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The map label for this gene is speE [H]

Identifier: 222526156

GI number: 222526156

Start: 3601227

End: 3602900

Strand: Direct

Name: speE [H]

Synonym: Chy400_2913

Alternate gene names: 222526156

Gene position: 3601227-3602900 (Clockwise)

Preceding gene: 222526155

Following gene: 222526157

Centisome position: 68.35

GC content: 55.73

Gene sequence:

>1674_bases
ATGGCTCAGGAAACGTTACCCTCGCACGAGACTGCAACAGTAGATGATCGGTTGCTCTTGACCGTCGTGTTTCTCGCCGG
GATTGGCACGCTCGGTATCGAGATGGTGATGCCGCGCATGCTGGCACCCTTCTTCGGCACTTCACAACCGATCTGGGCAG
TCGTTATCGGCATGACCCTGGTCTACCTGGCGATAGGCTATCGTCTGGGTGGTGCATTGGCCGACCGCCGGCCAGACTGG
CGATTGTTGTTTCAACTCATTGGCTGGGCCGGGCTGAGTTGCGCAATCATTCCCTTCATCGCCCGTCCAATCCTCGGGCT
GGCTCAGGGCGCACTACGGAACGTCGCTGCTGGCGGTTTTCTGGCAGCGCTGATCGGGGTTATCATTCTCTTTGCGGTGC
CGGTCATTCTGATGGCCATGGTTGGCCCTTTTGCGATCCGGTTGCAGTTGCGGCGGGCCGACGATGTTGAACATGCCGGA
CGGACAGCCGGTACCATCTCGGCGCTCTCAACAATTGGCTCTATTGCCGGTACCTTCCTGACGGTGCTGGTGCTGATTCC
GGCGATTGGTGTCACCAGCACGCTCTTTCTCTTTGCCGGCTTTCTGGTAGTTCTGAGTTTGATCGGTTTACGCGATCCGC
GACGCTTATTCCTGGTGTTGACGGTCATCTTACTGGCCGGAGCGTATCAACTCAACACCGGTGCAATTAAAGCTGCTGAT
TGTCGTAATTGCCGGTTAATTGCCGAATTTGAGTCGGATTACAACTACATTCAGGTTGCTGAGCAGGAAGTAACCTACAG
TGACGGCGTGGTTGATCGGCGGCGGGTCTTGATCCTCAACGAAGGGCTGGCGTTACATTCCATTTACCGGCTCAAATACC
GCGAAACGGGGGATCCGCTCGATCTCCTCACCGATGGTGGGCCGTGGGATTACTTCAGCGTTGCACCGTACCTCTACCCC
AATACCCGGCCAGAGGATGTGCGTTCACTGGCGTTGCTCGGTGCTGCCGCGGGGAGTATTGCCCAGCAATTTCTGGCTAT
CTATGGCCCTGATACCGTCATTGATGCGGTAGAGATTGATCGGAAGATCAGTGACGTGGGCAGGCGCTACTTCGACATGG
CTGATGGTACGGCCCAGGCACCCTACTTCACCACCTACAACGAAGACGCGCGCTACTGGCTGGCAACCACCGACCGCCAA
TACGACGTGATTGGGATGGATGCCTACCACCAGCCGTACATTCCATTTCACCTTACCACCGTCGAATTCTTTCGTGAAGT
CAGAGCGAAACTCACTCCTCGTGGCGTTGCGGTCGTGAATGCCGGGAGACCGGCGAGCGGTGATGACCGGCTGGTCAATG
CGCTGGCTTCAACAATGATCGCCGTCTTTCCACAGGTGTATATTATCGACACCCGCTTCAGTAATGCGATCTTAATCGGG
GTGAATCAGCCGGTTGGCGATGGGGTGATCAACTTTATCGAAAATGCCACCCAGATCGACGAACCAGCGCTGCAACTGGT
GATGTATTGGTCGTTGTACGAAGGGAGTTTCGGGCCACTACGTGAATTCACTCCGGCGATGGCGCAATTCCAACCCTTCA
CCGACGATCACGCACCGGTTGAACAACTGATTGACGGTTTAATCTTCAGTGAGGTAGGGAGAATAGATCGGTAA

Upstream 100 bases:

>100_bases
TTGAACGTGTTTTCTCGATATTTATTTGATTGACATAATAATCGAGTTTTCTGTTGGATGAGATCGGAGATGTGTTCGCC
GGACATTGTGGACATAATGC

Downstream 100 bases:

>100_bases
TGGGTTGACAAAGTAGCGAAATATATGTTACTTTTATATCGGTTACTCAGATTGTTTTATGGGAAAGCTATATTCTATGG
CGCATTCGCCGTTACAACAC

Product: spermidine synthase-like protein

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MAQETLPSHETATVDDRLLLTVVFLAGIGTLGIEMVMPRMLAPFFGTSQPIWAVVIGMTLVYLAIGYRLGGALADRRPDW
RLLFQLIGWAGLSCAIIPFIARPILGLAQGALRNVAAGGFLAALIGVIILFAVPVILMAMVGPFAIRLQLRRADDVEHAG
RTAGTISALSTIGSIAGTFLTVLVLIPAIGVTSTLFLFAGFLVVLSLIGLRDPRRLFLVLTVILLAGAYQLNTGAIKAAD
CRNCRLIAEFESDYNYIQVAEQEVTYSDGVVDRRRVLILNEGLALHSIYRLKYRETGDPLDLLTDGGPWDYFSVAPYLYP
NTRPEDVRSLALLGAAAGSIAQQFLAIYGPDTVIDAVEIDRKISDVGRRYFDMADGTAQAPYFTTYNEDARYWLATTDRQ
YDVIGMDAYHQPYIPFHLTTVEFFREVRAKLTPRGVAVVNAGRPASGDDRLVNALASTMIAVFPQVYIIDTRFSNAILIG
VNQPVGDGVINFIENATQIDEPALQLVMYWSLYEGSFGPLREFTPAMAQFQPFTDDHAPVEQLIDGLIFSEVGRIDR

Sequences:

>Translated_557_residues
MAQETLPSHETATVDDRLLLTVVFLAGIGTLGIEMVMPRMLAPFFGTSQPIWAVVIGMTLVYLAIGYRLGGALADRRPDW
RLLFQLIGWAGLSCAIIPFIARPILGLAQGALRNVAAGGFLAALIGVIILFAVPVILMAMVGPFAIRLQLRRADDVEHAG
RTAGTISALSTIGSIAGTFLTVLVLIPAIGVTSTLFLFAGFLVVLSLIGLRDPRRLFLVLTVILLAGAYQLNTGAIKAAD
CRNCRLIAEFESDYNYIQVAEQEVTYSDGVVDRRRVLILNEGLALHSIYRLKYRETGDPLDLLTDGGPWDYFSVAPYLYP
NTRPEDVRSLALLGAAAGSIAQQFLAIYGPDTVIDAVEIDRKISDVGRRYFDMADGTAQAPYFTTYNEDARYWLATTDRQ
YDVIGMDAYHQPYIPFHLTTVEFFREVRAKLTPRGVAVVNAGRPASGDDRLVNALASTMIAVFPQVYIIDTRFSNAILIG
VNQPVGDGVINFIENATQIDEPALQLVMYWSLYEGSFGPLREFTPAMAQFQPFTDDHAPVEQLIDGLIFSEVGRIDR
>Mature_556_residues
AQETLPSHETATVDDRLLLTVVFLAGIGTLGIEMVMPRMLAPFFGTSQPIWAVVIGMTLVYLAIGYRLGGALADRRPDWR
LLFQLIGWAGLSCAIIPFIARPILGLAQGALRNVAAGGFLAALIGVIILFAVPVILMAMVGPFAIRLQLRRADDVEHAGR
TAGTISALSTIGSIAGTFLTVLVLIPAIGVTSTLFLFAGFLVVLSLIGLRDPRRLFLVLTVILLAGAYQLNTGAIKAADC
RNCRLIAEFESDYNYIQVAEQEVTYSDGVVDRRRVLILNEGLALHSIYRLKYRETGDPLDLLTDGGPWDYFSVAPYLYPN
TRPEDVRSLALLGAAAGSIAQQFLAIYGPDTVIDAVEIDRKISDVGRRYFDMADGTAQAPYFTTYNEDARYWLATTDRQY
DVIGMDAYHQPYIPFHLTTVEFFREVRAKLTPRGVAVVNAGRPASGDDRLVNALASTMIAVFPQVYIIDTRFSNAILIGV
NQPVGDGVINFIENATQIDEPALQLVMYWSLYEGSFGPLREFTPAMAQFQPFTDDHAPVEQLIDGLIFSEVGRIDR

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 61070; Mature: 60938

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQETLPSHETATVDDRLLLTVVFLAGIGTLGIEMVMPRMLAPFFGTSQPIWAVVIGMTL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VYLAIGYRLGGALADRRPDWRLLFQLIGWAGLSCAIIPFIARPILGLAQGALRNVAAGGF
HHHHHHHHHCCHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LAALIGVIILFAVPVILMAMVGPFAIRLQLRRADDVEHAGRTAGTISALSTIGSIAGTFL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
TVLVLIPAIGVTSTLFLFAGFLVVLSLIGLRDPRRLFLVLTVILLAGAYQLNTGAIKAAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECCCCEEECC
CRNCRLIAEFESDYNYIQVAEQEVTYSDGVVDRRRVLILNEGLALHSIYRLKYRETGDPL
CCCCEEEEEECCCCCEEEEEHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCH
DLLTDGGPWDYFSVAPYLYPNTRPEDVRSLALLGAAAGSIAQQFLAIYGPDTVIDAVEID
HEECCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RKISDVGRRYFDMADGTAQAPYFTTYNEDARYWLATTDRQYDVIGMDAYHQPYIPFHLTT
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCCEEHHH
VEFFREVRAKLTPRGVAVVNAGRPASGDDRLVNALASTMIAVFPQVYIIDTRFSNAILIG
HHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEE
VNQPVGDGVINFIENATQIDEPALQLVMYWSLYEGSFGPLREFTPAMAQFQPFTDDHAPV
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHCHHHHHCCCCCCCCCHH
EQLIDGLIFSEVGRIDR
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AQETLPSHETATVDDRLLLTVVFLAGIGTLGIEMVMPRMLAPFFGTSQPIWAVVIGMTL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VYLAIGYRLGGALADRRPDWRLLFQLIGWAGLSCAIIPFIARPILGLAQGALRNVAAGGF
HHHHHHHHHCCHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LAALIGVIILFAVPVILMAMVGPFAIRLQLRRADDVEHAGRTAGTISALSTIGSIAGTFL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
TVLVLIPAIGVTSTLFLFAGFLVVLSLIGLRDPRRLFLVLTVILLAGAYQLNTGAIKAAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECCCCEEECC
CRNCRLIAEFESDYNYIQVAEQEVTYSDGVVDRRRVLILNEGLALHSIYRLKYRETGDPL
CCCCEEEEEECCCCCEEEEEHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCH
DLLTDGGPWDYFSVAPYLYPNTRPEDVRSLALLGAAAGSIAQQFLAIYGPDTVIDAVEID
HEECCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RKISDVGRRYFDMADGTAQAPYFTTYNEDARYWLATTDRQYDVIGMDAYHQPYIPFHLTT
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCEEEEECCCCCCCCCCEEHHH
VEFFREVRAKLTPRGVAVVNAGRPASGDDRLVNALASTMIAVFPQVYIIDTRFSNAILIG
HHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEE
VNQPVGDGVINFIENATQIDEPALQLVMYWSLYEGSFGPLREFTPAMAQFQPFTDDHAPV
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHCHHHHHCCCCCCCCCHH
EQLIDGLIFSEVGRIDR
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]