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The map label for this gene is dagK [H]

Identifier: 222526165

GI number: 222526165

Start: 3617506

End: 3618420

Strand: Reverse

Name: dagK [H]

Synonym: Chy400_2923

Alternate gene names: 222526165

Gene position: 3618420-3617506 (Counterclockwise)

Preceding gene: 222526166

Following gene: 222526159

Centisome position: 68.67

GC content: 61.97

Gene sequence:

>915_bases
ATGACAACGACTGTCATTCTGAACCCGGCAGCCGGGCGTGGCCTGGCTGGTCGGCGGCGACACATCATCGAGGCGGAACT
ACGCAAGCACAATCTGGAATTCGAGATCTTCACCACCCATGCCCGCGGCGGTGCCACTGAACTGGCGATTCAGGCGATCA
ACCGCGGCAGCACGCAGATCGTCGCCGTAGGTGGCGATGGCACCATCAACGAGGTTGTCAACGGCATCGTCGAAGGGGGC
AAGCGCACAGCGGTCACCTTCGGCATCATCCCACTCGGCACGGGCAGTGACTTTGTCAAATCACTGCCCGGCGTCAAACC
GAACGACATCAGTGCCGCCGTTCAACGCCTGGCCGCCAACCAGACCCAGGCCATTGATGTCGGACGGATTCGGGTCACTG
CCGGACGGCTCACCCTGACCCGCTACTTTATCAATGGGCTAGGCATGGGGCTTGACGCCGCCGTCGCCGTTGAAAGCCTC
AAAATCCCCTACCTGCGTGGCTTTGCCGTCTACCTGATCTCCGTCTTACGGGCACTGGCTACCTACCGTCCCGGCCCGAT
GACCGTGCGTTTCGACGGACAGCAGATCAGCCGGCAACTCTTCTTTGCCAGTGTCGGCAACGGTCGTTGCCAGGGGGGCG
GTTTCTGGATCACCCCTGACGCCAGACTCGACGATGGTCTGCTTGATCTGTGCATTGTTGATACGATGCCGATTCCAAGG
GCACTACGCAAGATTCCGCTGCTGATGCGCGGTGTGCACGCCAACGAACCAGAAGTCACGATGGCGCGGGCACGTCGGAT
CGAGGTCACCTGCCCCACCCCCATCCCGGTCGCCACCGACGGCGAAGTCGTCGCCACCGCAGCCCAACGGGTTGAGGTAG
AGGTGGTGCCACGGGCGGTGAAGTTGCTGGTGTAA

Upstream 100 bases:

>100_bases
ATTCTGGTCAATCTGTCAGGCCGCGGTGACAAAGATATCTTTACCGTTGCCGGGTTGTTGGGGGTATCAATCTGAACTTG
AAGGGCGCGGAGCAGCGCGT

Downstream 100 bases:

>100_bases
TCCCACTTTGCGGAATGGTGGGGGGGGAGTGGGGAATGGGGAGGTGAGGGATCACCCCCACCCCAGCCCGCCCCCGCTGG
GGGCGGGAGTATACCCCCCA

Product: diacylglycerol kinase catalytic subunit

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTTTVILNPAAGRGLAGRRRHIIEAELRKHNLEFEIFTTHARGGATELAIQAINRGSTQIVAVGGDGTINEVVNGIVEGG
KRTAVTFGIIPLGTGSDFVKSLPGVKPNDISAAVQRLAANQTQAIDVGRIRVTAGRLTLTRYFINGLGMGLDAAVAVESL
KIPYLRGFAVYLISVLRALATYRPGPMTVRFDGQQISRQLFFASVGNGRCQGGGFWITPDARLDDGLLDLCIVDTMPIPR
ALRKIPLLMRGVHANEPEVTMARARRIEVTCPTPIPVATDGEVVATAAQRVEVEVVPRAVKLLV

Sequences:

>Translated_304_residues
MTTTVILNPAAGRGLAGRRRHIIEAELRKHNLEFEIFTTHARGGATELAIQAINRGSTQIVAVGGDGTINEVVNGIVEGG
KRTAVTFGIIPLGTGSDFVKSLPGVKPNDISAAVQRLAANQTQAIDVGRIRVTAGRLTLTRYFINGLGMGLDAAVAVESL
KIPYLRGFAVYLISVLRALATYRPGPMTVRFDGQQISRQLFFASVGNGRCQGGGFWITPDARLDDGLLDLCIVDTMPIPR
ALRKIPLLMRGVHANEPEVTMARARRIEVTCPTPIPVATDGEVVATAAQRVEVEVVPRAVKLLV
>Mature_303_residues
TTTVILNPAAGRGLAGRRRHIIEAELRKHNLEFEIFTTHARGGATELAIQAINRGSTQIVAVGGDGTINEVVNGIVEGGK
RTAVTFGIIPLGTGSDFVKSLPGVKPNDISAAVQRLAANQTQAIDVGRIRVTAGRLTLTRYFINGLGMGLDAAVAVESLK
IPYLRGFAVYLISVLRALATYRPGPMTVRFDGQQISRQLFFASVGNGRCQGGGFWITPDARLDDGLLDLCIVDTMPIPRA
LRKIPLLMRGVHANEPEVTMARARRIEVTCPTPIPVATDGEVVATAAQRVEVEVVPRAVKLLV

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=278, Percent_Identity=26.978417266187, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 32548; Mature: 32417

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTVILNPAAGRGLAGRRRHIIEAELRKHNLEFEIFTTHARGGATELAIQAINRGSTQI
CCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCCEEE
VAVGGDGTINEVVNGIVEGGKRTAVTFGIIPLGTGSDFVKSLPGVKPNDISAAVQRLAAN
EEECCCCCHHHHHHHHHCCCCEEEEEEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHCC
QTQAIDVGRIRVTAGRLTLTRYFINGLGMGLDAAVAVESLKIPYLRGFAVYLISVLRALA
CCCEEEECEEEEEECHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TYRPGPMTVRFDGQQISRQLFFASVGNGRCQGGGFWITPDARLDDGLLDLCIVDTMPIPR
HCCCCCEEEEECHHHHHHHEEEEECCCCEECCCCEEECCCCCCCCCCEEEEEECCCCHHH
ALRKIPLLMRGVHANEPEVTMARARRIEVTCPTPIPVATDGEVVATAAQRVEVEVVPRAV
HHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCEEEEHHHHEEEEEECHHH
KLLV
HHCC
>Mature Secondary Structure 
TTTVILNPAAGRGLAGRRRHIIEAELRKHNLEFEIFTTHARGGATELAIQAINRGSTQI
CEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCCEEE
VAVGGDGTINEVVNGIVEGGKRTAVTFGIIPLGTGSDFVKSLPGVKPNDISAAVQRLAAN
EEECCCCCHHHHHHHHHCCCCEEEEEEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHCC
QTQAIDVGRIRVTAGRLTLTRYFINGLGMGLDAAVAVESLKIPYLRGFAVYLISVLRALA
CCCEEEECEEEEEECHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TYRPGPMTVRFDGQQISRQLFFASVGNGRCQGGGFWITPDARLDDGLLDLCIVDTMPIPR
HCCCCCEEEEECHHHHHHHEEEEECCCCEECCCCEEECCCCCCCCCCEEEEEECCCCHHH
ALRKIPLLMRGVHANEPEVTMARARRIEVTCPTPIPVATDGEVVATAAQRVEVEVVPRAV
HHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCEEEEHHHHEEEEEECHHH
KLLV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA