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The map label for this gene is clpB [H]

Identifier: 222525912

GI number: 222525912

Start: 3297795

End: 3300296

Strand: Reverse

Name: clpB [H]

Synonym: Chy400_2667

Alternate gene names: 222525912

Gene position: 3300296-3297795 (Counterclockwise)

Preceding gene: 222525913

Following gene: 222525904

Centisome position: 62.64

GC content: 60.31

Gene sequence:

>2502_bases
ATGTCTGACCTCTACAGCAAATTTACCAAGCGCGCCAAGCAAGTGCTGCAACTGGCAGCCGAGGAAGCACGTGCGTTCAA
TCACCCATACATTGGTACCGAGCATATTTTGCTGGGCTTGATCCGCGAAAGTGAAGGGATTGCTGCGCGCGTCCTCGATG
AGCTGGGTGTGAAATTGCCCCAGGCCCGTAGTGCGGTCGAGTTTATCGTTGGCCCTGGCGAAAGCACAATGGTTACCGAC
CAGGAGCTGACGGCACGGGCGCAGAAGGTGATTAAGTATGCTATCGAAGAGGCGAATCGCCTCAACCATCACTACATCGG
CACCGAGCATCTGCTGCTGGGGTTGGTGCGCAACGGTGAGGGGGTGGCAACCGGTGTCCTCGATATTCTCGGTGTCTCCC
TCGAACAGGTGCGCAATCAGGTAATGCGTGTCTTGCGCCACGGCACAGCCGGCGCAACTGCTGAAGGACGGACTGCTCCC
GGTACGACGCCGACGCAGCGGCAGTCGAAGACCCCGTATCTCGACGCACTGGGTACCGATTTGACCGAGATGGCCGAGCA
GGGTCGTCTCGACCCCATCATTGGCCGCCAACACGAGATCGAGCGCGTCATTCAGATTCTCTCACGCCGCACGAAGAACA
ATCCGGCCCTGATTGGTGAACCGGGTGTCGGTAAGACAGCCATCGTCGAGGGCCTCGCCCAGCGCATCGTTCAGGGTGAT
GTGCCAGATAGCATTAAGGGCAAGCGAGTTGTTACGCTCGACATGGGTGCGCTGGTTGCCGGTACCAAGTACCGTGGTCA
ATTTGAAGAGCGGTTGAAGCGCGTGATCGACGAGATCAAGGAGTCGCGCTGTATTCTCTTTATCGATGAGTTCCACACCA
TCATCGGTGCCGGTGGCGCAGAAGGGACGCTCGATGCGGCCAACATCCTCAAGCCCGCTCTGTCGCGTGGAGAGTTGCAA
ACCATCGGGGCCACAACCCTCGATGAGTACCGCAAGTATATCGAGCGTGACGCCGCTCTGGAACGCCGCTTCCAGCCGGT
GATGGTTGATGAGCCGTCGATTGAAGATACCATCCAGATTTTGCGCGGGATCAAGTCGCGCTACGAGGATTTCCATCAGT
TGCAGATCAGTGACGAGGCGATCCGGGCAGCGGCAGTACTCTCGGCACGCTACGTCCCTGACCGCCAGCTCCCCGATAAG
GCGATTGACCTGATTGACGAAGCGGCGTCACGGGTACGCATGTACCGCTCGGCGACACCGCCGCGCCTGCGCGATGCCCT
GCGTGGCCTGGAGGCGCTGCGCAAAGAGCGCGAAGCCGCCCTCGAAGATCAGCAGTTTGAACTTGCCAACGATCTGCGCG
AGCGCGAAGAGCGTATGCTCAGCCGCATCCAGGAGATCGAGGCCGAGCTGGGTACCCGCAGCGACGAACGCTACGAGCGC
CCATACGTCACCGAAGAAGACATTGCCGAGGTCGTTGGCATGTGGACGGGCGTGCCCGTGACCCGGCTGACCGGTAATGA
AACCCAGCGCCTGATGCAGATGGAGGAGTTCCTCCATAGCCGGATCGTCGGTCAGCACGAAGCCATCGTCACCATCTCGA
AAGCCGTGCGTCGCGCCCGTGCCGGCCTGAAAGACCCGAAGCGCCCAATCGGAAGCTTCATCTTCCTCGGCCCAACCGGT
GTCGGCAAGACCGAGCTGGCCAAGGCACTGGCCGAGTTTATGTTCGGCACCGAAGAGGCGCTGATCAAGATCGACATGTC
AGAGTTCCAGGAGCGCCACACGACCTCGCGCCTGGTTGGCTCGCCGCCCGGCTACATCGGCTACGGCGAAGGTGGTCAAC
TGACCGATGCCGTGCGCCGGAAGCCGTACAGTGTCGTGCTGTTTGATGAAATCGAAAAGGCGCACCCCGATGCCTTCAAC
CTGCTGCTCCAGGTGCTGGAGGATGGTCATCTGACCGATGGTAAAGGGCGACGGGTCGACTTCCGCAATACCATCATCAT
CATGACCAGCAACGTCGGCACCGAACACATTCGCCGCGCATCGCGGATTGGGTTTGGTAGCAACGCCGCCGAGATCGATC
AGAACGATATGCGGCGCAAGGTGGATGAAGCGCTCAGAATGCTCTTCCGGCCAGAGTTCCTCAACCGGATCGACGCGACC
ATCATCTTCCACCCGCTCACCACCGAGGAGATTCAATCGATCACCCGGCTCATGCTCAAGCGTGTCCAGACCCAGCTCGA
CGAGCACCAGATCAAGCTGGAGGTGCAACCCGACGCCCTCGAATTCCTGGCCCGCCGCGGCTACGATCCGGCGTTCGGTG
CCCGCCCCTTGCGCCGGGTGATCACCAACCTGATCGAAGACCCGCTGGCGGAGGGGCTGCTCTCTGGTCAGTTCCGCGAC
GGCGATACAGTTATCGTCGATACCTTTGAGGATCAGTTGCGCCTCCGTTCACAACGCGAGATCGACCAGGAACACGTCGA
GGAAAGCCCGGCACTTGCCTGA

Upstream 100 bases:

>100_bases
CCTGGTCGGTGTTTCAGATAAAACAACGGGTGTTGTTCGCTATCCCCGGTCTGTCAAAGCCTGATAATCGTTTCCTCCTA
TTGAGTGAGCGGAGAATCAT

Downstream 100 bases:

>100_bases
GCGGTAAGGTAGAGACGAAGCGAGGAGCCGTAGAGGCTCCTCGCTTTGGGTTTGGTGAACTCTTGCTTTTCACGGCTGGC
CTGGCGCGCTTGCACCGCAA

Product: ATPase AAA-2 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 833; Mature: 832

Protein sequence:

>833_residues
MSDLYSKFTKRAKQVLQLAAEEARAFNHPYIGTEHILLGLIRESEGIAARVLDELGVKLPQARSAVEFIVGPGESTMVTD
QELTARAQKVIKYAIEEANRLNHHYIGTEHLLLGLVRNGEGVATGVLDILGVSLEQVRNQVMRVLRHGTAGATAEGRTAP
GTTPTQRQSKTPYLDALGTDLTEMAEQGRLDPIIGRQHEIERVIQILSRRTKNNPALIGEPGVGKTAIVEGLAQRIVQGD
VPDSIKGKRVVTLDMGALVAGTKYRGQFEERLKRVIDEIKESRCILFIDEFHTIIGAGGAEGTLDAANILKPALSRGELQ
TIGATTLDEYRKYIERDAALERRFQPVMVDEPSIEDTIQILRGIKSRYEDFHQLQISDEAIRAAAVLSARYVPDRQLPDK
AIDLIDEAASRVRMYRSATPPRLRDALRGLEALRKEREAALEDQQFELANDLREREERMLSRIQEIEAELGTRSDERYER
PYVTEEDIAEVVGMWTGVPVTRLTGNETQRLMQMEEFLHSRIVGQHEAIVTISKAVRRARAGLKDPKRPIGSFIFLGPTG
VGKTELAKALAEFMFGTEEALIKIDMSEFQERHTTSRLVGSPPGYIGYGEGGQLTDAVRRKPYSVVLFDEIEKAHPDAFN
LLLQVLEDGHLTDGKGRRVDFRNTIIIMTSNVGTEHIRRASRIGFGSNAAEIDQNDMRRKVDEALRMLFRPEFLNRIDAT
IIFHPLTTEEIQSITRLMLKRVQTQLDEHQIKLEVQPDALEFLARRGYDPAFGARPLRRVITNLIEDPLAEGLLSGQFRD
GDTVIVDTFEDQLRLRSQREIDQEHVEESPALA

Sequences:

>Translated_833_residues
MSDLYSKFTKRAKQVLQLAAEEARAFNHPYIGTEHILLGLIRESEGIAARVLDELGVKLPQARSAVEFIVGPGESTMVTD
QELTARAQKVIKYAIEEANRLNHHYIGTEHLLLGLVRNGEGVATGVLDILGVSLEQVRNQVMRVLRHGTAGATAEGRTAP
GTTPTQRQSKTPYLDALGTDLTEMAEQGRLDPIIGRQHEIERVIQILSRRTKNNPALIGEPGVGKTAIVEGLAQRIVQGD
VPDSIKGKRVVTLDMGALVAGTKYRGQFEERLKRVIDEIKESRCILFIDEFHTIIGAGGAEGTLDAANILKPALSRGELQ
TIGATTLDEYRKYIERDAALERRFQPVMVDEPSIEDTIQILRGIKSRYEDFHQLQISDEAIRAAAVLSARYVPDRQLPDK
AIDLIDEAASRVRMYRSATPPRLRDALRGLEALRKEREAALEDQQFELANDLREREERMLSRIQEIEAELGTRSDERYER
PYVTEEDIAEVVGMWTGVPVTRLTGNETQRLMQMEEFLHSRIVGQHEAIVTISKAVRRARAGLKDPKRPIGSFIFLGPTG
VGKTELAKALAEFMFGTEEALIKIDMSEFQERHTTSRLVGSPPGYIGYGEGGQLTDAVRRKPYSVVLFDEIEKAHPDAFN
LLLQVLEDGHLTDGKGRRVDFRNTIIIMTSNVGTEHIRRASRIGFGSNAAEIDQNDMRRKVDEALRMLFRPEFLNRIDAT
IIFHPLTTEEIQSITRLMLKRVQTQLDEHQIKLEVQPDALEFLARRGYDPAFGARPLRRVITNLIEDPLAEGLLSGQFRD
GDTVIVDTFEDQLRLRSQREIDQEHVEESPALA
>Mature_832_residues
SDLYSKFTKRAKQVLQLAAEEARAFNHPYIGTEHILLGLIRESEGIAARVLDELGVKLPQARSAVEFIVGPGESTMVTDQ
ELTARAQKVIKYAIEEANRLNHHYIGTEHLLLGLVRNGEGVATGVLDILGVSLEQVRNQVMRVLRHGTAGATAEGRTAPG
TTPTQRQSKTPYLDALGTDLTEMAEQGRLDPIIGRQHEIERVIQILSRRTKNNPALIGEPGVGKTAIVEGLAQRIVQGDV
PDSIKGKRVVTLDMGALVAGTKYRGQFEERLKRVIDEIKESRCILFIDEFHTIIGAGGAEGTLDAANILKPALSRGELQT
IGATTLDEYRKYIERDAALERRFQPVMVDEPSIEDTIQILRGIKSRYEDFHQLQISDEAIRAAAVLSARYVPDRQLPDKA
IDLIDEAASRVRMYRSATPPRLRDALRGLEALRKEREAALEDQQFELANDLREREERMLSRIQEIEAELGTRSDERYERP
YVTEEDIAEVVGMWTGVPVTRLTGNETQRLMQMEEFLHSRIVGQHEAIVTISKAVRRARAGLKDPKRPIGSFIFLGPTGV
GKTELAKALAEFMFGTEEALIKIDMSEFQERHTTSRLVGSPPGYIGYGEGGQLTDAVRRKPYSVVLFDEIEKAHPDAFNL
LLQVLEDGHLTDGKGRRVDFRNTIIIMTSNVGTEHIRRASRIGFGSNAAEIDQNDMRRKVDEALRMLFRPEFLNRIDATI
IFHPLTTEEIQSITRLMLKRVQTQLDEHQIKLEVQPDALEFLARRGYDPAFGARPLRRVITNLIEDPLAEGLLSGQFRDG
DTVIVDTFEDQLRLRSQREIDQEHVEESPALA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=298, Percent_Identity=37.9194630872483, Blast_Score=194, Evalue=3e-49,
Organism=Escherichia coli, GI1788943, Length=863, Percent_Identity=46.4658169177289, Blast_Score=731, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=408, Percent_Identity=40.4411764705882, Blast_Score=293, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6320464, Length=706, Percent_Identity=48.300283286119, Blast_Score=626, Evalue=1e-180,
Organism=Saccharomyces cerevisiae, GI6323002, Length=892, Percent_Identity=37.219730941704, Blast_Score=533, Evalue=1e-152,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- InterPro:   IPR001943 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 93476; Mature: 93345

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00870 CLPAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLYSKFTKRAKQVLQLAAEEARAFNHPYIGTEHILLGLIRESEGIAARVLDELGVKLP
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCC
QARSAVEFIVGPGESTMVTDQELTARAQKVIKYAIEEANRLNHHYIGTEHLLLGLVRNGE
CHHHHHHEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
GVATGVLDILGVSLEQVRNQVMRVLRHGTAGATAEGRTAPGTTPTQRQSKTPYLDALGTD
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCC
LTEMAEQGRLDPIIGRQHEIERVIQILSRRTKNNPALIGEPGVGKTAIVEGLAQRIVQGD
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHCCC
VPDSIKGKRVVTLDMGALVAGTKYRGQFEERLKRVIDEIKESRCILFIDEFHTIIGAGGA
CCCCCCCCEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCC
EGTLDAANILKPALSRGELQTIGATTLDEYRKYIERDAALERRFQPVMVDEPSIEDTIQI
CCCCCHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
LRGIKSRYEDFHQLQISDEAIRAAAVLSARYVPDRQLPDKAIDLIDEAASRVRMYRSATP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PRLRDALRGLEALRKEREAALEDQQFELANDLREREERMLSRIQEIEAELGTRSDERYER
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCC
PYVTEEDIAEVVGMWTGVPVTRLTGNETQRLMQMEEFLHSRIVGQHEAIVTISKAVRRAR
CCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AGLKDPKRPIGSFIFLGPTGVGKTELAKALAEFMFGTEEALIKIDMSEFQERHTTSRLVG
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHCC
SPPGYIGYGEGGQLTDAVRRKPYSVVLFDEIEKAHPDAFNLLLQVLEDGHLTDGKGRRVD
CCCCCCCCCCCCCHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEE
FRNTIIIMTSNVGTEHIRRASRIGFGSNAAEIDQNDMRRKVDEALRMLFRPEFLNRIDAT
ECCEEEEEECCCCHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHCHHHHHHHCCE
IIFHPLTTEEIQSITRLMLKRVQTQLDEHQIKLEVQPDALEFLARRGYDPAFGARPLRRV
EEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCCCCHHHHHH
ITNLIEDPLAEGLLSGQFRDGDTVIVDTFEDQLRLRSQREIDQEHVEESPALA
HHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDLYSKFTKRAKQVLQLAAEEARAFNHPYIGTEHILLGLIRESEGIAARVLDELGVKLP
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCC
QARSAVEFIVGPGESTMVTDQELTARAQKVIKYAIEEANRLNHHYIGTEHLLLGLVRNGE
CHHHHHHEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
GVATGVLDILGVSLEQVRNQVMRVLRHGTAGATAEGRTAPGTTPTQRQSKTPYLDALGTD
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCC
LTEMAEQGRLDPIIGRQHEIERVIQILSRRTKNNPALIGEPGVGKTAIVEGLAQRIVQGD
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHCCC
VPDSIKGKRVVTLDMGALVAGTKYRGQFEERLKRVIDEIKESRCILFIDEFHTIIGAGGA
CCCCCCCCEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCC
EGTLDAANILKPALSRGELQTIGATTLDEYRKYIERDAALERRFQPVMVDEPSIEDTIQI
CCCCCHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
LRGIKSRYEDFHQLQISDEAIRAAAVLSARYVPDRQLPDKAIDLIDEAASRVRMYRSATP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PRLRDALRGLEALRKEREAALEDQQFELANDLREREERMLSRIQEIEAELGTRSDERYER
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCC
PYVTEEDIAEVVGMWTGVPVTRLTGNETQRLMQMEEFLHSRIVGQHEAIVTISKAVRRAR
CCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AGLKDPKRPIGSFIFLGPTGVGKTELAKALAEFMFGTEEALIKIDMSEFQERHTTSRLVG
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHCC
SPPGYIGYGEGGQLTDAVRRKPYSVVLFDEIEKAHPDAFNLLLQVLEDGHLTDGKGRRVD
CCCCCCCCCCCCCHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEE
FRNTIIIMTSNVGTEHIRRASRIGFGSNAAEIDQNDMRRKVDEALRMLFRPEFLNRIDAT
ECCEEEEEECCCCHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHCHHHHHHHCCE
IIFHPLTTEEIQSITRLMLKRVQTQLDEHQIKLEVQPDALEFLARRGYDPAFGARPLRRV
EEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCCCCHHHHHH
ITNLIEDPLAEGLLSGQFRDGDTVIVDTFEDQLRLRSQREIDQEHVEESPALA
HHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]