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The map label for this gene is tcmA [H]

Identifier: 222525643

GI number: 222525643

Start: 3021234

End: 3022640

Strand: Direct

Name: tcmA [H]

Synonym: Chy400_2394

Alternate gene names: 222525643

Gene position: 3021234-3022640 (Clockwise)

Preceding gene: 222525642

Following gene: 222525644

Centisome position: 57.34

GC content: 60.84

Gene sequence:

>1407_bases
ATGAACGACCCTACCATCCGTTCGCCCTGGCTCGTCTTCGCCATCACCTGTCTGGCCGTTTTTGCTGTGATGCTGGACAG
TCTGGTGCTCCTGGTCGCTTTCCAGGCGATCCAGGCCAGCTTCGCCTCTGTTGATGTGGCTCAACTGTCCTGGGTGTTGA
ACGCTTATACCATTGTCTACGGTGCACTGCTCGTGCCTGCCGGTAGTCTGGCTGACCGGTTTGGACGGCGGCGTATGTTT
TTGTTGGGAGCAATCATCTTTACGCTTGCATCCCTGCTTTGCGGGATGGCATCCGAGATTAACTGGCTGATAGGCTTTCG
TGCGCTTCAAGCTCTCGGTGGCGCACTTCTCTCACCGGCGGCACTAGCCCTCGTGCTCGCCGCCTTCCCGATTGCCCGCC
GCACGCTGGCAGTGTCGCTTTGGGGTGCGGTTGGTGCGCTCGCAGTTGTCGCCGGGCCGCCGCTTGGTTCATGGGTTGTG
CAAGTCTTCGGCTGGCCGGGAATCTTCTTCCTCAATCTACCGATTGGGCTGCTGACAGTTGGTTTGGGCTGGCGAGTGAT
CGCCGAGTCACGCGACATGTCTGCCGCCGGTTGGCCCGATCTGCTAGGTGTGGCCCTGCTCTGTCTGAGTGCCGGCCTGC
TCACGGTTGGTCTGGTGCAAAGCGAGCATTGGGGATGGCAGTCCCCGGCAACCGTTACCGCCCTTTGCGCTGGTCTGGTC
GTTCTCGGCCTGTTTCTCCGTCGTTCACAGCTCGTGGCACAACCGGCCCTTGATCTGCGGCTCTTCCACGAGCGCAATTT
TCGGCTGGCGAACCTGGCGAATGTGCTTTTTGGCATTGCATTCACTGCGATGTTCTTTGGCTCGATCCAGTTTCTGACCC
GCGTCTGGGATTACAGTCTGCTCCAGGCCGGGCTGGCCATCACCCCTGGCCCCCTGATGGTGGTACTCGGCGCACCGCTG
GCCGGTCGGCTGGCGACACGCTGGGGGCAACGGATACTGCTCATTCCGGGGGGACTCTTCTACACTGCCGGGGCATTTCT
GCTGCTGGTGGGAGCCGGATCATATCCTCAGTTCCTGTCGGTATGGCTACCCGCAATGTTGCTCTCCGGTTTGGGGGTTG
CGCTCATCATTCCGCTCTTGAGCAGTGTAGCCGTTCAGCACTTGACGCCCGACCAGCTAGCCGGCGGGAGTGGGGTCTTC
CAGGCGTTACGCCAATTCGCCAGTGTTATCGGGGTCACTCTGAGTGTACTTCTGCTCGGTGCCACGCCTGCCACTGCTCA
GGCTTTCACACCGATCTTTGCCCTGATGATGGCTGGTGGTTTGGGGGTGACGCTGATGAGCTTTGGTCTGGCGACCGCGC
CGCAGCCCGTGATCGTACCCGCCGCTGTCCAGCCCACCGAACTGTAA

Upstream 100 bases:

>100_bases
GAGGAAACCCTGCTTCATCACGACATTGCGTCAATTCCAACCACTCTGATTCGCTCACGTTTCCATTCTCTATGCCGTGC
AAACTGAGAAAGGCATTCTC

Downstream 100 bases:

>100_bases
CGAGGATTGTTGTACAGCACGTCTGCTGTGCGACCACATTGTGGAGATATTTTTATGAATGCTACTCAAATCTTCGGCTT
GCAAGTCATCCTTAGCCTGG

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MNDPTIRSPWLVFAITCLAVFAVMLDSLVLLVAFQAIQASFASVDVAQLSWVLNAYTIVYGALLVPAGSLADRFGRRRMF
LLGAIIFTLASLLCGMASEINWLIGFRALQALGGALLSPAALALVLAAFPIARRTLAVSLWGAVGALAVVAGPPLGSWVV
QVFGWPGIFFLNLPIGLLTVGLGWRVIAESRDMSAAGWPDLLGVALLCLSAGLLTVGLVQSEHWGWQSPATVTALCAGLV
VLGLFLRRSQLVAQPALDLRLFHERNFRLANLANVLFGIAFTAMFFGSIQFLTRVWDYSLLQAGLAITPGPLMVVLGAPL
AGRLATRWGQRILLIPGGLFYTAGAFLLLVGAGSYPQFLSVWLPAMLLSGLGVALIIPLLSSVAVQHLTPDQLAGGSGVF
QALRQFASVIGVTLSVLLLGATPATAQAFTPIFALMMAGGLGVTLMSFGLATAPQPVIVPAAVQPTEL

Sequences:

>Translated_468_residues
MNDPTIRSPWLVFAITCLAVFAVMLDSLVLLVAFQAIQASFASVDVAQLSWVLNAYTIVYGALLVPAGSLADRFGRRRMF
LLGAIIFTLASLLCGMASEINWLIGFRALQALGGALLSPAALALVLAAFPIARRTLAVSLWGAVGALAVVAGPPLGSWVV
QVFGWPGIFFLNLPIGLLTVGLGWRVIAESRDMSAAGWPDLLGVALLCLSAGLLTVGLVQSEHWGWQSPATVTALCAGLV
VLGLFLRRSQLVAQPALDLRLFHERNFRLANLANVLFGIAFTAMFFGSIQFLTRVWDYSLLQAGLAITPGPLMVVLGAPL
AGRLATRWGQRILLIPGGLFYTAGAFLLLVGAGSYPQFLSVWLPAMLLSGLGVALIIPLLSSVAVQHLTPDQLAGGSGVF
QALRQFASVIGVTLSVLLLGATPATAQAFTPIFALMMAGGLGVTLMSFGLATAPQPVIVPAAVQPTEL
>Mature_468_residues
MNDPTIRSPWLVFAITCLAVFAVMLDSLVLLVAFQAIQASFASVDVAQLSWVLNAYTIVYGALLVPAGSLADRFGRRRMF
LLGAIIFTLASLLCGMASEINWLIGFRALQALGGALLSPAALALVLAAFPIARRTLAVSLWGAVGALAVVAGPPLGSWVV
QVFGWPGIFFLNLPIGLLTVGLGWRVIAESRDMSAAGWPDLLGVALLCLSAGLLTVGLVQSEHWGWQSPATVTALCAGLV
VLGLFLRRSQLVAQPALDLRLFHERNFRLANLANVLFGIAFTAMFFGSIQFLTRVWDYSLLQAGLAITPGPLMVVLGAPL
AGRLATRWGQRILLIPGGLFYTAGAFLLLVGAGSYPQFLSVWLPAMLLSGLGVALIIPLLSSVAVQHLTPDQLAGGSGVF
QALRQFASVIGVTLSVLLLGATPATAQAFTPIFALMMAGGLGVTLMSFGLATAPQPVIVPAAVQPTEL

Specific function: Resistance to tetracenomycin C by an active tetracenomycin C efflux system which is probably energized by transmembrane electrochemical gradients [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=414, Percent_Identity=29.2270531400966, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1788710, Length=441, Percent_Identity=28.1179138321995, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1790195, Length=433, Percent_Identity=27.2517321016166, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081983, Length=401, Percent_Identity=26.9326683291771, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788392, Length=419, Percent_Identity=27.6849642004773, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324954, Length=237, Percent_Identity=29.1139240506329, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=470, Percent_Identity=25.1063829787234, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49137; Mature: 49137

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDPTIRSPWLVFAITCLAVFAVMLDSLVLLVAFQAIQASFASVDVAQLSWVLNAYTIVY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GALLVPAGSLADRFGRRRMFLLGAIIFTLASLLCGMASEINWLIGFRALQALGGALLSPA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALALVLAAFPIARRTLAVSLWGAVGALAVVAGPPLGSWVVQVFGWPGIFFLNLPIGLLTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCHHHHHH
GLGWRVIAESRDMSAAGWPDLLGVALLCLSAGLLTVGLVQSEHWGWQSPATVTALCAGLV
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VLGLFLRRSQLVAQPALDLRLFHERNFRLANLANVLFGIAFTAMFFGSIQFLTRVWDYSL
HHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQAGLAITPGPLMVVLGAPLAGRLATRWGQRILLIPGGLFYTAGAFLLLVGAGSYPQFLS
HHCCCCCCCCCEEEEECCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCCHHHHH
VWLPAMLLSGLGVALIIPLLSSVAVQHLTPDQLAGGSGVFQALRQFASVIGVTLSVLLLG
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
ATPATAQAFTPIFALMMAGGLGVTLMSFGLATAPQPVIVPAAVQPTEL
CCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MNDPTIRSPWLVFAITCLAVFAVMLDSLVLLVAFQAIQASFASVDVAQLSWVLNAYTIVY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GALLVPAGSLADRFGRRRMFLLGAIIFTLASLLCGMASEINWLIGFRALQALGGALLSPA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALALVLAAFPIARRTLAVSLWGAVGALAVVAGPPLGSWVVQVFGWPGIFFLNLPIGLLTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCHHHHHH
GLGWRVIAESRDMSAAGWPDLLGVALLCLSAGLLTVGLVQSEHWGWQSPATVTALCAGLV
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VLGLFLRRSQLVAQPALDLRLFHERNFRLANLANVLFGIAFTAMFFGSIQFLTRVWDYSL
HHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQAGLAITPGPLMVVLGAPLAGRLATRWGQRILLIPGGLFYTAGAFLLLVGAGSYPQFLS
HHCCCCCCCCCEEEEECCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCCHHHHH
VWLPAMLLSGLGVALIIPLLSSVAVQHLTPDQLAGGSGVFQALRQFASVIGVTLSVLLLG
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
ATPATAQAFTPIFALMMAGGLGVTLMSFGLATAPQPVIVPAAVQPTEL
CCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1592819 [H]