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The map label for this gene is dnaN [H]

Identifier: 222526095

GI number: 222526095

Start: 3522413

End: 3523546

Strand: Reverse

Name: dnaN [H]

Synonym: Chy400_2852

Alternate gene names: 222526095

Gene position: 3523546-3522413 (Counterclockwise)

Preceding gene: 222526104

Following gene: 222526094

Centisome position: 66.87

GC content: 53.09

Gene sequence:

>1134_bases
ATGAAGCTCACATGCATGCAGGAAGATTTAAAGCGTGGTCTGGCCGCAGTTGGCCATGCCGTCGCCGGTAAGAGCACTCT
GCCAGTGTTGGCGAATATACTCCTTGCCACCGATGAAGGTCGGCTGAAGTTGACGGCTACCAATCTGGAGGTAGGTATTA
ATCGCTGGATCAGCGCTACGATTACTCGCGACGGTGCAGTAGCCGTACCGGCCAAATTATTAACTGATGTGGTCGGCGGC
CTGCCAAATGATAAGGTAACACTAAGCCTCGATCAGAAAACCCAGACCTTACGTGTTGAGTGCGGGCGTTTCGTGAGCAA
TATTAAAGGGGTGGATGCTGACGAATTTCCTTCGCTACCAACTGTTAGCGCACAAACGCCGCTGGTATCACTACCGGCTG
AGGTATGGCAAGAGGCGATTGATCAGGTGGCGTTTGCGGCTGCCACTGATGAGTCGCGTCCGATCCTGACCGGGGTGCTG
GTACGCACGCGCGATCAGCAAGTCACGCTGGTTGCGGCGGATGGCTTCCGTCTGGCGATGCGAACAATTCAGTTGTCTGC
TCCGGTTGCGCACGCGGTTGATTGCCTGATCCCGGCGCGTACATTGAGCGAATTGGCCCGGATTATTGGTGATACGGACG
CAGAAGTGGCAATGGTCTATACGCCAGGGGGCAGCCACGTGCTCTTCCACACCGAAAATATTGAAGTCGTATCACGTTTA
ATAGAAGGAAGATTTCCTGATTTCGAGCGGATTATCCCGCAGCAGTACCTGACACGCATGGTTCTTGATAACAGTGAACT
GGCGAAGGCGGTCAAGCTGGCATCATTCTTCGCCGGCAGTAGCCAGAATGTGGTGAAGCTGAAGATTGAACCGGCAAGTG
AATTAGCACCGGGACGGGTTGTGATCAGTGCAAATGCTGCTGAGTTGGGTGATAATACCAGTGAACTTGATGGCAGTGTG
ACCGGTGAAGGCGGTGTTATTGCGCTCAATGTCAGATTCCTGGCCGATGCGATTGCAGCAGTACACTCCAATCAGATCGC
CTTTGAAATGCAGAGTGCGCAAAGTCCGGCAGTGTTTAAGCCGGTCGGTCAAGACGGGTACATTCATATTGTGATGCCAA
TGAGTATGCGATAG

Upstream 100 bases:

>100_bases
ACGCTGTCATTGGTTAGCGCAATATGCTATAATGCGCCACACCCGGATCAAGGGCGGCGTTCGTATGCCGTGATTGAGGT
TGTCTTTTCAAGGAGCGACA

Downstream 100 bases:

>100_bases
ATGGTATCGGTGGCAGAGCCGACATCAACAAATCGGTGGAGGAGTTACCCGTGAGTGTGACAACGCTGATAATTGTGCAT
ACTGTCTCTCTCTTTCGAGA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKLTCMQEDLKRGLAAVGHAVAGKSTLPVLANILLATDEGRLKLTATNLEVGINRWISATITRDGAVAVPAKLLTDVVGG
LPNDKVTLSLDQKTQTLRVECGRFVSNIKGVDADEFPSLPTVSAQTPLVSLPAEVWQEAIDQVAFAAATDESRPILTGVL
VRTRDQQVTLVAADGFRLAMRTIQLSAPVAHAVDCLIPARTLSELARIIGDTDAEVAMVYTPGGSHVLFHTENIEVVSRL
IEGRFPDFERIIPQQYLTRMVLDNSELAKAVKLASFFAGSSQNVVKLKIEPASELAPGRVVISANAAELGDNTSELDGSV
TGEGGVIALNVRFLADAIAAVHSNQIAFEMQSAQSPAVFKPVGQDGYIHIVMPMSMR

Sequences:

