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The map label for this gene is comEC [H]

Identifier: 222525703

GI number: 222525703

Start: 3096705

End: 3098024

Strand: Reverse

Name: comEC [H]

Synonym: Chy400_2454

Alternate gene names: 222525703

Gene position: 3098024-3096705 (Counterclockwise)

Preceding gene: 222525705

Following gene: 222525702

Centisome position: 58.8

GC content: 58.18

Gene sequence:

>1320_bases
ATGCCCCGTCAGACTACGTGCCCTTTCTGTGGTGCAATGCTACGATCATCGGCCAGATTCTGTAGCATGTGCGGAAGAGA
CCTTCGTCGTCCAGCCACACCACGGCAACGTCCTGCCAGATCGCGTTTCCGGCTGAGCTGTGGTGGTCTGGTGATTGGCG
TGATCGGACTCATTCTGATCTGCGGCGCTCTCAACGCTCTCTTGCCAGACCGGGCCGTAACATCAGGTACGGCTGATCAA
GAGCGGCAACAATCTGTCGGCACGACAGGTAATGATACTCCAACCACACGACCACCAACCCCAACAGCCACGCAGGTTCC
ATCGGCTGCGCCGACCGTAACCGTCGCGGAACCCATTATCGCCGGGGAGCAACCCCTGGAAGTGCATGTGATTGATGTTG
GTCAGGGAGACAGCATTCTGATCCGCACCCCAGAGGGGAAAACGGCGCTGATCGATGGTGGATACGACAACGGCCTGGCA
CTTGCCTATCTCCGTGAGCAGGGGATCAGCCAGATTGACGTGATGGTAGCCTCGCATCCGCACGCCGACCACATTGGCGG
GCTGGTTGAAGTGCTGAGAGCGTTGCCGGTCAAAGGGTTCTGGACTTCCGGCGCAACCCATACCACCGGCACCTACGAAC
AGCTCCTCGATGCGATTGATGCGGCAAGGGTTCCGTATTATGAAGTGCAGCGCGGTGATACCATTCCGCTGGGTCGGTTA
AGCTTTGAGGTGCTGCACAGCAATCCCGATGCCGACGATCTCAACAACACCTCGGTCGTGCTGCGCCTGGCTTACGGGAA
TGTCTCGTTTCTCTTCACCGGCGATGTGGAGGCGGCTGCCGAGCTGGACATGCTATCAACAGTGCGTGAGCGTCTCAACG
CCACTATTCTGAAGGTCGCTCATCACGGATCGCGCACCTCTTCAACAGCCGGGTTTCTGGCGGCAGTCCAGCCGAGGATT
GCCGTCTACTCGGCTGGACGCGACAACAATTACGGTCATCCACACCGCGAAACCATTCAAGCCCTGCAGCAAATAGGGGC
CGAAGTGTACGGCACGGATGAACACGGCACTATCGTTATTGTCACCGACGGCGTTGACTACCAGATTCAAACCGGGTATG
ATCGGCCACCGACTGCTGCACCGGAAATGCCCGGTACTGCTCCTTCATCCAACAACACGACCCAGGGTGATCGTGATTGC
AGAAATTTTGCGACCCAGGCCGAAGCCCAGGCATTTTTCCTCGCCAACGGCGGTCCGGCCCGCGATCCACACCGGTTAGA
CGGTGACGACGATGGAATTGCCTGTGAAAGCCTCCCTTAA

Upstream 100 bases:

>100_bases
GCATTCCGTTGTGACCAGGTGATCCAGTATGTGCGACCTGCACAGTCCCGGCTAACACCTGTGCTACGTCAGAGTGATCG
TCAATGAAAGGGAGATAACG

Downstream 100 bases:

>100_bases
AACATGAAAGGAACTGCGGTATGGAAGAGGTTCGGTATGAGCAGGGCGTCATTGATCGCTTTGAAGGTGAATACGCAGTG
ATCCTGATCGGTCAAACCCA

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MPRQTTCPFCGAMLRSSARFCSMCGRDLRRPATPRQRPARSRFRLSCGGLVIGVIGLILICGALNALLPDRAVTSGTADQ
ERQQSVGTTGNDTPTTRPPTPTATQVPSAAPTVTVAEPIIAGEQPLEVHVIDVGQGDSILIRTPEGKTALIDGGYDNGLA
LAYLREQGISQIDVMVASHPHADHIGGLVEVLRALPVKGFWTSGATHTTGTYEQLLDAIDAARVPYYEVQRGDTIPLGRL
SFEVLHSNPDADDLNNTSVVLRLAYGNVSFLFTGDVEAAAELDMLSTVRERLNATILKVAHHGSRTSSTAGFLAAVQPRI
AVYSAGRDNNYGHPHRETIQALQQIGAEVYGTDEHGTIVIVTDGVDYQIQTGYDRPPTAAPEMPGTAPSSNNTTQGDRDC
RNFATQAEAQAFFLANGGPARDPHRLDGDDDGIACESLP

Sequences:

>Translated_439_residues
MPRQTTCPFCGAMLRSSARFCSMCGRDLRRPATPRQRPARSRFRLSCGGLVIGVIGLILICGALNALLPDRAVTSGTADQ
ERQQSVGTTGNDTPTTRPPTPTATQVPSAAPTVTVAEPIIAGEQPLEVHVIDVGQGDSILIRTPEGKTALIDGGYDNGLA
LAYLREQGISQIDVMVASHPHADHIGGLVEVLRALPVKGFWTSGATHTTGTYEQLLDAIDAARVPYYEVQRGDTIPLGRL
SFEVLHSNPDADDLNNTSVVLRLAYGNVSFLFTGDVEAAAELDMLSTVRERLNATILKVAHHGSRTSSTAGFLAAVQPRI
AVYSAGRDNNYGHPHRETIQALQQIGAEVYGTDEHGTIVIVTDGVDYQIQTGYDRPPTAAPEMPGTAPSSNNTTQGDRDC
RNFATQAEAQAFFLANGGPARDPHRLDGDDDGIACESLP
>Mature_438_residues
PRQTTCPFCGAMLRSSARFCSMCGRDLRRPATPRQRPARSRFRLSCGGLVIGVIGLILICGALNALLPDRAVTSGTADQE
RQQSVGTTGNDTPTTRPPTPTATQVPSAAPTVTVAEPIIAGEQPLEVHVIDVGQGDSILIRTPEGKTALIDGGYDNGLAL
AYLREQGISQIDVMVASHPHADHIGGLVEVLRALPVKGFWTSGATHTTGTYEQLLDAIDAARVPYYEVQRGDTIPLGRLS
FEVLHSNPDADDLNNTSVVLRLAYGNVSFLFTGDVEAAAELDMLSTVRERLNATILKVAHHGSRTSSTAGFLAAVQPRIA
VYSAGRDNNYGHPHRETIQALQQIGAEVYGTDEHGTIVIVTDGVDYQIQTGYDRPPTAAPEMPGTAPSSNNTTQGDRDCR
NFATQAEAQAFFLANGGPARDPHRLDGDDDGIACESLP

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG2333

COG function: function code R; Predicted hydrolase (metallo-beta-lactamase superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=232, Percent_Identity=31.4655172413793, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 46787; Mature: 46656

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQTTCPFCGAMLRSSARFCSMCGRDLRRPATPRQRPARSRFRLSCGGLVIGVIGLILI
CCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHEEEECCHHHHHHHHHHHH
CGALNALLPDRAVTSGTADQERQQSVGTTGNDTPTTRPPTPTATQVPSAAPTVTVAEPII
HHHHHHHCCCCHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHH
AGEQPLEVHVIDVGQGDSILIRTPEGKTALIDGGYDNGLALAYLREQGISQIDVMVASHP
CCCCCCEEEEEEECCCCEEEEECCCCCEEEEECCCCCCEEEEEHHHCCCCEEEEEEECCC
HADHIGGLVEVLRALPVKGFWTSGATHTTGTYEQLLDAIDAARVPYYEVQRGDTIPLGRL
CHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCE
SFEVLHSNPDADDLNNTSVVLRLAYGNVSFLFTGDVEAAAELDMLSTVRERLNATILKVA
EEEEEECCCCCCCCCCCEEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEHH
HHGSRTSSTAGFLAAVQPRIAVYSAGRDNNYGHPHRETIQALQQIGAEVYGTDEHGTIVI
HCCCCCCCCCHHHHECCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCEEEE
VTDGVDYQIQTGYDRPPTAAPEMPGTAPSSNNTTQGDRDCRNFATQAEAQAFFLANGGPA
EECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCC
RDPHRLDGDDDGIACESLP
CCCCCCCCCCCCEECCCCC
>Mature Secondary Structure 
PRQTTCPFCGAMLRSSARFCSMCGRDLRRPATPRQRPARSRFRLSCGGLVIGVIGLILI
CCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHEEEECCHHHHHHHHHHHH
CGALNALLPDRAVTSGTADQERQQSVGTTGNDTPTTRPPTPTATQVPSAAPTVTVAEPII
HHHHHHHCCCCHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHH
AGEQPLEVHVIDVGQGDSILIRTPEGKTALIDGGYDNGLALAYLREQGISQIDVMVASHP
CCCCCCEEEEEEECCCCEEEEECCCCCEEEEECCCCCCEEEEEHHHCCCCEEEEEEECCC
HADHIGGLVEVLRALPVKGFWTSGATHTTGTYEQLLDAIDAARVPYYEVQRGDTIPLGRL
CHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCE
SFEVLHSNPDADDLNNTSVVLRLAYGNVSFLFTGDVEAAAELDMLSTVRERLNATILKVA
EEEEEECCCCCCCCCCCEEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEHH
HHGSRTSSTAGFLAAVQPRIAVYSAGRDNNYGHPHRETIQALQQIGAEVYGTDEHGTIVI
HCCCCCCCCCHHHHECCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCEEEE
VTDGVDYQIQTGYDRPPTAAPEMPGTAPSSNNTTQGDRDCRNFATQAEAQAFFLANGGPA
EECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCC
RDPHRLDGDDDGIACESLP
CCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]