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The map label for this gene is miaB

Identifier: 222525848

GI number: 222525848

Start: 3237526

End: 3238926

Strand: Reverse

Name: miaB

Synonym: Chy400_2601

Alternate gene names: 222525848

Gene position: 3238926-3237526 (Counterclockwise)

Preceding gene: 222525849

Following gene: 222525847

Centisome position: 61.47

GC content: 57.39

Gene sequence:

>1401_bases
ATGCAGGACGTGATCTTTTTCGACCAGCGCCCCCGTCCAGATCGTGATCAGACGCCGCGCGAGCGGCGCTATTACGTCTG
GACTGTTGGGTGTCAGATGAATATCTCAGACTCTGAACGGCTTGAAGCTGCGCTGCAGGGTGTGGGCTATAGCCCGGCAA
CCCGTCCTGAAGATGCCAGCTTTATTGTGCTGAATTCGTGCAGTGTGCGCGCTTCAGCCGAAGAGCGTATCCTTGGTAAG
CTCGGTGAGTTGGTCCGGGTTAAGCGGCAGCACCCCGATACCCGGATTGTACTCTGGGGTTGTATGGTTGGCCCCAATAA
TCGCTCCATCTTCGCCGATCAATTACCGATGGTTGATCACTTTGTTTCACCTTCGGCTGTCGATGAGGTGGTAGCCCTGG
CGCCTAACCCCATCTACACGCTCGACGAGCCGGCATTGCCCGTGCGTGACTGGAGCCATCCGCCGGTATCGGTGCATGTC
CCCATTCAGTACGGTTGCAATATGACTTGCTCCTACTGCGTGATTCCGCTGCGCCGGGGTCGTGAACGCTCGCGACCACT
CGCCGAGATTGTCGAAGAGGTGCGCCGTATTGTGGCTCGTGGCGCGAAAGAAATCACGCTCCTCGGCCAGATTGTCGATT
CCTGGGGGCACGATTTGCCCGGTCGCCCTGAACTGGCTGACTTGCTTGAGGCTGTTGATCCCACGCCGGGTTTGCTGCGC
CTGCGCTTCCTGACCTCACATCCGGCCTGGATGACTGACCGTCTGATCGAGACGGTTGCCCGCTTGCCGCGTTGTCAGCC
AGAGATTAATCTACCGGTACAGGCTGGTTCTGACCGGGTCCTCAAGCTGATGCGTCGTGGCTATACGGTTGCTCGCTACA
AGACGCTGATTGCCAGGATTCGCGCTGCGATCCCTGACATTTCACTGACGACCGACATTATCGTTGGTCATCCTGGCGAA
ACCGAGGATGATTTCCGTCAGACGATGGATCTGTGTGCTGAAATCGGTTTTGATAAAGTCCATATCGCGGCCTTCTCTGC
CCGTCCCGGTACACGGGCTGCCGAACAGGAGCAAGACCCGGCCCTCGCTGTACCACCTGCCGTGAAAGAAGAACGCCGCC
GCCGGCTCGAACAGTTGCAAGAGCAGATTGCTACCGAACGCATGGCTCGCTTCCTCGGCCAAACAGTAGAGGTATTGGTT
GAGGGTGAAAGCAAGGGGAAATGGCGTGGTCGTACCCCTGGCAACCGCCTGGTCTTCTTCAGTCACCCTGCCGATCTAAC
CGGTCAGTTGGTGCCGGTGAAGATCACCGCTACCAGCCCATGGTCGTTGCAGGGAGTGCCGCAATTAGATGATTCTGTTA
ATGCTCAATCCAAGGAGAATGGCCTTGTCAAAGCAAGATGA

Upstream 100 bases:

>100_bases
TCACCACGTTCGGACGCTTTCCGCTGCCTTGAGTCGCTCCGCGAGGCAGCTCGCCAAAGCGTCGAGTGTGGCTTACCCGT
CATCGTTTGGTAAGGGTTCC

Downstream 100 bases:

>100_bases
AAAGATGCGCATTCGCCGCCGCTTGCAAGAAAAAGCCATTGAACTGGCTGCCCTTAACCGCTGGCAAGAGGCGGCTGATT
TGAACCAACAATTGTTGCGG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MQDVIFFDQRPRPDRDQTPRERRYYVWTVGCQMNISDSERLEAALQGVGYSPATRPEDASFIVLNSCSVRASAEERILGK
LGELVRVKRQHPDTRIVLWGCMVGPNNRSIFADQLPMVDHFVSPSAVDEVVALAPNPIYTLDEPALPVRDWSHPPVSVHV
PIQYGCNMTCSYCVIPLRRGRERSRPLAEIVEEVRRIVARGAKEITLLGQIVDSWGHDLPGRPELADLLEAVDPTPGLLR
LRFLTSHPAWMTDRLIETVARLPRCQPEINLPVQAGSDRVLKLMRRGYTVARYKTLIARIRAAIPDISLTTDIIVGHPGE
TEDDFRQTMDLCAEIGFDKVHIAAFSARPGTRAAEQEQDPALAVPPAVKEERRRRLEQLQEQIATERMARFLGQTVEVLV
EGESKGKWRGRTPGNRLVFFSHPADLTGQLVPVKITATSPWSLQGVPQLDDSVNAQSKENGLVKAR

Sequences:

>Translated_466_residues
MQDVIFFDQRPRPDRDQTPRERRYYVWTVGCQMNISDSERLEAALQGVGYSPATRPEDASFIVLNSCSVRASAEERILGK
LGELVRVKRQHPDTRIVLWGCMVGPNNRSIFADQLPMVDHFVSPSAVDEVVALAPNPIYTLDEPALPVRDWSHPPVSVHV
PIQYGCNMTCSYCVIPLRRGRERSRPLAEIVEEVRRIVARGAKEITLLGQIVDSWGHDLPGRPELADLLEAVDPTPGLLR
LRFLTSHPAWMTDRLIETVARLPRCQPEINLPVQAGSDRVLKLMRRGYTVARYKTLIARIRAAIPDISLTTDIIVGHPGE
TEDDFRQTMDLCAEIGFDKVHIAAFSARPGTRAAEQEQDPALAVPPAVKEERRRRLEQLQEQIATERMARFLGQTVEVLV
EGESKGKWRGRTPGNRLVFFSHPADLTGQLVPVKITATSPWSLQGVPQLDDSVNAQSKENGLVKAR
>Mature_466_residues
MQDVIFFDQRPRPDRDQTPRERRYYVWTVGCQMNISDSERLEAALQGVGYSPATRPEDASFIVLNSCSVRASAEERILGK
LGELVRVKRQHPDTRIVLWGCMVGPNNRSIFADQLPMVDHFVSPSAVDEVVALAPNPIYTLDEPALPVRDWSHPPVSVHV
PIQYGCNMTCSYCVIPLRRGRERSRPLAEIVEEVRRIVARGAKEITLLGQIVDSWGHDLPGRPELADLLEAVDPTPGLLR
LRFLTSHPAWMTDRLIETVARLPRCQPEINLPVQAGSDRVLKLMRRGYTVARYKTLIARIRAAIPDISLTTDIIVGHPGE
TEDDFRQTMDLCAEIGFDKVHIAAFSARPGTRAAEQEQDPALAVPPAVKEERRRRLEQLQEQIATERMARFLGQTVEVLV
EGESKGKWRGRTPGNRLVFFSHPADLTGQLVPVKITATSPWSLQGVPQLDDSVNAQSKENGLVKAR

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=35, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=35, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI93277076, Length=473, Percent_Identity=28.1183932346723, Blast_Score=162, Evalue=9e-40,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=38.4787472035794, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1787057, Length=450, Percent_Identity=27.7777777777778, Blast_Score=165, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=31.0195227765727, Blast_Score=191, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI71996771, Length=296, Percent_Identity=27.7027027027027, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI21356207, Length=495, Percent_Identity=32.1212121212121, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI19922432, Length=467, Percent_Identity=29.3361884368308, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_CHLAA (A9WHB1)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001636012.1
- ProteinModelPortal:   A9WHB1
- GeneID:   5826878
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_2414
- HOGENOM:   HBG457663
- OMA:   SYHHLPQ
- ProtClustDB:   PRK14338
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52223; Mature: 52223

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDVIFFDQRPRPDRDQTPRERRYYVWTVGCQMNISDSERLEAALQGVGYSPATRPEDAS
CCCEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCC
FIVLNSCSVRASAEERILGKLGELVRVKRQHPDTRIVLWGCMVGPNNRSIFADQLPMVDH
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEECCCCCHHHH
FVSPSAVDEVVALAPNPIYTLDEPALPVRDWSHPPVSVHVPIQYGCNMTCSYCVIPLRRG
HCCCHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCEEEEEEEECCCCCEEHHEEEECCCC
RERSRPLAEIVEEVRRIVARGAKEITLLGQIVDSWGHDLPGRPELADLLEAVDPTPGLLR
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEE
LRFLTSHPAWMTDRLIETVARLPRCQPEINLPVQAGSDRVLKLMRRGYTVARYKTLIARI
EEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHCCCHHHHHHHHHHHH
RAAIPDISLTTDIIVGHPGETEDDFRQTMDLCAEIGFDKVHIAAFSARPGTRAAEQEQDP
HHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHCCCC
ALAVPPAVKEERRRRLEQLQEQIATERMARFLGQTVEVLVEGESKGKWRGRTPGNRLVFF
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCEEEEE
SHPADLTGQLVPVKITATSPWSLQGVPQLDDSVNAQSKENGLVKAR
ECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure
MQDVIFFDQRPRPDRDQTPRERRYYVWTVGCQMNISDSERLEAALQGVGYSPATRPEDAS
CCCEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCC
FIVLNSCSVRASAEERILGKLGELVRVKRQHPDTRIVLWGCMVGPNNRSIFADQLPMVDH
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEECCCCCHHHH
FVSPSAVDEVVALAPNPIYTLDEPALPVRDWSHPPVSVHVPIQYGCNMTCSYCVIPLRRG
HCCCHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCEEEEEEEECCCCCEEHHEEEECCCC
RERSRPLAEIVEEVRRIVARGAKEITLLGQIVDSWGHDLPGRPELADLLEAVDPTPGLLR
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEE
LRFLTSHPAWMTDRLIETVARLPRCQPEINLPVQAGSDRVLKLMRRGYTVARYKTLIARI
EEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHCCCHHHHHHHHHHHH
RAAIPDISLTTDIIVGHPGETEDDFRQTMDLCAEIGFDKVHIAAFSARPGTRAAEQEQDP
HHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHCCCC
ALAVPPAVKEERRRRLEQLQEQIATERMARFLGQTVEVLVEGESKGKWRGRTPGNRLVFF
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCEEEEE
SHPADLTGQLVPVKITATSPWSLQGVPQLDDSVNAQSKENGLVKAR
ECCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA