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The map label for this gene is glcK [H]

Identifier: 222525775

GI number: 222525775

Start: 3176257

End: 3177228

Strand: Reverse

Name: glcK [H]

Synonym: Chy400_2528

Alternate gene names: 222525775

Gene position: 3177228-3176257 (Counterclockwise)

Preceding gene: 222525776

Following gene: 222525774

Centisome position: 60.3

GC content: 58.64

Gene sequence:

>972_bases
ATGAAAGATCTGCCTGCCCTTATTCGAGAACGGTTGACGCAGAACCTCTGGGGCGTCCTTGGGAATCAAGGCTACATCCT
TGGGATCGACCTGGGCAGTTACGGATTACGAGCTGCATTGGTCGACTTGCAGCACCACACCTACCAGAATGCCGCTCTCG
AGTCGATTGATCATGAAGACCCAGAGACAACGGTACGCCGGGTTATCGATCTGGCGCGAGATTTGATGGCGCAGGAAGGT
GTTACCGTCGAGCGCCTGGTACGGGTTGGCGTAGGCTTTAGCGGCCCGGTTGACCTCCGCCATGGCACGGTACGGCTGGC
CCCACGGCGACCGGGGTGGGAAAATTACCCCTTGCAAGAGCGTTTCGAGCAGGCGTTTGATGCCGTGACGCTCATTGACA
ACGATGCGAATCTGATCGCCCTGGGAGAGGCAACCTTTGGTGTCGGCAAAGGATGCCAGCATCTATTTTACTTTCACCTG
AGCAGTGGCGTTGGCGGGGGCGCAGTGCTGAACGGTCGCCTGTACCATGGCGCCCACTCAATGGCCGGCGAAATCGGGCA
TGCAGTGGTTGCCCACGGCTGGGATGGCGATGGTCGCCCGGAGACCCTGGAAGAGCTGGTTTCGATAGGAGGATTAATCC
GCCGGGCAGCCGCCGCAGGGCTTGCCGCGGATCGACTGGAAGATATTTTTAGCGATCATCCGATTGCCCGCAAGGTAGTA
CAGCATACCGTTGATCTCATGGCCACCCGGATTGCGCAAGTGATTGCACTGCTCGATCCAGAGATGGTTGTCCTGGGAGG
AATCGTTGTGCGGATCGGTGGTGATCGCTTCGTGGAAGCGATTGCGACCAGAGTGAACGACTTCATCGCCCCACAGTTCG
CCCGTCCCGTACCGGTGGTAGCGTCAATCCTTGGCCCCGATAGCGTCGCAATTGGCGCTGTCGCGATGGCACTCGATAGC
CTGTCGGATTGA

Upstream 100 bases:

>100_bases
GAACCAACACCACAACGGACTGCGCGCAGAGAACATGATGTGCTATAATCTCACAACACCGGTGCCGACCATTCGCTGAA
ATGCAGAGAGGAACAGCCCT

Downstream 100 bases:

>100_bases
GCTTCCCATTTGCATATCGCGCTGAGCAATGCTACAATAGGATAAGCTATATCGTACACCCTACGGATAGCAGGGAGGCA
AGTTGCGCCTCTCTGTTTCC

Product: ROK family protein

Products: NA

Alternate protein names: Glucose kinase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKDLPALIRERLTQNLWGVLGNQGYILGIDLGSYGLRAALVDLQHHTYQNAALESIDHEDPETTVRRVIDLARDLMAQEG
VTVERLVRVGVGFSGPVDLRHGTVRLAPRRPGWENYPLQERFEQAFDAVTLIDNDANLIALGEATFGVGKGCQHLFYFHL
SSGVGGGAVLNGRLYHGAHSMAGEIGHAVVAHGWDGDGRPETLEELVSIGGLIRRAAAAGLAADRLEDIFSDHPIARKVV
QHTVDLMATRIAQVIALLDPEMVVLGGIVVRIGGDRFVEAIATRVNDFIAPQFARPVPVVASILGPDSVAIGAVAMALDS
LSD

Sequences:

>Translated_323_residues
MKDLPALIRERLTQNLWGVLGNQGYILGIDLGSYGLRAALVDLQHHTYQNAALESIDHEDPETTVRRVIDLARDLMAQEG
VTVERLVRVGVGFSGPVDLRHGTVRLAPRRPGWENYPLQERFEQAFDAVTLIDNDANLIALGEATFGVGKGCQHLFYFHL
SSGVGGGAVLNGRLYHGAHSMAGEIGHAVVAHGWDGDGRPETLEELVSIGGLIRRAAAAGLAADRLEDIFSDHPIARKVV
QHTVDLMATRIAQVIALLDPEMVVLGGIVVRIGGDRFVEAIATRVNDFIAPQFARPVPVVASILGPDSVAIGAVAMALDS
LSD
>Mature_323_residues
MKDLPALIRERLTQNLWGVLGNQGYILGIDLGSYGLRAALVDLQHHTYQNAALESIDHEDPETTVRRVIDLARDLMAQEG
VTVERLVRVGVGFSGPVDLRHGTVRLAPRRPGWENYPLQERFEQAFDAVTLIDNDANLIALGEATFGVGKGCQHLFYFHL
SSGVGGGAVLNGRLYHGAHSMAGEIGHAVVAHGWDGDGRPETLEELVSIGGLIRRAAAAGLAADRLEDIFSDHPIARKVV
QHTVDLMATRIAQVIALLDPEMVVLGGIVVRIGGDRFVEAIATRVNDFIAPQFARPVPVVASILGPDSVAIGAVAMALDS
LSD

Specific function: Unknown

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=283, Percent_Identity=25.7950530035336, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786891, Length=322, Percent_Identity=24.5341614906832, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787363, Length=259, Percent_Identity=27.027027027027, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR004654 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 34674; Mature: 34674

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLPALIRERLTQNLWGVLGNQGYILGIDLGSYGLRAALVDLQHHTYQNAALESIDHED
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PETTVRRVIDLARDLMAQEGVTVERLVRVGVGFSGPVDLRHGTVRLAPRRPGWENYPLQE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCHHH
RFEQAFDAVTLIDNDANLIALGEATFGVGKGCQHLFYFHLSSGVGGGAVLNGRLYHGAHS
HHHHHHCEEEEEECCCCEEEECCCHHCCCHHHHHHHHEEECCCCCCCEEECCEEECCHHH
MAGEIGHAVVAHGWDGDGRPETLEELVSIGGLIRRAAAAGLAADRLEDIFSDHPIARKVV
HHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
QHTVDLMATRIAQVIALLDPEMVVLGGIVVRIGGDRFVEAIATRVNDFIAPQFARPVPVV
HHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCHHHHHHHHHHHHHHHCCHHCCCHHHH
ASILGPDSVAIGAVAMALDSLSD
HHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDLPALIRERLTQNLWGVLGNQGYILGIDLGSYGLRAALVDLQHHTYQNAALESIDHED
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PETTVRRVIDLARDLMAQEGVTVERLVRVGVGFSGPVDLRHGTVRLAPRRPGWENYPLQE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCHHH
RFEQAFDAVTLIDNDANLIALGEATFGVGKGCQHLFYFHLSSGVGGGAVLNGRLYHGAHS
HHHHHHCEEEEEECCCCEEEECCCHHCCCHHHHHHHHEEECCCCCCCEEECCEEECCHHH
MAGEIGHAVVAHGWDGDGRPETLEELVSIGGLIRRAAAAGLAADRLEDIFSDHPIARKVV
HHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
QHTVDLMATRIAQVIALLDPEMVVLGGIVVRIGGDRFVEAIATRVNDFIAPQFARPVPVV
HHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCHHHHHHHHHHHHHHHCCHHCCCHHHH
ASILGPDSVAIGAVAMALDSLSD
HHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]