>Translated_377_residues
MKLTCMQEDLKRGLAAVGHAVAGKSTLPVLANILLATDEGRLKLTATNLEVGINRWISATITRDGAVAVPAKLLTDVVGG
LPNDKVTLSLDQKTQTLRVECGRFVSNIKGVDADEFPSLPTVSAQTPLVSLPAEVWQEAIDQVAFAAATDESRPILTGVL
VRTRDQQVTLVAADGFRLAMRTIQLSAPVAHAVDCLIPARTLSELARIIGDTDAEVAMVYTPGGSHVLFHTENIEVVSRL
IEGRFPDFERIIPQQYLTRMVLDNSELAKAVKLASFFAGSSQNVVKLKIEPASELAPGRVVISANAAELGDNTSELDGSV
TGEGGVIALNVRFLADAIAAVHSNQIAFEMQSAQSPAVFKPVGQDGYIHIVMPMSMR
>Mature_377_residues
MKLTCMQEDLKRGLAAVGHAVAGKSTLPVLANILLATDEGRLKLTATNLEVGINRWISATITRDGAVAVPAKLLTDVVGG
LPNDKVTLSLDQKTQTLRVECGRFVSNIKGVDADEFPSLPTVSAQTPLVSLPAEVWQEAIDQVAFAAATDESRPILTGVL
VRTRDQQVTLVAADGFRLAMRTIQLSAPVAHAVDCLIPARTLSELARIIGDTDAEVAMVYTPGGSHVLFHTENIEVVSRL
IEGRFPDFERIIPQQYLTRMVLDNSELAKAVKLASFFAGSSQNVVKLKIEPASELAPGRVVISANAAELGDNTSELDGSV
TGEGGVIALNVRFLADAIAAVHSNQIAFEMQSAQSPAVFKPVGQDGYIHIVMPMSMR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=380, Percent_Identity=25.5263157894737, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40227; Mature: 40227

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTCMQEDLKRGLAAVGHAVAGKSTLPVLANILLATDEGRLKLTATNLEVGINRWISAT
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCEEEEEECEEECCCCEEEEE
ITRDGAVAVPAKLLTDVVGGLPNDKVTLSLDQKTQTLRVECGRFVSNIKGVDADEFPSLP
EECCCCEECHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHCCCCC
TVSAQTPLVSLPAEVWQEAIDQVAFAAATDESRPILTGVLVRTRDQQVTLVAADGFRLAM
CCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEEEEECCCEEEE
RTIQLSAPVAHAVDCLIPARTLSELARIIGDTDAEVAMVYTPGGSHVLFHTENIEVVSRL
HEEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCHHHHHHH
IEGRFPDFERIIPQQYLTRMVLDNSELAKAVKLASFFAGSSQNVVKLKIEPASELAPGRV
HHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHCCCCCEE
VISANAAELGDNTSELDGSVTGEGGVIALNVRFLADAIAAVHSNQIAFEMQSAQSPAVFK
EEEECHHHCCCCHHHCCCEECCCCCEEEEEHHHHHHHHHHHHCCCEEEEECCCCCCCEEE
PVGQDGYIHIVMPMSMR
ECCCCCEEEEEEECCCC
>Mature Secondary Structure
MKLTCMQEDLKRGLAAVGHAVAGKSTLPVLANILLATDEGRLKLTATNLEVGINRWISAT
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCEEEEEECEEECCCCEEEEE
ITRDGAVAVPAKLLTDVVGGLPNDKVTLSLDQKTQTLRVECGRFVSNIKGVDADEFPSLP
EECCCCEECHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHCCCCC
TVSAQTPLVSLPAEVWQEAIDQVAFAAATDESRPILTGVLVRTRDQQVTLVAADGFRLAM
CCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCEEEEEEECCCEEEE
RTIQLSAPVAHAVDCLIPARTLSELARIIGDTDAEVAMVYTPGGSHVLFHTENIEVVSRL
HEEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCHHHHHHH
IEGRFPDFERIIPQQYLTRMVLDNSELAKAVKLASFFAGSSQNVVKLKIEPASELAPGRV
HHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHCCCCCEE
VISANAAELGDNTSELDGSVTGEGGVIALNVRFLADAIAAVHSNQIAFEMQSAQSPAVFK
EEEECHHHCCCCHHHCCCEECCCCCEEEEEHHHHHHHHHHHHCCCEEEEECCCCCCCEEE
PVGQDGYIHIVMPMSMR
ECCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]