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The map label for this gene is appA [H]

Identifier: 222525763

GI number: 222525763

Start: 3159429

End: 3161093

Strand: Direct

Name: appA [H]

Synonym: Chy400_2516

Alternate gene names: 222525763

Gene position: 3159429-3161093 (Clockwise)

Preceding gene: 222525762

Following gene: 222525765

Centisome position: 59.96

GC content: 56.22

Gene sequence:

>1665_bases
ATGGCGCGTCGTATTCGCTGGCAAATCGCGATTGCCCTCTTCGGCATTGCGCTGATAGTCGCTTTGTTTGGTCGACTCGC
CGCACTCAGTGCATCGACCGATAATCCGGCAACTGGTGGTGTGTATATTGAGGCTGTCGTCGGCTCGCCGATCACGCCCA
TTCCTCTGCTCAATGATCCGGTCGCCGATCCGGTTGGGCGCACGTTGATCAGCCTCCTCTTTGAAGGATTAACCCGTATT
GGGAGTGATGGCTTGATCGAACCTGCCCTGGCTGAAGGCTATACTGTCGATGCCACCGGCGAAATCTATACGTTCAACCT
GCGACCAGGGCTGCGCTGGCACGATGGTACCCCCATCACCGCTGACGATGTTGTTTTTACCTTTCGGACGTTGCAGACAT
TGGATGTAGCGGGTGAACCGGCACTTGCCGGCTTCTGGCGCACTGCTTTAATTGAGCGTGTCGATACTCGCACAGTACGG
GTGACGTTATCAGGGCCATTTGCTCCCTTTCCAAGCCTTGCCCGCGTGCCGATCCTCCCCGCTCATCTGCTCCGTGGTCT
TTCGCCTGCTGAATGGCCGGCCAGCGAGTTTGCCCGTCGTCTGGTGGGTAGTGGGCCGTTTCGGCTGGCTGAATTACGCT
CCGATGCGGCTATCTTGACCGCCAACCCGACATACTACAATGGTCGGCCCTTTCTCGATCAAATAGAGTTGCGGTTTGTA
AGTACCGCCGAAGCCGCGATTGCCGCTTTGTCACGTGGTGAAGTGAACGGCTTTGCAGAACGCTGGGGAACCAATCTTGC
AACTATCGATCTTCCTGGGGAAGAGCAGCAAATTGTCTTGCCGATGGATGAATATACCCTGCTCACATTCAATCTGCGTT
TGCCGTTGTTCCAGGATATTCCCCTGCGCCGGGCACTGGCACTCGGCCTTGATCGTGATGCTTTGATCGAGACGGTTGTT
AATGGGATGGCGCAGCCGATAGATACCCCTCTCCTGCCAGGTACCTGGGCCGATGATCCTGCCCTGCGTCGTATGCCGGC
TGAACCATCCGTAGCCGCCGAACGTCTGGCTGAAATTGATTTTGAACCGGGCGATGACGGTATTCGCCAGCGTGGAAGAC
AGCGTCTTAGCTTTACATTGCTGGTTGACCGCGATGAGCGGCGTGTAGCAGTGGCAGAGGCTATCGCTGCCCAGTGGCGA
TCTATCGGTGTGGAAGTGTTTGTTGAGTCGGTTGACGTCACAACTTTGACCGAGCGCCTACGCCAGGGCGATTTTATGGC
TGCCATCCATACCTGGACGCGGATCGGGCCTGACCCCGACCCCTATAGCCTCTGGCACTCCAGTCAGGCTGATGACGGCC
TCAATTATGCCGGATTAGAGGATAGTCGCATTGATACTTTACTCGAACAGGCGCGGGCTGAGCCTGAACTGATAGCCAGG
GCTGAGTTTTATCACGCTTTTCAACAGCGCTGGCTGGAACTGTCACCGGCAATTACCCTCTACCAGCCGATGTACGTGAT
GGTAACGACGGCAAACCTGCAAGGGCGCGCCTTCGCTAATCCCGATCTCGGCCCCCATACACTCTTCGGGATCGAGGATC
GTTTTCGTGATGTGCAGCGTTGGTTTGTGAATAGCTTCCGCCGTATCGAAGGTGATTTGCCGTAG

Upstream 100 bases:

>100_bases
CCGAGGCATCGAGAAGACACTGTATCAGGCCACGATTGCCCTTGGGGTTACCTTTCTGCTGTTGGCCCTGATCACGAGCC
TGCCGATTTTCAATTAGGGT

Downstream 100 bases:

>100_bases
AGAGGCTGTTTGCCGGTCTACATGTCTACACTTCCGCTCTAGTCAGATGGTGTATCGCCTGTCTCACAGCGACAGGCGAA
GATCGTATAGGTCAAACACC

Product: family 5 extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MARRIRWQIAIALFGIALIVALFGRLAALSASTDNPATGGVYIEAVVGSPITPIPLLNDPVADPVGRTLISLLFEGLTRI
GSDGLIEPALAEGYTVDATGEIYTFNLRPGLRWHDGTPITADDVVFTFRTLQTLDVAGEPALAGFWRTALIERVDTRTVR
VTLSGPFAPFPSLARVPILPAHLLRGLSPAEWPASEFARRLVGSGPFRLAELRSDAAILTANPTYYNGRPFLDQIELRFV
STAEAAIAALSRGEVNGFAERWGTNLATIDLPGEEQQIVLPMDEYTLLTFNLRLPLFQDIPLRRALALGLDRDALIETVV
NGMAQPIDTPLLPGTWADDPALRRMPAEPSVAAERLAEIDFEPGDDGIRQRGRQRLSFTLLVDRDERRVAVAEAIAAQWR
SIGVEVFVESVDVTTLTERLRQGDFMAAIHTWTRIGPDPDPYSLWHSSQADDGLNYAGLEDSRIDTLLEQARAEPELIAR
AEFYHAFQQRWLELSPAITLYQPMYVMVTTANLQGRAFANPDLGPHTLFGIEDRFRDVQRWFVNSFRRIEGDLP

Sequences:

>Translated_554_residues
MARRIRWQIAIALFGIALIVALFGRLAALSASTDNPATGGVYIEAVVGSPITPIPLLNDPVADPVGRTLISLLFEGLTRI
GSDGLIEPALAEGYTVDATGEIYTFNLRPGLRWHDGTPITADDVVFTFRTLQTLDVAGEPALAGFWRTALIERVDTRTVR
VTLSGPFAPFPSLARVPILPAHLLRGLSPAEWPASEFARRLVGSGPFRLAELRSDAAILTANPTYYNGRPFLDQIELRFV
STAEAAIAALSRGEVNGFAERWGTNLATIDLPGEEQQIVLPMDEYTLLTFNLRLPLFQDIPLRRALALGLDRDALIETVV
NGMAQPIDTPLLPGTWADDPALRRMPAEPSVAAERLAEIDFEPGDDGIRQRGRQRLSFTLLVDRDERRVAVAEAIAAQWR
SIGVEVFVESVDVTTLTERLRQGDFMAAIHTWTRIGPDPDPYSLWHSSQADDGLNYAGLEDSRIDTLLEQARAEPELIAR
AEFYHAFQQRWLELSPAITLYQPMYVMVTTANLQGRAFANPDLGPHTLFGIEDRFRDVQRWFVNSFRRIEGDLP
>Mature_553_residues
ARRIRWQIAIALFGIALIVALFGRLAALSASTDNPATGGVYIEAVVGSPITPIPLLNDPVADPVGRTLISLLFEGLTRIG
SDGLIEPALAEGYTVDATGEIYTFNLRPGLRWHDGTPITADDVVFTFRTLQTLDVAGEPALAGFWRTALIERVDTRTVRV
TLSGPFAPFPSLARVPILPAHLLRGLSPAEWPASEFARRLVGSGPFRLAELRSDAAILTANPTYYNGRPFLDQIELRFVS
TAEAAIAALSRGEVNGFAERWGTNLATIDLPGEEQQIVLPMDEYTLLTFNLRLPLFQDIPLRRALALGLDRDALIETVVN
GMAQPIDTPLLPGTWADDPALRRMPAEPSVAAERLAEIDFEPGDDGIRQRGRQRLSFTLLVDRDERRVAVAEAIAAQWRS
IGVEVFVESVDVTTLTERLRQGDFMAAIHTWTRIGPDPDPYSLWHSSQADDGLNYAGLEDSRIDTLLEQARAEPELIARA
EFYHAFQQRWLELSPAITLYQPMYVMVTTANLQGRAFANPDLGPHTLFGIEDRFRDVQRWFVNSFRRIEGDLP

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=457, Percent_Identity=23.8512035010941, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1787762, Length=479, Percent_Identity=25.678496868476, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1789397, Length=482, Percent_Identity=25.5186721991701, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87081878, Length=482, Percent_Identity=24.6887966804979, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787052, Length=519, Percent_Identity=25.2408477842004, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787495, Length=477, Percent_Identity=23.8993710691824, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787551, Length=455, Percent_Identity=23.0769230769231, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61314; Mature: 61182

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRIRWQIAIALFGIALIVALFGRLAALSASTDNPATGGVYIEAVVGSPITPIPLLNDP
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
VADPVGRTLISLLFEGLTRIGSDGLIEPALAEGYTVDATGEIYTFNLRPGLRWHDGTPIT
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEECCCCEEEEECCCCCEECCCCCCC
ADDVVFTFRTLQTLDVAGEPALAGFWRTALIERVDTRTVRVTLSGPFAPFPSLARVPILP
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCH
AHLLRGLSPAEWPASEFARRLVGSGPFRLAELRSDAAILTANPTYYNGRPFLDQIELRFV
HHHHCCCCCCCCCHHHHHHHHHCCCCEEEHHHCCCCEEEEECCCEECCCCCHHHHHEEEE
STAEAAIAALSRGEVNGFAERWGTNLATIDLPGEEQQIVLPMDEYTLLTFNLRLPLFQDI
HHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCEEEEECCCEEEEEEEECCCCCCCC
PLRRALALGLDRDALIETVVNGMAQPIDTPLLPGTWADDPALRRMPAEPSVAAERLAEID
CHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHCC
FEPGDDGIRQRGRQRLSFTLLVDRDERRVAVAEAIAAQWRSIGVEVFVESVDVTTLTERL
CCCCCHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHH
RQGDFMAAIHTWTRIGPDPDPYSLWHSSQADDGLNYAGLEDSRIDTLLEQARAEPELIAR
HCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHH
AEFYHAFQQRWLELSPAITLYQPMYVMVTTANLQGRAFANPDLGPHTLFGIEDRFRDVQR
HHHHHHHHHHHHHCCCCEEEECCEEEEEEECCCCCCEECCCCCCCCEEECHHHHHHHHHH
WFVNSFRRIEGDLP
HHHHHHHHHCCCCC
>Mature Secondary Structure 
ARRIRWQIAIALFGIALIVALFGRLAALSASTDNPATGGVYIEAVVGSPITPIPLLNDP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
VADPVGRTLISLLFEGLTRIGSDGLIEPALAEGYTVDATGEIYTFNLRPGLRWHDGTPIT
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEECCCCEEEEECCCCCEECCCCCCC
ADDVVFTFRTLQTLDVAGEPALAGFWRTALIERVDTRTVRVTLSGPFAPFPSLARVPILP
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCH
AHLLRGLSPAEWPASEFARRLVGSGPFRLAELRSDAAILTANPTYYNGRPFLDQIELRFV
HHHHCCCCCCCCCHHHHHHHHHCCCCEEEHHHCCCCEEEEECCCEECCCCCHHHHHEEEE
STAEAAIAALSRGEVNGFAERWGTNLATIDLPGEEQQIVLPMDEYTLLTFNLRLPLFQDI
HHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCEEEEECCCEEEEEEEECCCCCCCC
PLRRALALGLDRDALIETVVNGMAQPIDTPLLPGTWADDPALRRMPAEPSVAAERLAEID
CHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHCC
FEPGDDGIRQRGRQRLSFTLLVDRDERRVAVAEAIAAQWRSIGVEVFVESVDVTTLTERL
CCCCCHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHH
RQGDFMAAIHTWTRIGPDPDPYSLWHSSQADDGLNYAGLEDSRIDTLLEQARAEPELIAR
HCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHH
AEFYHAFQQRWLELSPAITLYQPMYVMVTTANLQGRAFANPDLGPHTLFGIEDRFRDVQR
HHHHHHHHHHHHHCCCCEEEECCEEEEEEECCCCCCEECCCCCCCCEEECHHHHHHHHHH
WFVNSFRRIEGDLP
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]