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The map label for this gene is murC [C]

Identifier: 222525661

GI number: 222525661

Start: 3039381

End: 3040781

Strand: Reverse

Name: murC [C]

Synonym: Chy400_2412

Alternate gene names: 222525661

Gene position: 3040781-3039381 (Counterclockwise)

Preceding gene: 222525662

Following gene: 222525648

Centisome position: 57.71

GC content: 60.67

Gene sequence:

>1401_bases
ATGTACCGTCTCCGCACAACCGCAGCACTCGCACTCGCTCGCACTGCCGGCACCCTCTCGCGACGGTTGGGATTAGGTGG
TGGTACCAGCCTTCCCGGCCAGGTCGCGCGCATCCTCGATCCGACCATTCTGACCAACTTCTGCACCGCTTTGCCCGAAG
GTGTGGCGTTGATCGCCGGTACCAATGGCAAGACGACCACCAGTCGCATGGTCGCTACAATTCTTGAGCAGGCTGGCAAA
CCACTGCTGCACAATCGCGCCGGCGCGAACCTGTTGCCCGGCCTGACCACAGTCGCCATTGCCGATGCCGACTGGCGTGG
ACGGCCACACGCACACCAGGCACTCTTCGAGACGGATGAAGCGGCGCTGCCCCAGGCCATCGCCGAGACACGACCACGCC
TGGTTGTGCTGCTGAACCTGTTTCGCGATCAGCTTGATCGCTACGGTGAGATCGACACCATCGCCAGCCATTGGCGCACA
GCGCTGCAAACGCTTCCCCCAACCAGCACCGTCGTCTTCAATGCCGACGATCCGGCACTGGCCAGCATCGTGCAAGGGTT
ATCGGCTCAGATTATCGCCTTTGGCCTGGCAGATGTTCGCCAGGCCACCGGAGCGGCGCTGCACATCGCCGACTCACAGT
TCTGTCATGTCTGCGGCCATCGCTATCACTACCAGCACGTGTTCTACGCCCACATCGGTCACTATGCCTGCCCGCAGTGC
GGTCAGCACCGTCCTGAACCTGACATTGCCCTCACCGTGCTTGAACCACGCGGACTTGATGGGAGTGACCTTCAGATCAC
ACATCCCGGCGGTACAGTCCAGTTCCGTTTACCACTACCCGGCCTCTACAATGCCCAGAACGCTCTGGCAGCCACAGCAG
CGACGCTGGCGTTAGGTATAACCCCTGCGACTATTAGCAGTGCCCTCCAGAACTTTCAGGCCGCTTTTGGTCGGATTGAA
CGAATTACTGCCGGCCCGAATGGGCCACCGCTCTTGATCGCGTTGATCAAAAACCCGGTCGGTGCTTCCGAGACGGTACG
GATGTTTACGACGGCCACCGACCAACCCCTGCATCTTTTGATTGCCATCAACGACCGTTTCGCCGACGGCACCGATGTCT
CGTGGTTGTGGGACGCCGATTTTGAACCGCTGGCACAGCGGGTTGCCCACGTCACCGTCAGTGGCACCCGCGCTGCCGAT
ATGGCCCTGCGTCTCGACTACGCCGGTGTGGCCGGTGAGGTTATCACCGTCATCGATGATCTCCCTGCTGCCCTGGATGC
CGCACTATCCAGACTGCCACCCGGTGAAACACTTGCCGTTCTGCCTACCTATACCGCCATGCTCGATTTACGGAGCGAAA
TCAGTCGCCGCGGTTGGGCCAAACCCTTTTGGGAAGCGTAA

Upstream 100 bases:

>100_bases
CCCCATCAGCATCGCTCTTTGCCAGCAGTGGCGATGACGATGACGACGATGACAATCCGCTGGCTGCTGCTCTGCGCAAA
GCAGGCTTGAGTTAAAAGCT

Downstream 100 bases:

>100_bases
CCGCGACGACCTGTGTGCTGGAGGCCGATGCGGTACACATCTGGCAGGAGAGAAAGCGTGGTACAGTTTCCAGACGGGAT
TGCCCGGAGACAAAGCCACG

Product: hypothetical protein

Products: ADP; H+; Phosphate; UDP-N-acetylmuramoyl-L-alanine [C]

Alternate protein names: Mur Ligase Family Protein; UDP-N-Acetylmuramyl Tripeptide Synthetase; Ligase; Mur Ligase; UDP-N-Acetylmuramoylalanine-D-Glutamate Ligase; Mur Ligase Middle Domain Protein; UDP-N-Acetylmuramate-Alanine Ligase; Conserved Domain Protein; UDP-N-Acetylmuramyl Tripeptide Synthase-Like Protein; UDP-N-Acetylmuramyl Tripeptide Synthetase MurC; Peptide Ligase; UDP-N-Acetylmuramyl Tripeptide Synthetase Like; Murein Ligase; UDP-N-Acetylmuramoyl-L-Alanyl-D-Glutamate-Lysine Ligase; Udp-N-Acetylmuramyl Tripeptide Synthetase; Cell Wall Biosynthesis Protein Mur Ligase Family; Mur Ligase Middle Domain-Containing Protein; UDP-N-Acetylmuramyl Peptide Synthetase

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MYRLRTTAALALARTAGTLSRRLGLGGGTSLPGQVARILDPTILTNFCTALPEGVALIAGTNGKTTTSRMVATILEQAGK
PLLHNRAGANLLPGLTTVAIADADWRGRPHAHQALFETDEAALPQAIAETRPRLVVLLNLFRDQLDRYGEIDTIASHWRT
ALQTLPPTSTVVFNADDPALASIVQGLSAQIIAFGLADVRQATGAALHIADSQFCHVCGHRYHYQHVFYAHIGHYACPQC
GQHRPEPDIALTVLEPRGLDGSDLQITHPGGTVQFRLPLPGLYNAQNALAATAATLALGITPATISSALQNFQAAFGRIE
RITAGPNGPPLLIALIKNPVGASETVRMFTTATDQPLHLLIAINDRFADGTDVSWLWDADFEPLAQRVAHVTVSGTRAAD
MALRLDYAGVAGEVITVIDDLPAALDAALSRLPPGETLAVLPTYTAMLDLRSEISRRGWAKPFWEA

Sequences:

>Translated_466_residues
MYRLRTTAALALARTAGTLSRRLGLGGGTSLPGQVARILDPTILTNFCTALPEGVALIAGTNGKTTTSRMVATILEQAGK
PLLHNRAGANLLPGLTTVAIADADWRGRPHAHQALFETDEAALPQAIAETRPRLVVLLNLFRDQLDRYGEIDTIASHWRT
ALQTLPPTSTVVFNADDPALASIVQGLSAQIIAFGLADVRQATGAALHIADSQFCHVCGHRYHYQHVFYAHIGHYACPQC
GQHRPEPDIALTVLEPRGLDGSDLQITHPGGTVQFRLPLPGLYNAQNALAATAATLALGITPATISSALQNFQAAFGRIE
RITAGPNGPPLLIALIKNPVGASETVRMFTTATDQPLHLLIAINDRFADGTDVSWLWDADFEPLAQRVAHVTVSGTRAAD
MALRLDYAGVAGEVITVIDDLPAALDAALSRLPPGETLAVLPTYTAMLDLRSEISRRGWAKPFWEA
>Mature_466_residues
MYRLRTTAALALARTAGTLSRRLGLGGGTSLPGQVARILDPTILTNFCTALPEGVALIAGTNGKTTTSRMVATILEQAGK
PLLHNRAGANLLPGLTTVAIADADWRGRPHAHQALFETDEAALPQAIAETRPRLVVLLNLFRDQLDRYGEIDTIASHWRT
ALQTLPPTSTVVFNADDPALASIVQGLSAQIIAFGLADVRQATGAALHIADSQFCHVCGHRYHYQHVFYAHIGHYACPQC
GQHRPEPDIALTVLEPRGLDGSDLQITHPGGTVQFRLPLPGLYNAQNALAATAATLALGITPATISSALQNFQAAFGRIE
RITAGPNGPPLLIALIKNPVGASETVRMFTTATDQPLHLLIAINDRFADGTDVSWLWDADFEPLAQRVAHVTVSGTRAAD
MALRLDYAGVAGEVITVIDDLPAALDAALSRLPPGETLAVLPTYTAMLDLRSEISRRGWAKPFWEA

Specific function: Cell Wall Formation. [C]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.8 [C]

Molecular weight: Translated: 49776; Mature: 49776

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRLRTTAALALARTAGTLSRRLGLGGGTSLPGQVARILDPTILTNFCTALPEGVALIAG
CCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCEEEEEC
TNGKTTTSRMVATILEQAGKPLLHNRAGANLLPGLTTVAIADADWRGRPHAHQALFETDE
CCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCH
AALPQAIAETRPRLVVLLNLFRDQLDRYGEIDTIASHWRTALQTLPPTSTVVFNADDPAL
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHH
ASIVQGLSAQIIAFGLADVRQATGAALHIADSQFCHVCGHRYHYQHVFYAHIGHYACPQC
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCHHHHEEEEHHHCCCCCCHH
GQHRPEPDIALTVLEPRGLDGSDLQITHPGGTVQFRLPLPGLYNAQNALAATAATLALGI
CCCCCCCCEEEEEECCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCC
TPATISSALQNFQAAFGRIERITAGPNGPPLLIALIKNPVGASETVRMFTTATDQPLHLL
CHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEE
IAINDRFADGTDVSWLWDADFEPLAQRVAHVTVSGTRAADMALRLDYAGVAGEVITVIDD
EEEECCCCCCCCCEEEECCCHHHHHHHHHEEEECCCCHHHEEEEEECCCCCCHHHHHHHH
LPAALDAALSRLPPGETLAVLPTYTAMLDLRSEISRRGWAKPFWEA
HHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MYRLRTTAALALARTAGTLSRRLGLGGGTSLPGQVARILDPTILTNFCTALPEGVALIAG
CCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCEEEEEC
TNGKTTTSRMVATILEQAGKPLLHNRAGANLLPGLTTVAIADADWRGRPHAHQALFETDE
CCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCH
AALPQAIAETRPRLVVLLNLFRDQLDRYGEIDTIASHWRTALQTLPPTSTVVFNADDPAL
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHH
ASIVQGLSAQIIAFGLADVRQATGAALHIADSQFCHVCGHRYHYQHVFYAHIGHYACPQC
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCHHHHEEEEHHHCCCCCCHH
GQHRPEPDIALTVLEPRGLDGSDLQITHPGGTVQFRLPLPGLYNAQNALAATAATLALGI
CCCCCCCCEEEEEECCCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCC
TPATISSALQNFQAAFGRIERITAGPNGPPLLIALIKNPVGASETVRMFTTATDQPLHLL
CHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEE
IAINDRFADGTDVSWLWDADFEPLAQRVAHVTVSGTRAADMALRLDYAGVAGEVITVIDD
EEEECCCCCCCCCEEEECCCHHHHHHHHHEEEECCCCHHHEEEEEECCCCCCHHHHHHHH
LPAALDAALSRLPPGETLAVLPTYTAMLDLRSEISRRGWAKPFWEA
HHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: (NH4)2SO4; Co2+; Mg2+; Mn2+ [C]

Kcat value (1/min): 1700 [C]

Specific activity: 2.94

Km value (mM): 0.22 {N-acetylmuramate}} 1 {dihydro-UDP-N-acetylmuramate}} 1.99 {L-Ser}} 1.2 {L-Ser}} 0.19 {beta-cyano-L-Ala}} 10 {Gly}} 7.89 {Gly}} 2.5 {Gly}} 70 {L-Val}} 0.173 {beta-chloro-L-Ala}} 62 {N-(2-aminopropionyl)piperidine}} 16

Substrates: L-Alanine; ATP; UDP-N-acetylmuramate [C]

Specific reaction: L-Alanine + ATP + UDP-N-acetylmuramate --> ADP + H+ + Phosphate + UDP-N-acetylmuramoyl-L-alanine [C]

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: 3, 3, 3-Trifluoro-DL-Ala; 3, 3-Difluoro-L-Ala; 3-Chloro-L-Ala; 3-Cyano-L-Ala; 3-Fluoro-L-Ala; Gly; KCl; L-2-Aminobutyrate; L-Allyl glycine; L-Cys; L-Lactate; L-Propargyl glycine; L-Ser; L-Vinyl glycine; N-Methyl -L-Ala; NaCl; NEM; p-Hydroxy mercuribenzoat

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA