCTGGCAGAAGTGGCCAGAGCCGATCATTGCTGCACCACCGCCG
GAACTCGATCTGCTTGGCTTTCGCGACCATACTGTCTGGCAAGAACAGGGAGTCTGGCACATGCTGATCGGTTCGGGCAT
TCGTGGTCAGGGTGGTACCGTCTTGCTCTACCGCTCGCCCGATTTACGACACTGGGAATACGCAGGCCCACTCTTGATCG
GTGATGCCGGTCAGTTCGATCCGGTCTGGACGGGTTTGCTCTGGGAGTGTCCAGACTTTTTCGCGCTAGGCGACCAGCAG
GTGCTCGTCTGCTCGGCCTGGGATCGACGACCATACTACACGATTGCGATGATCGGCGCGTATCGGGATGGCCGCTTTGT
CCCGCATACAACCCACAAACTGGATTATGGCGACGCTCATTTCTACGCCCCGCAAACCATGCCGATCCGCGATGGACGAC
GCATCATGTTTGCCTGGTCAATGGAGGGACGAAGCGAGGAGTCCATTGTAGCGGCAGGTTGGGCCGGGGTGATGACGCTG
CCACGCGAGGTGAAGATAGCCGATGATGGTCAGATCGTAACATTGCCGGTGAACGAAGTCCAGCAGTTACGTCAAGACGA
GATCGTTATCCCATCAACGCGGGTCATGCCGCACGACATCCACTGGCTGCCCGTCCACGGTGCGCAGCTTGAGGTAGAGT
TAATCCTGCGCCCACCACGGCATGGAACGTGCACCGTCATGCTGCGGGCCAGCCCCGACGGCAGCGAAGCGACCGTTTTG
CGCTACAACAGCCGAACCGCAACCCTCACCCTCGACCGGCGCCGGTCGAGTCTGGCCGGTGATGTCTGGCGTGACGCTCA
CCACGCCCCCCTTACTCTGCACAACGATGAGCCGTTAGTGCTGCGCATCTTTCTTGATGCCTCGTTGATTGAAGTCTTTG
CCAACGAACGCCGTTCGATCACCAGTCGTATCTACCCAACCCGCCCTGACAGCACGGGTATTGCGATTTGTGCCGACGAG
GCTGCGGTTGATCTCCTTGCCGGACGGGTGTGGGAGATGGCCGGAATCTGGTAG

Upstream 100 bases:

>100_bases
CCAGCGTGTGATGGCACCGCAGATGGTCGGCAGCCCTCCTCGCTGCCATACCGGCAGCCTGGGAGCGGGATATGCTGGCG
TTGGCGATTGGTAAACGTTC

Downstream 100 bases:

>100_bases
CGTACATTACCACGGAAAGAAGACCGGCACCAGGATCAGACAAATCACCATCACGAGCAATTGCAGCGGGACACCAGCTC
GGACATAGTCAATAAAGCGA

Product: glycosyl hydrolase family 32 domain-containing protein

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MHLGDGLGARLHSLSEISSDNLHGEGDPMAGAQQRPRYHFLPPANWMNDPNGLIQWEDTFHLFYQYNPAGAYHRHIHWGH
ATSHDLIHWQHQPIALSPTPGGADADGCWSGCAVNDHGTPTLIYTGFRLPEVQQPCLAVSHDGLQTWQKWPEPIIAAPPP
ELDLLGFRDHTVWQEQGVWHMLIGSGIRGQGGTVLLYRSPDLRHWEYAGPLLIGDAGQFDPVWTGLLWECPDFFALGDQQ
VLVCSAWDRRPYYTIAMIGAYRDGRFVPHTTHKLDYGDAHFYAPQTMPIRDGRRIMFAWSMEGRSEESIVAAGWAGVMTL
PREVKIADDGQIVTLPVNEVQQLRQDEIVIPSTRVMPHDIHWLPVHGAQLEVELILRPPRHGTCTVMLRASPDGSEATVL
RYNSRTATLTLDRRRSSLAGDVWRDAHHAPLTLHNDEPLVLRIFLDASLIEVFANERRSITSRIYPTRPDSTGIAICADE
AAVDLLAGRVWEMAGIW

Sequences:

>Translated_497_residues
MHLGDGLGARLHSLSEISSDNLHGEGDPMAGAQQRPRYHFLPPANWMNDPNGLIQWEDTFHLFYQYNPAGAYHRHIHWGH
ATSHDLIHWQHQPIALSPTPGGADADGCWSGCAVNDHGTPTLIYTGFRLPEVQQPCLAVSHDGLQTWQKWPEPIIAAPPP
ELDLLGFRDHTVWQEQGVWHMLIGSGIRGQGGTVLLYRSPDLRHWEYAGPLLIGDAGQFDPVWTGLLWECPDFFALGDQQ
VLVCSAWDRRPYYTIAMIGAYRDGRFVPHTTHKLDYGDAHFYAPQTMPIRDGRRIMFAWSMEGRSEESIVAAGWAGVMTL
PREVKIADDGQIVTLPVNEVQQLRQDEIVIPSTRVMPHDIHWLPVHGAQLEVELILRPPRHGTCTVMLRASPDGSEATVL
RYNSRTATLTLDRRRSSLAGDVWRDAHHAPLTLHNDEPLVLRIFLDASLIEVFANERRSITSRIYPTRPDSTGIAICADE
AAVDLLAGRVWEMAGIW
>Mature_497_residues
MHLGDGLGARLHSLSEISSDNLHGEGDPMAGAQQRPRYHFLPPANWMNDPNGLIQWEDTFHLFYQYNPAGAYHRHIHWGH
ATSHDLIHWQHQPIALSPTPGGADADGCWSGCAVNDHGTPTLIYTGFRLPEVQQPCLAVSHDGLQTWQKWPEPIIAAPPP
ELDLLGFRDHTVWQEQGVWHMLIGSGIRGQGGTVLLYRSPDLRHWEYAGPLLIGDAGQFDPVWTGLLWECPDFFALGDQQ
VLVCSAWDRRPYYTIAMIGAYRDGRFVPHTTHKLDYGDAHFYAPQTMPIRDGRRIMFAWSMEGRSEESIVAAGWAGVMTL
PREVKIADDGQIVTLPVNEVQQLRQDEIVIPSTRVMPHDIHWLPVHGAQLEVELILRPPRHGTCTVMLRASPDGSEATVL
RYNSRTATLTLDRRRSSLAGDVWRDAHHAPLTLHNDEPLVLRIFLDASLIEVFANERRSITSRIYPTRPDSTGIAICADE
AAVDLLAGRVWEMAGIW

Specific function: Enables the bacterium to metabolize sucrose as a sole carbon source [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=497, Percent_Identity=22.5352112676056, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 55804; Mature: 55804

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLGDGLGARLHSLSEISSDNLHGEGDPMAGAQQRPRYHFLPPANWMNDPNGLIQWEDTF
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECEE
HLFYQYNPAGAYHRHIHWGHATSHDLIHWQHQPIALSPTPGGADADGCWSGCAVNDHGTP
EEEEEECCCCCEEEEEEECCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCEECCCCCC
TLIYTGFRLPEVQQPCLAVSHDGLQTWQKWPEPIIAAPPPELDLLGFRDHTVWQEQGVWH
EEEEECCCCCCCCCCCEEECCCCHHHHHHCCCCEEECCCCCEEEEECCCCCCHHHCCEEE
MLIGSGIRGQGGTVLLYRSPDLRHWEYAGPLLIGDAGQFDPVWTGLLWECPDFFALGDQQ
EEECCCCCCCCCEEEEEECCCCCCEECCCCEEEECCCCCCHHHHHHHHCCCCCEECCCCE
VLVCSAWDRRPYYTIAMIGAYRDGRFVPHTTHKLDYGDAHFYAPQTMPIRDGRRIMFAWS
EEEEEECCCCCEEEEEEEEECCCCEECCCCCCCCCCCCCEEECCCCCCCCCCCEEEEEEE
MEGRSEESIVAAGWAGVMTLPREVKIADDGQIVTLPVNEVQQLRQDEIVIPSTRVMPHDI
CCCCCCCCEEEECCCCEEECCCEEEEECCCEEEEECHHHHHHHHHCCEECCCCCCCCCCE
HWLPVHGAQLEVELILRPPRHGTCTVMLRASPDGSEATVLRYNSRTATLTLDRRRSSLAG
EEEEECCCEEEEEEEEECCCCCEEEEEEEECCCCCCEEEEEECCCEEEEEECCCCCHHCC
DVWRDAHHAPLTLHNDEPLVLRIFLDASLIEVFANERRSITSRIYPTRPDSTGIAICADE
HHHHCCCCCCEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECH
AAVDLLAGRVWEMAGIW
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHLGDGLGARLHSLSEISSDNLHGEGDPMAGAQQRPRYHFLPPANWMNDPNGLIQWEDTF
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECEE
HLFYQYNPAGAYHRHIHWGHATSHDLIHWQHQPIALSPTPGGADADGCWSGCAVNDHGTP
EEEEEECCCCCEEEEEEECCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCEECCCCCC
TLIYTGFRLPEVQQPCLAVSHDGLQTWQKWPEPIIAAPPPELDLLGFRDHTVWQEQGVWH
EEEEECCCCCCCCCCCEEECCCCHHHHHHCCCCEEECCCCCEEEEECCCCCCHHHCCEEE
MLIGSGIRGQGGTVLLYRSPDLRHWEYAGPLLIGDAGQFDPVWTGLLWECPDFFALGDQQ
EEECCCCCCCCCEEEEEECCCCCCEECCCCEEEECCCCCCHHHHHHHHCCCCCEECCCCE
VLVCSAWDRRPYYTIAMIGAYRDGRFVPHTTHKLDYGDAHFYAPQTMPIRDGRRIMFAWS
EEEEEECCCCCEEEEEEEEECCCCEECCCCCCCCCCCCCEEECCCCCCCCCCCEEEEEEE
MEGRSEESIVAAGWAGVMTLPREVKIADDGQIVTLPVNEVQQLRQDEIVIPSTRVMPHDI
CCCCCCCCEEEECCCCEEECCCEEEEECCCEEEEECHHHHHHHHHCCEECCCCCCCCCCE
HWLPVHGAQLEVELILRPPRHGTCTVMLRASPDGSEATVLRYNSRTATLTLDRRRSSLAG
EEEEECCCEEEEEEEEECCCCCEEEEEEEECCCCCCEEEEEECCCEEEEEECCCCCHHCC
DVWRDAHHAPLTLHNDEPLVLRIFLDASLIEVFANERRSITSRIYPTRPDSTGIAICADE
HHHHCCCCCCEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECH
AAVDLLAGRVWEMAGIW
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218016 [H]